Receptor Modulators

ABSTRACT

Method of identifying a modulator of CD28 comprising comparing a structural model of a candidate modulator with a structural model of CD28 to thereby determine whether the modulator will bind to CD28, wherein the structural model is derived from, or comprises, structural coordinates of a crystal of: (i) CD28, (ii) a fragment of CD28, or (iii) a homologue of (i) or (ii). The crystal of CD28 in a soluble form complexed with the Fab fragment of a mitogenic (superagonistic) antibody has been obtained and used for the determination of the 3D-structure of the receptor. The application also relates to modulators of superagonistic signalling for any receptor of the CD28 family, i.e. to superagonistic antibodies and chimeric proteins thereof, and to the screening of the superagonistic modulators. In the methods of screening, the binding of the candidate modulators to a portion of the receptor proximal to the cell membrane is investigated.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation of U.S. Ser. No. 10/585,491, filedJun. 15, 2007, which is the U.S. national stage application ofInternational Patent Application No. PCT/GB2005/000099, filed Jan. 10,2005, which claims the benefit of U.S. Provisional Patent ApplicationNo. 60/536,354, filed Jan. 14, 2004, the disclosures of which are herebyincorporated by reference in their entireties, including all figures,tables and amino acid or nucleic acid sequences.

FIELD OF THE INVENTION

The present invention relates to use of a crystal structure to obtainmodulators of a cell surface receptor and to the generation oftherapeutic antibodies and chimeric proteins that bind a particularclass of signalling receptors.

BACKGROUND OF THE INVENTION

CD28 is present on the surface of T cells and plays an important role intheir activation. Signal transduction occurs through CD28 after it isactivated (triggered) by binding to its ligand. CD28 activation isdependent on phosphorylation of its cytoplasmic domain. CD28 does nothave intrinsic phosphorylation activity but instead is dependent on anextrinsic kinase, e.g. p56lck.

SUMMARY OF THE INVENTION

The invention relates to the obtaining of the structure of CD28. Thiswas done by crystallizing a CD28/Fab fragment complex, subjecting it toX-ray diffraction and deriving the structural coordinates from thediffraction measurements. The Fab fragment is from an antibody that hassuperagonist activity towards CD28, i.e. is able to cause activation ofCD28 without the need for a T cell receptor-derived signal. Conventionalantibodies that activate CD28 need an additional signal generated by theT cell receptor. The deduced structure allows modulators of CD28signalling to be obtained which can in turn be used to modulate theimmune system.

Accordingly the invention provides a method of identifying a modulatorof CD28 comprising comparing a structural model of a candidate modulatorwith a structural model of CD28 to thereby determine whether themodulator will bind to CD28, wherein the structural model is derivedfrom, or comprises, structural coordinates of a crystal of: (i) CD28,(ii) a fragment of CD28, or (iii) a homologue of (i) or (ii).

In addition the invention relates to antibodies and chimeric proteinsthat are capable of being superagonists of particular receptors bypreferentially excluding phosphatases (as opposed to kinases) from thevicinity of the said receptor.

Accordingly the invention provides an antibody that causessuperagonistic signalling of a cell surface receptor, wherein saidantibody binds to the extracellular portion of the receptor at amembrane proximal region and said receptor comprises a cytoplasmicdomain which is dependent on an extrinsic protein kinase, wherein saidantibody does not bind only the C′-D loop of human CD28.

In addition the invention provides a chimeric protein that causessuperagonistic signalling of a cell surface receptor, which chimericprotein comprises:

(i) sequence representing a fragment of a ligand of the receptor, or ahomologue of such a fragment, wherein the fragment or homologue iscapable of binding to the extracellular portion of the receptor at amembrane proximal region, and

(ii) an Fc region of an antibody,

wherein said receptor comprises a cytoplasmic domain which is dependenton an extrinsic protein kinase.

Further the invention provides a chimeric protein that causessuperagonistic signalling of a first cell surface receptor, whichchimeric protein comprises two Fv regions of an antibody that may be thesame or different, wherein at least one of the Fv regions is capable ofbinding to said first receptor, and the other Fv region binds to asecond cell surface receptor expressed on another cell, wherein saidfirst receptor comprises a cytoplasmic domain which is dependent on anextrinsic protein kinase, and the first receptor can be identical to thesecond receptor.

DESCRIPTION OF THE DRAWINGS

FIG. 1 shows mechanisms of differential triggering of extrinsickinase-dependent receptors, by superagonistic agents in vitro and invivo.

A. In vitro superagonistic signalling, giving the example of CD28antibodies. The basic signalling principle is as follows. Antibodiesraised against CD28 bind distally (“conventional” antibodies, left) ormembrane-proximally (“superagonists”, right), as indicated by thestructure of the CD28-5.11A1 antibody-Fab complex. The antibodies holdthe cell surface at certain distances from an immobilising substrate: inthis case plastic, and as shown in parts B, C and D of the figure, Fcreceptor- or other receptor-bearing cells. For superagonistic antibodiesthis distance is typically 150-200 Å, whereas for costimulatoryantibodies, it is considerably larger.

The induced proximity of the membrane and the immobilising substrate inthe region of the immobilised antibody and receptor will lead to thedifferential steric exclusion, from the immediate vicinity of thereceptor, of other molecules whose extracellular domains are comparablein size or larger than CD28-antibody complexes, such as the tyrosinephosphatase, CD45. In contrast, tyrosine kinases, e.g. p56lck, will beunaffected because they are small and/or attached to the inner leafletof the membrane. The result is that, overall, the phosphorylation ofCD28 by the kinases will be favoured over its de-phoshorylation byphosphatases, with the net increase in phosphorylation amounting toreceptor triggering. Superagonists are more potent than conventionalantibodies because they bind epitopes close to the membrane rather thanat the “top” of the molecule, leading to more efficient exclusion of,e.g., CD45, and therefore a larger increase in the net phosphorylationof CD28.

B. In vivo superagonistic signalling, giving the example of CD28antibodies. The binding of the antibody to the membrane-proximal regionof CD28 on a T cell, and to the Fe receptor of, e.g. an antigenpresenting cell, forces the membranes of the two cells into closeproximity (150-200 Å). This in turn excludes CD45 from the immediatevicinity of CD28 as described in A, leading to signalling by CD28.

C. In vivo superagonistic signalling, giving the example of a chimeric,ligand-based agent. The chimera consists of a receptor-binding region ofthe ligand of the receptor, fused to the Fc region of an antibody. Thebinding of the ligand portion of the chimera to the receptor, and of theFc region of the chimera to the Fc receptor of, e.g. an antigenpresenting cell, forces the membranes of the two cells into closeproximity (150-200 Å). This in turn excludes CD45 from the immediatevicinity of the receptor as described in A, leading to signalling by thereceptor.

D. In vivo superagonistic signalling, giving the example of a chimeric,Fv-based agent. The chimera consists of the receptor-binding Fv regionof one antibody, fused to the Fv region of a second antibody reactivewith another receptor on a second cell. The binding of the chimera toboth receptors forces the membranes of the two cells into closeproximity (150-200 Å). This in turn excludes CD45 from the immediatevicinity of the receptor as described in A, leading to signalling by thereceptor.

FIG. 2 shows the method for identification of binding sites forsuperagonist antibodies of receptors raised against example receptors.

DESCRIPTION OF THE SEQUENCES MENTIONED HEREIN

SEQ ID NO:1 shows the amino acid sequence of CD28.

SEQ ID NO:2 shows the sequence of the CD28/Fc fusion protein used toexpress and dimerise CD28.

DETAILED DESCRIPTION OF THE INVENTION The CD28 Protein

Many of the different aspects of the invention discussed herein refer toCD28. It is to be understood that references to CD28 herein also include(i) a homologue of CD28, or (ii) a fragment of CD28 or the homologue, or(iii) a fusion protein comprising CD28, (i) or (ii), unless the contextrequires otherwise. The homologue and/or fragment of CD28 may be ofparticular lengths, as discussed below, or may have the binding orfunctional properties of naturally occurring (native) CD28, such as theability to bind a cell membrane and/or bind to B7-1 or B7-2. Thehomologue and/or fragment may comprise the extracellular domain of CD28.The homologue and/or fragment may comprise, or essentially consist of,the fragment of CD28 present in the fusion protein of SEQ ID NO:2. Thehomologue and/or fragment may have the ability to transduce a signal tothe cytoplasm of a T cell.

The CD28 may be of any species of animal, such as a mammalian or avianCD28. The CD28 is preferably a human CD28, for example as shown in SEQID NO:1. The CD28 protein may be present in particular forms, forexample which aid expression and/or crystallization. Thus the CD28 maybe fully glycosylated, partially glycosylated or lack glycosylationand/or have a reduced and alkylated stalk region.

The CD28 Crystal

The crystal of CD28 generally comprises CD28 present in a regularrepeating array. As mentioned above the term “CD28” includes fragmentsand/or homologues. Preferred fragments or homologues present in thecrystal comprise the extracellular domain of native CD28. In the crystalthe CD28 is preferably in the form of a monomer. The crystal may be ofCD28 bound to another moiety. Such a moiety may be an antibody specificfor CD28, including fragments/derivatives of the antibody (as furtherdiscussed below), which bind CD28. In a preferred embodiment the crystalis of CD28 bound to the Fab fragment of an antibody. The crystal maycomprise CD28 in a form that aids crystallization, and thus the CD28 maybe fully glycosylated, partially glycosylated or lack glycosylationand/or have a reduced and alkylated stalk region. In one embodiment thecrystal has the coordinates shown in Table 4.

The crystal is generally obtained by providing a solution that comprisesCD28 and optionally a moiety that binds to CD28, such as an antibodyfragment, and subjecting the solution to conditions that allow thecrystal to form. The CD28 which is to be crystallized is generallyobtained by recombinant expression, optionally in the form of a fusionprotein. The fusion protein may comprise CD28 and a polypeptide sequencewhich forms a homodimer. Such a fusion protein aids the formation of aCD28 homodimer. Preferably the fusion protein comprises the sequence ofthe Fc region of an antibody. The fusion protein may be cleaved beforecrystallisation to separate CD28 from the other polypeptide sequence,for example by thrombin.

The CD28 may be expressed in any suitable cell that is able to expresslarge amounts of CD28, such as a Chinese hamster ovary (CHO) cell.

The CD28 may be further treated in order to aid crystallization. Bindingto an antibody fragment, such as a Fab fragment, may be used to preventthe N-linked glycans present on the fully glycosylated form of CD28 frominhibiting the crystallisation of the protein. In one embodiment theantibody (or fragment thereof) is a superagonistic antibody, which mayhave any of the properties of the superagonistic antibodies mentionedherein. Thus, in one embodiment the antibody (or fragment thereof) bindsto a loop region in the extracellular membrane proximal region of CD28,such as the C′-D loop (said loop being defined for example as defined inUS-A1-2003/0166860 as the sequence from amino acid positions 52 to 66CD28 represented by the sequence GNYSQQLQVYSKTGF) (SEQ ID NO: 58).

The treatment may comprise reduction of the interchain disulphide bondsin the stalk-like region of CD28, e.g. using dithioreitol (DTT). Thereduced cysteines may then be inactivated, for example by alkylation(typically the alkyl moiety has 2 to 6, preferably 2, carbons). Thealkylation may be performed using iodoacetamide.

Crystallisation is typically carried out at 15 to 25° C., such as at 17to 19° C., preferably 18° C. Magnesium formate and polyethylene glycol(PEG) may be used as precipitating agents. Preferably precipitation iscarried out using 0.15 to 0.25 M magnesium formate (such as 0.2 Mmagnesium formate) and 15 to 25% PEG 3350 (such as 20% PEG 3350).

In the work described in the Examples it was found that in order tocrystallise CD28 this protein had to be expressed in the form of afusion protein with a second protein capable of forming a homodimer (theFc region of an antibody), the fully glycosylated form of CD28 needed tobe complexed with an Fab fragment of an antibody in order that theN-linked glycans did not interfere with crystallisation, and thedisulphide bonds in the stalk region of CD28 needed to be reduced andalkylated so that the stalk did not interfere with crystallisation. Thusin a preferred embodiment the method of obtaining a crystal of CD28(including fragment and/or homologue thereof) comprises

-   (a) expressing CD28 in the form of a fusion protein with a second    protein that is able to form a homodimer, wherein the presence of    the second protein in the fusion protein causes CD28 to dimerise,-   (b) cleaving the second protein from the fusion protein,-   (c) reducing and alkylating the disulphide bond present in the    stalk-like region of CD28, and-   (d) crystallising CD28 bound to the Fab fragment of an antibody.

Identifying a Modulator of CD28

The invention provides a method of identifying a modulator using thestructural coordinates determined from the above-mentioned crystal ofCD28. The structural coordinates used in the method may be in the formof a structural model, such as a three dimensional representation of thestructure or a pharmacophore. The coordinates/model typically compriseinformation relating to the identity of each atom (i.e. whether it isnitrogen, oxygen, hydrogen etc.) and its three dimensional location(normally defined by three spatial coordinates) in the structure. Themodel may also comprise additional information relevant to obtainingmodulators, such as the electronic charge at different locations in thestructure or information concerning whether or not the bonds in thestructure can be rotated.

The coordinates/model used in the method typically comprise a specificregion of the surface of CD28 corresponding to the site where it isdesired for the modulator to bind. Such a site typically comprisesGlu-32, Arg-34, Tyr-51, Glu-97, Met-99, Tyr-100, Pro-101, Pro-102,Pro-103, Tyr-104 and Leu-105, or a part thereof which comprises one ormore of these amino acids. Preferred modulators bind to such a site orpart thereof. In one embodiment the modulator is able to inhibit bindingof another moiety to this site. Such a moiety may be an antibody whichis specific to the site.

In the method the coordinates/model of CD28 are compared to thestructural coordinates/model of a candidate modulator to determinewhether or not the candidate modulator will bind to CD28. The comparisonmay be performed by any suitable means, such as the methods described orreferenced in Lyne (2002) Drug Discovery Today 7, 1047-1055. Thus one ormore of the algorithms described in this document may be used, such asone or more of Dock, FlexX, FlexE, Slide, Fred, Gold, Glide, AutoDock,LigandFit, ICM, QXP, Amber, CHARMM, SCORE, VALIDATE, Chemscore, Ludi,PLP, PMF, Bleep, SmoG, ZAP, VIDA, GRID, MCSS, Superstar and ROCS.

The method typically comprises deducing one or more ways of fitting(docking) a candidate modulator with CD28 followed by an evaluation(scoring) of the fit. The evaluation may comprise deducing the bindingenergy between CD28 and the modulator. This may be done based on theinteratomic distances between the atoms involved in binding or byanalysis of the force fields of CD28 and the modulator. In oneembodiment the evaluation comprises comparing the similarity between thefit between CD28 and the candidate modulator and the fit between one ormore other proteins and their ligands. Thus the evaluation may comprisecomparison with a database of structures of proteins fitted/bound toligands.

A candidate modulator which has been selected computationally asdiscussed above may be physically tested to determine whether or not itis able to bind or modulate CD28. Any suitable binding or activity assaymay be used. The binding assay may measure the extent of direct bindingbetween the candidate modulator and CD28 or instead be in the form of acompetition assay. The binding assay may comprise/use of any of thefollowing:

-   (a) an assay of binding to CD28 (which may be immobilized), for    example in which-   (i) the inhibition of B7-1 binding to the CD28 (a soluble form of    B7-1 may be used) in the presence of the candidate modulator is    measured,-   (ii) inhibition of the binding of an antibody (that binds to the    ligand binding face of CD28) to the CD28 in the presence of the    candidate modulator is measured,-   (b) a scintillation proximity assay (SPA) in which (i) or (ii) above    are measured,-   (c) an ELISA assay in which (i) or (ii) above are measured.

The activity assay may test the effect of the candidate modulator on theability of B7-1 or B7-2 to activate CD28. In such an assay, B7-1 or B7-2may be present on a natural or artificial antigen-presenting cell andCD28 may be present on a T cell. CD28 activity may be detected bymeasuring the extent of T cell activation, for example by determiningthe extent of T cell proliferation (e.g. thymidine incorporation) orgene expression in the T cell (e.g. with microarrays).

The candidate modulator may be tested using the assays described inGreen N J, Xiang J, Chen J, Chen L, Davies A M, Erbe D, Tam S, Tobin JF. (2003) Structure-activity studies of a series ofdipyrazolo[3,4-b:3′,4′-d]pyridin-3-ones binding to the immune regulatoryprotein B7.1. Bioorg Med Chem. 11, 2991-3013 or Erbe D V, Wang S, XingY, Tobin J F. (2002) Small molecule ligands define a binding site on theimmune regulatory protein B7.1. J Biol Chem. 277, 7363-8.

The Antibody and Chimeric Protein of the Invention

The term “antibody” as used herein is understood to also includefragments and derivatives of the antibody which retain binding ability,unless the context requires otherwise. Such fragments/derivativesinclude Fv, F(ab′) and F(ab′)₂ fragments, as well as single chainantibodies, camelid antibodies and similarly acting proteins.

The antibody of the invention may be of any species, such as a mammalianor bird antibody, preferably a rodent (such as mouse) or primate (suchas human) antibody. The antibody may be a chimeric antibody, aCDR-grafted antibody or a humanised antibody. The antibody may bemonoclonal or polyclonal. The antibody is preferably an IgG antibody.

The invention also provides two types of chimeric protein that bind to areceptor. One type of chimeric protein comprises the sequence of afragment of a ligand of the receptor, or sequence that is homologous tosuch a fragment. The sequence is capable of binding to an extracellularregion of the receptor, as discussed further below. The chimeric proteinalso comprises the Fc region of an antibody, such as that of any of thetypes of antibody discussed above. Where the chimeric protein isadministered to an individual the Fe region may be of an antibody of thesame species as the individual.

The second type of chimeric protein provided by the invention comprisestwo Fv regions of an antibody. The Fv regions may be the same ordifferent. One of the Fv regions is capable of binding to theextracellular region of the receptor (the “first” receptor). The otherFv region may bind the extracellular region of a “second” apposingreceptor, this being the same type of receptor as the first receptor(with the second Fv binding at a location which is the same or differentfrom the first Fv region) or an entirely different cell surfacereceptor, for example in the case where the first and second receptorsare each expressed on the surfaces of two cells capable ofinteracting/contacting each other. Such cells may be any of the types ofcell mentioned herein, including T cells.

In one embodiment the second Fv region of the second type of chimericprotein binds to a protein expressed on the surface of T cells, such asthe T cell receptor, CD2, CD4, CD5, CD8, CD52 or CS1. The second Fvregion may bind proteins expressed on the surface of other cells, suchas CD48, CD58, CD59, B7-I or B7-2. The term “receptor” when used in thepresent context refers to a protein expressed on surface of a cell(which may or may not be one which is capable of signal transduction,for example).

The antibody and chimeric protein of the invention are able to causesuperagonistic signalling of a cell surface receptor when they bind tothe receptor, typically according to the mechanism illustrated inFIG. 1. Thus the antibody or chimeric protein is able to causeactivation of the receptor (i.e. cause the receptor to transduce asignal to the cytoplasm of the cell) without the need for additionalcostimulus of the receptor or cell. As mentioned above, in oneembodiment the second type of chimeric protein comprises Fv regions ableto bind to different types of receptors. Such a chimeric protein ispreferably able to induce superagonistic signalling by both of thereceptors which it binds.

The antibody and both types of chimeric protein bind the extracellularportion of the receptor at a membrane proximal region of the receptor,typically to a region of the receptor which is within 75 Å of the cellmembrane, such as within 60 Å, 50 Å or 40 Å of the cell membrane.However the second type of chimeric protein of the invention typicallyalso binds the extracellular portion of the second apposing receptor (asdefined above), within 75 Å of the cell membrane, such as within 60 Å,50 Å or 40 Å of the cell membrane.

Generally the antibody or chimeric protein will be capable of bindingthe native form of the receptor (at the extracellular regions discussedabove) when the receptor is present on the surface of the cell where itoccurs naturally.

The antibody or chimeric protein cause superagonistic signalling bysterically hindering the access of phosphatases (which tend to be largeproteins), such as CD45, to the receptor (i.e. hindering contact of thephosphatase and receptor). In the case of the antibody or first type ofchimeric protein such steric hindrance may be caused by the antibodybinding to the receptor at one end (through the Fab of the antibody ofthe invention or the ligand sequence of the first type of chimericprotein) and typically binding to a protein on the surface of anothercell (e.g. due to the Fc region of the antibody or chimeric proteinbinding to an Fc receptor expressed on a second cell), thus bringing thecell membrane of the two cells into close proximity in the region of thereceptor.

For the second type of chimeric protein, one of the Fv fragments of thechimeric protein will bind to the receptor, and in the case where theother Fv fragment is specific for a second type of receptor expressed onanother cell, the other Fv fragment will bind to the second receptor andthus bring the cell membranes of the two cells into close proximity inthe region of the first receptor.

For the antibody and both types of chimeric protein, the closeproximities of the cell membranes in the region of the first receptorwill sterically hinder phosphatases from accessing the receptor, leadingto signal transduction or “triggering” of the receptor. The antibody orchimeric protein are typically capable of bringing the cell membranes oftwo cells within 200 Å of each other, such as within 180 Å, 150 Å or 120Å of each other.

The antibody or chimeric protein will preferably bind orthogonally tothe main axis of the domain of the receptor which is bound, such as atmore than 60 degrees from the main axis of the bound domain of thereceptor, for example at more than 70, 80 or 90 degrees. The antibody orchimeric protein will preferably lie substantially parallel to the cellsurface when bound to the receptor (to ensure the membranes are broughtinto close proximity).

The antibody or chimeric protein will bind to an extracellular region ofthe receptor, generally to amino acids of the receptor that are exposedat the surface of the protein. The amino acids in the receptor that arebound may be present in a loop region or a β-strand of the receptor.Preferred loops and strand sequences are shown for key examples inTable 1. The antibody or chimeric protein may bind to any of theepitopes or part thereof shown in Table 1 or an equivalent homologoussequence from the membrane proximal region of other receptors.

The antibody of the invention does not bind only to the C′-D loop ofCD28, and thus in one embodiment will bind both the C′-D loop of CD28and another region of CD28. The antibody may be one which does not bindany portion of the C′-D loop of CD28. The antibody may or may not bindto the C′-D loop (or the equivalent loop) of any other member of theCD28 family of proteins. The antibody of the invention may or may notbind to any or all of the sequences shown in Table 3.

The first type of chimeric protein of the invention may or may not bindto only the C′-D loop of CD28. The first type of chimeric protein may beone which does not bind to any portion of the C′-D loop of CD28. Thefirst type of chimeric protein may or may not bind to the C′-D loop (orthe equivalent loop) of any other member of the CD28 family of proteins.The first type of chimeric protein may or may not bind to any or all ofthe sequences shown in Table 3.

The second type of chimeric protein of the invention may or may not bindto only the C′-D loop of CD28. The second type of chimeric protein maybe one which does not bind to any portion of the C′-D loop of CD28. Thesecond type of chimeric protein may or may not bind to the C′-D loop (orthe equivalent loop) of any other member of the CD28 family of proteins.The second type of protein may or may not bind to any or all of thesequences shown in Table 3.

Receptors Bound by the Antibody and Chimeric Protein

The receptors which are bound by the antibody or chimeric protein of theinvention are expressed on the cell surface. The receptor is capable ofbeing phosphorylated (typically at one or more tyrosine residues in thecytoplasmic region of the receptor), and phosphorylation of the receptorwill typically lead to its activation. The receptor will comprise acytoplasmic domain that is dependent on extrinsic protein kinases to bephosphorylated. Thus the receptor will not have an intrinsic enzymatic(kinase or phophatase) activity. The receptor will typically comprisetyrosine-containing, activating ITAM motifs (YxxL/Ix₇₋₁₂YxxL/I),inhibitory ITIM motifs (I/V/L/SxYxxL/V) or “switch” (TxYxxV/I;activating and inhibitory) signalling motifs (where x is any aminoacid). These motifs are phosphorylated by cytoplasmic tyrosine kinases,such as the Src kinases, in competition with antagonistic tyrosinephosphatases, such as CD45. The signalling character of the receptors isdefined exclusively by the nature of these motifs (ITAM vs ITIM:activating vs inhibitory).

The receptor may be a member of any surface protein superfamily, but istypically a member of the immunoglobulin superfamily. The receptor maybe a member of the CD28 superfamily. The receptor may be any of thespecific receptors which are shown in Table 1 or 2 or may comprise asequence which is homologous to the sequence of any of these specificreceptors. The receptor may be CD28, CTLA-4, ICOS, PD-1 or BTLA orcomprise a sequence which is homologous to the sequence of any of thesespecific receptors.

The receptor may be of any of the species that are mentioned herein, andthus may be a mammalian or avian, preferably rodent (such as mouse orrat) or primate (such as human) receptor.

The receptor may be naturally present on a cell of the immune systemsuch as a I′ cell, B cell, myeloid cell, mast cell, NK cell or agranulocyte.

Screening Methods of the Invention

The invention provides a method of obtaining an agent which is capableof superagonising the above-mentioned receptor which binds to theantibody and chimeric protein of the invention. The method comprisesdetermining whether a candidate agent has any of the propertiesdiscussed above possessed by the antibody or chimeric protein of theinvention which allows them to induce superagonistic signalling by areceptor. Thus the method will generally comprise determining whether acandidate agent is able to bind to an extracellular membrane proximalregion of the receptor, and may also determine whether a candidate agentis able to bind

-   at a particular location from the cell membrane (such as within 75 Å    of the cell membrane),-   to particular sequences (such as the sequences mentioned in Table    1), or-   in a manner (such as orthogonal to the main axis of the bound    receptor domain)    which is discussed above for binding of the antibody or chimeric    protein of the invention.

Suitable extracellular membrane proximal regions of a receptor may beidentified using a sequence database search algorithm (e.g. BLAST) tosearch for the solved structure most related to the receptor in theprotein structural database (PDB). This structure would then be used toidentify membrane proximal regions (preferably □-strands) in thereceptor by sequence alignment. Whether or not a candidate agent bindsto the identified sequences could be determined by taking the midpointof the strand and the side chains of amino acids two- and three-residuesbelow this mid-point could then be mutated. Mutant forms of the receptormutated at each of these residues would then be expressed (e.g. onsuitable target cells that can be transfected stably or transiently,such as 293T cells), and used to screen for candidate agents that boundthe non-mutated receptor but failed to bind the mutant receptor, forexample using fluorescence-activated cell sorting. It is envisaged thatonly every second strand in a conventional beta barrel protein wouldneed to be mutated. In the case of a receptor which is a member of theimmunoglobulin superfamily, this corresponds to 8-10 mutant lines beinggenerated, in total. Examples of this process are shown in FIG. 2 forthe immediate CD28 family members (ICOS and CTLA-4) and for the PD-1protein, which is representative of receptors not related to CD28.

Agents that induce superagonistic signalling by a receptor may also beidentified using a peptide which comprises sequence from theextracellular membrane proximal region of a receptor or homologoussequence thereto. Such a sequence is typically 5 to 20 amino acids long,such as 10 to 15 amino acids long. In one embodiment the agent isidentified using peptide arrays. The mapping of discontinuous epitopescan be performed using overlapping peptides derived from the entireprimary sequence of a protein. The whole protein sequence is generatedin the form of short overlapping peptides (for example each shifted by 3amino acids), e.g. prepared by standard spot synthesis (Automated SpotSynthesiser, Abimed, Langenfeld, Germany) on Whatman 50 filter paper(Whatman, Maidstone, England). The C-termini of the peptides may beattached to cellulose via a (□-Ala)₂ spacer and the N-terminiacetylated. The arrays may be subsequently probed either directly on thepeptide membrane or after transfer to another surface (e.g.nitrocellulose) using the monoclonal antibody and achemiluminescence-based detection system (ECL Western Blotting DetectionSystem, Amersham Pharmacia Biotech).

Suitable candidate agents that can be tested in the above screeningmethods include antibody agents (for example, monoclonal and polyclonalantibodies, single chain antibodies, chimeric antibodies and CDR-graftedantibodies). Furthermore, combinatorial libraries, defined chemicalidentities, peptide and peptide mimetics, oligonucleotides and naturalagent libraries, such as display libraries (e.g. phage displaylibraries) may also be tested. The candidate agents may be chemicalcompounds, which are typically derived from synthesis around smallmolecules. Batches of the candidate agents may be used in an initialscreen of, for example, ten substances per assay, and the agents ofbatches which show the required property tested individually.

Obtaining a Superagonistic Antibody

The invention provides methods for obtaining a superagonistic antibody.In one embodiment the method comprises screening antibodies for theability to superagonise a receptor, wherein the antibodies have beenobtained either by immunizing an animal with the receptor or a homologueor fragment of the receptor, or from a combinatorial antibody library.Preferably the animal is immunized with a protein which comprises thesequences shown in Table 1, or a fragment thereof, or a homologue ofsuch a sequence or fragment.

Whether or not an antibody is a superagonistic antibody may beascertained by determining whether or not the antibody can bind anextracellular membrane proximal region of a receptor, for example usingany of the suitable methods described above in the section of screeningfor superagonistic agents.

Alternatively the antibody can be tested in an activity assay to seewhether or not it causes activation of the receptor. Receptors that areactivated by the antibody will either transduce an activating signal oran inhibitory signal to the cell cytoplasm. In the case of receptorswhich transduce an activating signal, as in the case of CD28, theactivity which is tested may be activation of the cell. This can bedetermined using functional screens based on, for example, cellproliferation, increased cellular calcium, enhanced tyrosinephosphorylation of proteins in the cell or the production of/release ofsubstances by the cell, e.g. IL-2 production. In the case of receptorsthat transduce inhibitory signals when activated, activation of suchreceptors can be ascertained by detection of any of the changes whichoccur to the receptor or the cell when such a signal is transduced, suchas changes in the extent of phosphorylation of one or more proteins inthe cell.

The binding or activity assay may be carried out in vitro (inside oroutside a cell) or in vivo.

In one embodiment of the method the antibody is obtained by immunisingan animal with a peptide comprising a sequence of length 5 to 20 aminoacids which represents an extracellular membrane proximal region of thereceptor, or a homologue of such sequence, and obtaining the antibodyproduced by the animal against said sequence. Alternatively the antibodymay be obtained by selecting an antibody from a combinatorial librarybased on its ability to bind a peptide comprising a sequence of length 5to 20 amino acids which represents an extracellular membrane proximalregion of the receptor, or a homologue of such sequence.

In one embodiment the peptide which is used to immunise the animal orselect an antibody from a library only comprises sequence from thereceptor, or a homologue thereof, and does not comprise any additionalsequence. In this embodiment the peptide will only be 5 to 20 aminoacids long. However in other embodiments the peptide comprises othersequence to the N-terminus and/or C-terminus of the receptor sequence,such as sequence which is different from (and typically not homologousto) receptor sequence.

Antibodies

Various types of antibodies are mentioned herein, including antibodiesobtained by immunisation. Such antibodies may be raised against specificepitopes/sequences. An antibody, or other compound, “specifically binds”to a sequence when it binds with preferential or high affinity to thesequence for which it is specific but does not bind or binds with onlylow affinity to other sequences. A variety of protocols for competitivebinding or immunoradiometric assays to determine the specific bindingcapability of an antibody are well known in the art (see for exampleMaddox et al, J. Exp. Med. 158, 1211-1226, 1993). Such immunoassaystypically involve the formation of complexes between the specificsequence and its antibody and the measurement of complex formation.

Means for preparing and characterising antibodies are well known in theart, see for example Harlow and Lane (1988) “Antibodies: A LaboratoryManual”, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.For example, an antibody may be produced by raising antibody in a hostanimal.

A method for producing a polyclonal antibody comprises immunising asuitable host animal, for example an experimental animal, with theimmunogen and isolating immunoglobulins from the animal's serum. Theanimal may therefore be inoculated with the immunogen, bloodsubsequently removed from the animal and the IgG fraction purified.

A method for producing a monoclonal antibody comprises immortalisingcells which produce the desired antibody. Hybridoma cells may beproduced by fusing spleen cells from an inoculated experimental animalwith tumour cells (Kohler and Milstein (1975) Nature 256, 495-497).

An immortalized cell producing the desired antibody may be selected by aconventional procedure. The hybridomas may be grown in culture orinjected intraperitoneally for formation of ascites fluid or into theblood stream of an allogenic host or immunocompromised host. Humanantibody may be prepared by in vitro immunisation of human lymphocytes,followed by transformation of the lymphocytes with Epstein-Barr virus.

For the production of both monoclonal and polyclonal antibodies, theexperimental animal is suitably a goat, rabbit, rat, mouse, guinea pig,chicken, sheep or horse. If desired, the immunogen may be administeredas a conjugate in which the immunogen is coupled, for example via a sidechain of one of the amino acid residues, to a suitable carrier. Thecarrier molecule is typically a physiologically acceptable carrier. Theantibody obtained may be isolated and, if desired, purified.

The Peptide of the Invention

The invention also provides a peptide of length 5 to 20 amino acidscomprising a sequence which binds to the superagonistic antibody of theinvention. The peptide may have a length of 10 to 15 amino acids. Thepeptide may comprise sequence from an extracellular membrane proximalregion of any of the receptors mentioned herein or have a homologoussequence thereto (such sequence may have a length of 5 to 20 aminoacids). The peptide may thus comprise any of the sequences shown inTable 1 or homologues of such sequences.

Therapeutic Aspects of the Invention

The invention provides substances which can be used to treat a patient.The patient is typically a human or animal, such as a mammal or bird,for example a cow, sheep, goat, pig, camel, horse, dog, cat, goose, duckor chicken.

The modulator of CD28 which is identified from a structural model orstructural coordinates of CD28 can be used to modulate the immune systemof a patient. Such modulators will either agonise or antagonize CD28(i.e. cause or contribute to an increase or decrease, respectively, inCD28 signalling activity). Modulators which agonise CD28 may be used tostimulate the activity of T cells (which express CD28) and thus tostimulate an immune response against an antigen. Such modulators maytherefore be used in the prevention or treatment of a disease caused bya pathogenic agent, such as a virus, microorganism (for example abacterium) or multicellular organism; or in the prevention or treatmentof a cancer.

Modulators which antagonise CD28 may be used to inhibit the activity ofT cells, and thus to inhibit an immune response to an antigen. Suchmodulators may be used to prevent or treat an autoimmune disease (suchas rheumatoid arthritis or asthma), allergy or transplantationrejection.

The superagonistic antibodies, chimeric proteins and agent describedherein may also be used in the therapy of patients to prevent or treat adisease. These substances may be used to modulate the state of a cell onwhich the relevant receptor is present. Thus the substance may activateor inhibit cell activity depending on whether the activated receptortransduces a stimulatory or inhibitory signal to the cytoplasm of thecell. The modulation of the cell may thus be used to treat a diseasecaused by the cell or a disease which can be alleviated or prevented bythe cell.

In a preferred embodiment the cell is a cell of the immune system (e.g.any such cell mentioned herein) and therefore superagonistic antibodies,chimeric proteins and agents may be used to modulate the immune responseof a patient. Superagonistic antibodies, chimeric proteins and agentswhich stimulate an immune response may be used in the prevention ortreatment of a disease caused by a pathogenic agent, such as a virus,microorganism (for example a bacterium) or multicellular organism; or inthe prevention or treatment of a cancer. Superagonistic antibodies,chimeric proteins and agents which inhibit an immune response may beused to prevent or treat an autoimmune disease (such as rheumatoidarthritis or asthma), allergy or transplantation rejection.

The patient may also be treated by generating a superagonistic antibodyresponse in the patient by immunisation with a peptide that stimulatesthe generation of such a response. The antibody that is generated willbe specific to a sequence present in the peptide. The peptide willcomprise sequence from the extracellular membrane proximal region of areceptor, or sequence which is homologous thereto. Such receptorsequence may be any of the membrane proximal sequences of a receptor (orhomologues thereof) mentioned herein. Thus the peptide may be any of thepeptides mentioned herein which comprise such sequence. In oneembodiment a nucleic acid capable of expressing any of theabove-mentioned therapeutic substances is administered to the patient.Such a nucleic acid typically comprises a region which encodes thetherapeutic substance and a control region which causes expression ofthe coding sequence, such as a promoter. Thus the nucleic acid may be inthe form of a vector.

The Substances Mentioned Herein

The modulators, antibodies, chimeric proteins, peptides and nucleicacids mentioned herein may be present in a substantially isolated form.They may be mixed with carriers or diluents which will not interferewith their intended use and still be regarded as substantially isolated.They may also be in a substantially purified form, in which case theywill generally comprise at least 90%, e.g. at least 95%, 98% or 99%, ofthe proteins nucleic acids or dry mass of the preparation.

Homologues

Homologues of protein sequences are referred to herein. Such homologuestypically have at least 50% homology, preferably at least 60%, 70%, 80,90%, 95%, 97% or 99% homology, for example over a region of at least 15,20, 30, 100 more contiguous amino acids. The homology may be calculatedon the basis of amino acid identity (sometimes referred to as “hardhomology”).

For example the UWGCG Package provides the BESTFIT program which can beused to calculate homology (for example used on its default settings)(Devereux et al (1984) Nucleic Acids Research 12, p 387-395). The PILEUPand BLAST algorithms can be used to calculate homology or line upsequences (such as identifying equivalent or corresponding sequences(typically on their default settings), for example as described inAltschul S. F. (1993) J Mol Evol 36:290-300; Altschul, S, F et al (1990)J Mol Biol 215:403-10.

Software for performing BLAST analyses is publicly available through theNational Center for Biotechnology Information(http://www.ncbi.nlm.nih.gov/). This algorithm involves firstidentifying high scoring sequence pair (HSPs) by identifying short wordsof length W in the query sequence that either match or satisfy somepositive-valued threshold score T when aligned with a word of the samelength in a database sequence. T is referred to as the neighborhood wordscore threshold (Altschul et al, supra). These initial neighborhood wordhits act as seeds for initiating searches to find HSPs containing them.The word hits are extended in both directions along each sequence for asfar as the cumulative alignment score can be increased. Extensions forthe word hits in each direction are halted when: the cumulativealignment score falls off by the quantity X from its maximum achievedvalue; the cumulative score goes to zero or below, due to theaccumulation of one or more negative-scoring residue alignments; or theend of either sequence is reached. The BLAST algorithm parameters W, Tand X determine the sensitivity and speed of the alignment. The BLASTprogram uses as defaults a word length (W) of 11, the BLOSUM62 scoringmatrix (see Henikoff and Henikoff (1992) Proc, Natl. Acad. Sci. USA 89:10915-10919) alignments (B) of 50, expectation (E) of 10, M=5, N=4, anda comparison of both strands.

The BLAST algorithm performs a statistical analysis of the similaritybetween two sequences; see e.g., Karlin and Altschul (1993) Proc. Natl.Acad. Sci. USA 90: 5873-5787. One measure of similarity provided by theBLAST algorithm is the smallest sum probability (P(N)), which providesan indication of the probability by which a match between two amino acidsequences would occur by chance. For example, a sequence is consideredsimilar to another sequence if the smallest sum probability incomparison of the first sequence to the second sequence is less thanabout 1, preferably less than about 0.1, more preferably less than about0.01, and most preferably less than about 0.001.

The homologous sequence typically differs by at least 1, 2, 5, 10, 20 ormore mutations (each of which may be a substitution, deletion orinsertion of an amino acid). These mutations may be measured across anyof the regions mentioned above in relation to calculating homology. Thesubstitutions are preferably conservative substitutions. These aredefined according to the following Table. Amino acids in the same blockin the second column and preferably in the same line in the third columnmay be substituted for each other:

ALIPHATIC Non-polar G A P I L V Polar - uncharged C S T M N Q Polar -charged D E K R AROMATIC H F W Y

Administration

The formulation of any of the therapeutic substances mentioned hereinwill depend upon factors such as the nature of the substance and thecondition to be treated. Any such substance may be administered in avariety of dosage forms. It may be administered orally (e.g. as tablets,troches, lozenges, aqueous or oily suspensions, dispersible powders orgranules), parenterally, subcutaneously, intravenously, intramuscularly,intrasternally, transdermally or by infusion techniques. The substancemay also be administered as suppositories. A physician will be able todetermine the required route of administration for each particularpatient.

Typically the substance is formulated for use with a pharmaceuticallyacceptable carrier or diluent. The pharmaceutical carrier or diluent maybe, for example, an isotonic solution. For example, solid oral forms maycontain, together with the active compound, diluents, e.g. lactose,dextrose, saccharose, cellulose, corn starch or potato starch;lubricants, e.g. silica, talc, stearic acid, magnesium or calciumstearate, and/or polyethylene glycols; binding agents; e.g. starches,arabic gums, gelatin, methylcellulose, carboxymethylcellulose orpolyvinyl pyrrolidone; disaggregating agents, e.g. starch, alginic acid,alginates or sodium starch glycolate; effervescing mixtures; dyestuffs;sweeteners; wetting agents, such as lecithin, polysorbates,laurylsulphates; and, in general, non-toxic and pharmacologicallyinactive substances used in pharmaceutical formulations. Suchpharmaceutical preparations may be manufactured in a known manner, forexample, by means of mixing, granulating, tabletting, sugar-coating, orfilm coating processes.

Liquid dispersions for oral administration may be syrups, emulsions andsuspensions. The syrups may contain as carriers, for example, saccharoseor saccharose with glycerine and/or mannitol and/or sorbitol.

Suspensions and emulsions may contain as carrier, for example a naturalgum, agar, sodium alginate, pectin, methylcellulose,carboxymethylcellulose, or polyvinyl alcohol. The suspensions orsolutions for intramuscular injections may contain, together with theactive compound, a pharmaceutically acceptable carrier, e.g. sterilewater, olive oil, ethyl oleate, glycols, e.g. propylene glycol, and ifdesired, a suitable amount of lidocaine hydrochloride.

Solutions for intravenous or infusions may contain as carrier, forexample, sterile water or preferably they may be in the form of sterile,aqueous, isotonic saline solutions.

A therapeutically effective amount of substance is administered. Thedose may be determined according to various parameters, especiallyaccording to the substance used; the age, weight and condition of thepatient to be treated; the route of administration; and the requiredregimen. Again, a physician will be able to determine the required routeof administration and dosage for any particular patient. A typical dailydose is from about 0.1 to 50 mg per kg, preferably from about 1.0 mg perkg to 10 mg per kg of body weight, according to the activity of thespecific therapeutic substance, the age, weight and condition of thesubject to be treated, the type and severity of the disease and thefrequency and route of administration. Preferably, daily dosage levelsare from 5 mg to 2 g.

The following Examples illustrate the invention:

EXAMPLES Overview of Crystallisation Strategy

In order to undertake crystallization trials with glycoproteins, such asthe CD28 homodimer, it is generally necessary to produce large amountsof protein (>10 milligrams). For this the glutamine synthetase-basedChinese hamster ovary cell expression system (Lonza Biologics Plc, UK)was chosen, which is one of the few eukaryotic expression systemscapable of glycoprotein production at this level. The Lec3.2.8.1 cellline was used as the expression host as this provides the option ofenzymatically removing unnecessary glycosylation after protein foldingand secretion has taken place, which generally favours crystallization.In order to enhance homodimerization, the protein was expressed in theform of a fusion protein with the Fc region of immunoglobulin, which isitself a homodimer. Because the link between the CD28 portion and the Fcis extremely flexible (which will generally discourage crystallization),the construct was prepared in a way that made it thrombin-cleavable.

It was found that producing active, thrombin-cleavable protein dependedon the location of the cleavage site. When the cleavage site was tooclose to the ligand binding domain (to make a more compact protein forcrystallization), the protein produced was mis-folded.

In general removal of the N-linked glycans from a glycoproteinsubstantially enhances its ability to crystallise. Unexpectedly, and inmarked contrast to other cell surface glycoproteins, which are generallyvery stable and active after deglycosylation, the CD28 homodimer provedto be very unstable after deglycosylation with the enzymeendoglycosidase H at the slightly acidic pH at which this enzyme isactive. Therefore it was necessary to leave the glycosylation of CD28intact. The glycosylated form of the homodimer failed to crystallise inmore than 100 trials.

To reduce the impact of the glycosylation, therefore, an alternativestrategy was employed, in which Fab fragments of anti-CD28 antibodieswere prepared and crystallised with CD28. Fab fragments are almostinvariably unglycosylated, so the formation of complexes with the Fabcan be expected to reduce the impact of the glycans by 80%, regardlessof where they bind the protein of interest (a Fab is four times the sizeof the CD28 monomer). The likelihood that the complex of the Fab andprotein of interest will crystallise depends on the overall shape of thecomplex, (i.e. its “compactness”) and on the chemical properties of thesurfaces of the complex involved in forming crystal lattice contacts.Because the surface of the complex that will form lattice contactscannot be predicted a priori, it is impossible to predict whether agiven Fab will give crystals when complexed with the protein. Notunexpectedly, therefore, the homodimer still failed to crystallize afterbeing complexed with one Fab molecule per homodimer for two differentantibodies (5.11A1 and 9D7), or two Fabs per homodimer for one of theseantibodies (5.11A1), in >100 trials per complex.

In addition the stalk region was expected to create problems. The key toobtaining crystals was to prepare monomeric CD28 by gentle reduction andalkylation of the interchain disulphide bond in the stalk-like region ofthe homodimer. The expectation was that the presence of an intactdisulphide in the stalk might rigidify the stalk, making the overallstructure less compact. Two complexes formed with the monomer and twodifferent Fabs (5.11A1 and 9D7) gave crystals, but only one of thesecrystal forms (obtained with 5.11A1 Fab fragments) proved to be suitablefor data collection. Other antibodies may also have been useful incrystallising CD28, including antibodies that bind to the same loop ofCD28 as bound by 5.11A1 (the C′-D loop) or that bind to the ligandbinding site of CD28. Suitable antibodies are described inUS-A1-2003/0166860 and Luhder et al (2003) J. Exp. Med., 197, 955-66.

Preparation of CD28 Homodimer (i) Construct Generation

The polymerase chain reaction (PCR) was used to amplify complementaryDNA (cDNA) encoding the signal peptide sequence of mouse immunoglobulinheavy chain and the extracellular domain of CD28 (residues 1-134 of themature polypeptide; SEQ ID NO:1) from 44 ng of the plasmid, pKGe 1145.The oligonucleotides used in the reaction added, at the 5′ end, an Xba Icleavage site and 24 nt of the rat CD4 5′ untranslated region and, atthe 3′ end, codons for the thrombin cleavage site, LVPRGS. The sequencesof the oligonucleotide primers used for this were (oligonucleotidesequences are given in 5′ to 3′ direction) CD28T_(—)5′: TAG TAG TCT AGACCC CAT CCG CTC AAG CAG GCC ACC ATG GAT TGG CTG CGG AAC TTG (SEQ ID NO:11); and CD28T_(—)3′: CTA CCA CTA CCC CTG GGT ACC AGG GGC TTA G (SEQ IDNO: 12). In a second reaction, cDNA encoding the heavy chain constant(C) region-2 and C region-3 domains of murine Ig (residues 103-323 ofthe secreted protein) was amplified by PCR from 44 ng of pKGe1145. Atthe 3′ end, the oligonucleotides added an Xba I restriction site to aidcloning. The NH2-terminus of the protein encoded by the Ig cDNA had thesequence GSKPSIS rather than GCKPCIC. The sequences of theoligonucleotide primers for this reaction were Ig_(—)5′: CTA AGC CCC TGGTAC CCA GGG GTA GTG GTA G (SEQ ID NO: 13); and Ig_(—)3′: CTA CTA TCT AGATTA TTT ACC AGG AGA GTG GGA G (SEQ ID NO: 14). The PCR conditions forboth reactions were: denaturation at 94° C. for 15 s, annealing at 59°C. for 30 s, extension at 68° C. for 210 s; 2.5U of Accuzyme polymerase(Bioline Ltd, U.K.), 300 nM oligonucleotides and 200 μM dNTPs were usedand the reactions run for 20 cycles.

In a third reaction, PCR was used to anneal the two initial productstogether to generate cDNA encoding a chimera consisting of the CD28extracellular domain fused to murine IgGFc via a thrombin cleavablesequence (called the CD28TFc chimera; SEQ ID NO:2). Ten microlitres ofeach of the initial PCR reaction product mixtures was used as templateand 300 nM of the CD28_(—)5′ and Ig_(—)3′ oligonucleotides was alsoadded to the mix. The PCR conditions were otherwise identical to thefirst set except that they were run for only 4 cycles. The PCR productwas gel-purified, cut with Xba I and cloned into the Xba I site of pEE14for expression in the glutamine synthetase-based gene expression system(Lonza Biologics Plc, UK). The plasmid construct was then sequencedusing dideoxy sequencing.

(ii) Expression of the CD28TFc Chimera

Lec3.2.8.1 cells, 2×10⁶/flask, were transfected with 20 μg of DNA/flaskfor 3 h using Pfx-8 lipids (Invitrogen Inc), and then the cells werecloned the following day by plating out at 2×10⁶ cells/96-well plate.After 2 weeks, the clones expressing the highest amounts of CD28TFc wereselected by Western-blotting using the semi-quantitive ECL detectionsystem (Amersham-Pharmacia, UK) and an anti-mouse Fc primary antibody(Sigma-Aldrich Co.). The best-expressing clone was expanded and grown toconfluence in bulk culture in Cell Factories (Nunc A/S, Denmark)containing immunoglobulin-free medium (Invitrogen-Gibco Ltd, UK) in thepresence of 2 mM sodium butyrate. The supernatant was harvested afterapproximately four weeks and clarified by centrifugation at 5,000 g.

(iii) CD28TFc Purification

The spent, clarified medium was concentrated to approximately ⅙ of theoriginal volume using a Masterflex L/S concentrator (HelixxTechnologies, Inc.). The sample was then buffer-exchanged against theoriginal volume of 3M NaCl, 1.5M glycine, pH 8.5 high-salt buffer andthe protein incubated with Protein A Sepharose beads (Sigma-Aldrich Co.)using 10 mls of swollen beads/litre of concentrated supernatant at 1litre of supernatant/5 litre conical flask or beaker. The pH wasadjusted to pH 8.5 with 2.75 M Tris, pH 8.5 to allow binding of theCD28TFc to Protein A overnight with gentle stirring at 4° C.

The beads were allowed to settle for 2 h prior to siphoning off thedepleted supernatant. The settled beads were then transferred to 50 mlFalcon tubes (Becton Dickinson Biosciences, UK) and recovered bycentrifugation at 200 g. The beads were transferred to a 30 ml column(Biorad, UK) and then washed with 300 mls of cold phosphate-bufferedsaline, 0.5 M NaCl, pH 8.3. The column was eluted with 0.1 M citricacid, pH 3.0 into 10×2 ml fractions in glass tubes containing 0.4 ml of2.75M Tris, pH 8.5 for immediate neutralization of the citric acid.Fractions containing CD28TFc according to analysis by 12% sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) were concentratedto 0.5 ml or no higher concentration than 20 mg/ml using a Centriprep 10concentrator (Millipore Corp). The protein was then applied to aSuperdex 200 H/R gel filtration column (Amersham Biosciences)pre-equilibrated with 10 mM Hepes, 140 mM NaCl, pH 7.4 (HBS buffer) forup to three successive runs. Eluting fractions were monitored forabsorbance at 280 nm. Protein-containing fractions were examined bySDS-PAGE. Each cycle of batch purification yielded ˜9 mgs of CD28TFc; upto 7 batch-purifications was required to deplete all the CD28TFc per setof 6 cell factories (˜5 litres of starting tissue culture supernatant).

(iv) Thrombin Protease Cleavage of CD28TFc

The most-pure fractions containing CD28TFc were pooled for thrombincleavage.

Initial trial titrations dictated the use of the following conditionsfor large-scale cleavage of CD2TFc: lyophilized thrombin (Sigma-AldrichCo.) was re-hydrated in HBS buffer to a concentration of 1U/μl and thiswas then added to the CD28TFc at 0.11 μl/6 μg of protein for overnightdigestion at 37° C. The reaction was stopped by addition of freshlyre-hydrated benzamidine to a final concentration of 1 mM. A 12%non-reducing SDS-PAGE gel was used to confirm the extent of cleavage.

(v) Purification of the CD28 Homodimer

The pH of the thrombin-cleaved protein was adjusted to pH 8.5 using2.75M Tris pH 8.5, prior to concentration to 0.5 ml using a Centriprep10 concentrator (Millipore Corp). Fresh Protein A beads were washed andrehydrated to a final volume of ˜5 mls, prior to being packed into a 0.7cm×20 cm Econo-column (Bio-Rad, U.K.) and then equilibriated with HBS,pH 8.5 at 4° C. The concentrated protein was then applied to the column,allowed to run into the bed, and then sequential fractions were elutedby addition of 0.5 ml of HBS, pH 8.5 to the top of the bed every 10minutes for 2 h. The absorbance of each fraction was determined at 280nm. The extent of separation of the Fc from the thrombin-released CD28homodimer was determined by 12% SDS-PAGE analysis of the fractions undernon-reducing conditions. The critical steps for good separation were (1)to allow the protein to pass slowly through the column and (2) toconduct the separation at 4° C. The homodimer was concentrated to 0.5 mland subjected to gel-filtration on a Superdex 75 H/R column (AmershamBiosciences). The purified homodimer was used for crystallizationtrials, reduced and alkylated for other crystallization trials (seebelow), or frozen at −80° C. for future use.

Preparation of Fab Fragments of 5.11A1 Antibody

Fab fragments were prepared using the Pierce Biotechnology ImmunoPure®Fab Preparation Kit, as briefly outlined below.

(i) Fab Fragment Generation and Purification

Nine millilitres of whole, purified 5.11A1 antibody at 0.3 mg/ml in PBSwas concentrated to 1 ml and then diluted to 10 mls with 20 mM sodiumphosphate, 10 mM EDTA, pH 7 and then re-concentrated to 0.5 ml. To thiswas added 0.5 ml of 20 mM sodium phosphate, pH 7 containing 3.5 mg/mlcysteine.HCl. The 1 ml mixture was then added to 0.5 ml of a 50% slurryof Sepharose-immobilized Papain supplied with the kit, which had beenpre-equilibrated with 20 mM sodium phosphate pH 7 containing 3.5 mg/mlcysteine.HCl. This was then incubated for 5 hours in a shaking waterbath at 37° C. The cleaved Fab and Fe fragments and undigested IgG wereseparated from the Immobilized Papain beads by centrifugation at 1000 gand the beads rinsed with 1.5 ml of the ImmunoPure IgG Binding Buffersupplied with the kit. The wash was then combined with the crude digestand the mix applied to a Sepharose-immobilized Protein A columnpre-equilibrated with 13 ml of ImmunoPure IgG Binding Buffer. The columnwas washed with 6.0 ml of the Binding Buffer and the eluate containingthe Fab fragments collected (9 mls in total). The eluate wasconcentrated to 0.5 ml and applied to a Superdex 200 H/R gel filtrationcolumn (Amersham Biosciences) pre-equilibrated with HBS buffer. Elutingfractions were monitored for absorbance at 280 nm. Protein-containingfractions were examined by SDS-PAGE.

Preparation of a Crystallizable form of CD28

(i) Reduction and Alkylation of CD28

CD28 has an interchain disulphide bond within the “stalk-like” regionthat is largely responsible for homodimerization. It was speculated thatits location within the stalk would render it more sensitive to reducingagents than the “canonical” disulphide bond and one other disulphideburied within the ligand-binding domain. If true this meant thatmonomeric CD28 could be generated which was native-like and fully activefor ligand and antibody binding. The minimum concentration of reducingagent required to release the monomer was ascertained by titrating thereducing agent, dithiothreitol (DTT). The reduced cysteines in theprotein and excess DTT were each then inactivated by alkylation with a2.2-fold molar excess of iodoacetamide (IAA). This titration indicatedthat the optimal conditions were 12.5 μM protein, 1.5 mM DTT and 3.3 mMIAA. The reduced protein sample, generally ˜4 ml, was finallyconcentrated to 0.5 ml and separated from unreduced homodimer bygel-filtration on a Superdex 75 H/R column (Amersham Biosciences).

Crystallographic Methods (i) Crystallization and Data Collection

The CD28/5.11A1 Fab complex was formed by incubating a 2:1 molar ratioof CD28 and Fab, followed by concentration of the protein mixture to15.6 mg/ml in HBS (the extinction coefficient, ε, of CD28 is 1.7 andthat of the Fab is 1.4). Crystals were grown at 18° C. in 0.2 μl hangingdrops (0.1 μl of the protein mixture plus 0.1 μl of precipitatingreagent) set up using a Cartesian Robot (APS Robotics & Integration,llc; Brown et al (2003) J. Appl. Cryst. 36, 315-18). The precipitatingreagent consisted of a mixture of 0.2 M magnesium formate and 20%polyethylene glycol 3350 in water. Crystals appeared in 5-7 days andwere cooled to 100K in the precipitating reagent with glycerol added toa final concentration of 10%, using a Cryojet liquid nitrogen system fordata collection (Oxford Instruments, Abingdon, UK). Crystals belong tospace group C2 with unit cell dimensions a=191.2, b=47.4, c=71.8 Å and acalculated solvent content of 56-58% for one CD28 monomer and one Fabmolecule per asymmetric unit (ignoring the effects of glycosylation).The 2.7 Å resolution data were collected from a single crystal at 100Kat Beamline ID2 at the European Synchrotron Radiation Facility using aFReLoN CCD. One hundred and fifty-eight 1° rotation images werecollected and reduced with HKL2000 (Otwinowski and Minor (1997) MethodsEnzym. 276, 307-26).

(ii) Structure Determination and Refinement

The structure was solved by molecular replacement in XPLOR v3.851(Brunger (1992) X-PLOR Version 3.1. A system for X-ray crystallographyand NMR. Yale University Press, New Haven, Conn.) using a set of searchmodels generated from a single Fab structure (PDB accession no. 15c8) byvarying the elbow angle in 2° steps using an XPLOR script. A singleunambiguous solution was found when the elbow angle was altered by −8°as defined by the script. After rigid body refinement of each domain ofthe Fab in CNS (Briinger et al. (1998) Acta Cryst. D54, 905-21), theR-factor was 44.4%. Initial electron density maps phased with Fab aloneshowed very limited, if any, electron density for the CD28 molecule. TheFab model was mutated to the sequence of 5.1 1A1 before proceeding withtwo cycles of manual rebuilding in O (Jones et al (1990) O: Amacromolecular modelling environment. Crystallographic and modelingmethods in molecular design. Bugg and Ealick, Eds. Springer-Verlag Press189-95) and positional refinement in CNS, reducing the R-factor to36.4%. A molecular replacement solution for CD28 was then found by usingonly regions of human CTLA-4 (from PDB 1i81) with strong sequencehomology to CD28, i.e. all the β-strands (except C′) and the EF and FGloops, as a search model and by fixing the refined Fab structure. Inorder to avoid model bias, the refined constant regions of the Fab werereplaced with the unaltered constant domains from PDB 15c8 bysuperposition using SHP (Stuart et al (1979) J. Mol. Biol. 134, 109-42),and the positions were fixed during refinement. The variable regions ofthe Fab and the CD28 monomer were refined in X-PLOR v3.851 usingpositional refinement followed by grouped and restrained individual Bfactor refinement along with manual rebuilding in O. All refinementprocedures used data from 25.0-2.7 Å and excluded 5% of reflectionsrandomly selected for generation of the R_(free) data set. Theanisotropy of the data was corrected for using X-PLOR and the data weresharpened for all but the last round of refinement. The current modelhas an R factor of 25.3% and an R_(free) of 33.4% against all data to2.7 Å. The model includes the CD28 monomer (excluding its C-terminalstalk) from residues 2 to 118, excluding residues Leu28 and Phe29, whichare poorly ordered, and the Fab, but with none of the sugar chains builtonto the CD28. The model has 76% of residues in the most favouredregions of the Ramachandran plot, with a further 21% in additionallyallowed regions.

The Structure of a CD28-Superagonist Antibody Complex

The ligand-binding V-set IgSF domain of the CD28 monomer, solved incomplex with 5.11A1 Fab, is very similar to the equivalent region ofCTLA-4. Lattice contacts generate a plausible CD28 homodimer. Whilstthis dimer is similar to the CTLA-4 homodimer (which is bivalent), thearrangement of the monomers in CD28 is sufficiently distinct to suggestan explanation for the apparent monovalence of CD28, i.e. the membraneproximal domains of ligands competing for each ligand binding site arelikely to clash. Cryoelectron microscopic analyses show that bothclasses of antibodies are bivalent, ruling out aggregation-basedexplanations for the differential signalling effects of conventionalversus superagonistic antibodies. The 5.11A1 Fab fragment bindsorthogonally to the surface formed by the CC′C″ strands of CD28,favouring the C′C″ “edge” of the monomer. This places the two long axesof the Fabs parallel to each other and the cell surface. In contrast,the epitopes of the conventional antibodies are close to the ligandbinding “top” of the homodimer.

Signalling by Receptors Dependent on Extrinsic Kinases

The existence of two classes of activating anti-CD28 antibodies, i.e.costimulatory antibodies and superagonists, greatly simplifies theanalysis of signalling by this archetypal costimulatory receptor. Theobservation that the two antibody classes have homologous effects in twodifferent species, and that these effects are predictable simplyaccording to the location of their epitopes, rules out trivialexplanations for the distinct signalling properties of these antibodies,e.g. affinity differences. Instead, the receptor-triggering problem isreduced to a comparison of the structural properties of the complexesthat these two types of antibodies form with CD28.

From the present crystal structure of the complex of CD28 with asuperagonistic antibody, it can be deduced that an antibody-inducedincrease in the net phosphorylation of the cytoplasmic domain of a givencell surface receptor, due to changes in the rates of phosphorylationand dephosphorylation of it, results in the triggering of that receptor(see FIG. 1). In more detail, the antibodies hold the cell surface atcertain distances from an immobilizing substrate (i.e. other, Fereceptor-bearing cells, in vivo or plastic, in vitro), such that themembrane separation in the region of the immobilized antibody andreceptor will differentially exclude, from the immediate vicinity of thereceptor, other molecules whose extracellular domains are comparable insize or larger than CD28-antibody complexes, such as the tyrosinephosphatase, CD45. In contrast, tyrosine kinases, e.g. p56lck, will beunaffected because they are small and/or attached to the inner leafletof the membrane. The result is that, overall, the phosphorylation ofCD28 will be favoured over its de-phoshorylation (by CD45), with the netincrease in phosphorylation amounting to receptor triggering.Superagonists are more potent than conventional antibodies because theybind epitopes close to the membrane rather than at the “top” of themolecule, leading to more efficient segregation of, e.g. CD45, andtherefore a larger increase in the net phosphorylation of CD28.

The important corollary of this explanation is that antibodies that bindthe membrane-proximal epitopes of the large number of other cell surfacemolecules not belonging to the CD28 subset of the immunoglobulinsuperfamily, but sharing the key signalling property of CD28-subsetmolecules, i.e. a reliance on extrinsic tyrosine kinases, for examplePD-1 and BTLA, should also potently invoke the activating or inhibitorysignalling properties of those molecules in precisely the same way,allowing the manipulation of cell behaviour with such antibodies.

PD-1 and BTLA

Programmed Death (PD)-1 protein modulates the responses of previouslyactivated T- and B-cells in secondary lymphoid organs or peripheral(parenchymal) tissues. PD-1 is expressed on a subset of thymocytes andupregulated on activated T, B and myeloid cells. The PD-1 ligands, B7-H1and B7-DC, are more widely expressed than those of CD28 family membersand are induced by pro-inflammatory agents on monocytes and dendriticcells and on activated but not resting B cells. B7-DC is expressed in avariety of peripheral tissues, including heart, pancreas, lung andliver. The B and T lymphocyte attenuator (BTLA), is also expressed onactivated T and B cells, but attenuates production of IL-2 after bindinga different peripherally expressed B7-related protein, called B7x.

PD-1 and BTLA are structurally comparable to other members of the CD28family only insofar as the proteins also consist of a single V-set IgSFdomain supported on stalks, and the cytoplasmic domains containimmunoreceptor tyrosine-based motifs (inhibitory in these two cases).The sequences of the IgSF domains of PD-1 and BTLA indicate that theseproteins are unrelated and do not belong to the same subset of the IgSFas CD28. Consistent with the view that PD-1 is involved in maintainingtolerance in normal T cells in the periphery, mice lacking the genedevelop a variety of autoimmune-like diseases dependent on the geneticbackground, including lupus-like arthritis and glomerulonephritis, andhave increased serum IgG3 and augmented B-cell proliferative responsesto anti-IgM in vivo.

Activating signalling by PD-1 and BTLA, because these molecules areinhibitory, would be expected to dampen a variety of peripheral immunepathologies. Signalling of this type could be stimulated by the bindingof superagonistic antibodies to the membrane proximal regions of theseproteins. A set of additional molecules, each of which has inhibitoryimmunoreceptor tyrosine-based signalling motifs and are thereforetargets for generating superagonists, is shown in Table 2.

Identifying Epitopes for Superagonists

The procedure is as follows. The “lower”, membrane-proximal half of themembrane proximal domain of a given structure, e.g. CD28 in FIG. 2A, isidentified by visual inspection of the structure. Superagonistic agentsbind residues in this region. For identifying superagonistic agents inreceptors closely related to the known structure, the equivalent regionsare identified in an alignment of the sequence of the known structurewith that of the related proteins as shown in FIG. 2A. To identify theepitopes of superagonistic antibodies in other molecules that have therequisite signalling properties but for which there is no structure,e.g. PD-1 in FIG. 2B, a structural model is identified by alignment ofthe sequence with that of the most similar molecule of known structurein the Protein Data Bank (in the example in FIG. 2B, the variable domainof the Ig kappa chain is the structure with the most similar sequence toPD-1). The “lower”, membrane proximal half of this domain is highlightedon the structure by visual inspection and the equivalent regions areidentified in the sequence alignment. To screen for superagonists, thesecond and third residues “down” (i.e. toward the membrane) in themarked epitopes shown in parts A and B are identified as shown in FIGS.2C and 2D. These residues, singly or together preferably form at leastpart of the epitopes of antibodies or other superagonistic agents, asbinding to these residues will require that the binding of the agent isparallel with the cell membrane. These residues can therefore be mutatedin proteins used for screening the superagonistic agents (i.e. agentsbinding to the unmutated protein but not the mutated protein, should besuperagonistic).

TABLE 1 Protein B C-C′ C″-D E F G CD28 family superagonistic epitopesEpitopes are named according to the strands from which they derive. A′hCD28 SPMLV AVNLS SLHKGLDSAVEVCV VYSKTGFNCDG FYLQN TDIYFC NGTIIHVhCTLA-4 PAVVL GIASFV TVLRQADSQVTEVCA FLDDSICTG LTIQG TGLYIC NGTQIYVhICOS YEMFI GVQIL QLLKGGQILCD VSIKSLKFCHS FFLYN ANYYFC TGGYLHI (SEQ ID(SEQ ID (SEQ ID (SEQ ID (SEQ ID (SEQ ID (SEQ ID NOs: 23, NOs: 26,NOs: 29, 30, NOs: 32, NOs: 35, NOs: 38, NOs: 41, 24, 25) 27, 28) 31)33, 34) 36, 37) 39, 40) 42, 43) PD-1 and BTLA superagonistic epitopes AhPD-1 PALLVV DNATF RMSPSNQTDK QPGQDCRFR MSVVR NDSGTY LRAELR hBTLA QSEHSIDPFEL KLNG QTSWK LHFEP NDNGSY TTLYVT (SEQ ID (SEQ ID (SEQ ID (SEQ ID(SEQ ID (SEQ ID (SEQ ID NOs: 44, NOs: 46, NOs: 48, 49) NOs: 50, NOs: 52,NOs: 54, NOs: 56, 45) 47) 51) 53) 55) 57)

TABLE 3 Protein Sequence hCD28 GNYSQQLQVYSKTGF (SEQ ID NO: 58) hCTLA-4YMMGNELTFLDDS (SEQ ID NO: 59) hICOS KTKGSGNTVSIKSLK (SEQ ID NO: 60)hPD-1 LAAFPEDRSQPGQDCR (SEQ ID NO: 61)

TABLE 2 Targets of inhibitory superagonist antibodies ReceptorChromosome Expression no. of ITIMs FcRIIB 1q23-24 B, myeloid, mast 1PILR 7q22 Myeloid 2 CD72 9p B 2 CD5 11q13 T, subset B 1 MAFA 12p12-13Myeloid, mast, NK 1 NKG2A 12p13.1-13.2 NK, T 2 CD31 17q23 Myeloid, etc 1CMRF35H 17q24 Leukocytes 3 CD22 19q13.1 B 4 CD66a 19q13.2 Granulocytes,etc 2 CD66d 19q13.2 Granulocytes 1 CD33 19q13.3 Myeloid 1 SIGLEC519q13.3 Myeloid 1 SIGLEC6 19q13.3 B cells, myeloid 1 SIGLEC7 19q13.3 NK,myeloid, etc 1 ILT2, 3, 4, 5 19q13.4 Myeloid, B, etc 4 LIR8 19q13.4Myeloid, B, etc 4 LAIR-1 19q13.4 Leukocytes 2 KIR2DL 19q13.4 NK, T 2KIR3DL 19q13.4 NK, T 2 SIRP 20p13 Myeloid, etc 2

TABLE 4 Coordinates of the CD28/5.11A1 crystal structure REMARKFILENAME=“/mnt/safe6/ed/cd28/cd28_all/xplor/ref18/positionaln3.pdb”REMARK  R= 0.251035 from 25 to 2.7 REMARK DATE:09-Jan-04  09:48:19    created by user: robert ATOM 1 CB ASP 1 122.28049.781 38.327 1.00 64.18 L ATOM 2 CG ASP 1 123.319 49.071 37.463 1.0067.78 L ATOM 3 OD1 ASP 1 122.934 48.417 36.470 1.00 66.82 L ATOM 4 OD2ASP 1 124.524 49.162 37.779 1.00 54.04 L ATOM 5 C ASP 1 122.104 52.26538.005 1.00 50.51 L ATOM 6 O ASP 1 122.541 53.057 37.163 1.00 41.52 LATOM 7 N ASP 1 121.674 50.711 36.112 1.00 37.53 L ATOM 8 CA ASP 1121.547 50.905 37.579 1.00 50.29 L ATOM 9 N ILE 2 122.073 52.527 39.3141.00 51.24 L ATOM 10 CA ILE 2 122.567 53.783 39.890 1.00 50.84 L ATOM 11CB ILE 2 121.842 54.094 41.240 1.00 22.62 L ATOM 12 CG2 ILE 2 122.27855.454 41.781 1.00 18.55 L ATOM 13 CG1 ILE 2 120.327 54.081 41.022 1.0030.61 L ATOM 14 CD1 ILE 2 119.510 54.521 42.229 1.00 31.86 L ATOM 15 CILE 2 124.086 53.714 40.119 1.00 54.91 L ATOM 16 O ILE 2 124.645 52.63040.283 1.00 55.68 L ATOM 17 N GLN 3 124.749 54.869 40.136 1.00 58.93 LATOM 18 CA GLN 3 126.204 54.923 40.322 1.00 56.95 L ATOM 19 CB GLN 3126.847 55.679 39.145 1.00 60.02 L ATOM 20 CG GLN 3 127.010 54.85137.863 1.00 63.51 L ATOM 21 CD GLN 3 126.479 55.552 36.615 1.00 65.36 LATOM 22 OE1 GLN 3 127.024 56.568 36.174 1.00 62.07 L ATOM 23 NE2 GLN 3125.412 55.003 36.038 1.00 72.37 L ATOM 24 C GLN 3 126.635 55.564 41.6501.00 51.69 L ATOM 25 O GLN 3 126.179 56.649 42.004 1.00 54.63 L ATOM 26N MET 4 127.522 54.889 42.376 1.00 42.42 L ATOM 27 CA MET 4 128.00555.392 43.660 1.00 37.64 L ATOM 28 CB MET 4 128.066 54.248 44.671 1.0054.55 L ATOM 29 CG MET 4 127.037 54.319 45.783 1.00 56.74 L ATOM 30 SDMET 4 125.360 54.633 45.189 1.00 76.06 L ATOM 31 CE MET 4 125.083 56.26945.843 1.00 68.01 L ATOM 32 C MET 4 129.392 56.008 43.507 1.00 44.16 LATOM 33 O MET 4 130.358 55.311 43.176 1.00 35.72 L ATOM 34 N ASN 5129.496 57.310 43.750 1.00 50.40 L ATOM 35 CA ASN 5 130.779 57.99643.624 1.00 58.90 L ATOM 36 CB ASN 5 130.703 59.060 42.521 1.00 67.94 LATOM 37 CG ASN 5 130.113 58.517 41.233 1.00 59.91 L ATOM 38 OD1 ASN 5130.783 57.811 40.474 1.00 49.08 L ATOM 39 ND2 ASN 5 128.848 58.83940.981 1.00 56.43 L ATOM 40 C ASN 5 131.191 58.643 44.939 1.00 46.55 LATOM 41 O ASN 5 130.612 59.637 45.368 1.00 40.94 L ATOM 42 N GLN 6132.202 58.076 45.577 1.00 33.77 L ATOM 43 CA GLN 6 132.661 58.60746.846 1.00 36.85 L ATOM 44 CB GLN 6 133.257 57.476 47.679 1.00 24.74 LATOM 45 CG GLN 6 132.395 56.218 47.684 1.00 19.84 L ATOM 46 CD GLN 6132.393 55.524 49.029 1.00 27.56 L ATOM 47 OE1 GLN 6 132.925 56.04850.013 1.00 30.84 L ATOM 48 NE2 GLN 6 131.803 54.339 49.082 1.00 34.41 LATOM 49 C GLN 6 133.677 59.725 46.647 1.00 43.10 L ATOM 50 O GLN 6134.328 59.800 45.608 1.00 55.89 L ATOM 51 N SER 7 133.807 60.598 47.6411.00 47.45 L ATOM 52 CA SER 7 134.736 61.714 47.543 1.00 54.98 L ATOM 53CB SER 7 134.285 62.858 48.469 1.00 70.71 L ATOM 54 OG SER 7 135.23263.917 48.514 1.00 61.37 L ATOM 55 C SER 7 136.187 61.304 47.812 1.0057.75 L ATOM 56 O SER 7 136.828 60.732 46.928 1.00 68.97 L ATOM 57 N PRO8 136.722 61.540 49.029 1.00 48.65 L ATOM 58 CD PRO 8 136.250 62.11650.300 1.00 21.82 L ATOM 59 CA PRO 8 138.118 61.109 49.140 1.00 47.63 LATOM 60 CB PRO 8 138.515 61.533 50.559 1.00 29.91 L ATOM 61 CG PRO 8137.525 62.576 50.933 1.00 26.41 L ATOM 62 C PRO 8 138.358 59.622 48.8931.00 48.77 L ATOM 63 O PRO 8 137.820 58.772 49.604 1.00 63.86 L ATOM 64N SER 9 139.161 59.318 47.876 1.00 33.02 L ATOM 65 CA SER 9 139.50057.939 47.548 1.00 32.48 L ATOM 66 CB SER 9 140.151 57.864 46.169 1.0030.85 L ATOM 67 OG SER 9 141.554 57.812 46.286 1.00 25.75 L ATOM 68 CSER 9 140.469 57.407 48.602 1.00 30.90 L ATOM 69 O SER 9 140.661 56.19748.737 1.00 32.96 L ATOM 70 N SER 10 141.076 58.333 49.340 1.00 29.68 LATOM 71 CA SER 10 142.022 58.018 50.412 1.00 35.34 L ATOM 72 CB SER 10143.387 57.645 49.831 1.00 49.82 L ATOM 73 OG SER 10 144.392 58.53850.289 1.00 81.38 L ATOM 74 C SER 10 142.161 59.254 51.308 1.00 31.04 LATOM 75 O SER 10 141.964 60.379 50.839 1.00 35.88 L ATOM 76 N LEU 11142.502 59.056 52.582 1.00 27.16 L ATOM 77 CA LEU 11 142.634 60.18153.498 1.00 24.50 L ATOM 78 CB LEU 11 141.258 60.780 53.780 1.00 47.56 LATOM 79 CG LEU 11 141.120 61.488 55.134 1.00 57.17 L ATOM 80 CD1 LEU 11141.752 62.878 55.063 1.00 65.58 L ATOM 81 CD2 LEU 11 139.650 61.58355.509 1.00 61.17 L ATOM 82 C LEU 11 143.308 59.884 54.831 1.00 22.63 LATOM 83 O LEU 11 142.978 58.919 55.508 1.00 25.60 L ATOM 84 N SER 12144.233 60.759 55.209 1.00 33.11 L ATOM 85 CA SER 12 144.965 60.65356.466 1.00 35.62 L ATOM 86 CB SER 12 146.465 60.745 56.201 1.00 34.92 LATOM 87 OG SER 12 147.186 60.895 57.409 1.00 29.53 L ATOM 88 C SER 12144.534 61.798 57.392 1.00 38.38 L ATOM 89 O SER 12 144.226 62.89456.928 1.00 46.48 L ATOM 90 N ALA 13 144.519 61.546 58.696 1.00 31.39 LATOM 91 CA ALA 13 144.116 62.559 59.666 1.00 34.62 L ATOM 92 CB ALA 13142.615 62.610 59.753 1.00 28.98 L ATOM 93 C ALA 13 144.706 62.17861.012 1.00 38.12 L ATOM 94 O ALA 13 144.928 61.002 61.268 1.00 41.17 LATOM 95 N SER 14 144.946 63.156 61.878 1.00 36.11 L ATOM 96 CA SER 14145.553 62.865 63.177 1.00 42.50 L ATOM 97 CB SER 14 146.477 64.01263.593 1.00 45.35 L ATOM 98 OG SER 14 147.608 63.522 64.299 1.00 51.37 LATOM 99 C SER 14 144.618 62.553 64.343 1.00 36.84 L ATOM 100 O SER 14143.395 62.586 64.217 1.00 30.77 L ATOM 101 N LEU 15 145.246 62.24665.477 1.00 42.69 L ATOM 102 CA LEU 15 144.588 61.919 66.737 1.00 51.12L ATOM 103 CB LEU 15 145.077 62.874 67.829 1.00 35.76 L ATOM 104 CG LEU15 145.549 64.244 67.331 1.00 50.27 L ATOM 105 CD1 LEU 15 144.709 65.35367.956 1.00 45.12 L ATOM 106 CD2 LEU 15 147.023 64.425 67.671 1.00 46.79L ATOM 107 C LEU 15 143.071 61.968 66.666 1.00 63.00 L ATOM 108 O LEU 15142.440 61.062 66.125 1.00 71.25 L ATOM 109 N GLY 16 142.495 63.02967.229 1.00 61.62 L ATOM 110 CA GLY 16 141.052 63.182 67.225 1.00 58.47L ATOM 111 C GLY 16 140.588 64.176 66.177 1.00 62.05 L ATOM 112 O GLY 16139.827 65.094 66.481 1.00 59.73 L ATOM 113 N ASP 17 141.052 63.99164.942 1.00 62.73 L ATOM 114 CA ASP 17 140.690 64.863 63.828 1.00 55.42L ATOM 115 CB ASP 17 141.729 64.728 62.715 1.00 45.39 L ATOM 116 CG ASP17 142.006 66.039 62.017 1.00 56.02 L ATOM 117 OD1 ASP 17 142.467 66.99262.682 1.00 51.84 L ATOM 118 OD2 ASP 17 141.762 66.116 60.795 1.00 64.45L ATOM 119 C ASP 17 139.313 64.470 63.298 1.00 54.85 L ATOM 120 O ASP 17139.066 63.296 63.027 1.00 69.38 L ATOM 121 N THR 18 138.425 65.44863.150 1.00 39.55 L ATOM 122 CA THR 18 137.073 65.189 62.661 1.00 35.18L ATOM 123 CB THR 18 136.089 66.238 63.211 1.00 39.72 L ATOM 124 OG1 THR18 136.155 66.250 64.644 1.00 36.21 L ATOM 125 CG2 THR 18 134.668 65.91862.774 1.00 28.85 L ATOM 126 C THR 18 136.967 65.172 61.132 1.00 39.85 LATOM 127 O THR 18 136.590 66.167 60.522 1.00 51.97 L ATOM 128 N ILE 19137.289 64.035 60.521 1.00 38.56 L ATOM 129 CA ILE 19 137.233 63.88159.065 1.00 40.49 L ATOM 130 CB ILE 19 137.917 62.579 58.621 1.00 43.22L ATOM 131 CG2 ILE 19 139.334 62.545 59.144 1.00 53.54 L ATOM 132 CG1ILE 19 137.148 61.373 59.161 1.00 31.99 L ATOM 133 CD1 ILE 19 136.02060.889 58.260 1.00 27.22 L ATOM 134 C ILE 19 135.811 63.857 58.519 1.0041.63 L ATOM 135 O ILE 19 134.850 63.825 59.284 1.00 48.28 L ATOM 136 NTHR 20 135.690 63.839 57.191 1.00 41.74 L ATOM 137 CA THR 20 134.38863.814 56.517 1.00 46.28 L ATOM 138 CB THR 20 133.761 65.246 56.473 1.0055.85 L ATOM 139 OG1 THR 20 132.666 65.316 57.396 1.00 68.15 L ATOM 140CG2 THR 20 133.260 65.592 55.073 1.00 57.90 L ATOM 141 C THR 20 134.51663.260 55.090 1.00 34.62 L ATOM 142 O THR 20 135.433 63.620 54.351 1.0035.05 L ATOM 143 N ILE 21 133.592 62.385 54.706 1.00 27.66 L ATOM 144 CAILE 21 133.613 61.793 53.371 1.00 40.68 L ATOM 145 CB ILE 21 133.64660.260 53.466 1.00 45.17 L ATOM 146 CG2 ILE 21 133.946 59.641 52.1101.00 58.28 L ATOM 147 CG1 ILE 21 134.757 59.849 54.432 1.00 37.17 L ATOM148 CD1 ILE 21 134.556 58.489 55.038 1.00 30.25 L ATOM 149 C ILE 21132.412 62.266 52.552 1.00 41.14 L ATOM 150 O ILE 21 131.706 63.17352.978 1.00 52.31 L ATOM 151 N THR 22 132.176 61.669 51.386 1.00 37.16 LATOM 152 CA THR 22 131.069 62.096 50.533 1.00 30.97 L ATOM 153 CB THR 22131.426 63.392 49.791 1.00 33.17 L ATOM 154 OG1 THR 22 131.754 64.41750.736 1.00 20.87 L ATOM 155 CG2 THR 22 130.270 63.832 48.913 1.00 26.45L ATOM 156 C THR 22 130.703 61.078 49.462 1.00 45.82 L ATOM 157 O THR 22131.563 60.656 48.694 1.00 54.24 L ATOM 158 N CYS 23 129.430 60.70549.381 1.00 50.07 L ATOM 159 CA CYS 23 129.004 59.746 48.363 1.00 63.67L ATOM 160 C CYS 23 127.790 60.230 47.570 1.00 68.93 L ATOM 161 O CYS 23126.651 60.017 47.983 1.00 85.32 L ATOM 162 CB CYS 23 128.682 58.38448.998 1.00 53.28 L ATOM 163 SG CYS 23 127.931 57.193 47.829 1.00 60.78L ATOM 164 N HIS 24 128.035 60.874 46.432 1.00 62.30 L ATOM 165 CA HIS24 126.958 61.376 45.581 1.00 55.79 L ATOM 166 CB HIS 24 127.438 62.58544.766 1.00 66.11 L ATOM 167 CG HIS 24 127.210 63.903 45.443 1.00 94.47L ATOM 168 CD2 HIS 24 127.360 64.280 46.736 1.00 100.00 L ATOM 169 ND1HIS 24 126.760 65.020 44.771 1.00 99.99 L ATOM 170 CE1 HIS 24 126.64766.029 45.618 1.00 96.64 L ATOM 171 NE2 HIS 24 127.005 65.605 46.8181.00 100.00 L ATOM 172 C HIS 24 126.456 60.282 44.636 1.00 53.07 L ATOM173 O HIS 24 127.232 59.442 44.174 1.00 50.30 L ATOM 174 N ALA 25125.154 60.295 44.359 1.00 48.84 L ATOM 175 CA ALA 25 124.546 59.30943.470 1.00 62.65 L ATOM 176 CB ALA 25 123.334 58.678 44.149 1.00 50.68L ATOM 177 C ALA 25 124.135 59.923 42.124 1.00 71.88 L ATOM 178 O ALA 25123.769 61.098 42.053 1.00 69.35 L ATOM 179 N SER 26 124.196 59.12041.061 1.00 75.26 L ATOM 180 CA SER 26 123.833 59.585 39.724 1.00 65.26L ATOM 181 CB SER 26 123.832 58.421 38.724 1.00 51.17 L ATOM 182 OG SER26 122.779 57.511 38.981 1.00 34.32 L ATOM 183 C SER 26 122.466 60.24739.755 1.00 68.59 L ATOM 184 O SER 26 122.329 61.422 39.413 1.00 70.72 LATOM 185 N GLN 27 121.452 59.490 40.163 1.00 70.13 L ATOM 186 CA GLN 27120.103 60.030 40.253 1.00 61.69 L ATOM 187 CB GLN 27 119.171 59.31239.273 1.00 49.30 L ATOM 188 CG GLN 27 119.385 57.827 39.181 1.00 40.46L ATOM 189 CD GLN 27 118.295 57.064 39.899 1.00 47.76 L ATOM 190 OE1 GLN27 117.623 57.601 40.785 1.00 42.99 L ATOM 191 NE2 GLN 27 118.110 55.80639.523 1.00 37.30 L ATOM 192 C GLN 27 119.568 59.937 41.690 1.00 59.24 LATOM 193 O GLN 27 120.323 59.658 42.623 1.00 58.99 L ATOM 194 N ASN 28118.268 60.160 41.859 1.00 54.50 L ATOM 195 CA ASN 28 117.628 60.16243.176 1.00 48.17 L ATOM 196 CB ASN 28 116.286 60.871 43.058 1.00 28.87L ATOM 197 CG ASN 28 115.671 61.159 44.397 1.00 25.62 L ATOM 198 OD1 ASN28 116.346 61.607 45.321 1.00 43.90 L ATOM 199 ND2 ASN 28 114.383 60.89444.518 1.00 47.96 L ATOM 200 C ASN 28 117.422 58.833 43.917 1.00 53.06 LATOM 201 O ASN 28 116.727 57.939 43.431 1.00 50.76 L ATOM 202 N ILE 29118.011 58.727 45.112 1.00 49.78 L ATOM 203 CA ILE 29 117.887 57.52845.951 1.00 48.76 L ATOM 204 CB ILE 29 119.278 56.900 46.275 1.00 38.91L ATOM 205 CG2 ILE 29 119.870 56.276 45.028 1.00 38.09 L ATOM 206 CG1ILE 29 120.235 57.965 46.801 1.00 35.93 L ATOM 207 CD1 ILE 29 121.44457.381 47.498 1.00 24.13 L ATOM 208 C ILE 29 117.176 57.883 47.270 1.0049.01 L ATOM 209 O ILE 29 117.043 57.056 48.183 1.00 28.15 L ATOM 210 NTYR 30 116.720 59.127 47.358 1.00 51.14 L ATOM 211 CA TYR 30 116.01859.610 48.536 1.00 40.55 L ATOM 212 CB TYR 30 114.708 58.834 48.708 1.0051.35 L ATOM 213 CG TYR 30 113.856 58.829 47.452 1.00 50.82 L ATOM 214CD1 TYR 30 112.914 59.832 47.218 1.00 57.07 L ATOM 215 CE1 TYR 30112.164 59.859 46.044 1.00 48.93 L ATOM 216 CD2 TYR 30 114.023 57.84646.477 1.00 54.94 L ATOM 217 CE2 TYR 30 113.280 57.863 45.302 1.00 54.60L ATOM 218 CZ TYR 30 112.354 58.872 45.089 1.00 54.60 L ATOM 219 OH TYR30 111.641 58.906 43.914 1.00 46.05 L ATOM 220 C TYR 30 116.867 59.53949.800 1.00 36.49 L ATOM 221 O TYR 30 117.747 60.377 50.007 1.00 36.08 LATOM 222 N VAL 31 116.607 58.553 50.650 1.00 27.22 L ATOM 223 CA VAL 31117.363 58.421 51.889 1.00 25.88 L ATOM 224 CB VAL 31 116.518 58.77153.103 1.00 24.60 L ATOM 225 CG1 VAL 31 115.741 60.036 52.827 1.00 39.18L ATOM 226 CG2 VAL 31 115.581 57.607 53.441 1.00 11.43 L ATOM 227 C VAL31 117.815 57.001 52.068 1.00 30.74 L ATOM 228 O VAL 31 118.333 56.63753.125 1.00 32.26 L ATOM 229 N TRP 32 117.612 56.202 51.028 1.00 34.27 LATOM 230 CA TRP 32 117.978 54.796 51.064 1.00 29.66 L ATOM 231 CB TRP 32117.016 53.999 50.173 1.00 37.57 L ATOM 232 CG TRP 32 115.569 54.30650.497 1.00 45.16 L ATOM 233 CD2 TRP 32 114.858 53.921 51.677 1.00 28.80L ATOM 234 CE2 TRP 32 113.571 54.493 51.597 1.00 36.20 L ATOM 235 CE3TRP 32 115.183 53.149 52.798 1.00 25.93 L ATOM 236 CD1 TRP 32 114.70655.074 49.765 1.00 47.52 L ATOM 237 NE1 TRP 32 113.504 55.193 50.4181.00 36.87 L ATOM 238 CZ2 TRP 32 112.610 54.319 52.591 1.00 25.25 L ATOM239 CZ3 TRP 32 114.229 52.978 53.788 1.00 21.98 L ATOM 240 CH2 TRP 32112.954 53.563 53.677 1.00 2.00 L ATOM 241 C TRP 32 119.419 54.57850.645 1.00 17.38 L ATOM 242 O TRP 32 119.697 54.267 49.491 1.00 20.83 LATOM 243 N LEU 33 120.332 54.750 51.599 1.00 19.97 L ATOM 244 CA LEU 33121.763 54.570 51.357 1.00 39.54 L ATOM 245 CB LEU 33 122.352 55.79050.642 1.00 44.19 L ATOM 246 CG LEU 33 123.876 55.803 50.500 1.00 34.32L ATOM 247 CD1 LEU 33 124.252 56.190 49.078 1.00 27.47 L ATOM 248 CD2LEU 33 124.479 56.774 51.515 1.00 19.98 L ATOM 249 C LEU 33 122.48154.371 52.685 1.00 39.15 L ATOM 250 O LEU 33 122.345 55.182 53.595 1.0042.09 L ATOM 251 N ASN 34 123.262 53.303 52.785 1.00 38.96 L ATOM 252 CAASN 34 123.969 53.008 54.017 1.00 33.81 L ATOM 253 CB ASN 34 123.63551.588 54.470 1.00 29.67 L ATOM 254 CG ASN 34 122.323 51.104 53.908 1.0018.96 L ATOM 255 OD1 ASN 34 121.252 51.484 54.384 1.00 22.09 L ATOM 256ND2 ASN 34 122.396 50.269 52.883 1.00 22.68 L ATOM 257 C ASN 34 125.46553.162 53.889 1.00 27.35 L ATOM 258 O ASN 34 125.996 53.215 52.785 1.0025.60 L ATOM 259 N TRP 35 126.125 53.242 55.041 1.00 31.00 L ATOM 260 CATRP 35 127.574 53.380 55.122 1.00 37.04 L ATOM 261 CB TRP 35 127.94254.663 55.871 1.00 44.59 L ATOM 262 CG TRP 35 127.725 55.956 55.099 1.0050.73 L ATOM 263 CD2 TRP 35 128.624 56.560 54.155 1.00 39.25 L ATOM 264CE2 TRP 35 128.042 57.776 53.738 1.00 36.81 L ATOM 265 CE3 TRP 35129.868 56.194 53.621 1.00 29.95 L ATOM 266 CD1 TRP 35 126.660 56.80855.208 1.00 38.58 L ATOM 267 NE1 TRP 35 126.843 57.900 54.397 1.00 40.14L ATOM 268 CZ2 TRP 35 128.655 58.629 52.812 1.00 36.05 L ATOM 269 CZ3TRP 35 130.479 57.045 52.698 1.00 39.51 L ATOM 270 CH2 TRP 35 129.87158.247 52.307 1.00 31.94 L ATOM 271 C TRP 35 128.118 52.168 55.885 1.0032.85 L ATOM 272 O TRP 35 127.499 51.715 56.849 1.00 24.56 L ATOM 273 NTYR 36 129.271 51.654 55.451 1.00 29.03 L ATOM 274 CA TYR 36 129.89450.488 56.080 1.00 24.01 L ATOM 275 CB TYR 36 129.760 49.256 55.178 1.0024.57 L ATOM 276 CG TYR 36 128.341 48.844 54.845 1.00 36.78 L ATOM 277CD1 TYR 36 127.740 47.745 55.469 1.00 45.66 L ATOM 278 CE1 TYR 36126.439 47.350 55.138 1.00 29.26 L ATOM 279 CD2 TYR 36 127.606 49.53653.885 1.00 43.28 L ATOM 280 CE2 TYR 36 126.314 49.153 53.550 1.00 29.25L ATOM 281 CZ TYR 36 125.736 48.064 54.174 1.00 33.13 L ATOM 282 OH TYR36 124.458 47.709 53.810 1.00 29.39 L ATOM 283 C TYR 36 131.379 50.68356.401 1.00 34.79 L ATOM 284 O TYR 36 132.060 51.530 55.813 1.00 31.84 LATOM 285 N GLN 37 131.876 49.880 57.337 1.00 38.75 L ATOM 286 CA GLN 37133.278 49.923 57.731 1.00 41.58 L ATOM 287 CB GLN 37 133.406 50.34259.199 1.00 30.71 L ATOM 288 CG GLN 37 134.852 50.495 59.704 1.00 37.19L ATOM 289 CD GLN 37 134.989 50.332 61.222 1.00 25.39 L ATOM 290 OE1 GLN37 134.904 49.222 61.753 1.00 36.25 L ATOM 291 NE2 GLN 37 135.209 51.43961.918 1.00 22.58 L ATOM 292 C GLN 37 133.884 48.528 57.528 1.00 39.79 LATOM 293 O GLN 37 133.319 47.526 57.975 1.00 29.52 L ATOM 294 N GLN 38135.022 48.465 56.839 1.00 29.65 L ATOM 295 CA GLN 38 135.695 47.19556.596 1.00 31.00 L ATOM 296 CB GLN 38 135.471 46.738 55.155 1.00 37.06L ATOM 297 CG GLN 38 136.385 45.597 54.724 1.00 37.85 L ATOM 298 CD GLN38 135.973 44.992 53.401 1.00 51.91 L ATOM 299 OE1 GLN 38 135.856 45.69352.399 1.00 56.78 L ATOM 300 NE2 GLN 38 135.753 43.683 53.390 1.00 47.92L ATOM 301 C GLN 38 137.192 47.285 56.860 1.00 23.61 L ATOM 302 O GLN 38137.952 47.630 55.963 1.00 30.06 L ATOM 303 N LYS 39 137.610 46.96958.086 1.00 29.96 L ATOM 304 CA LYS 39 139.021 47.022 58.464 1.00 20.79L ATOM 305 CB LYS 39 139.204 46.606 59.925 1.00 26.60 L ATOM 306 CG LYS39 139.493 47.764 60.884 1.00 31.56 L ATOM 307 CD LYS 39 138.268 48.13561.727 1.00 24.64 L ATOM 308 CE LYS 39 138.633 48.266 63.205 1.00 28.08L ATOM 309 NZ LYS 39 137.922 47.293 64.094 1.00 32.42 L ATOM 310 C LYS39 139.831 46.103 57.566 1.00 39.66 L ATOM 311 O LYS 39 139.272 45.23856.886 1.00 49.56 L ATOM 312 N PRO 40 141.161 46.283 57.533 1.00 54.59 LATOM 313 CD PRO 40 141.957 47.289 58.266 1.00 52.22 L ATOM 314 CA PRO 40142.007 45.434 56.686 1.00 54.43 L ATOM 315 CB PRO 40 143.431 45.87457.037 1.00 40.87 L ATOM 316 CG PRO 40 143.274 47.280 57.530 1.00 36.77L ATOM 317 C PRO 40 141.792 43.935 56.895 1.00 53.33 L ATOM 318 O PRO 40141.962 43.429 58.003 1.00 48.18 L ATOM 319 N GLY 41 141.403 43.24455.825 1.00 56.47 L ATOM 320 CA GLY 41 141.178 41.807 55.889 1.00 46.36L ATOM 321 C GLY 41 140.030 41.378 56.783 1.00 47.51 L ATOM 322 O GLY 41139.890 40.195 57.103 1.00 42.82 L ATOM 323 N ASN 42 139.198 42.33657.177 1.00 39.14 L ATOM 324 CA ASN 42 138.061 42.049 58.044 1.00 33.16L ATOM 325 CB ASN 42 138.031 43.041 59.210 1.00 25.23 L ATOM 326 CG ASN42 137.400 42.453 60.451 1.00 34.65 L ATOM 327 OD1 ASN 42 136.876 41.33860.416 1.00 42.65 L ATOM 328 ND2 ASN 42 137.444 43.194 61.558 1.00 15.22L ATOM 329 C ASN 42 136.743 42.123 57.287 1.00 36.43 L ATOM 330 O ASN 42136.726 42.413 56.092 1.00 31.10 L ATOM 331 N ILE 43 135.648 41.85257.993 1.00 46.04 L ATOM 332 CA ILE 43 134.310 41.894 57.407 1.00 55.78L ATOM 333 CB ILE 43 133.340 40.879 58.089 1.00 55.57 L ATOM 334 CG2 ILE43 134.038 39.554 58.323 1.00 47.83 L ATOM 335 CG1 ILE 43 132.828 41.43959.423 1.00 61.00 L ATOM 336 CD1 ILE 43 131.656 40.662 60.017 1.00 68.36L ATOM 337 C ILE 43 133.726 43.298 57.555 1.00 63.83 L ATOM 338 O ILE 43134.037 44.010 58.513 1.00 72.13 L ATOM 339 N PRO 44 132.881 43.72056.605 1.00 56.85 L ATOM 340 CD PRO 44 132.444 42.988 55.402 1.00 59.76L ATOM 341 CA PRO 44 132.282 45.052 56.681 1.00 47.82 L ATOM 342 CB PRO44 131.893 45.349 55.242 1.00 52.95 L ATOM 343 CG PRO 44 131.590 43.99754.665 1.00 66.17 L ATOM 344 C PRO 44 131.079 45.064 57.613 1.00 41.70 LATOM 345 O PRO 44 130.160 44.258 57.460 1.00 35.22 L ATOM 346 N LYS 45131.095 45.982 58.575 1.00 33.96 L ATOM 347 CA LYS 45 130.010 46.10659.537 1.00 39.65 L ATOM 348 CB LYS 45 130.576 46.225 60.953 1.00 36.05L ATOM 349 CG LYS 45 132.096 46.222 61.012 1.00 44.96 L ATOM 350 CD LYS45 132.651 45.003 61.752 1.00 53.54 L ATOM 351 CE LYS 45 134.060 45.26962.297 1.00 58.35 L ATOM 352 NZ LYS 45 134.818 44.011 62.586 1.00 46.04L ATOM 353 C LYS 45 129.162 47.329 59.218 1.00 37.07 L ATOM 354 O LYS 45129.686 48.369 58.817 1.00 37.21 L ATOM 355 N LEU 46 127.850 47.20559.385 1.00 22.26 L ATOM 356 CA LEU 46 126.969 48.326 59.107 1.00 28.40L ATOM 357 CB LEU 46 125.505 47.904 59.239 1.00 32.99 L ATOM 358 CG LEU46 124.429 48.756 58.541 1.00 40.56 L ATOM 359 CD1 LEU 46 123.264 48.94759.508 1.00 53.21 L ATOM 360 CD2 LEU 46 124.987 50.114 58.096 1.00 41.53L ATOM 361 C LEU 46 127.268 49.459 60.079 1.00 27.28 L ATOM 362 O LEU 46127.353 49.247 61.286 1.00 40.74 L ATOM 363 N LEU 47 127.414 50.66659.548 1.00 31.54 L ATOM 364 CA LEU 47 127.719 51.837 60.362 1.00 26.31L ATOM 365 CB LEU 47 128.915 52.562 59.755 1.00 25.09 L ATOM 366 CG LEU47 130.100 52.926 60.636 1.00 16.21 L ATOM 367 CD1 LEU 47 130.727 51.67661.198 1.00 17.63 L ATOM 368 CD2 LEU 47 131.094 53.701 59.805 1.00 2.00L ATOM 369 C LEU 47 126.535 52.807 60.449 1.00 34.89 L ATOM 370 O LEU 47126.190 53.282 61.535 1.00 26.31 L ATOM 371 N ILE 48 125.934 53.10059.293 1.00 44.65 L ATOM 372 CA ILE 48 124.794 54.018 59.187 1.00 34.33L ATOM 373 CB ILE 48 125.251 55.439 58.788 1.00 24.64 L ATOM 374 CG2 ILE48 124.145 56.146 58.008 1.00 29.43 L ATOM 375 CG1 ILE 48 125.591 56.25360.033 1.00 28.98 L ATOM 376 CD1 ILE 48 126.967 56.866 59.993 1.00 25.03L ATOM 377 C ILE 48 123.775 53.576 58.133 1.00 29.31 L ATOM 378 O ILE 48124.113 53.411 56.962 1.00 40.59 L ATOM 379 N TYR 49 122.527 53.40558.547 1.00 15.91 L ATOM 380 CA TYR 49 121.485 53.018 57.613 1.00 21.27L ATOM 381 CB TYR 49 120.762 51.748 58.087 1.00 36.34 L ATOM 382 CG TYR49 119.779 51.925 59.231 1.00 35.16 L ATOM 383 CD1 TYR 49 118.408 52.06658.986 1.00 25.01 L ATOM 384 CE1 TYR 49 117.498 52.216 60.028 1.00 28.72L ATOM 385 CD2 TYR 49 120.214 51.934 60.560 1.00 28.85 L ATOM 386 CE2TYR 49 119.308 52.084 61.614 1.00 30.37 L ATOM 387 CZ TYR 49 117.95352.228 61.340 1.00 42.99 L ATOM 388 OH TYR 49 117.048 52.410 62.370 1.0055.09 L ATOM 389 C TYR 49 120.510 54.172 57.486 1.00 24.72 L ATOM 390 OTYR 49 120.451 55.037 58.356 1.00 26.69 L ATOM 391 N LYS 50 119.76254.189 56.392 1.00 25.34 L ATOM 392 CA LYS 50 118.786 55.241 56.132 1.0019.61 L ATOM 393 CB LYS 50 117.611 55.134 57.108 1.00 42.59 L ATOM 394CG LYS 50 116.426 54.345 56.566 1.00 36.91 L ATOM 395 CD LYS 50 115.12455.118 56.723 1.00 40.28 L ATOM 396 CE LYS 50 114.289 54.568 57.865 1.0033.03 L ATOM 397 NZ LYS 50 112.829 54.799 57.659 1.00 38.05 L ATOM 398 CLYS 50 119.368 56.645 56.192 1.00 22.01 L ATOM 399 O LYS 50 118.69757.575 56.623 1.00 30.12 L ATOM 400 N ALA 51 120.622 56.790 55.779 1.0032.92 L ATOM 401 CA ALA 51 121.275 58.094 55.749 1.00 27.76 L ATOM 402CB ALA 51 120.346 59.117 55.100 1.00 14.79 L ATOM 403 C ALA 51 121.78758.647 57.073 1.00 25.18 L ATOM 404 O ALA 51 122.949 59.026 57.173 1.0028.58 L ATOM 405 N SER 52 120.927 58.714 58.081 1.00 22.15 L ATOM 406 CASER 52 121.347 59.270 59.362 1.00 19.39 L ATOM 407 CB SER 52 120.69060.632 59.599 1.00 25.09 L ATOM 408 OG SER 52 120.199 61.188 58.390 1.0053.81 L ATOM 409 C SER 52 121.021 58.369 60.518 1.00 20.68 L ATOM 410 OSER 52 121.232 58.729 61.674 1.00 23.62 L ATOM 411 N ASN 53 120.49757.194 60.209 1.00 25.87 L ATOM 412 CA ASN 53 120.157 56.253 61.254 1.0027.39 L ATOM 413 CB ASN 53 118.992 55.387 60.811 1.00 27.54 L ATOM 414CG ASN 53 117.668 55.895 61.323 1.00 28.84 L ATOM 415 OD1 ASN 53 116.61955.656 60.729 1.00 26.55 L ATOM 416 ND2 ASN 53 117.713 56.607 62.4501.00 36.37 L ATOM 417 C ASN 53 121.366 55.402 61.592 1.00 25.27 L ATOM418 O ASN 53 121.809 54.592 60.783 1.00 34.60 L ATOM 419 N LEU 54121.899 55.607 62.795 1.00 34.99 L ATOM 420 CA LEU 54 123.064 54.88063.294 1.00 47.12 L ATOM 421 CB LEU 54 123.649 55.607 64.510 1.00 37.02L ATOM 422 CG LEU 54 125.152 55.873 64.594 1.00 20.40 L ATOM 423 CD1 LEU54 125.508 57.117 63.830 1.00 25.30 L ATOM 424 CD2 LEU 54 125.533 56.03466.039 1.00 22.53 L ATOM 425 C LEU 54 122.660 53.468 63.705 1.00 56.63 LATOM 426 O LEU 54 121.529 53.250 64.145 1.00 68.94 L ATOM 427 N HIS 55123.578 52.513 63.573 1.00 51.79 L ATOM 428 CA HIS 55 123.267 51.14263.948 1.00 38.57 L ATOM 429 CB HIS 55 124.091 50.139 63.137 1.00 36.54L ATOM 430 CG HIS 55 123.551 48.742 63.186 1.00 27.93 L ATOM 431 CD2 HIS55 122.338 48.268 63.551 1.00 31.02 L ATOM 432 ND1 HIS 55 124.303 47.63962.841 1.00 36.74 L ATOM 433 CE1 HIS 55 123.573 46.545 62.993 1.00 43.03L ATOM 434 NE2 HIS 55 122.376 46.902 63.423 1.00 37.77 L ATOM 435 C HIS55 123.513 50.925 65.424 1.00 38.01 L ATOM 436 O HIS 55 124.296 51.63466.058 1.00 47.97 L ATOM 437 N THR 56 122.832 49.930 65.966 1.00 33.15 LATOM 438 CA THR 56 122.951 49.600 67.371 1.00 41.46 L ATOM 439 CB THR 56122.008 48.436 67.708 1.00 58.72 L ATOM 440 OG1 THR 56 121.601 47.78766.495 1.00 57.81 L ATOM 441 CG2 THR 56 120.770 48.955 68.430 1.00 63.75L ATOM 442 C THR 56 124.380 49.260 67.805 1.00 32.47 L ATOM 443 O THR 56124.940 48.236 67.415 1.00 23.36 L ATOM 444 N GLY 57 124.967 50.13668.612 1.00 28.03 L ATOM 445 CA GLY 57 126.311 49.893 69.101 1.00 36.90L ATOM 446 C GLY 57 127.388 50.703 68.403 1.00 48.73 L ATOM 447 O GLY 57128.584 50.506 68.641 1.00 45.48 L ATOM 448 N VAL 58 126.971 51.61667.537 1.00 45.32 L ATOM 449 CA VAL 58 127.926 52.439 66.819 1.00 42.70L ATOM 450 CB VAL 58 127.448 52.680 65.372 1.00 41.53 L ATOM 451 CG1 VAL58 128.383 53.648 64.654 1.00 20.54 L ATOM 452 CG2 VAL 58 127.380 51.35464.635 1.00 16.05 L ATOM 453 C VAL 58 128.129 53.768 67.546 1.00 40.96 LATOM 454 O VAL 58 127.178 54.509 67.780 1.00 35.53 L ATOM 455 N PRO 59129.378 54.059 67.951 1.00 38.91 L ATOM 456 CD PRO 59 130.553 53.18767.788 1.00 42.54 L ATOM 457 CA PRO 59 129.717 55.296 68.658 1.00 38.07L ATOM 458 CB PRO 59 131.253 55.289 68.706 1.00 34.29 L ATOM 459 CG PRO59 131.698 54.148 67.847 1.00 25.37 L ATOM 460 C PRO 59 129.174 56.54367.980 1.00 36.88 L ATOM 461 O PRO 59 129.273 56.694 66.762 1.00 38.73 LATOM 462 N SER 60 128.605 57.437 68.782 1.00 35.30 L ATOM 463 CA SER 60128.044 58.680 68.274 1.00 37.70 L ATOM 464 CB SER 60 127.466 59.51369.424 1.00 47.02 L ATOM 465 OG SER 60 128.108 59.201 70.648 1.00 44.15L ATOM 466 C SER 60 129.096 59.492 67.536 1.00 33.70 L ATOM 467 O SER 60128.760 60.405 66.785 1.00 38.05 L ATOM 468 N ARG 61 130.369 59.17567.758 1.00 31.36 L ATOM 469 CA ARG 61 131.440 59.887 67.068 1.00 40.37L ATOM 470 CB ARG 61 132.801 59.241 67.361 1.00 45.69 L ATOM 471 CG ARG61 133.316 58.277 66.278 1.00 69.44 L ATOM 472 CD ARG 61 134.854 58.26366.188 1.00 56.63 L ATOM 473 NE ARG 61 135.482 57.895 67.458 1.00 41.22L ATOM 474 CZ ARG 61 135.733 56.646 67.834 1.00 36.39 L ATOM 475 NH1 ARG61 135.411 55.637 67.041 1.00 46.50 L ATOM 476 NH2 ARG 61 136.294 56.40569.008 1.00 45.10 L ATOM 477 C ARG 61 131.137 59.822 65.573 1.00 39.44 LATOM 478 O ARG 61 131.495 60.718 64.807 1.00 31.59 L ATOM 479 N PHE 62130.468 58.744 65.176 1.00 29.20 L ATOM 480 CA PHE 62 130.083 58.53463.795 1.00 21.62 L ATOM 481 CB PHE 62 129.922 57.038 63.507 1.00 15.95L ATOM 482 CG PHE 62 131.216 56.325 63.248 1.00 28.60 L ATOM 483 CD1 PHE62 131.770 56.303 61.975 1.00 25.81 L ATOM 484 CD2 PHE 62 131.884 55.66164.277 1.00 34.71 L ATOM 485 CE1 PHE 62 132.973 55.629 61.730 1.00 25.28L ATOM 486 CE2 PHE 62 133.086 54.987 64.039 1.00 17.13 L ATOM 487 CZ PHE62 133.628 54.972 62.759 1.00 16.17 L ATOM 488 C PHE 62 128.749 59.22363.574 1.00 27.58 L ATOM 489 O PHE 62 127.812 59.041 64.347 1.00 26.02 LATOM 490 N SER 63 128.667 60.028 62.526 1.00 27.01 L ATOM 491 CA SER 63127.432 60.724 62.194 1.00 29.21 L ATOM 492 CB SER 63 127.512 62.19762.620 1.00 27.06 L ATOM 493 OG SER 63 127.604 63.059 61.501 1.00 46.67L ATOM 494 C SER 63 127.271 60.609 60.689 1.00 31.69 L ATOM 495 O SER 63128.247 60.359 59.983 1.00 35.00 L ATOM 496 N GLY 64 126.046 60.77860.203 1.00 32.44 L ATOM 497 CA GLY 64 125.796 60.677 58.775 1.00 28.03L ATOM 498 C GLY 64 124.656 61.567 58.325 1.00 36.15 L ATOM 499 O GLY 64123.678 61.753 59.050 1.00 31.20 L ATOM 500 N SER 65 124.761 62.10357.115 1.00 45.47 L ATOM 501 CA SER 65 123.723 62.993 56.612 1.00 43.02L ATOM 502 CB SER 65 124.029 64.412 57.075 1.00 39.26 L ATOM 503 OG SER65 125.378 64.487 57.510 1.00 49.79 L ATOM 504 C SER 65 123.566 62.97555.097 1.00 33.50 L ATOM 505 O SER 65 124.401 62.428 54.373 1.00 35.77 LATOM 506 N GLY 66 122.485 63.579 54.625 1.00 17.16 L ATOM 507 CA GLY 66122.258 63.641 53.196 1.00 29.10 L ATOM 508 C GLY 66 120.920 63.08152.769 1.00 35.42 L ATOM 509 O GLY 66 120.321 62.267 53.479 1.00 32.46 LATOM 510 N SER 67 120.449 63.527 51.608 1.00 24.62 L ATOM 511 CA SER 67119.184 63.062 51.061 1.00 28.74 L ATOM 512 CB SER 67 118.012 63.75751.757 1.00 46.75 L ATOM 513 OG SER 67 116.780 63.142 51.412 1.00 59.32L ATOM 514 C SER 67 119.129 63.343 49.567 1.00 32.92 L ATOM 515 O SER 67119.579 64.391 49.113 1.00 35.08 L ATOM 516 N GLY 68 118.589 62.39948.803 1.00 32.00 L ATOM 517 CA GLY 68 118.485 62.585 47.367 1.00 22.78L ATOM 518 C GLY 68 119.712 62.129 46.604 1.00 35.09 L ATOM 519 O GLY 68119.885 60.935 46.341 1.00 32.80 L ATOM 520 N THR 69 120.567 63.08646.255 1.00 36.67 L ATOM 521 CA THR 69 121.784 62.805 45.505 1.00 36.14L ATOM 522 CB THR 69 121.890 63.748 44.285 1.00 43.08 L ATOM 523 OG1 THR69 120.775 63.516 43.414 1.00 47.12 L ATOM 524 CG2 THR 69 123.175 63.50543.514 1.00 44.41 L ATOM 525 C THR 69 123.029 62.953 46.376 1.00 29.87 LATOM 526 O THR 69 123.915 62.101 46.350 1.00 38.58 L ATOM 527 N GLY 70123.082 64.022 47.160 1.00 31.63 L ATOM 528 CA GLY 70 124.234 64.25648.016 1.00 42.61 L ATOM 529 C GLY 70 124.171 63.521 49.337 1.00 36.70 LATOM 530 O GLY 70 123.119 63.471 49.965 1.00 45.53 L ATOM 531 N PHE 71125.295 62.944 49.755 1.00 38.83 L ATOM 532 CA PHE 71 125.370 62.20651.019 1.00 33.73 L ATOM 533 CB PHE 71 124.999 60.739 50.798 1.00 31.54L ATOM 534 CG PHE 71 123.602 60.540 50.263 1.00 38.58 L ATOM 535 CD1 PHE71 122.561 60.199 51.120 1.00 21.48 L ATOM 536 CD2 PHE 71 123.329 60.69748.908 1.00 26.22 L ATOM 537 CE1 PHE 71 121.278 60.021 50.641 1.00 26.37L ATOM 538 CE2 PHE 71 122.044 60.520 48.422 1.00 42.61 L ATOM 539 CZ PHE71 121.016 60.183 49.286 1.00 24.00 L ATOM 540 C PHE 71 126.774 62.29651.605 1.00 27.97 L ATOM 541 O PHE 71 127.760 62.212 50.877 1.00 31.31 LATOM 542 N THR 72 126.866 62.457 52.921 1.00 26.78 L ATOM 543 CA THR 72128.167 62.582 53.564 1.00 26.67 L ATOM 544 CB THR 72 128.520 64.06953.781 1.00 32.81 L ATOM 545 OG1 THR 72 129.493 64.187 54.826 1.00 56.77L ATOM 546 CG2 THR 72 127.288 64.856 54.164 1.00 32.03 L ATOM 547 C THR72 128.291 61.866 54.903 1.00 27.04 L ATOM 548 O THR 72 127.308 61.72255.635 1.00 23.90 L ATOM 549 N LEU 73 129.511 61.417 55.207 1.00 31.55 LATOM 550 CA LEU 73 129.816 60.722 56.459 1.00 24.92 L ATOM 551 CB LEU 73130.387 59.333 56.194 1.00 32.89 L ATOM 552 CG LEU 73 130.625 58.55157.490 1.00 34.57 L ATOM 553 CD1 LEU 73 129.287 58.045 58.021 1.00 14.98L ATOM 554 CD2 LEU 73 131.586 57.404 57.246 1.00 10.86 L ATOM 555 C LEU73 130.826 61.512 57.274 1.00 25.60 L ATOM 556 O LEU 73 131.823 62.00456.755 1.00 39.03 L ATOM 557 N THR 74 130.581 61.593 58.567 1.00 21.07 LATOM 558 CA THR 74 131.443 62.362 59.436 1.00 25.98 L ATOM 559 CB THR 74130.686 63.625 59.933 1.00 34.99 L ATOM 560 OG1 THR 74 130.181 64.35158.808 1.00 35.85 L ATOM 561 CG2 THR 74 131.588 64.524 60.747 1.00 27.11L ATOM 562 C THR 74 131.886 61.542 60.640 1.00 29.95 L ATOM 563 O THR 74131.065 60.931 61.322 1.00 36.77 L ATOM 564 N ILE 75 133.186 61.52260.896 1.00 24.95 L ATOM 565 CA ILE 75 133.706 60.813 62.052 1.00 23.49L ATOM 566 CB ILE 75 134.723 59.737 61.659 1.00 21.59 L ATOM 567 CG2 ILE75 135.597 59.401 62.847 1.00 7.06 L ATOM 568 CG1 ILE 75 133.988 58.47961.198 1.00 33.47 L ATOM 569 CD1 ILE 75 133.840 58.343 59.687 1.00 25.94L ATOM 570 C ILE 75 134.399 61.855 62.914 1.00 34.16 L ATOM 571 O ILE 75135.440 62.381 62.537 1.00 28.63 L ATOM 572 N SER 76 133.803 62.16764.059 1.00 42.00 L ATOM 573 CA SER 76 134.366 63.155 64.971 1.00 51.91L ATOM 574 CB SER 76 133.251 63.762 65.829 1.00 67.85 L ATOM 575 OG SER76 131.981 63.244 65.449 1.00 75.08 L ATOM 576 C SER 76 135.424 62.50765.865 1.00 55.79 L ATOM 577 O SER 76 135.372 61.304 66.131 1.00 54.31 LATOM 578 N SER 77 136.384 63.307 66.319 1.00 51.60 L ATOM 579 CA SER 77137.459 62.818 67.177 1.00 51.14 L ATOM 580 CB SER 77 136.993 62.78868.633 1.00 51.40 L ATOM 581 OG SER 77 136.336 63.998 68.967 1.00 52.89L ATOM 582 C SER 77 137.938 61.433 66.755 1.00 49.10 L ATOM 583 O SER 77137.515 60.422 67.311 1.00 47.48 L ATOM 584 N LEU 78 138.832 61.40665.771 1.00 46.54 L ATOM 585 CA LEU 78 139.389 60.168 65.241 1.00 32.83L ATOM 586 CB LEU 78 140.333 60.495 64.084 1.00 27.78 L ATOM 587 CG LEU78 140.423 59.481 62.950 1.00 35.44 L ATOM 588 CD1 LEU 78 139.472 59.89861.855 1.00 40.91 L ATOM 589 CD2 LEU 78 141.852 59.397 62.421 1.00 25.57L ATOM 590 C LEU 78 140.134 59.314 66.268 1.00 28.27 L ATOM 591 O LEU 78140.668 59.809 67.264 1.00 19.55 L ATOM 592 N GLN 79 140.163 58.01665.999 1.00 30.85 L ATOM 593 CA GLN 79 140.847 57.064 66.859 1.00 34.63L ATOM 594 CB GLN 79 139.855 56.338 67.763 1.00 47.40 L ATOM 595 CG GLN79 139.162 57.238 68.764 1.00 66.32 L ATOM 596 CD GLN 79 140.118 57.79369.791 1.00 51.70 L ATOM 597 OE1 GLN 79 141.184 57.229 70.022 1.00 38.18L ATOM 598 NE2 GLN 79 139.740 58.901 70.419 1.00 50.52 L ATOM 599 C GLN79 141.534 56.054 65.965 1.00 36.58 L ATOM 600 O GLN 79 141.056 55.75564.869 1.00 30.40 L ATOM 601 N PRO 80 142.663 55.504 66.430 1.00 40.78 LATOM 602 CD PRO 80 143.270 55.791 67.740 1.00 37.68 L ATOM 603 CA PRO 80143.430 54.513 65.665 1.00 41.10 L ATOM 604 CB PRO 80 144.460 53.99166.672 1.00 43.39 L ATOM 605 CG PRO 80 144.056 54.557 68.021 1.00 37.64L ATOM 606 C PRO 80 142.532 53.398 65.149 1.00 46.35 L ATOM 607 O PRO 80142.569 53.048 63.969 1.00 41.86 L ATOM 608 N GLU 81 141.721 52.85966.056 1.00 57.27 L ATOM 609 CA GLU 81 140.793 51.773 65.761 1.00 58.29L ATOM 610 CB GLU 81 139.870 51.548 66.967 1.00 54.76 L ATOM 611 CG GLU81 138.677 52.498 67.042 1.00 71.46 L ATOM 612 CD GLU 81 138.507 53.12568.419 1.00 74.52 L ATOM 613 OE1 GLU 81 137.390 53.039 68.983 1.00 60.52L ATOM 614 OE2 GLU 81 139.489 53.703 68.934 1.00 65.39 L ATOM 615 C GLU81 139.956 51.998 64.503 1.00 62.35 L ATOM 616 O GLU 81 139.618 51.05263.794 1.00 64.51 L ATOM 617 N ASP 82 139.629 53.256 64.230 1.00 63.68 LATOM 618 CA ASP 82 138.823 53.600 63.069 1.00 50.22 L ATOM 619 CB ASP 82138.299 55.024 63.212 1.00 50.14 L ATOM 620 CG ASP 82 137.729 55.28564.581 1.00 50.17 L ATOM 621 OD1 ASP 82 137.107 54.358 65.143 1.00 56.15L ATOM 622 OD2 ASP 82 137.906 56.408 65.095 1.00 62.15 L ATOM 623 C ASP82 139.583 53.462 61.761 1.00 47.40 L ATOM 624 O ASP 82 139.080 53.83760.706 1.00 44.58 L ATOM 625 N ILE 83 140.801 52.935 61.830 1.00 43.70 LATOM 626 CA ILE 83 141.586 52.738 60.621 1.00 38.44 L ATOM 627 CB ILE 83142.991 52.194 60.932 1.00 41.83 L ATOM 628 CG2 ILE 83 143.428 51.22559.846 1.00 41.86 L ATOM 629 CG1 ILE 83 143.982 53.356 61.025 1.00 52.87L ATOM 630 CD1 ILE 83 145.232 53.032 61.826 1.00 47.10 L ATOM 631 C ILE83 140.827 51.706 59.794 1.00 32.55 L ATOM 632 O ILE 83 140.588 50.59460.263 1.00 26.83 L ATOM 633 N ALA 84 140.441 52.085 58.575 1.00 26.12 LATOM 634 CA ALA 84 139.692 51.200 57.693 1.00 15.10 L ATOM 635 CB ALA 84138.453 50.702 58.406 1.00 21.07 L ATOM 636 C ALA 84 139.290 51.91356.414 1.00 12.91 L ATOM 637 O ALA 84 139.668 53.060 56.195 1.00 24.44 LATOM 638 N THR 85 138.519 51.224 55.578 1.00 19.66 L ATOM 639 CA THR 85138.025 51.774 54.320 1.00 17.95 L ATOM 640 CB THR 85 138.413 50.89853.131 1.00 21.81 L ATOM 641 OG1 THR 85 137.552 51.197 52.024 1.00 45.12L ATOM 642 CG2 THR 85 138.277 49.435 53.490 1.00 27.37 L ATOM 643 C THR85 136.505 51.809 54.410 1.00 27.74 L ATOM 644 O THR 85 135.876 50.80454.732 1.00 32.43 L ATOM 645 N TYR 86 135.914 52.960 54.116 1.00 37.25 LATOM 646 CA TYR 86 134.469 53.109 54.213 1.00 33.34 L ATOM 647 CB TYR 86134.153 54.363 55.028 1.00 25.32 L ATOM 648 CG TYR 86 134.852 54.38156.372 1.00 28.51 L ATOM 649 CD1 TYR 86 134.151 54.118 57.547 1.00 44.83L ATOM 650 CE1 TYR 86 134.801 54.071 58.789 1.00 21.36 L ATOM 651 CD2TYR 86 136.226 54.607 56.467 1.00 14.36 L ATOM 652 CE2 TYR 86 136.88454.561 57.705 1.00 22.98 L ATOM 653 CZ TYR 86 136.158 54.289 58.857 1.0022.24 L ATOM 654 OH TYR 86 136.786 54.210 60.072 1.00 25.93 L ATOM 655 CTYR 86 133.766 53.159 52.868 1.00 44.00 L ATOM 656 O TYR 86 134.16453.908 51.975 1.00 50.72 L ATOM 657 N TYR 87 132.712 52.353 52.748 1.0051.03 L ATOM 658 CA TYR 87 131.910 52.248 51.526 1.00 48.37 L ATOM 659CB TYR 87 131.795 50.780 51.108 1.00 25.20 L ATOM 660 CG TYR 87 133.10850.126 50.753 1.00 41.42 L ATOM 661 CD1 TYR 87 133.551 50.080 49.4271.00 38.02 L ATOM 662 CE1 TYR 87 134.769 49.484 49.096 1.00 42.21 L ATOM663 CD2 TYR 87 133.918 49.555 51.742 1.00 33.37 L ATOM 664 CE2 TYR 87135.137 48.957 51.418 1.00 33.81 L ATOM 665 CZ TYR 87 135.555 48.92950.096 1.00 23.95 L ATOM 666 OH TYR 87 136.771 48.380 49.779 1.00 34.33L ATOM 667 C TYR 87 130.493 52.813 51.687 1.00 52.12 L ATOM 668 O TYR 87129.948 52.838 52.786 1.00 65.19 L ATOM 669 N CYS 88 129.895 53.25050.582 1.00 47.34 L ATOM 670 CA CYS 88 128.538 53.789 50.598 1.00 32.49L ATOM 671 C CYS 88 127.715 52.920 49.661 1.00 32.31 L ATOM 672 O CYS 88128.140 52.641 48.547 1.00 39.98 L ATOM 673 CB CYS 88 128.530 55.23350.093 1.00 33.98 L ATOM 674 SG CYS 88 128.799 55.411 48.289 1.00 51.95L ATOM 675 N GLN 89 126.549 52.476 50.109 1.00 22.59 L ATOM 676 CA GLN89 125.709 51.648 49.260 1.00 21.84 L ATOM 677 CB GLN 89 125.810 50.17949.700 1.00 31.57 L ATOM 678 CG GLN 89 124.788 49.740 50.735 1.00 42.44L ATOM 679 CD GLN 89 123.613 48.982 50.132 1.00 34.76 L ATOM 680 OE1 GLN89 122.664 48.637 50.834 1.00 26.74 L ATOM 681 NE2 GLN 89 123.671 48.72348.831 1.00 46.97 L ATOM 682 C GLN 89 124.262 52.131 49.287 1.00 25.45 LATOM 683 O GLN 89 123.783 52.603 50.313 1.00 20.31 L ATOM 684 N GLN 90123.574 52.035 48.153 1.00 19.13 L ATOM 685 CA GLN 90 122.183 52.45748.096 1.00 19.46 L ATOM 686 CB GLN 90 121.882 53.162 46.769 1.00 22.24L ATOM 687 CG GLN 90 122.072 52.299 45.523 1.00 38.10 L ATOM 688 CD GLN90 120.775 51.666 45.009 1.00 30.31 L ATOM 689 OE1 GLN 90 119.701 51.85345.586 1.00 32.81 L ATOM 690 NE2 GLN 90 120.878 50.908 43.921 1.00 28.00L ATOM 691 C GLN 90 121.257 51.250 48.267 1.00 30.72 L ATOM 692 O GLN 90121.556 50.143 47.801 1.00 25.46 L ATOM 693 N GLY 91 120.137 51.47348.951 1.00 34.63 L ATOM 694 CA GLY 91 119.166 50.416 49.179 1.00 33.97L ATOM 695 C GLY 91 117.820 50.781 48.580 1.00 38.48 L ATOM 696 O GLY 91116.762 50.335 49.035 1.00 28.43 L ATOM 697 N GLN 92 117.869 51.60347.540 1.00 28.95 L ATOM 698 CA GLN 92 116.668 52.043 46.868 1.00 29.94L ATOM 699 CB GLN 92 116.841 53.489 46.418 1.00 30.37 L ATOM 700 CG GLN92 115.571 54.307 46.498 1.00 39.02 L ATOM 701 CD GLN 92 115.079 54.73645.130 1.00 41.59 L ATOM 702 OE1 GLN 92 114.039 54.274 44.663 1.00 31.97L ATOM 703 NE2 GLN 92 115.829 55.621 44.479 1.00 59.08 L ATOM 704 C GLN92 116.352 51.144 45.680 1.00 41.66 L ATOM 705 O GLN 92 115.495 50.26745.782 1.00 33.85 L ATOM 706 N THR 93 117.046 51.373 44.564 1.00 60.13 LATOM 707 CA THR 93 116.866 50.596 43.327 1.00 66.93 L ATOM 708 CB THR 93117.639 51.242 42.149 1.00 78.05 L ATOM 709 OG1 THR 93 117.362 52.64742.104 1.00 79.52 L ATOM 710 CG2 THR 93 117.233 50.610 40.829 1.00 75.70L ATOM 711 C THR 93 117.391 49.180 43.539 1.00 59.06 L ATOM 712 O THR 93118.425 49.009 44.183 1.00 58.37 L ATOM 713 N TYR 94 116.703 48.17342.993 1.00 50.92 L ATOM 714 CA TYR 94 117.127 46.791 43.209 1.00 52.81L ATOM 715 CB TYR 94 116.119 45.793 42.646 1.00 55.69 L ATOM 716 CG TYR94 115.809 44.706 43.658 1.00 64.81 L ATOM 717 CD1 TYR 94 116.742 44.36144.643 1.00 66.44 L ATOM 718 CE1 TYR 94 116.451 43.413 45.622 1.00 61.74L ATOM 719 CD2 TYR 94 114.573 44.066 43.676 1.00 65.08 L ATOM 720 CE2TYR 94 114.269 43.112 44.654 1.00 75.90 L ATOM 721 CZ TYR 94 115.21442.796 45.623 1.00 73.24 L ATOM 722 OH TYR 94 114.918 41.877 46.603 1.0071.00 L ATOM 723 C TYR 94 118.530 46.372 42.797 1.00 58.78 L ATOM 724 OTYR 94 119.146 45.561 43.487 1.00 72.46 L ATOM 725 N PRO 95 119.04546.851 41.650 1.00 50.22 L ATOM 726 CD PRO 95 118.550 47.719 40.573 1.0029.17 L ATOM 727 CA PRO 95 120.412 46.375 41.404 1.00 45.68 L ATOM 728CB PRO 95 120.673 46.762 39.946 1.00 19.42 L ATOM 729 CG PRO 95 119.78047.938 39.712 1.00 25.27 L ATOM 730 C PRO 95 121.286 47.156 42.398 1.0043.53 L ATOM 731 O PRO 95 121.936 48.131 42.027 1.00 52.07 L ATOM 732 NTYR 96 121.253 46.734 43.667 1.00 32.53 L ATOM 733 CA TYR 96 122.00047.378 44.748 1.00 29.95 L ATOM 734 CB TYR 96 122.099 46.463 45.971 1.0035.33 L ATOM 735 CG TYR 96 120.833 46.317 46.792 1.00 28.60 L ATOM 736CD1 TYR 96 120.678 45.244 47.663 1.00 33.58 L ATOM 737 CE1 TYR 96119.523 45.087 48.430 1.00 23.02 L ATOM 738 CD2 TYR 96 119.792 47.24446.701 1.00 29.21 L ATOM 739 CE2 TYR 96 118.625 47.096 47.466 1.00 29.26L ATOM 740 CZ TYR 96 118.506 46.011 48.329 1.00 29.92 L ATOM 741 OH TYR96 117.383 45.853 49.102 1.00 25.77 L ATOM 742 C TYR 96 123.392 47.71244.281 1.00 29.68 L ATOM 743 O TYR 96 123.980 46.954 43.511 1.00 37.11 LATOM 744 N THR 97 123.926 48.830 44.770 1.00 36.89 L ATOM 745 CA THR 97125.259 49.280 44.372 1.00 40.63 L ATOM 746 CB THR 97 125.153 50.18143.141 1.00 46.47 L ATOM 747 OG1 THR 97 123.945 50.951 43.228 1.00 49.45L ATOM 748 CG2 THR 97 125.123 49.342 41.874 1.00 48.22 L ATOM 749 C THR97 126.017 50.049 45.458 1.00 34.47 L ATOM 750 O THR 97 125.473 50.97546.062 1.00 27.32 L ATOM 751 N PHE 98 127.268 49.662 45.700 1.00 24.58 LATOM 752 CA PHE 98 128.097 50.338 46.689 1.00 19.63 L ATOM 753 CB PHE 98129.009 49.369 47.458 1.00 21.69 L ATOM 754 CG PHE 98 128.386 48.05147.780 1.00 22.09 L ATOM 755 CD1 PHE 98 128.078 47.719 49.098 1.00 28.14L ATOM 756 CD2 PHE 98 128.145 47.117 46.780 1.00 29.87 L ATOM 757 CE1PHE 98 127.543 46.481 49.409 1.00 20.31 L ATOM 758 CE2 PHE 98 127.61045.877 47.080 1.00 20.97 L ATOM 759 CZ PHE 98 127.308 45.556 48.397 1.0035.93 L ATOM 760 C PHE 98 128.994 51.317 45.947 1.00 27.49 L ATOM 761 OPHE 98 128.868 51.493 44.738 1.00 15.09 L ATOM 762 N GLY 99 129.91251.931 46.689 1.00 32.03 L ATOM 763 CA GLY 99 130.839 52.878 46.106 1.0037.27 L ATOM 764 C GLY 99 132.207 52.250 45.927 1.00 44.52 L ATOM 765 OGLY 99 132.319 51.038 45.717 1.00 42.71 L ATOM 766 N GLY 100 133.24653.077 46.008 1.00 42.44 L ATOM 767 CA GLY 100 134.600 52.584 45.8481.00 51.35 L ATOM 768 C GLY 100 135.344 52.448 47.164 1.00 53.12 L ATOM769 O GLY 100 136.390 51.791 47.241 1.00 49.35 L ATOM 770 N GLY 101134.799 53.067 48.204 1.00 43.66 L ATOM 771 CA GLY 101 135.430 53.01349.504 1.00 26.42 L ATOM 772 C GLY 101 136.368 54.181 49.696 1.00 21.78L ATOM 773 O GLY 101 136.710 54.886 48.747 1.00 23.28 L ATOM 774 N THR102 136.787 54.392 50.933 1.00 17.65 L ATOM 775 CA THR 102 137.68255.489 51.221 1.00 39.10 L ATOM 776 CB THR 102 136.869 56.745 51.6441.00 49.68 L ATOM 777 OG1 THR 102 137.738 57.731 52.224 1.00 42.53 LATOM 778 CG2 THR 102 135.780 56.357 52.630 1.00 55.18 L ATOM 779 C THR102 138.659 55.072 52.311 1.00 37.54 L ATOM 780 O THR 102 138.295 54.94853.478 1.00 44.36 L ATOM 781 N LYS 103 139.903 54.830 51.911 1.00 34.42L ATOM 782 CA LYS 103 140.943 54.427 52.850 1.00 33.42 L ATOM 783 CB LYS103 142.245 54.118 52.095 1.00 27.82 L ATOM 784 CG LYS 103 143.51854.210 52.937 1.00 32.59 L ATOM 785 CD LYS 103 144.038 55.649 53.0361.00 39.07 L ATOM 786 CE LYS 103 145.366 55.734 53.790 1.00 45.98 L ATOM787 NZ LYS 103 146.255 56.817 53.258 1.00 37.43 L ATOM 788 C LYS 103141.185 55.531 53.868 1.00 23.38 L ATOM 789 O LYS 103 140.842 56.68753.637 1.00 30.72 L ATOM 790 N LEU 104 141.770 55.171 55.002 1.00 32.38L ATOM 791 CA LEU 104 142.077 56.159 56.021 1.00 38.59 L ATOM 792 CB LEU104 140.904 56.368 56.966 1.00 25.66 L ATOM 793 CG LEU 104 141.37857.265 58.113 1.00 32.02 L ATOM 794 CD1 LEU 104 140.804 58.658 57.9441.00 23.79 L ATOM 795 CD2 LEU 104 140.996 56.648 59.452 1.00 29.54 LATOM 796 C LEU 104 143.300 55.784 56.841 1.00 45.89 L ATOM 797 O LEU 104143.525 54.612 57.154 1.00 45.41 L ATOM 798 N GLU 105 144.080 56.79957.197 1.00 40.34 L ATOM 799 CA GLU 105 145.280 56.597 57.979 1.00 35.75L ATOM 800 CB GLU 105 146.510 56.674 57.064 1.00 52.11 L ATOM 801 CG GLU105 147.798 57.154 57.728 1.00 69.87 L ATOM 802 CD GLU 105 148.87657.513 56.712 1.00 84.87 L ATOM 803 OE1 GLU 105 150.027 57.054 56.8751.00 87.68 L ATOM 804 OE2 GLU 105 148.578 58.251 55.748 1.00 91.43 LATOM 805 C GLU 105 145.365 57.644 59.076 1.00 23.69 L ATOM 806 O GLU 105145.640 58.804 58.808 1.00 36.93 L ATOM 807 N ILE 106 145.088 57.24160.311 1.00 29.41 L ATOM 808 CA ILE 106 145.199 58.168 61.425 1.00 38.27L ATOM 809 CB ILE 106 144.723 57.513 62.769 1.00 40.94 L ATOM 810 CG2ILE 106 145.030 56.026 62.766 1.00 31.48 L ATOM 811 CG1 ILE 106 145.38758.193 63.971 1.00 37.37 L ATOM 812 CD1 ILE 106 144.571 59.336 64.5791.00 27.37 L ATOM 813 C ILE 106 146.702 58.441 61.434 1.00 40.57 L ATOM814 O ILE 106 147.497 57.506 61.438 1.00 41.72 L ATOM 815 N LYS 107147.088 59.716 61.399 1.00 48.16 L ATOM 816 CA LYS 107 148.500 60.10061.364 1.00 42.60 L ATOM 817 CB LYS 107 148.640 61.486 60.736 1.00 20.26L ATOM 818 CG LYS 107 150.043 62.069 60.819 1.00 43.13 L ATOM 819 CD LYS107 150.034 63.572 60.590 1.00 34.99 L ATOM 820 CE LYS 107 149.96763.895 59.108 1.00 38.89 L ATOM 821 NZ LYS 107 148.581 63.791 58.5881.00 13.28 L ATOM 822 C LYS 107 149.186 60.089 62.729 1.00 51.65 L ATOM823 O LYS 107 148.643 60.592 63.713 1.00 65.04 L ATOM 824 N ARG 108150.388 59.519 62.781 1.00 52.66 L ATOM 825 CA ARG 108 151.147 59.45064.028 1.00 39.90 L ATOM 826 CB ARG 108 151.199 58.007 64.553 1.00 33.40L ATOM 827 CG ARG 108 152.127 57.083 63.771 1.00 28.54 L ATOM 828 CD ARG108 152.649 55.957 64.635 1.00 25.73 L ATOM 829 NE ARG 108 153.93456.297 65.246 1.00 34.47 L ATOM 830 CZ ARG 108 154.303 55.928 66.4711.00 34.06 L ATOM 831 NH1 ARG 108 153.489 55.205 67.224 1.00 57.79 LATOM 832 NH2 ARG 108 155.484 56.280 66.947 1.00 31.28 L ATOM 833 C ARG108 152.566 59.974 63.858 1.00 41.80 L ATOM 834 O ARG 108 153.071 60.10262.733 1.00 36.52 L ATOM 835 N ALA 109 153.193 60.271 64.996 1.00 49.39L ATOM 836 CA ALA 109 154.557 60.774 65.040 1.00 56.62 L ATOM 837 CB ALA109 155.042 60.822 66.479 1.00 33.22 L ATOM 838 C ALA 109 155.479 59.89464.209 1.00 68.35 L ATOM 839 O ALA 109 155.350 58.667 64.213 1.00 79.99L ATOM 840 N ASP 110 156.403 60.524 63.492 1.00 75.21 L ATOM 841 CA ASP110 157.350 59.784 62.673 1.00 65.78 L ATOM 842 CB ASP 110 158.39560.740 62.082 1.00 68.55 L ATOM 843 CG ASP 110 157.861 61.528 60.8891.00 84.17 L ATOM 844 OD1 ASP 110 156.656 61.399 60.580 1.00 78.11 LATOM 845 OD2 ASP 110 158.641 62.277 60.256 1.00 94.16 L ATOM 846 C ASP110 158.022 58.731 63.551 1.00 60.38 L ATOM 847 O ASP 110 158.093 58.88264.776 1.00 54.97 L ATOM 848 N ALA 111 158.487 57.651 62.932 1.00 55.72L ATOM 849 CA ALA 111 159.151 56.577 63.661 1.00 59.37 L ATOM 850 CB ALA111 158.123 55.633 64.272 1.00 55.98 L ATOM 851 C ALA 111 160.074 55.81562.730 1.00 51.95 L ATOM 852 O ALA 111 159.669 55.363 61.658 1.00 59.98L ATOM 853 N ALA 112 161.328 55.685 63.141 1.00 47.00 L ATOM 854 CA ALA112 162.318 54.977 62.348 1.00 42.20 L ATOM 855 CB ALA 112 163.71255.266 62.887 1.00 43.77 L ATOM 856 C ALA 112 162.043 53.476 62.376 1.0042.23 L ATOM 857 O ALA 112 161.447 52.957 63.325 1.00 45.43 L ATOM 858 NPRO 113 162.464 52.761 61.325 1.00 51.11 L ATOM 859 CD PRO 113 163.15353.256 60.117 1.00 52.49 L ATOM 860 CA PRO 113 162.250 51.316 61.2691.00 49.74 L ATOM 861 CB PRO 113 162.267 51.018 59.776 1.00 32.76 L ATOM862 CG PRO 113 163.223 52.029 59.220 1.00 41.64 L ATOM 863 C PRO 113163.356 50.568 61.997 1.00 45.33 L ATOM 864 O PRO 113 164.511 50.98861.974 1.00 53.16 L ATOM 865 N THR 114 163.006 49.475 62.661 1.00 36.71L ATOM 866 CA THR 114 164.009 48.675 63.341 1.00 39.49 L ATOM 867 CB THR114 163.505 48.159 64.706 1.00 34.54 L ATOM 868 OG1 THR 114 162.50447.153 64.511 1.00 38.54 L ATOM 869 CG2 THR 114 162.926 49.305 65.5181.00 31.63 L ATOM 870 C THR 114 164.322 47.515 62.406 1.00 42.88 L ATOM871 O THR 114 163.527 46.585 62.247 1.00 35.53 L ATOM 872 N VAL 115165.488 47.596 61.769 1.00 36.33 L ATOM 873 CA VAL 115 165.939 46.59460.815 1.00 42.49 L ATOM 874 CB VAL 115 166.973 47.210 59.839 1.00 46.96L ATOM 875 CG1 VAL 115 167.217 46.269 58.670 1.00 26.72 L ATOM 876 CG2VAL 115 166.470 48.555 59.338 1.00 30.46 L ATOM 877 C VAL 115 166.54445.324 61.424 1.00 38.71 L ATOM 878 O VAL 115 167.064 45.327 62.541 1.0036.49 L ATOM 879 N SER 116 166.458 44.237 60.659 1.00 44.76 L ATOM 880CA SER 116 166.988 42.939 61.053 1.00 51.12 L ATOM 881 CB SER 116165.975 42.188 61.913 1.00 55.78 L ATOM 882 OG SER 116 165.653 42.93263.068 1.00 58.23 L ATOM 883 C SER 116 167.292 42.130 59.799 1.00 44.61L ATOM 884 O SER 116 166.413 41.891 58.976 1.00 53.07 L ATOM 885 N ILE117 168.547 41.726 59.641 1.00 40.79 L ATOM 886 CA ILE 117 168.93540.929 58.487 1.00 35.02 L ATOM 887 CB ILE 117 170.299 41.393 57.9021.00 20.96 L ATOM 888 CG2 ILE 117 171.426 41.040 58.848 1.00 28.48 LATOM 889 CG1 ILE 117 170.529 40.742 56.537 1.00 16.89 L ATOM 890 CD1 ILE117 171.461 41.525 55.632 1.00 18.50 L ATOM 891 C ILE 117 169.039 39.48458.952 1.00 32.15 L ATOM 892 O ILE 117 169.467 39.212 60.076 1.00 40.81L ATOM 893 N PHE 118 168.626 38.560 58.091 1.00 28.82 L ATOM 894 CA PHE118 168.671 37.145 58.423 1.00 22.76 L ATOM 895 CB PHE 118 167.26036.601 58.654 1.00 32.60 L ATOM 896 CG PHE 118 166.589 37.174 59.8581.00 32.33 L ATOM 897 CD1 PHE 118 165.845 38.339 59.761 1.00 32.14 LATOM 898 CD2 PHE 118 166.731 36.572 61.100 1.00 30.97 L ATOM 899 CE1 PHE118 165.262 38.902 60.884 1.00 43.45 L ATOM 900 CE2 PHE 118 166.14837.128 62.230 1.00 24.60 L ATOM 901 CZ PHE 118 165.411 38.294 62.1241.00 41.49 L ATOM 902 C PHE 118 169.339 36.332 57.329 1.00 26.66 L ATOM903 O PHE 118 168.928 36.370 56.170 1.00 29.78 L ATOM 904 N PRO 119170.402 35.602 57.685 1.00 34.94 L ATOM 905 CD PRO 119 170.999 35.55959.027 1.00 32.83 L ATOM 906 CA PRO 119 171.136 34.763 56.734 1.00 37.60L ATOM 907 CB PRO 119 172.400 34.369 57.497 1.00 31.28 L ATOM 908 CG PRO119 172.428 35.261 58.732 1.00 32.18 L ATOM 909 C PRO 119 170.278 33.55156.389 1.00 36.29 L ATOM 910 O PRO 119 169.297 33.266 57.079 1.00 43.06L ATOM 911 N PRO 120 170.628 32.822 55.321 1.00 33.16 L ATOM 912 CD PRO120 171.752 33.046 54.401 1.00 35.66 L ATOM 913 CA PRO 120 169.83831.645 54.944 1.00 27.25 L ATOM 914 CB PRO 120 170.543 31.110 53.6991.00 23.84 L ATOM 915 CG PRO 120 171.345 32.258 53.186 1.00 47.85 L ATOM916 C PRO 120 169.771 30.597 56.052 1.00 47.51 L ATOM 917 O PRO 120170.408 30.733 57.097 1.00 61.24 L ATOM 918 N SER 121 168.990 29.55255.812 1.00 46.49 L ATOM 919 CA SER 121 168.836 28.481 56.781 1.00 35.79L ATOM 920 CB SER 121 167.366 28.091 56.908 1.00 39.97 L ATOM 921 OG SER121 166.848 28.512 58.153 1.00 61.95 L ATOM 922 C SER 121 169.643 27.26456.366 1.00 35.14 L ATOM 923 O SER 121 169.776 26.970 55.178 1.00 54.90L ATOM 924 N SER 122 170.190 26.566 57.352 1.00 34.00 L ATOM 925 CA SER122 170.964 25.367 57.078 1.00 43.67 L ATOM 926 CB SER 122 171.31924.661 58.385 1.00 55.34 L ATOM 927 OG SER 122 170.845 25.401 59.4961.00 66.58 L ATOM 928 C SER 122 170.038 24.496 56.265 1.00 39.48 L ATOM929 O SER 122 170.345 24.107 55.139 1.00 38.43 L ATOM 930 N GLU 123168.878 24.223 56.850 1.00 39.62 L ATOM 931 CA GLU 123 167.865 23.41256.208 1.00 42.31 L ATOM 932 CB GLU 123 166.569 23.487 57.012 1.00 49.13L ATOM 933 CG GLU 123 165.364 22.895 56.294 1.00 85.24 L ATOM 934 CD GLU123 164.237 22.520 57.240 1.00 100.00 L ATOM 935 OE1 GLU 123 164.45822.525 58.473 1.00 99.99 L ATOM 936 OE2 GLU 123 163.127 22.221 56.7421.00 99.98 L ATOM 937 C GLU 123 167.627 23.885 54.774 1.00 34.58 L ATOM938 O GLU 123 167.599 23.079 53.843 1.00 33.93 L ATOM 939 N GLN 124167.467 25.193 54.588 1.00 36.30 L ATOM 940 CA GLN 124 167.228 25.71953.254 1.00 21.55 L ATOM 941 CB GLN 124 166.875 27.207 53.289 1.00 32.12L ATOM 942 CG GLN 124 167.075 27.854 51.927 1.00 35.77 L ATOM 943 CD GLN124 166.351 29.160 51.766 1.00 35.92 L ATOM 944 OE1 GLN 124 166.34929.995 52.669 1.00 37.01 L ATOM 945 NE2 GLN 124 165.739 29.355 50.6031.00 40.10 L ATOM 946 C GLN 124 168.441 25.534 52.353 1.00 29.80 L ATOM947 O GLN 124 168.308 25.269 51.159 1.00 40.34 L ATOM 948 N LEU 125169.626 25.691 52.921 1.00 29.81 L ATOM 949 CA LEU 125 170.837 25.53852.142 1.00 30.50 L ATOM 950 CB LEU 125 172.047 25.907 52.998 1.00 28.95L ATOM 951 CG LEU 125 172.296 27.418 53.004 1.00 38.05 L ATOM 952 CD1LEU 125 173.275 27.794 54.099 1.00 45.58 L ATOM 953 CD2 LEU 125 172.81427.836 51.636 1.00 18.04 L ATOM 954 C LEU 125 170.952 24.108 51.630 1.0031.63 L ATOM 955 O LEU 125 171.396 23.873 50.503 1.00 28.04 L ATOM 956 NTHR 126 170.530 23.156 52.454 1.00 34.33 L ATOM 957 CA THR 126 170.57021.741 52.094 1.00 40.34 L ATOM 958 CB THR 126 169.981 20.876 53.2261.00 47.29 L ATOM 959 OG1 THR 126 170.445 21.364 54.490 1.00 38.65 LATOM 960 CG2 THR 126 170.397 19.426 53.063 1.00 58.05 L ATOM 961 C THR126 169.806 21.444 50.796 1.00 47.30 L ATOM 962 O THR 126 170.142 20.51550.063 1.00 54.95 L ATOM 963 N SER 127 168.779 22.236 50.513 1.00 57.73L ATOM 964 CA SER 127 167.985 22.042 49.308 1.00 63.60 L ATOM 965 CB SER127 166.551 22.525 49.542 1.00 63.78 L ATOM 966 OG SER 127 166.52823.787 50.186 1.00 64.66 L ATOM 967 C SER 127 168.581 22.760 48.096 1.0067.39 L ATOM 968 O SER 127 168.031 22.693 46.994 1.00 73.65 L ATOM 969 NGLY 128 169.701 23.446 48.303 1.00 60.63 L ATOM 970 CA GLY 128 170.35024.154 47.209 1.00 50.24 L ATOM 971 C GLY 128 169.838 25.563 46.947 1.0051.09 L ATOM 972 O GLY 128 170.151 26.169 45.917 1.00 49.56 L ATOM 973 NGLY 129 169.043 26.083 47.877 1.00 48.06 L ATOM 974 CA GLY 129 168.50727.425 47.731 1.00 48.39 L ATOM 975 C GLY 129 168.926 28.261 48.919 1.0045.57 L ATOM 976 O GLY 129 169.221 27.719 49.986 1.00 58.83 L ATOM 977 NALA 130 168.964 29.577 48.746 1.00 44.51 L ATOM 978 CA ALA 130 169.36830.467 49.834 1.00 42.67 L ATOM 979 CB ALA 130 170.868 30.721 49.7661.00 41.38 L ATOM 980 C ALA 130 168.612 31.784 49.784 1.00 38.53 L ATOM981 O ALA 130 168.661 32.506 48.783 1.00 43.66 L ATOM 982 N SER 131167.923 32.103 50.872 1.00 34.81 L ATOM 983 CA SER 131 167.154 33.33450.936 1.00 28.61 L ATOM 984 CB SER 131 165.662 33.019 51.035 1.00 29.28L ATOM 985 OG SER 131 165.165 32.494 49.817 1.00 65.36 L ATOM 986 C SER131 167.568 34.177 52.118 1.00 17.56 L ATOM 987 O SER 131 167.547 33.71453.259 1.00 23.33 L ATOM 988 N VAL 132 167.958 35.413 51.842 1.00 21.04L ATOM 989 CA VAL 132 168.353 36.345 52.891 1.00 22.72 L ATOM 990 CB VAL132 169.558 37.211 52.474 1.00 24.09 L ATOM 991 CG1 VAL 132 170.32137.669 53.711 1.00 37.12 L ATOM 992 CG2 VAL 132 170.468 36.420 51.5351.00 26.09 L ATOM 993 C VAL 132 167.143 37.238 53.115 1.00 27.45 L ATOM994 O VAL 132 166.609 37.836 52.179 1.00 34.77 L ATOM 995 N VAL 133166.701 37.314 54.360 1.00 21.35 L ATOM 996 CA VAL 133 165.532 38.10154.688 1.00 15.87 L ATOM 997 CB VAL 133 164.483 37.235 55.426 1.00 18.41L ATOM 998 CG1 VAL 133 163.382 38.111 56.010 1.00 41.56 L ATOM 999 CG2VAL 133 163.900 36.211 54.466 1.00 38.08 L ATOM 1000 C VAL 133 165.87039.296 55.548 1.00 10.26 L ATOM 1001 O VAL 133 166.701 39.222 56.4541.00 24.39 L ATOM 1002 N CYS 134 165.206 40.404 55.254 1.00 24.79 L ATOM1003 CA CYS 134 165.412 41.626 55.999 1.00 33.03 L ATOM 1004 C CYS 134164.070 42.163 56.444 1.00 34.77 L ATOM 1005 O CYS 134 163.166 42.33855.631 1.00 37.02 L ATOM 1006 CB CYS 134 166.104 42.660 55.127 1.0037.32 L ATOM 1007 SG CYS 134 166.705 44.083 56.077 1.00 64.48 L ATOM1008 N PHE 135 163.946 42.420 57.737 1.00 28.41 L ATOM 1009 CA PHE 135162.710 42.949 58.296 1.00 35.98 L ATOM 1010 CB PHE 135 162.297 42.15259.536 1.00 23.45 L ATOM 1011 CG PHE 135 161.854 40.746 59.244 1.0041.99 L ATOM 1012 CD1 PHE 135 160.991 40.472 58.187 1.00 58.79 L ATOM1013 CD2 PHE 135 162.280 39.696 60.049 1.00 38.90 L ATOM 1014 CE1 PHE135 160.555 39.170 57.939 1.00 56.32 L ATOM 1015 CE2 PHE 135 161.84938.391 59.810 1.00 57.18 L ATOM 1016 CZ PHE 135 160.987 38.127 58.7531.00 46.88 L ATOM 1017 C PHE 135 162.880 44.412 58.696 1.00 37.21 L ATOM1018 O PHE 135 163.841 44.773 59.373 1.00 31.75 L ATOM 1019 N LEU 136161.951 45.253 58.264 1.00 38.27 L ATOM 1020 CA LEU 136 161.968 46.66558.622 1.00 33.10 L ATOM 1021 CB LEU 136 162.049 47.531 57.369 1.0023.62 L ATOM 1022 CG LEU 136 163.303 47.259 56.534 1.00 17.58 L ATOM1023 CD1 LEU 136 163.055 46.103 55.572 1.00 17.79 L ATOM 1024 CD2 LEU136 163.686 48.512 55.770 1.00 29.81 L ATOM 1025 C LEU 136 160.63246.839 59.319 1.00 30.65 L ATOM 1026 O LEU 136 159.600 47.002 58.6731.00 30.43 L ATOM 1027 N ASN 137 160.651 46.779 60.643 1.00 35.92 L ATOM1028 CA ASN 137 159.421 46.873 61.400 1.00 43.25 L ATOM 1029 CB ASN 137159.387 45.751 62.433 1.00 42.56 L ATOM 1030 CG ASN 137 159.308 44.38461.793 1.00 30.61 L ATOM 1031 OD1 ASN 137 159.471 43.356 62.454 1.0037.72 L ATOM 1032 ND2 ASN 137 159.057 44.363 60.490 1.00 39.03 L ATOM1033 C ASN 137 159.101 48.199 62.075 1.00 40.01 L ATOM 1034 O ASN 137159.975 49.028 62.305 1.00 39.51 L ATOM 1035 N ASN 138 157.813 48.36262.370 1.00 41.07 L ATOM 1036 CA ASN 138 157.239 49.526 63.036 1.0038.43 L ATOM 1037 CB ASN 138 157.227 49.273 64.540 1.00 34.91 L ATOM1038 CG ASN 138 156.667 47.916 64.883 1.00 33.75 L ATOM 1039 OD1 ASN 138155.592 47.806 65.459 1.00 29.26 L ATOM 1040 ND2 ASN 138 157.402 46.86464.537 1.00 33.12 L ATOM 1041 C ASN 138 157.838 50.898 62.749 1.00 31.73L ATOM 1042 O ASN 138 158.582 51.447 63.559 1.00 39.37 L ATOM 1043 N PHE139 157.492 51.458 61.599 1.00 32.60 L ATOM 1044 CA PHE 139 157.98252.770 61.227 1.00 34.36 L ATOM 1045 CB PHE 139 159.138 52.644 60.2371.00 37.95 L ATOM 1046 CG PHE 139 158.770 51.972 58.946 1.00 21.99 LATOM 1047 CD1 PHE 139 158.295 52.716 57.869 1.00 28.23 L ATOM 1048 CD2PHE 139 158.941 50.597 58.792 1.00 23.34 L ATOM 1049 CE1 PHE 139 157.99852.102 56.651 1.00 22.39 L ATOM 1050 CE2 PHE 139 158.646 49.969 57.5781.00 15.56 L ATOM 1051 CZ PHE 139 158.175 50.723 56.505 1.00 21.70 LATOM 1052 C PHE 139 156.868 53.626 60.627 1.00 42.27 L ATOM 1053 O PHE139 155.772 53.142 60.350 1.00 50.50 L ATOM 1054 N TYR 140 157.15854.906 60.444 1.00 53.69 L ATOM 1055 CA TYR 140 156.205 55.847 59.8831.00 47.40 L ATOM 1056 CB TYR 140 155.193 56.266 60.942 1.00 53.56 LATOM 1057 CG TYR 140 154.002 56.979 60.360 1.00 71.75 L ATOM 1058 CD1TYR 140 154.072 58.331 60.029 1.00 76.99 L ATOM 1059 CE1 TYR 140 152.97858.991 59.471 1.00 91.66 L ATOM 1060 CD2 TYR 140 152.809 56.301 60.1191.00 85.47 L ATOM 1061 CE2 TYR 140 151.712 56.948 59.562 1.00 96.08 LATOM 1062 CZ TYR 140 151.803 58.293 59.242 1.00 93.77 L ATOM 1063 OH TYR140 150.716 58.946 58.706 1.00 99.99 L ATOM 1064 C TYR 140 156.98157.065 59.410 1.00 31.82 L ATOM 1065 O TYR 140 157.900 57.515 60.0881.00 34.79 L ATOM 1066 N PRO 141 156.631 57.613 58.240 1.00 33.26 L ATOM1067 CD PRO 141 157.366 58.764 57.691 1.00 26.57 L ATOM 1068 CA PRO 141155.566 57.178 57.336 1.00 39.66 L ATOM 1069 CB PRO 141 155.450 58.32156.323 1.00 45.22 L ATOM 1070 CG PRO 141 156.359 59.402 56.804 1.0034.28 L ATOM 1071 C PRO 141 155.882 55.860 56.651 1.00 32.49 L ATOM 1072O PRO 141 156.913 55.244 56.913 1.00 34.82 L ATOM 1073 N LYS 142 154.98655.438 55.762 1.00 33.41 L ATOM 1074 CA LYS 142 155.157 54.191 55.0331.00 45.13 L ATOM 1075 CB LYS 142 153.862 53.816 54.304 1.00 63.72 LATOM 1076 CG LYS 142 152.851 54.942 54.200 1.00 82.02 L ATOM 1077 CD LYS142 152.005 54.806 52.943 1.00 86.08 L ATOM 1078 CE LYS 142 152.03156.087 52.114 1.00 97.81 L ATOM 1079 NZ LYS 142 153.221 56.173 51.2091.00 100.00 L ATOM 1080 C LYS 142 156.290 54.308 54.029 1.00 47.94 LATOM 1081 O LYS 142 156.926 53.320 53.679 1.00 60.89 L ATOM 1082 N ASP143 156.543 55.523 53.565 1.00 61.49 L ATOM 1083 CA ASP 143 157.60555.725 52.603 1.00 70.17 L ATOM 1084 CB ASP 143 157.730 57.203 52.2591.00 86.10 L ATOM 1085 CG ASP 143 156.674 57.656 51.286 1.00 99.99 LATOM 1086 OD1 ASP 143 155.526 57.891 51.727 1.00 98.78 L ATOM 1087 OD2ASP 143 156.992 57.769 50.080 1.00 99.96 L ATOM 1088 C ASP 143 158.92355.207 53.148 1.00 62.40 L ATOM 1089 O ASP 143 159.344 55.564 54.2511.00 47.98 L ATOM 1090 N ILE 144 159.563 54.348 52.363 1.00 59.85 L ATOM1091 CA ILE 144 160.839 53.752 52.722 1.00 51.41 L ATOM 1092 CB ILE 144160.664 52.644 53.785 1.00 41.53 L ATOM 1093 CG2 ILE 144 160.074 51.39253.155 1.00 34.89 L ATOM 1094 CG1 ILE 144 162.011 52.320 54.427 1.0024.47 L ATOM 1095 CD1 ILE 144 161.892 51.470 55.671 1.00 21.68 L ATOM1096 C ILE 144 161.441 53.160 51.460 1.00 48.41 L ATOM 1097 O ILE 144160.719 52.739 50.557 1.00 31.96 L ATOM 1098 N ASN 145 162.765 53.14251.387 1.00 56.85 L ATOM 1099 CA ASN 145 163.447 52.603 50.221 1.0062.49 L ATOM 1100 CB ASN 145 164.063 53.730 49.399 1.00 75.48 L ATOM1101 CG ASN 145 164.104 53.413 47.922 1.00 98.08 L ATOM 1102 OD1 ASN 145163.684 54.221 47.094 1.00 99.97 L ATOM 1103 ND2 ASN 145 164.612 52.23147.579 1.00 99.99 L ATOM 1104 C ASN 145 164.531 51.633 50.650 1.00 52.75L ATOM 1105 O ASN 145 165.530 52.024 51.251 1.00 46.53 L ATOM 1106 N VAL146 164.324 50.362 50.330 1.00 60.86 L ATOM 1107 CA VAL 146 165.28349.327 50.683 1.00 55.40 L ATOM 1108 CB VAL 146 164.572 48.048 51.1711.00 51.60 L ATOM 1109 CG1 VAL 146 165.593 47.066 51.728 1.00 23.45 LATOM 1110 CG2 VAL 146 163.545 48.397 52.234 1.00 57.40 L ATOM 1111 C VAL146 166.158 48.975 49.490 1.00 53.23 L ATOM 1112 O VAL 146 165.70748.977 48.341 1.00 69.79 L ATOM 1113 N LYS 147 167.421 48.679 49.7751.00 49.86 L ATOM 1114 CA LYS 147 168.378 48.312 48.746 1.00 42.42 LATOM 1115 CB LYS 147 169.281 49.499 48.413 1.00 47.62 L ATOM 1116 CG LYS147 168.754 50.388 47.302 1.00 79.82 L ATOM 1117 CD LYS 147 169.89551.010 46.512 1.00 86.84 L ATOM 1118 CE LYS 147 169.939 52.524 46.6911.00 93.63 L ATOM 1119 NZ LYS 147 170.983 52.943 47.671 1.00 75.73 LATOM 1120 C LYS 147 169.222 47.159 49.265 1.00 35.56 L ATOM 1121 O LYS147 169.545 47.103 50.451 1.00 22.60 L ATOM 1122 N TRP 148 169.56646.236 48.375 1.00 43.57 L ATOM 1123 CA TRP 148 170.386 45.095 48.7581.00 34.06 L ATOM 1124 CB TRP 148 169.782 43.796 48.214 1.00 32.94 LATOM 1125 CG TRP 148 168.782 43.185 49.150 1.00 47.82 L ATOM 1126 CD2TRP 148 169.062 42.433 50.338 1.00 46.07 L ATOM 1127 CE2 TRP 148 167.82242.096 50.923 1.00 40.42 L ATOM 1128 CE3 TRP 148 170.243 42.012 50.9671.00 64.21 L ATOM 1129 CD1 TRP 148 167.420 43.270 49.065 1.00 34.71 LATOM 1130 NE1 TRP 148 166.838 42.621 50.125 1.00 38.06 L ATOM 1131 CZ2TRP 148 167.725 41.357 52.109 1.00 15.75 L ATOM 1132 CZ3 TRP 148 170.14641.277 52.148 1.00 72.60 L ATOM 1133 CH2 TRP 148 168.894 40.958 52.7051.00 49.76 L ATOM 1134 C TRP 148 171.793 45.269 48.217 1.00 36.36 L ATOM1135 O TRP 148 171.984 45.624 47.055 1.00 34.43 L ATOM 1136 N LYS 149172.784 45.027 49.062 1.00 44.04 L ATOM 1137 CA LYS 149 174.157 45.16148.623 1.00 41.49 L ATOM 1138 CB LYS 149 174.820 46.341 49.321 1.0034.89 L ATOM 1139 CG LYS 149 175.080 47.515 48.400 1.00 38.34 L ATOM1140 CD LYS 149 174.417 48.774 48.915 1.00 36.55 L ATOM 1141 CE LYS 149175.294 49.986 48.667 1.00 43.94 L ATOM 1142 NZ LYS 149 175.270 50.93049.819 1.00 55.84 L ATOM 1143 C LYS 149 174.937 43.893 48.896 1.00 38.62L ATOM 1144 O LYS 149 175.125 43.505 50.045 1.00 42.16 L ATOM 1145 N ILE150 175.372 43.241 47.825 1.00 22.84 L ATOM 1146 CA ILE 150 176.15742.020 47.938 1.00 24.90 L ATOM 1147 CB ILE 150 175.675 40.954 46.9581.00 42.14 L ATOM 1148 CG2 ILE 150 176.371 39.642 47.255 1.00 38.41 LATOM 1149 CG1 ILE 150 174.159 40.812 47.060 1.00 42.99 L ATOM 1150 CD1ILE 150 173.598 39.635 46.309 1.00 32.26 L ATOM 1151 C ILE 150 177.60042.361 47.614 1.00 23.92 L ATOM 1152 O ILE 150 177.975 42.479 46.4431.00 24.95 L ATOM 1153 N ASP 151 178.404 42.527 48.658 1.00 48.84 L ATOM1154 CA ASP 151 179.805 42.886 48.488 1.00 63.98 L ATOM 1155 CB ASP 151180.507 41.896 47.547 1.00 74.75 L ATOM 1156 CG ASP 151 180.844 40.57448.223 1.00 76.70 L ATOM 1157 OD1 ASP 151 180.681 40.465 49.460 1.0079.84 L ATOM 1158 OD2 ASP 151 181.274 39.638 47.508 1.00 56.23 L ATOM1159 C ASP 151 179.854 44.300 47.905 1.00 70.87 L ATOM 1160 O ASP 151180.483 44.536 46.868 1.00 70.42 L ATOM 1161 N GLY 152 179.172 45.22648.577 1.00 76.86 L ATOM 1162 CA GLY 152 179.139 46.613 48.139 1.0067.78 L ATOM 1163 C GLY 152 178.418 46.840 46.824 1.00 70.46 L ATOM 1164O GLY 152 178.156 47.980 46.438 1.00 79.32 L ATOM 1165 N SER 153 178.09845.754 46.131 1.00 62.96 L ATOM 1166 CA SER 153 177.409 45.845 44.8561.00 60.58 L ATOM 1167 CB SER 153 177.840 44.693 43.947 1.00 68.60 LATOM 1168 OG SER 153 178.171 45.160 42.653 1.00 71.40 L ATOM 1169 C SER153 175.902 45.800 45.063 1.00 61.38 L ATOM 1170 O SER 153 175.40145.044 45.895 1.00 59.28 L ATOM 1171 N GLU 154 175.183 46.620 44.3041.00 59.43 L ATOM 1172 CA GLU 154 173.731 46.677 44.393 1.00 66.09 LATOM 1173 CB GLU 154 173.222 47.987 43.793 1.00 81.88 L ATOM 1174 CG GLU154 172.299 48.776 44.700 1.00 78.09 L ATOM 1175 CD GLU 154 171.85750.087 44.079 1.00 85.76 L ATOM 1176 OE1 GLU 154 170.891 50.074 43.2831.00 65.69 L ATOM 1177 OE2 GLU 154 172.476 51.131 44.385 1.00 98.75 LATOM 1178 C GLU 154 173.123 45.505 43.635 1.00 78.80 L ATOM 1179 O GLU154 173.476 45.248 42.485 1.00 87.43 L ATOM 1180 N ARG 155 172.21144.791 44.288 1.00 78.61 L ATOM 1181 CA ARG 155 171.552 43.654 43.6691.00 72.18 L ATOM 1182 CB ARG 155 171.904 42.371 44.411 1.00 57.25 LATOM 1183 CG ARG 155 172.051 41.177 43.500 1.00 53.97 L ATOM 1184 CD ARG155 170.868 40.250 43.648 1.00 47.95 L ATOM 1185 NE ARG 155 170.68039.401 42.474 1.00 59.26 L ATOM 1186 CZ ARG 155 171.533 38.459 42.0841.00 47.18 L ATOM 1187 NH1 ARG 155 172.644 38.239 42.776 1.00 58.65 LATOM 1188 NH2 ARG 155 171.272 37.731 41.007 1.00 62.18 L ATOM 1189 C ARG155 170.049 43.862 43.692 1.00 76.06 L ATOM 1190 O ARG 155 169.47044.142 44.741 1.00 79.59 L ATOM 1191 N GLN 156 169.426 43.726 42.5271.00 81.46 L ATOM 1192 CA GLN 156 167.984 43.913 42.395 1.00 75.51 LATOM 1193 CB GLN 156 167.702 45.122 41.510 1.00 76.21 L ATOM 1194 CG GLN156 168.779 46.179 41.579 1.00 86.59 L ATOM 1195 CD GLN 156 168.21647.575 41.514 1.00 82.98 L ATOM 1196 OE1 GLN 156 167.460 47.912 40.5971.00 74.92 L ATOM 1197 NE2 GLN 156 168.579 48.402 42.486 1.00 79.96 LATOM 1198 C GLN 156 167.308 42.690 41.799 1.00 75.18 L ATOM 1199 O GLN156 166.094 42.663 41.629 1.00 78.45 L ATOM 1200 N ASN 157 168.10641.681 41.485 1.00 69.92 L ATOM 1201 CA ASN 157 167.577 40.460 40.9091.00 62.62 L ATOM 1202 CB ASN 157 168.587 39.880 39.912 1.00 83.40 LATOM 1203 CG ASN 157 168.058 38.656 39.189 1.00 96.73 L ATOM 1204 OD1ASN 157 168.142 37.534 39.692 1.00 99.98 L ATOM 1205 ND2 ASN 157 167.50938.865 38.000 1.00 96.79 L ATOM 1206 C ASN 157 167.270 39.440 42.0091.00 43.07 L ATOM 1207 O ASN 157 168.169 38.978 42.711 1.00 51.60 L ATOM1208 N GLY 158 165.994 39.103 42.166 1.00 37.93 L ATOM 1209 CA GLY 158165.613 38.126 43.168 1.00 45.50 L ATOM 1210 C GLY 158 165.052 38.71944.441 1.00 46.88 L ATOM 1211 O GLY 158 164.905 38.016 45.438 1.00 46.67L ATOM 1212 N VAL 159 164.730 40.005 44.409 1.00 46.40 L ATOM 1213 CAVAL 159 164.191 40.668 45.582 1.00 48.04 L ATOM 1214 CB VAL 159 164.74642.089 45.707 1.00 46.84 L ATOM 1215 CG1 VAL 159 164.497 42.616 47.1061.00 49.41 L ATOM 1216 CG2 VAL 159 166.234 42.091 45.398 1.00 31.32 LATOM 1217 C VAL 159 162.668 40.738 45.570 1.00 50.14 L ATOM 1218 O VAL159 162.065 41.111 44.563 1.00 70.43 L ATOM 1219 N LEU 160 162.06040.371 46.699 1.00 47.07 L ATOM 1220 CA LEU 160 160.606 40.379 46.8621.00 40.66 L ATOM 1221 CB LEU 160 160.060 38.958 46.894 1.00 28.04 LATOM 1222 CG LEU 160 160.148 38.197 45.577 1.00 32.43 L ATOM 1223 CD1LEU 160 159.270 36.965 45.669 1.00 37.34 L ATOM 1224 CD2 LEU 160 159.72239.095 44.413 1.00 7.94 L ATOM 1225 C LEU 160 160.204 41.077 48.151 1.0042.42 L ATOM 1226 O LEU 160 160.474 40.583 49.250 1.00 48.94 L ATOM 1227N ASN 161 159.545 42.220 48.011 1.00 40.58 L ATOM 1228 CA ASN 161159.109 42.996 49.161 1.00 26.69 L ATOM 1229 CB ASN 161 159.377 44.47748.917 1.00 24.90 L ATOM 1230 CG ASN 161 160.804 44.746 48.500 1.0036.23 L ATOM 1231 OD1 ASN 161 161.743 44.301 49.153 1.00 55.57 L ATOM1232 ND2 ASN 161 160.975 45.475 47.407 1.00 47.35 L ATOM 1233 C ASN 161157.637 42.792 49.471 1.00 32.25 L ATOM 1234 O ASN 161 156.850 42.41548.605 1.00 34.73 L ATOM 1235 N SER 162 157.276 43.052 50.722 1.00 28.82L ATOM 1236 CA SER 162 155.908 42.907 51.179 1.00 30.10 L ATOM 1237 CBSER 162 155.655 41.470 51.623 1.00 25.07 L ATOM 1238 OG SER 162 154.36841.341 52.203 1.00 34.98 L ATOM 1239 C SER 162 155.687 43.855 52.3491.00 28.22 L ATOM 1240 O SER 162 156.542 43.949 53.231 1.00 28.01 L ATOM1241 N TRP 163 154.556 44.560 52.346 1.00 44.68 L ATOM 1242 CA TRP 163154.217 45.502 53.413 1.00 47.01 L ATOM 1243 CB TRP 163 153.977 46.91452.865 1.00 47.04 L ATOM 1244 CG TRP 163 155.114 47.542 52.139 1.0050.28 L ATOM 1245 CD2 TRP 163 155.581 47.209 50.824 1.00 47.77 L ATOM1246 CE2 TRP 163 156.655 48.079 50.529 1.00 47.30 L ATOM 1247 CE3 TRP163 155.193 46.258 49.865 1.00 36.64 L ATOM 1248 CD1 TRP 163 155.89948.573 52.578 1.00 61.33 L ATOM 1249 NE1 TRP 163 156.828 48.901 51.6151.00 51.73 L ATOM 1250 CZ2 TRP 163 157.349 48.028 49.313 1.00 41.27 LATOM 1251 CZ3 TRP 163 155.882 46.206 48.655 1.00 43.64 L ATOM 1252 CH2TRP 163 156.950 47.089 48.391 1.00 31.42 L ATOM 1253 C TRP 163 152.93745.087 54.111 1.00 47.66 L ATOM 1254 O TRP 163 152.056 44.487 53.5041.00 46.95 L ATOM 1255 N THR 164 152.831 45.420 55.389 1.00 42.09 L ATOM1256 CA THR 164 151.623 45.119 56.141 1.00 39.34 L ATOM 1257 CB THR 164151.927 44.805 57.604 1.00 40.04 L ATOM 1258 OG1 THR 164 152.625 45.91158.185 1.00 45.73 L ATOM 1259 CG2 THR 164 152.770 43.552 57.720 1.0045.43 L ATOM 1260 C THR 164 150.804 46.400 56.099 1.00 33.70 L ATOM 1261O THR 164 151.268 47.424 55.597 1.00 27.93 L ATOM 1262 N ASP 165 149.58446.349 56.620 1.00 29.63 L ATOM 1263 CA ASP 165 148.739 47.533 56.6491.00 37.94 L ATOM 1264 CB ASP 165 147.261 47.141 56.673 1.00 57.52 LATOM 1265 CG ASP 165 146.784 46.574 55.352 1.00 78.24 L ATOM 1266 OD1ASP 165 147.303 47.005 54.302 1.00 92.25 L ATOM 1267 OD2 ASP 165 145.89145.698 55.364 1.00 85.65 L ATOM 1268 C ASP 165 149.078 48.282 57.9231.00 47.66 L ATOM 1269 O ASP 165 149.760 47.746 58.792 1.00 50.50 L ATOM1270 N GLN 166 148.609 49.516 58.030 1.00 47.56 L ATOM 1271 CA GLN 166148.850 50.319 59.220 1.00 42.28 L ATOM 1272 CB GLN 166 147.982 51.57059.185 1.00 44.46 L ATOM 1273 CG GLN 166 148.213 52.541 60.320 1.0029.22 L ATOM 1274 CD GLN 166 147.967 53.965 59.885 1.00 17.51 L ATOM1275 OE1 GLN 166 146.909 54.283 59.343 1.00 43.27 L ATOM 1276 NE2 GLN166 148.949 54.830 60.103 1.00 25.31 L ATOM 1277 C GLN 166 148.46249.478 60.422 1.00 38.24 L ATOM 1278 O GLN 166 147.361 48.934 60.4651.00 28.20 L ATOM 1279 N ASP 167 149.361 49.364 61.393 1.00 47.65 L ATOM1280 CA ASP 167 149.072 48.577 62.576 1.00 59.05 L ATOM 1281 CB ASP 167150.200 48.691 63.591 1.00 40.82 L ATOM 1282 CG ASP 167 150.022 47.74264.752 1.00 39.12 L ATOM 1283 OD1 ASP 167 149.966 46.517 64.510 1.0057.63 L ATOM 1284 OD2 ASP 167 149.927 48.214 65.902 1.00 58.29 L ATOM1285 C ASP 167 147.768 49.040 63.205 1.00 68.64 L ATOM 1286 O ASP 167147.482 50.237 63.274 1.00 62.87 L ATOM 1287 N SER 168 146.978 48.07963.658 1.00 81.22 L ATOM 1288 CA SER 168 145.695 48.374 64.271 1.0077.38 L ATOM 1289 CB SER 168 144.865 47.095 64.370 1.00 77.42 L ATOM1290 OG SER 168 145.397 46.080 63.532 1.00 89.64 L ATOM 1291 C SER 168145.839 48.994 65.651 1.00 79.26 L ATOM 1292 O SER 168 144.922 49.65066.139 1.00 85.04 L ATOM 1293 N LYS 169 146.990 48.792 66.282 1.00 71.82L ATOM 1294 CA LYS 169 147.210 49.330 67.617 1.00 55.95 L ATOM 1295 CBLYS 169 147.785 48.247 68.534 1.00 74.95 L ATOM 1296 CG LYS 169 147.25946.849 68.253 1.00 84.17 L ATOM 1297 CD LYS 169 148.379 45.938 67.7621.00 83.79 L ATOM 1298 CE LYS 169 147.843 44.810 66.885 1.00 85.61 LATOM 1299 NZ LYS 169 148.678 44.588 65.665 1.00 81.34 L ATOM 1300 C LYS169 148.114 50.556 67.658 1.00 46.88 L ATOM 1301 O LYS 169 147.72051.614 68.145 1.00 59.39 L ATOM 1302 N ASP 170 149.331 50.418 67.1421.00 40.42 L ATOM 1303 CA ASP 170 150.269 51.532 67.164 1.00 32.11 LATOM 1304 CB ASP 170 151.647 51.033 67.620 1.00 37.35 L ATOM 1305 CG ASP170 152.505 50.537 66.479 1.00 58.55 L ATOM 1306 OD1 ASP 170 152.20049.471 65.909 1.00 65.81 L ATOM 1307 OD2 ASP 170 153.500 51.221 66.1621.00 76.15 L ATOM 1308 C ASP 170 150.370 52.309 65.856 1.00 34.80 L ATOM1309 O ASP 170 151.246 53.159 65.693 1.00 26.65 L ATOM 1310 N SER 171149.466 52.024 64.927 1.00 29.78 L ATOM 1311 CA SER 171 149.429 52.71963.648 1.00 32.11 L ATOM 1312 CB SER 171 148.876 54.135 63.845 1.0036.91 L ATOM 1313 OG SER 171 147.612 54.105 64.486 1.00 87.97 L ATOM1314 C SER 171 150.750 52.809 62.894 1.00 34.11 L ATOM 1315 O SER 171151.004 53.807 62.223 1.00 25.60 L ATOM 1316 N THR 172 151.591 51.78362.993 1.00 30.87 L ATOM 1317 CA THR 172 152.866 51.788 62.271 1.0019.30 L ATOM 1318 CB THR 172 154.046 51.292 63.129 1.00 41.47 L ATOM1319 OG1 THR 172 153.590 50.286 64.039 1.00 61.43 L ATOM 1320 CG2 THR172 154.686 52.449 63.876 1.00 35.62 L ATOM 1321 C THR 172 152.80750.877 61.058 1.00 25.70 L ATOM 1322 O THR 172 151.812 50.189 60.8261.00 23.98 L ATOM 1323 N TYR 173 153.892 50.871 60.295 1.00 30.31 L ATOM1324 CA TYR 173 153.985 50.042 59.109 1.00 21.84 L ATOM 1325 CB TYR 173154.167 50.911 57.863 1.00 39.03 L ATOM 1326 CG TYR 173 152.916 51.66857.487 1.00 42.99 L ATOM 1327 CD1 TYR 173 151.794 50.994 57.008 1.0041.83 L ATOM 1328 CE1 TYR 173 150.621 51.678 56.709 1.00 59.78 L ATOM1329 CD2 TYR 173 152.834 53.050 57.651 1.00 48.81 L ATOM 1330 CE2 TYR173 151.663 53.745 57.355 1.00 38.39 L ATOM 1331 CZ TYR 173 150.56053.052 56.888 1.00 60.04 L ATOM 1332 OH TYR 173 149.392 53.723 56.6071.00 65.26 L ATOM 1333 C TYR 173 155.160 49.098 59.245 1.00 30.15 L ATOM1334 O TYR 173 156.089 49.350 60.009 1.00 35.37 L ATOM 1335 N SER 174155.111 48.005 58.505 1.00 26.54 L ATOM 1336 CA SER 174 156.184 47.03558.539 1.00 25.11 L ATOM 1337 CB SER 174 155.832 45.874 59.467 1.0032.70 L ATOM 1338 OG SER 174 156.004 46.246 60.824 1.00 23.41 L ATOM1339 C SER 174 156.394 46.518 57.136 1.00 28.29 L ATOM 1340 O SER 174155.475 46.515 56.314 1.00 22.73 L ATOM 1341 N MET 175 157.614 46.09356.857 1.00 29.61 L ATOM 1342 CA MET 175 157.925 45.571 55.552 1.0024.86 L ATOM 1343 CB MET 175 158.423 46.693 54.646 1.00 16.99 L ATOM1344 CG MET 175 159.379 46.232 53.575 1.00 9.33 L ATOM 1345 SD MET 175159.949 47.595 52.577 1.00 36.70 L ATOM 1346 CE MET 175 160.270 46.76151.036 1.00 45.25 L ATOM 1347 C MET 175 158.986 44.498 55.686 1.00 31.31L ATOM 1348 O MET 175 159.800 44.522 56.615 1.00 32.20 L ATOM 1349 N SER176 158.947 43.544 54.765 1.00 27.89 L ATOM 1350 CA SER 176 159.91642.462 54.737 1.00 33.91 L ATOM 1351 CB SER 176 159.263 41.137 55.1351.00 32.59 L ATOM 1352 OG SER 176 159.019 40.331 53.997 1.00 35.30 LATOM 1353 C SER 176 160.435 42.382 53.312 1.00 22.54 L ATOM 1354 O SER176 159.669 42.497 52.356 1.00 28.72 L ATOM 1355 N SER 177 161.74342.210 53.169 1.00 21.03 L ATOM 1356 CA SER 177 162.355 42.107 51.8561.00 27.57 L ATOM 1357 CB SER 177 163.231 43.320 51.574 1.00 32.40 LATOM 1358 OG SER 177 163.713 43.272 50.245 1.00 27.68 L ATOM 1359 C SER177 163.196 40.847 51.802 1.00 22.76 L ATOM 1360 O SER 177 164.04740.621 52.661 1.00 33.72 L ATOM 1361 N THR 178 162.956 40.026 50.7881.00 18.89 L ATOM 1362 CA THR 178 163.687 38.780 50.656 1.00 16.96 LATOM 1363 CB THR 178 162.740 37.580 50.740 1.00 21.10 L ATOM 1364 OG1THR 178 161.938 37.681 51.922 1.00 36.63 L ATOM 1365 CG2 THR 178 163.53336.285 50.782 1.00 22.64 L ATOM 1366 C THR 178 164.481 38.675 49.3621.00 21.04 L ATOM 1367 O THR 178 163.949 38.878 48.266 1.00 32.90 L ATOM1368 N LEU 179 165.764 38.358 49.515 1.00 23.97 L ATOM 1369 CA LEU 179166.679 38.183 48.395 1.00 27.62 L ATOM 1370 CB LEU 179 168.000 38.89948.669 1.00 25.50 L ATOM 1371 CG LEU 179 169.043 38.856 47.557 1.0025.95 L ATOM 1372 CD1 LEU 179 168.487 39.491 46.291 1.00 44.04 L ATOM1373 CD2 LEU 179 170.289 39.591 48.025 1.00 24.41 L ATOM 1374 C LEU 179166.920 36.687 48.293 1.00 22.54 L ATOM 1375 O LEU 179 167.417 36.07049.236 1.00 16.26 L ATOM 1376 N THR 180 166.558 36.110 47.153 1.00 27.81L ATOM 1377 CA THR 180 166.699 34.675 46.941 1.00 38.84 L ATOM 1378 CBTHR 180 165.343 34.060 46.531 1.00 58.84 L ATOM 1379 OG1 THR 180 164.39134.253 47.585 1.00 69.01 L ATOM 1380 CG2 THR 180 165.489 32.571 46.2431.00 65.13 L ATOM 1381 C THR 180 167.744 34.304 45.893 1.00 38.32 L ATOM1382 O THR 180 167.658 34.715 44.733 1.00 23.89 L ATOM 1383 N LEU 181168.726 33.515 46.318 1.00 50.48 L ATOM 1384 CA LEU 181 169.796 33.05945.439 1.00 54.78 L ATOM 1385 CB LEU 181 171.118 33.751 45.782 1.0057.52 L ATOM 1386 CG LEU 181 171.097 35.239 46.126 1.00 52.16 L ATOM1387 CD1 LEU 181 171.777 35.466 47.468 1.00 64.66 L ATOM 1388 CD2 LEU181 171.800 36.017 45.032 1.00 59.17 L ATOM 1389 C LEU 181 169.97831.558 45.595 1.00 45.72 L ATOM 1390 O LEU 181 169.250 30.904 46.3471.00 53.82 L ATOM 1391 N THR 182 170.964 31.021 44.882 1.00 33.32 L ATOM1392 CA THR 182 171.268 29.601 44.935 1.00 32.86 L ATOM 1393 CB THR 182171.740 29.086 43.571 1.00 35.79 L ATOM 1394 OG1 THR 182 172.994 29.69443.241 1.00 51.80 L ATOM 1395 CG2 THR 182 170.726 29.434 42.497 1.0010.97 L ATOM 1396 C THR 182 172.371 29.340 45.955 1.00 31.89 L ATOM 1397O THR 182 173.084 30.256 46.366 1.00 22.49 L ATOM 1398 N LYS 183 172.49828.086 46.369 1.00 18.19 L ATOM 1399 CA LYS 183 173.521 27.703 47.3261.00 22.39 L ATOM 1400 CB LYS 183 173.539 26.186 47.492 1.00 27.09 LATOM 1401 CG LYS 183 173.959 25.715 48.868 1.00 21.06 L ATOM 1402 CD LYS183 174.795 24.443 48.785 1.00 28.23 L ATOM 1403 CE LYS 183 175.37124.075 50.148 1.00 65.45 L ATOM 1404 NZ LYS 183 176.860 24.085 50.1511.00 66.90 L ATOM 1405 C LYS 183 174.847 28.168 46.750 1.00 30.33 L ATOM1406 O LYS 183 175.732 28.640 47.462 1.00 47.79 L ATOM 1407 N ASP 184174.957 28.044 45.436 1.00 33.23 L ATOM 1408 CA ASP 184 176.162 28.42244.729 1.00 32.28 L ATOM 1409 CB ASP 184 176.089 27.931 43.290 1.0041.02 L ATOM 1410 CG ASP 184 176.041 26.424 43.199 1.00 63.85 L ATOM1411 OD1 ASP 184 176.516 25.754 44.147 1.00 80.57 L ATOM 1412 OD2 ASP184 175.530 25.907 42.185 1.00 76.42 L ATOM 1413 C ASP 184 176.44329.910 44.747 1.00 37.31 L ATOM 1414 O ASP 184 177.250 30.379 45.5471.00 58.28 L ATOM 1415 N GLU 185 175.770 30.652 43.873 1.00 33.24 L ATOM1416 CA GLU 185 175.994 32.088 43.781 1.00 36.62 L ATOM 1417 CB GLU 185175.088 32.691 42.702 1.00 37.34 L ATOM 1418 CG GLU 185 173.899 33.48443.198 1.00 71.02 L ATOM 1419 CD GLU 185 172.973 33.876 42.058 1.0078.07 L ATOM 1420 OE1 GLU 185 173.399 34.680 41.198 1.00 94.70 L ATOM1421 OE2 GLU 185 171.826 33.378 42.020 1.00 82.48 L ATOM 1422 C GLU 185175.870 32.849 45.095 1.00 40.77 L ATOM 1423 O GLU 185 176.154 34.03945.144 1.00 28.19 L ATOM 1424 N TYR 186 175.455 32.171 46.160 1.00 45.72L ATOM 1425 CA TYR 186 175.357 32.824 47.461 1.00 31.13 L ATOM 1426 CBTYR 186 174.183 32.284 48.271 1.00 29.29 L ATOM 1427 CG TYR 186 174.39432.396 49.769 1.00 30.13 L ATOM 1428 CD1 TYR 186 174.131 33.590 50.4471.00 33.39 L ATOM 1429 CE1 TYR 186 174.314 33.694 51.833 1.00 36.37 LATOM 1430 CD2 TYR 186 174.850 31.310 50.509 1.00 34.49 L ATOM 1431 CE2TYR 186 175.036 31.402 51.893 1.00 18.79 L ATOM 1432 CZ TYR 186 174.76632.594 52.549 1.00 34.18 L ATOM 1433 OH TYR 186 174.933 32.673 53.9131.00 39.71 L ATOM 1434 C TYR 186 176.641 32.530 48.224 1.00 25.69 L ATOM1435 O TYR 186 177.138 33.358 48.985 1.00 46.65 L ATOM 1436 N GLU 187177.163 31.327 48.025 1.00 33.86 L ATOM 1437 CA GLU 187 178.380 30.91648.695 1.00 30.31 L ATOM 1438 CB GLU 187 178.478 29.394 48.707 1.0051.43 L ATOM 1439 CG GLU 187 177.688 28.757 49.831 1.00 68.32 L ATOM1440 CD GLU 187 177.931 27.269 49.944 1.00 84.34 L ATOM 1441 OE1 GLU 187178.505 26.681 49.000 1.00 72.88 L ATOM 1442 OE2 GLU 187 177.540 26.69050.980 1.00 92.94 L ATOM 1443 C GLU 187 179.608 31.516 48.021 1.00 25.34L ATOM 1444 O GLU 187 180.731 31.319 48.476 1.00 33.80 L ATOM 1445 N ARG188 179.393 32.255 46.937 1.00 25.18 L ATOM 1446 CA ARG 188 180.50432.887 46.238 1.00 28.26 L ATOM 1447 CB ARG 188 180.430 32.583 44.7321.00 50.64 L ATOM 1448 CG ARG 188 179.374 33.352 43.952 1.00 42.62 LATOM 1449 CD ARG 188 179.332 32.895 42.493 1.00 43.87 L ATOM 1450 NE ARG188 179.213 31.440 42.378 1.00 93.56 L ATOM 1451 CZ ARG 188 180.10630.659 41.779 1.00 99.99 L ATOM 1452 NH1 ARG 188 181.192 31.187 41.2301.00 99.99 L ATOM 1453 NH2 ARG 188 179.918 29.345 41.733 1.00 99.98 LATOM 1454 C ARG 188 180.549 34.399 46.490 1.00 22.65 L ATOM 1455 O ARG188 181.077 35.160 45.679 1.00 30.16 L ATOM 1456 N HIS 189 180.00134.819 47.626 1.00 27.86 L ATOM 1457 CA HIS 189 179.979 36.223 48.0231.00 21.88 L ATOM 1458 CB HIS 189 178.650 36.871 47.650 1.00 40.07 LATOM 1459 CG HIS 189 178.480 37.107 46.182 1.00 47.15 L ATOM 1460 CD2HIS 189 178.796 38.171 45.405 1.00 55.79 L ATOM 1461 ND1 HIS 189 177.89136.185 45.342 1.00 63.86 L ATOM 1462 CE1 HIS 189 177.849 36.672 44.1151.00 61.64 L ATOM 1463 NE2 HIS 189 178.391 37.876 44.127 1.00 60.31 LATOM 1464 C HIS 189 180.152 36.239 49.531 1.00 29.34 L ATOM 1465 O HIS189 179.750 35.291 50.205 1.00 34.87 L ATOM 1466 N ASN 190 180.72937.309 50.067 1.00 37.34 L ATOM 1467 CA ASN 190 180.961 37.379 51.5071.00 47.66 L ATOM 1468 CB ASN 190 182.388 37.855 51.795 1.00 68.12 LATOM 1469 CG ASN 190 183.417 37.167 50.930 1.00 100.00 L ATOM 1470 OD1ASN 190 183.407 35.945 50.784 1.00 99.97 L ATOM 1471 ND2 ASN 190 184.32037.954 50.350 1.00 99.98 L ATOM 1472 C ASN 190 180.001 38.252 52.2901.00 42.97 L ATOM 1473 O ASN 190 179.383 37.797 53.259 1.00 33.80 L ATOM1474 N SER 191 179.885 39.511 51.892 1.00 39.67 L ATOM 1475 CA SER 191179.010 40.419 52.614 1.00 30.33 L ATOM 1476 CB SER 191 179.698 41.77452.801 1.00 31.09 L ATOM 1477 OG SER 191 179.887 42.056 54.177 1.0059.06 L ATOM 1478 C SER 191 177.655 40.634 51.967 1.00 34.40 L ATOM 1479O SER 191 177.550 40.836 50.753 1.00 48.04 L ATOM 1480 N TYR 192 176.62040.574 52.802 1.00 25.05 L ATOM 1481 CA TYR 192 175.246 40.785 52.3611.00 22.04 L ATOM 1482 CB TYR 192 174.420 39.517 52.582 1.00 16.56 LATOM 1483 CG TYR 192 174.807 38.444 51.598 1.00 33.82 L ATOM 1484 CD1TYR 192 174.383 38.511 50.273 1.00 39.72 L ATOM 1485 CE1 TYR 192 174.82937.592 49.330 1.00 33.77 L ATOM 1486 CD2 TYR 192 175.683 37.417 51.9611.00 36.76 L ATOM 1487 CE2 TYR 192 176.135 36.492 51.024 1.00 36.40 LATOM 1488 CZ TYR 192 175.707 36.587 49.711 1.00 39.55 L ATOM 1489 OH TYR192 176.164 35.693 48.766 1.00 31.96 L ATOM 1490 C TYR 192 174.70541.961 53.160 1.00 26.50 L ATOM 1491 O TYR 192 174.749 41.970 54.3891.00 26.85 L ATOM 1492 N THR 193 174.205 42.959 52.443 1.00 42.59 L ATOM1493 CA THR 193 173.716 44.165 53.086 1.00 49.57 L ATOM 1494 CB THR 193174.621 45.362 52.738 1.00 59.55 L ATOM 1495 OG1 THR 193 175.983 45.02553.025 1.00 51.20 L ATOM 1496 CG2 THR 193 174.219 46.592 53.536 1.0059.94 L ATOM 1497 C THR 193 172.285 44.574 52.777 1.00 46.91 L ATOM 1498O THR 193 171.848 44.573 51.621 1.00 57.88 L ATOM 1499 N CYS 194 171.57844.946 53.836 1.00 40.06 L ATOM 1500 CA CYS 194 170.208 45.409 53.7381.00 39.66 L ATOM 1501 C CYS 194 170.229 46.894 54.090 1.00 50.91 L ATOM1502 O CYS 194 170.419 47.263 55.253 1.00 50.44 L ATOM 1503 CB CYS 194169.326 44.652 54.719 1.00 56.36 L ATOM 1504 SG CYS 194 167.606 45.21954.662 1.00 57.55 L ATOM 1505 N GLU 195 170.039 47.734 53.080 1.00 62.99L ATOM 1506 CA GLU 195 170.066 49.182 53.262 1.00 56.68 L ATOM 1507 CBGLU 195 171.006 49.802 52.231 1.00 67.85 L ATOM 1508 CG GLU 195 171.61851.121 52.650 1.00 75.89 L ATOM 1509 CD GLU 195 172.492 51.711 51.5601.00 66.08 L ATOM 1510 OE1 GLU 195 171.941 52.170 50.533 1.00 83.91 LATOM 1511 OE2 GLU 195 173.731 51.710 51.731 1.00 69.63 L ATOM 1512 C GLU195 168.689 49.812 53.128 1.00 41.96 L ATOM 1513 O GLU 195 167.92649.466 52.224 1.00 46.93 L ATOM 1514 N ALA 196 168.381 50.755 54.0141.00 39.48 L ATOM 1515 CA ALA 196 167.085 51.419 53.983 1.00 55.44 LATOM 1516 CB ALA 196 166.114 50.674 54.880 1.00 62.95 L ATOM 1517 C ALA196 167.111 52.897 54.375 1.00 56.78 L ATOM 1518 O ALA 196 167.57253.254 55.460 1.00 57.46 L ATOM 1519 N THR 197 166.606 53.748 53.4841.00 59.26 L ATOM 1520 CA THR 197 166.532 55.186 53.738 1.00 71.50 LATOM 1521 CB THR 197 166.971 56.023 52.502 1.00 77.13 L ATOM 1522 OG1THR 197 166.548 55.374 51.295 1.00 83.37 L ATOM 1523 CG2 THR 197 168.48656.185 52.483 1.00 62.62 L ATOM 1524 C THR 197 165.086 55.538 54.0861.00 73.26 L ATOM 1525 O THR 197 164.155 55.182 53.362 1.00 73.27 L ATOM1526 N HIS 198 164.901 56.233 55.200 1.00 66.28 L ATOM 1527 CA HIS 198163.570 56.617 55.655 1.00 65.29 L ATOM 1528 CB HIS 198 163.203 55.77756.889 1.00 59.65 L ATOM 1529 CG HIS 198 161.802 55.976 57.386 1.0050.05 L ATOM 1530 CD2 HIS 198 161.333 56.281 58.619 1.00 10.46 L ATOM1531 ND1 HIS 198 160.691 55.811 56.586 1.00 23.72 L ATOM 1532 CE1 HIS198 159.601 56.009 57.306 1.00 8.26 L ATOM 1533 NE2 HIS 198 159.96256.295 58.545 1.00 26.12 L ATOM 1534 C HIS 198 163.611 58.101 56.0011.00 65.53 L ATOM 1535 O HIS 198 164.642 58.601 56.446 1.00 44.38 L ATOM1536 N LYS 199 162.501 58.804 55.776 1.00 69.60 L ATOM 1537 CA LYS 199162.407 60.235 56.078 1.00 62.83 L ATOM 1538 CB LYS 199 160.940 60.66556.214 1.00 59.10 L ATOM 1539 CG LYS 199 160.168 60.725 54.908 1.0066.79 L ATOM 1540 CD LYS 199 159.541 59.376 54.566 1.00 77.11 L ATOM1541 CE LYS 199 160.458 58.543 53.670 1.00 66.29 L ATOM 1542 NZ LYS 199160.372 58.922 52.226 1.00 71.28 L ATOM 1543 C LYS 199 163.100 60.45957.409 1.00 54.65 L ATOM 1544 O LYS 199 163.853 61.418 57.596 1.00 52.78L ATOM 1545 N THR 200 162.823 59.538 58.322 1.00 59.05 L ATOM 1546 CATHR 200 163.361 59.542 59.668 1.00 72.79 L ATOM 1547 CB THR 200 162.89658.262 60.404 1.00 72.74 L ATOM 1548 OG1 THR 200 162.485 58.594 61.7321.00 81.55 L ATOM 1549 CG2 THR 200 164.002 57.222 60.439 1.00 67.03 LATOM 1550 C THR 200 164.890 59.648 59.712 1.00 77.51 L ATOM 1551 O THR200 165.479 59.774 60.785 1.00 76.37 L ATOM 1552 N SER 201 165.53659.607 58.550 1.00 82.04 L ATOM 1553 CA SER 201 166.991 59.696 58.5211.00 77.00 L ATOM 1554 CB SER 201 167.599 58.411 59.082 1.00 70.20 LATOM 1555 OG SER 201 168.828 58.678 59.728 1.00 48.40 L ATOM 1556 C SER201 167.583 59.967 57.145 1.00 75.86 L ATOM 1557 O SER 201 167.12659.436 56.129 1.00 78.72 L ATOM 1558 N THR 202 168.617 60.802 57.1281.00 79.38 L ATOM 1559 CA THR 202 169.317 61.144 55.897 1.00 85.40 LATOM 1560 CB THR 202 169.864 62.586 55.942 1.00 82.98 L ATOM 1561 OG1THR 202 170.536 62.807 57.190 1.00 58.44 L ATOM 1562 CG2 THR 202 168.72563.591 55.800 1.00 81.93 L ATOM 1563 C THR 202 170.481 60.165 55.7521.00 87.12 L ATOM 1564 O THR 202 171.106 60.069 54.697 1.00 90.15 L ATOM1565 N SER 203 170.757 59.442 56.835 1.00 85.15 L ATOM 1566 CA SER 203171.826 58.452 56.866 1.00 85.74 L ATOM 1567 CB SER 203 172.589 58.53858.194 1.00 91.67 L ATOM 1568 OG SER 203 172.049 59.540 59.041 1.0086.93 L ATOM 1569 C SER 203 171.203 57.069 56.719 1.00 87.87 L ATOM 1570O SER 203 170.682 56.508 57.686 1.00 84.65 L ATOM 1571 N PRO 204 171.24456.501 55.502 1.00 89.56 L ATOM 1572 CD PRO 204 171.858 57.072 54.2921.00 93.06 L ATOM 1573 CA PRO 204 170.672 55.176 55.244 1.00 82.77 LATOM 1574 CB PRO 204 171.269 54.772 53.888 1.00 81.38 L ATOM 1575 CG PRO204 172.262 55.855 53.525 1.00 78.75 L ATOM 1576 C PRO 204 171.00254.167 56.335 1.00 76.12 L ATOM 1577 O PRO 204 172.169 53.960 56.6561.00 79.62 L ATOM 1578 N ILE 205 169.971 53.557 56.911 1.00 69.79 L ATOM1579 CA ILE 205 170.172 52.556 57.953 1.00 59.52 L ATOM 1580 CB ILE 205168.862 52.224 58.675 1.00 59.30 L ATOM 1581 CG2 ILE 205 169.124 51.19259.763 1.00 62.73 L ATOM 1582 CG1 ILE 205 168.264 53.503 59.263 1.0051.85 L ATOM 1583 CD1 ILE 205 166.912 53.310 59.921 1.00 48.97 L ATOM1584 C ILE 205 170.711 51.294 57.294 1.00 58.53 L ATOM 1585 O ILE 205170.225 50.873 56.241 1.00 60.90 L ATOM 1586 N VAL 206 171.719 50.69157.918 1.00 53.53 L ATOM 1587 CA VAL 206 172.332 49.498 57.356 1.0041.22 L ATOM 1588 CB VAL 206 173.739 49.825 56.810 1.00 18.34 L ATOM1589 CG1 VAL 206 174.486 48.548 56.467 1.00 13.15 L ATOM 1590 CG2 VAL206 173.618 50.706 55.580 1.00 13.65 L ATOM 1591 C VAL 206 172.44248.336 58.331 1.00 44.96 L ATOM 1592 O VAL 206 172.747 48.517 59.5091.00 44.72 L ATOM 1593 N LYS 207 172.180 47.137 57.818 1.00 51.44 L ATOM1594 CA LYS 207 172.269 45.915 58.598 1.00 52.57 L ATOM 1595 CB LYS 207170.882 45.438 59.020 1.00 54.70 L ATOM 1596 CG LYS 207 170.800 45.03860.479 1.00 47.68 L ATOM 1597 CD LYS 207 171.141 46.206 61.394 1.0042.68 L ATOM 1598 CE LYS 207 170.477 46.060 62.758 1.00 53.93 L ATOM1599 NZ LYS 207 170.763 44.740 63.395 1.00 56.72 L ATOM 1600 C LYS 207172.906 44.900 57.671 1.00 51.25 L ATOM 1601 O LYS 207 172.357 44.59256.610 1.00 57.87 L ATOM 1602 N SER 208 174.072 44.396 58.059 1.00 48.56L ATOM 1603 CA SER 208 174.780 43.429 57.230 1.00 44.28 L ATOM 1604 CBSER 208 175.956 44.095 56.525 1.00 43.06 L ATOM 1605 OG SER 208 175.51145.119 55.659 1.00 64.64 L ATOM 1606 C SER 208 175.301 42.236 58.0031.00 46.92 L ATOM 1607 O SER 208 175.121 42.129 59.221 1.00 49.18 L ATOM1608 N PHE 209 175.950 41.342 57.269 1.00 47.47 L ATOM 1609 CA PHE 209176.539 40.152 57.850 1.00 48.36 L ATOM 1610 CB PHE 209 175.443 39.17058.296 1.00 58.47 L ATOM 1611 CG PHE 209 174.804 38.414 57.168 1.0068.93 L ATOM 1612 CD1 PHE 209 173.734 38.960 56.471 1.00 77.87 L ATOM1613 CD2 PHE 209 175.280 37.161 56.793 1.00 73.00 L ATOM 1614 CE1 PHE209 173.148 38.270 55.411 1.00 71.56 L ATOM 1615 CE2 PHE 209 174.70336.461 55.736 1.00 64.61 L ATOM 1616 CZ PHE 209 173.633 37.017 55.0431.00 71.74 L ATOM 1617 C PHE 209 177.481 39.498 56.851 1.00 44.99 L ATOM1618 O PHE 209 177.336 39.658 55.637 1.00 35.92 L ATOM 1619 N ASN 210178.463 38.782 57.384 1.00 56.65 L ATOM 1620 CA ASN 210 179.444 38.07956.572 1.00 60.29 L ATOM 1621 CB ASN 210 180.853 38.402 57.064 1.0070.34 L ATOM 1622 CG ASN 210 181.925 37.920 56.112 1.00 100.00 L ATOM1623 OD1 ASN 210 182.386 38.670 55.250 1.00 99.98 L ATOM 1624 ND2 ASN210 182.328 36.658 56.258 1.00 100.00 L ATOM 1625 C ASN 210 179.16536.592 56.741 1.00 63.50 L ATOM 1626 O ASN 210 178.624 36.183 57.7631.00 79.33 L ATOM 1627 N ARG 211 179.524 35.780 55.756 1.00 60.96 L ATOM1628 CA ARG 211 179.284 34.350 55.872 1.00 67.53 L ATOM 1629 CB ARG 211179.672 33.644 54.576 1.00 53.17 L ATOM 1630 CG ARG 211 178.475 33.14153.792 1.00 46.30 L ATOM 1631 CD ARG 211 178.602 33.466 52.320 1.0024.02 L ATOM 1632 NE ARG 211 179.366 32.449 51.606 1.00 24.23 L ATOM1633 CZ ARG 211 180.693 32.431 51.527 1.00 46.60 L ATOM 1634 NH1 ARG 211181.411 33.377 52.118 1.00 74.04 L ATOM 1635 NH2 ARG 211 181.307 31.46650.863 1.00 58.03 L ATOM 1636 C ARG 211 180.041 33.746 57.060 1.00 78.01L ATOM 1637 O ARG 211 181.277 33.714 57.080 1.00 62.45 L ATOM 1638 N ASN212 179.264 33.284 58.046 1.00 96.28 L ATOM 1639 CA ASN 212 179.74732.661 59.288 1.00 99.99 L ATOM 1640 CB ASN 212 181.065 31.915 59.0551.00 99.98 L ATOM 1641 CG ASN 212 181.130 30.602 59.816 1.00 100.00 LATOM 1642 OD1 ASN 212 180.343 30.360 60.738 1.00 94.94 L ATOM 1643 ND2ASN 212 182.070 29.743 59.434 1.00 99.99 L ATOM 1644 C ASN 212 179.91833.640 60.455 1.00 99.98 L ATOM 1645 O ASN 212 180.791 34.533 60.3591.00 99.99 L ATOM 1646 OT ASN 212 181.064 34.157 61.055 1.00 99.99 LATOM 1647 CB VAL 2 121.621 36.267 64.620 1.00 37.38 H ATOM 1648 CG1 VAL2 120.401 36.241 65.515 1.00 27.71 H ATOM 1649 CG2 VAL 2 122.281 37.62764.680 1.00 34.18 H ATOM 1650 C VAL 2 123.855 35.223 64.193 1.00 42.92 HATOM 1651 O VAL 2 124.332 36.300 63.840 1.00 42.57 H ATOM 1652 N VAL 2122.979 35.304 66.494 1.00 47.65 H ATOM 1653 CA VAL 2 122.610 35.16065.057 1.00 44.59 H ATOM 1654 N GLN 3 124.382 34.056 63.852 1.00 45.14 HATOM 1655 CA GLN 3 125.594 33.990 63.047 1.00 47.76 H ATOM 1656 CB GLN 3126.585 33.018 63.684 1.00 47.79 H ATOM 1657 CG GLN 3 128.013 33.21663.226 1.00 70.50 H ATOM 1658 CD GLN 3 128.654 31.926 62.768 1.00 77.13H ATOM 1659 OE1 GLN 3 128.477 30.879 63.392 1.00 89.69 H ATOM 1660 NE2GLN 3 129.403 31.990 61.671 1.00 74.80 H ATOM 1661 C GLN 3 125.32433.576 61.605 1.00 43.77 H ATOM 1662 O GLN 3 124.223 33.144 61.264 1.0028.11 H ATOM 1663 N LEU 4 126.341 33.721 60.761 1.00 43.76 H ATOM 1664CA LEU 4 126.231 33.358 59.356 1.00 47.78 H ATOM 1665 CB LEU 4 126.06134.620 58.502 1.00 41.23 H ATOM 1666 CG LEU 4 125.041 34.700 57.351 1.0030.41 H ATOM 1667 CD1 LEU 4 124.005 33.592 57.439 1.00 25.02 H ATOM 1668CD2 LEU 4 124.353 36.059 57.404 1.00 18.34 H ATOM 1669 C LEU 4 127.49932.620 58.945 1.00 47.69 H ATOM 1670 O LEU 4 128.468 33.245 58.524 1.0052.13 H ATOM 1671 N GLN 5 127.500 31.296 59.081 1.00 46.16 H ATOM 1672CA GLN 5 128.669 30.500 58.706 1.00 47.49 H ATOM 1673 CB GLN 5 128.59129.109 59.335 1.00 47.97 H ATOM 1674 CG GLN 5 129.142 29.029 60.747 1.0041.56 H ATOM 1675 CD GLN 5 128.328 28.100 61.633 1.00 64.06 H ATOM 1676OE1 GLN 5 128.475 26.874 61.573 1.00 60.56 H ATOM 1677 NE2 GLN 5 127.45928.681 62.459 1.00 60.41 H ATOM 1678 C GLN 5 128.764 30.379 57.187 1.0036.59 H ATOM 1679 O GLN 5 127.760 30.146 56.519 1.00 31.08 H ATOM 1680 NGLN 6 129.978 30.531 56.659 1.00 35.83 H ATOM 1681 CA GLN 6 130.21430.460 55.220 1.00 33.41 H ATOM 1682 CB GLN 6 130.697 31.824 54.715 1.0015.61 H ATOM 1683 CG GLN 6 130.089 32.252 53.377 1.00 48.11 H ATOM 1684CD GLN 6 130.061 33.762 53.195 1.00 44.23 H ATOM 1685 OE1 GLN 6 129.42934.480 53.971 1.00 44.77 H ATOM 1686 NE2 GLN 6 130.750 34.251 52.1641.00 43.09 H ATOM 1687 C GLN 6 131.230 29.393 54.818 1.00 39.29 H ATOM1688 O GLN 6 131.746 28.655 55.659 1.00 47.11 H ATOM 1689 N SER 7131.492 29.318 53.514 1.00 52.14 H ATOM 1690 CA SER 7 132.464 28.40252.913 1.00 45.28 H ATOM 1691 CB SER 7 133.561 28.037 53.913 1.00 30.02H ATOM 1692 OG SER 7 134.775 27.764 53.240 1.00 36.01 H ATOM 1693 C SER7 131.963 27.125 52.255 1.00 53.29 H ATOM 1694 O SER 7 130.825 26.69152.435 1.00 41.78 H ATOM 1695 N GLY 8 132.873 26.546 51.478 1.00 74.11 HATOM 1696 CA GLY 8 132.656 25.313 50.744 1.00 86.06 H ATOM 1697 C GLY 8133.987 25.088 50.046 1.00 96.74 H ATOM 1698 O GLY 8 135.032 25.20750.691 1.00 99.99 H ATOM 1699 N PRO 9 133.999 24.757 48.739 1.00 99.99 HATOM 1700 CD PRO 9 132.851 24.529 47.850 1.00 99.99 H ATOM 1701 CA PRO 9135.283 24.547 48.047 1.00 95.17 H ATOM 1702 CB PRO 9 134.873 23.92546.702 1.00 99.99 H ATOM 1703 CG PRO 9 133.404 23.557 46.852 1.00 99.98H ATOM 1704 C PRO 9 136.019 25.882 47.872 1.00 83.28 H ATOM 1705 O PRO 9135.387 26.937 47.793 1.00 73.84 H ATOM 1706 N GLU 10 137.349 25.84747.811 1.00 73.05 H ATOM 1707 CA GLU 10 138.109 27.087 47.672 1.00 56.11H ATOM 1708 CB GLU 10 139.003 27.279 48.898 1.00 43.53 H ATOM 1709 CGGLU 10 138.487 26.554 50.136 1.00 30.69 H ATOM 1710 CD GLU 10 137.98827.497 51.217 1.00 42.43 H ATOM 1711 OE1 GLU 10 138.782 28.352 51.6781.00 16.81 H ATOM 1712 OE2 GLU 10 136.803 27.374 51.612 1.00 22.46 HATOM 1713 C GLU 10 138.939 27.223 46.393 1.00 47.28 H ATOM 1714 O GLU 10139.302 28.336 46.017 1.00 48.73 H ATOM 1715 N LEU 11 139.239 26.10645.730 1.00 37.54 H ATOM 1716 CA LEU 11 140.012 26.137 44.486 1.00 30.14H ATOM 1717 CB LEU 11 141.398 25.517 44.676 1.00 20.14 H ATOM 1718 CGLEU 11 142.591 26.256 44.045 1.00 23.62 H ATOM 1719 CD1 LEU 11 143.47725.265 43.309 1.00 18.85 H ATOM 1720 CD2 LEU 11 142.110 27.357 43.1001.00 23.67 H ATOM 1721 C LEU 11 139.306 25.400 43.357 1.00 44.95 H ATOM1722 O LEU 11 138.867 24.257 43.519 1.00 54.63 H ATOM 1723 N VAL 12139.210 26.057 42.207 1.00 41.04 H ATOM 1724 CA VAL 12 138.564 25.46641.042 1.00 44.92 H ATOM 1725 CB VAL 12 137.198 26.116 40.760 1.00 55.40H ATOM 1726 CG1 VAL 12 136.166 25.600 41.736 1.00 60.33 H ATOM 1727 CG2VAL 12 137.324 27.631 40.849 1.00 67.40 H ATOM 1728 C VAL 12 139.42825.687 39.824 1.00 38.99 H ATOM 1729 O VAL 12 140.065 26.727 39.702 1.0023.16 H ATOM 1730 N LYS 13 139.451 24.705 38.928 1.00 56.02 H ATOM 1731CA LYS 13 140.230 24.817 37.701 1.00 61.99 H ATOM 1732 CB LYS 13 140.57723.430 37.154 1.00 47.53 H ATOM 1733 CG LYS 13 142.054 23.066 37.2591.00 29.92 H ATOM 1734 CD LYS 13 142.364 21.831 36.416 1.00 39.21 H ATOM1735 CE LYS 13 143.629 22.007 35.596 1.00 40.05 H ATOM 1736 NZ LYS 13144.804 21.337 36.230 1.00 30.64 H ATOM 1737 C LYS 13 139.365 25.59536.706 1.00 60.22 H ATOM 1738 O LYS 13 138.184 25.287 36.513 1.00 48.99H ATOM 1739 N PRO 14 139.951 26.619 36.066 1.00 55.96 H ATOM 1740 CD PRO14 141.359 27.003 36.236 1.00 55.17 H ATOM 1741 CA PRO 14 139.270 27.47735.091 1.00 63.02 H ATOM 1742 CB PRO 14 140.412 28.234 34.404 1.00 59.73H ATOM 1743 CG PRO 14 141.685 27.633 34.929 1.00 60.43 H ATOM 1744 C PRO14 138.356 26.766 34.096 1.00 61.60 H ATOM 1745 O PRO 14 138.802 25.95733.275 1.00 63.48 H ATOM 1746 N GLY 15 137.069 27.093 34.185 1.00 55.03H ATOM 1747 CA GLY 15 136.077 26.503 33.307 1.00 55.23 H ATOM 1748 C GLY15 134.961 25.815 34.071 1.00 49.69 H ATOM 1749 O GLY 15 133.800 25.86533.670 1.00 45.93 H ATOM 1750 N THR 16 135.304 25.176 35.182 1.00 46.88H ATOM 1751 CA THR 16 134.303 24.480 35.969 1.00 46.72 H ATOM 1752 CBTHR 16 134.946 23.549 36.992 1.00 53.32 H ATOM 1753 OG1 THR 16 135.91424.275 37.758 1.00 63.69 H ATOM 1754 CG2 THR 16 135.615 22.387 36.2861.00 61.76 H ATOM 1755 C THR 16 133.361 25.415 36.700 1.00 42.84 H ATOM1756 O THR 16 133.391 26.626 36.505 1.00 35.33 H ATOM 1757 N SER 17132.535 24.829 37.559 1.00 46.34 H ATOM 1758 CA SER 17 131.543 25.57038.320 1.00 44.29 H ATOM 1759 CB SER 17 130.190 25.458 37.621 1.00 43.09H ATOM 1760 OG SER 17 130.349 25.482 36.211 1.00 37.03 H ATOM 1761 C SER17 131.408 25.051 39.742 1.00 45.39 H ATOM 1762 O SER 17 131.124 23.87239.956 1.00 51.18 H ATOM 1763 N VAL 18 131.599 25.938 40.713 1.00 43.96H ATOM 1764 CA VAL 18 131.483 25.564 42.119 1.00 45.50 H ATOM 1765 CBVAL 18 132.723 25.989 42.932 1.00 47.04 H ATOM 1766 CG1 VAL 18 132.97927.469 42.756 1.00 45.76 H ATOM 1767 CG2 VAL 18 132.513 25.665 44.4001.00 36.17 H ATOM 1768 C VAL 18 130.257 26.218 42.742 1.00 46.62 H ATOM1769 O VAL 18 129.772 27.240 42.258 1.00 24.82 H ATOM 1770 N ARG 19129.758 25.610 43.813 1.00 53.11 H ATOM 1771 CA ARG 19 128.589 26.12244.518 1.00 58.98 H ATOM 1772 CB ARG 19 127.414 25.157 44.354 1.00 48.17H ATOM 1773 CG ARG 19 126.252 25.443 45.280 1.00 32.81 H ATOM 1774 CDARG 19 125.479 24.167 45.605 1.00 61.51 H ATOM 1775 NE ARG 19 126.21223.302 46.530 1.00 70.76 H ATOM 1776 CZ ARG 19 125.661 22.352 47.2831.00 68.17 H ATOM 1777 NH1 ARG 19 124.353 22.124 47.232 1.00 41.10 HATOM 1778 NH2 ARG 19 126.428 21.625 48.090 1.00 47.54 H ATOM 1779 C ARG19 128.912 26.282 46.000 1.00 57.70 H ATOM 1780 O ARG 19 129.050 25.29446.716 1.00 66.45 H ATOM 1781 N ILE 20 129.043 27.523 46.456 1.00 53.34H ATOM 1782 CA ILE 20 129.345 27.780 47.860 1.00 52.34 H ATOM 1783 CBILE 20 130.052 29.137 48.067 1.00 45.51 H ATOM 1784 CG2 ILE 20 131.47429.053 47.565 1.00 47.12 H ATOM 1785 CG1 ILE 20 129.280 30.243 47.3531.00 39.21 H ATOM 1786 CD1 ILE 20 129.929 31.606 47.466 1.00 37.76 HATOM 1787 C ILE 20 128.059 27.800 48.666 1.00 55.83 H ATOM 1788 O ILE 20126.960 27.764 48.113 1.00 48.41 H ATOM 1789 N SER 21 128.208 27.86049.981 1.00 65.06 H ATOM 1790 CA SER 21 127.058 27.892 50.863 1.00 70.97H ATOM 1791 CB SER 21 126.810 26.503 51.468 1.00 72.43 H ATOM 1792 OGSER 21 127.562 26.314 52.654 1.00 60.74 H ATOM 1793 C SER 21 127.26028.917 51.968 1.00 69.87 H ATOM 1794 O SER 21 128.288 29.599 52.026 1.0070.39 H ATOM 1795 N CYS 22 126.262 29.013 52.839 1.00 69.21 H ATOM 1796CA CYS 22 126.263 29.940 53.963 1.00 61.84 H ATOM 1797 C CYS 22 124.99629.635 54.745 1.00 52.88 H ATOM 1798 O CYS 22 123.890 29.845 54.249 1.0052.20 H ATOM 1799 CB CYS 22 126.233 31.379 53.461 1.00 52.16 H ATOM 1800SG CYS 22 125.483 32.535 54.637 1.00 54.59 H ATOM 1801 N GLU 23 125.15429.147 55.968 1.00 40.82 H ATOM 1802 CA GLU 23 123.998 28.783 56.7671.00 43.24 H ATOM 1803 CB GLU 23 124.140 27.339 57.230 1.00 55.82 H ATOM1804 CG GLU 23 125.578 26.915 57.456 1.00 76.19 H ATOM 1805 CD GLU 23125.807 26.370 58.853 1.00 92.47 H ATOM 1806 OE1 GLU 23 124.810 26.02559.525 1.00 98.93 H ATOM 1807 OE2 GLU 23 126.982 26.286 59.278 1.0099.99 H ATOM 1808 C GLU 23 123.723 29.675 57.962 1.00 47.05 H ATOM 1809O GLU 23 124.610 29.940 58.777 1.00 50.01 H ATOM 1810 N ALA 24 122.47230.117 58.060 1.00 39.44 H ATOM 1811 CA ALA 24 122.022 30.980 59.1431.00 43.91 H ATOM 1812 CB ALA 24 120.770 31.729 58.709 1.00 40.39 H ATOM1813 C ALA 24 121.745 30.197 60.429 1.00 49.01 H ATOM 1814 O ALA 24121.532 28.983 60.398 1.00 44.02 H ATOM 1815 N SER 25 121.752 30.90961.555 1.00 60.65 H ATOM 1816 CA SER 25 121.504 30.321 62.872 1.00 64.10H ATOM 1817 CB SER 25 122.557 29.250 63.192 1.00 76.12 H ATOM 1818 OGSER 25 123.782 29.512 62.529 1.00 87.44 H ATOM 1819 C SER 25 121.51431.391 63.966 1.00 53.28 H ATOM 1820 O SER 25 122.520 32.068 64.188 1.0050.41 H ATOM 1821 N GLY 26 120.384 31.533 64.651 1.00 46.94 H ATOM 1822CA GLY 26 120.275 32.520 65.708 1.00 30.92 H ATOM 1823 C GLY 26 119.04433.377 65.502 1.00 32.69 H ATOM 1824 O GLY 26 118.711 34.201 66.349 1.0019.54 H ATOM 1825 N TYR 27 118.372 33.167 64.370 1.00 42.28 H ATOM 1826CA TYR 27 117.163 33.909 64.012 1.00 45.58 H ATOM 1827 CB TYR 27 117.54635.240 63.346 1.00 51.85 H ATOM 1828 CG TYR 27 118.025 35.116 61.9071.00 59.01 H ATOM 1829 CD1 TYR 27 117.155 35.345 60.838 1.00 52.27 HATOM 1830 CE1 TYR 27 117.595 35.261 59.514 1.00 42.40 H ATOM 1831 CD2TYR 27 119.355 34.794 61.614 1.00 52.66 H ATOM 1832 CE2 TYR 27 119.80434.710 60.293 1.00 46.26 H ATOM 1833 CZ TYR 27 118.919 34.947 59.2501.00 50.61 H ATOM 1834 OH TYR 27 119.363 34.893 57.947 1.00 42.85 H ATOM1835 C TYR 27 116.250 33.109 63.072 1.00 48.20 H ATOM 1836 O TYR 27116.553 31.963 62.712 1.00 30.85 H ATOM 1837 N THR 28 115.131 33.72262.684 1.00 47.35 H ATOM 1838 CA THR 28 114.176 33.088 61.776 1.00 41.83H ATOM 1839 CB THR 28 112.786 33.737 61.871 1.00 39.96 H ATOM 1840 OG1THR 28 112.270 33.577 63.197 1.00 50.93 H ATOM 1841 CG2 THR 28 111.83033.084 60.878 1.00 45.16 H ATOM 1842 C THR 28 114.653 33.204 60.328 1.0040.89 H ATOM 1843 O THR 28 114.619 34.280 59.739 1.00 31.24 H ATOM 1844N PHE 29 115.095 32.084 59.766 1.00 41.61 H ATOM 1845 CA PHE 29 115.59132.035 58.399 1.00 41.45 H ATOM 1846 CB PHE 29 115.946 30.593 58.0341.00 30.51 H ATOM 1847 CG PHE 29 116.611 30.449 56.696 1.00 37.17 H ATOM1848 CD1 PHE 29 117.833 31.065 56.439 1.00 30.37 H ATOM 1849 CD2 PHE 29116.032 29.667 55.700 1.00 36.28 H ATOM 1850 CE1 PHE 29 118.469 30.90455.214 1.00 21.32 H ATOM 1851 CE2 PHE 29 116.666 29.500 54.463 1.0041.18 H ATOM 1852 CZ PHE 29 117.889 30.122 54.225 1.00 35.05 H ATOM 1853C PHE 29 114.594 32.572 57.379 1.00 54.24 H ATOM 1854 O PHE 29 114.95233.331 56.480 1.00 66.70 H ATOM 1855 N THR 30 113.342 32.164 57.527 1.0055.99 H ATOM 1856 CA THR 30 112.276 32.557 56.615 1.00 52.36 H ATOM 1857CB THR 30 111.052 31.652 56.824 1.00 53.76 H ATOM 1858 OG1 THR 30110.733 31.583 58.225 1.00 35.56 H ATOM 1859 CG2 THR 30 111.357 30.24456.304 1.00 44.72 H ATOM 1860 C THR 30 111.829 34.016 56.703 1.00 47.50H ATOM 1861 O THR 30 110.914 34.426 55.996 1.00 53.11 H ATOM 1862 N SER31 112.470 34.804 57.554 1.00 46.37 H ATOM 1863 CA SER 31 112.094 36.20757.697 1.00 45.74 H ATOM 1864 CB SER 31 111.832 36.521 59.167 1.00 29.27H ATOM 1865 OG SER 31 110.583 37.159 59.337 1.00 47.69 H ATOM 1866 C SER31 113.143 37.175 57.150 1.00 47.78 H ATOM 1867 O SER 31 113.149 38.35857.509 1.00 46.57 H ATOM 1868 N TYR 32 114.027 36.683 56.286 1.00 31.83H ATOM 1869 CA TYR 32 115.068 37.530 55.720 1.00 30.55 H ATOM 1870 CBTYR 32 116.287 37.556 56.642 1.00 32.62 H ATOM 1871 CG TYR 32 116.07538.248 57.963 1.00 37.51 H ATOM 1872 CD1 TYR 32 115.494 37.579 59.0311.00 33.89 H ATOM 1873 CE1 TYR 32 115.358 38.184 60.266 1.00 35.72 HATOM 1874 CD2 TYR 32 116.510 39.556 58.166 1.00 39.03 H ATOM 1875 CE2TYR 32 116.378 40.173 59.401 1.00 40.85 H ATOM 1876 CZ TYR 32 115.80339.476 60.449 1.00 43.40 H ATOM 1877 OH TYR 32 115.693 40.053 61.6951.00 55.17 H ATOM 1878 C TYR 32 115.526 37.071 54.342 1.00 42.11 H ATOM1879 O TYR 32 115.438 35.889 54.012 1.00 37.33 H ATOM 1880 N TYR 33116.021 38.017 53.545 1.00 52.12 H ATOM 1881 CA TYR 33 116.544 37.72152.210 1.00 50.02 H ATOM 1882 CB TYR 33 116.576 38.960 51.309 1.00 42.85H ATOM 1883 CG TYR 33 115.263 39.601 50.944 1.00 58.80 H ATOM 1884 CD1TYR 33 114.637 39.313 49.730 1.00 66.77 H ATOM 1885 CE1 TYR 33 113.46639.968 49.345 1.00 69.61 H ATOM 1886 CD2 TYR 33 114.683 40.558 51.7711.00 76.77 H ATOM 1887 CE2 TYR 33 113.513 41.220 51.398 1.00 80.20 HATOM 1888 CZ TYR 33 112.909 40.921 50.185 1.00 74.03 H ATOM 1889 OH TYR33 111.746 41.574 49.830 1.00 56.44 H ATOM 1890 C TYR 33 118.000 37.35952.441 1.00 53.48 H ATOM 1891 O TYR 33 118.601 37.825 53.411 1.00 55.64H ATOM 1892 N ILE 34 118.574 36.538 51.570 1.00 39.36 H ATOM 1893 CA ILE34 119.991 36.237 51.705 1.00 36.92 H ATOM 1894 CB ILE 34 120.310 34.72051.687 1.00 41.72 H ATOM 1895 CG2 ILE 34 121.831 34.515 51.776 1.0024.87 H ATOM 1896 CG1 ILE 34 119.647 34.023 52.879 1.00 33.33 H ATOM1897 CD1 ILE 34 120.435 34.137 54.177 1.00 29.68 H ATOM 1898 C ILE 34120.629 36.900 50.490 1.00 33.61 H ATOM 1899 O ILE 34 120.304 36.56849.351 1.00 36.71 H ATOM 1900 N HIS 35 121.506 37.864 50.739 1.00 34.93H ATOM 1901 CA HIS 35 122.185 38.573 49.666 1.00 29.41 H ATOM 1902 CBHIS 35 122.300 40.062 49.990 1.00 22.47 H ATOM 1903 CG HIS 35 120.98340.768 50.047 1.00 28.34 H ATOM 1904 CD2 HIS 35 120.082 40.885 51.0491.00 17.42 H ATOM 1905 ND1 HIS 35 120.446 41.427 48.965 1.00 34.24 HATOM 1906 CE1 HIS 35 119.266 41.924 49.298 1.00 35.85 H ATOM 1907 NE2HIS 35 119.023 41.609 50.556 1.00 28.90 H ATOM 1908 C HIS 35 123.57438.000 49.482 1.00 24.58 H ATOM 1909 O HIS 35 124.156 37.437 50.403 1.0040.74 H ATOM 1910 N TRP 36 124.104 38.149 48.282 1.00 18.85 H ATOM 1911CA TRP 36 125.430 37.664 47.984 1.00 25.49 H ATOM 1912 CB TRP 36 125.33436.410 47.104 1.00 38.00 H ATOM 1913 CG TRP 36 125.226 35.122 47.8821.00 42.56 H ATOM 1914 CD2 TRP 36 126.288 34.433 48.564 1.00 28.82 HATOM 1915 CE2 TRP 36 125.730 33.274 49.146 1.00 18.67 H ATOM 1916 CE3TRP 36 127.658 34.687 48.737 1.00 34.82 H ATOM 1917 CD1 TRP 36 124.09734.368 48.075 1.00 40.81 H ATOM 1918 NE1 TRP 36 124.394 33.257 48.8341.00 30.56 H ATOM 1919 CZ2 TRP 36 126.492 32.368 49.889 1.00 44.00 HATOM 1920 CZ3 TRP 36 128.418 33.786 49.479 1.00 41.71 H ATOM 1921 CH2TRP 36 127.832 32.641 50.045 1.00 49.64 H ATOM 1922 C TRP 36 126.18938.784 47.269 1.00 22.21 H ATOM 1923 O TRP 36 125.658 39.410 46.352 1.0020.33 H ATOM 1924 N VAL 37 127.420 39.044 47.703 1.00 21.22 H ATOM 1925CA VAL 37 128.237 40.085 47.089 1.00 29.63 H ATOM 1926 CB VAL 37 128.15741.414 47.887 1.00 30.69 H ATOM 1927 CG1 VAL 37 126.874 41.453 48.7011.00 7.82 H ATOM 1928 CG2 VAL 37 129.366 41.569 48.780 1.00 16.02 H ATOM1929 C VAL 37 129.693 39.641 46.984 1.00 33.49 H ATOM 1930 O VAL 37130.223 39.017 47.900 1.00 17.14 H ATOM 1931 N LYS 38 130.330 39.97045.862 1.00 43.70 H ATOM 1932 CA LYS 38 131.720 39.591 45.619 1.00 47.72H ATOM 1933 CB LYS 38 131.849 38.932 44.233 1.00 55.60 H ATOM 1934 CGLYS 38 132.592 39.756 43.175 1.00 55.19 H ATOM 1935 CD LYS 38 133.10938.890 42.022 1.00 50.50 H ATOM 1936 CE LYS 38 131.967 38.347 41.1551.00 51.42 H ATOM 1937 NZ LYS 38 132.372 37.162 40.335 1.00 38.13 H ATOM1938 C LYS 38 132.699 40.757 45.732 1.00 48.64 H ATOM 1939 O LYS 38132.467 41.837 45.189 1.00 52.35 H ATOM 1940 N GLN 39 133.798 40.52546.439 1.00 38.36 H ATOM 1941 CA GLN 39 134.819 41.545 46.621 1.00 30.47H ATOM 1942 CB GLN 39 134.969 41.879 48.103 1.00 40.49 H ATOM 1943 CGGLN 39 136.078 42.863 48.409 1.00 23.44 H ATOM 1944 CD GLN 39 135.96543.424 49.803 1.00 27.61 H ATOM 1945 OE1 GLN 39 135.386 44.485 50.0041.00 26.42 H ATOM 1946 NE2 GLN 39 136.517 42.709 50.781 1.00 31.89 HATOM 1947 C GLN 39 136.142 41.029 46.074 1.00 29.22 H ATOM 1948 O GLN 39136.781 40.160 46.672 1.00 19.00 H ATOM 1949 N ARG 40 136.548 41.56044.928 1.00 41.18 H ATOM 1950 CA ARG 40 137.794 41.139 44.310 1.00 48.75H ATOM 1951 CB ARG 40 137.805 41.496 42.819 1.00 47.54 H ATOM 1952 CGARG 40 137.203 40.422 41.923 1.00 39.67 H ATOM 1953 CD ARG 40 137.40940.741 40.448 1.00 63.74 H ATOM 1954 NE ARG 40 138.762 40.415 39.9971.00 66.96 H ATOM 1955 CZ ARG 40 139.215 39.178 39.816 1.00 59.13 H ATOM1956 NH1 ARG 40 138.426 38.137 40.046 1.00 49.49 H ATOM 1957 NH2 ARG 40140.459 38.982 39.400 1.00 64.52 H ATOM 1958 C ARG 40 138.971 41.79145.024 1.00 49.05 H ATOM 1959 O ARG 40 138.955 42.989 45.312 1.00 41.67H ATOM 1960 N PRO 41 140.007 40.996 45.329 1.00 49.95 H ATOM 1961 CD PRO41 140.102 39.557 45.022 1.00 60.26 H ATOM 1962 CA PRO 41 141.200 41.48546.013 1.00 40.75 H ATOM 1963 CB PRO 41 142.268 40.476 45.625 1.00 50.53H ATOM 1964 CG PRO 41 141.516 39.202 45.415 1.00 62.82 H ATOM 1965 C PRO41 141.558 42.888 45.576 1.00 38.02 H ATOM 1966 O PRO 41 141.769 43.14744.392 1.00 47.09 H ATOM 1967 N GLY 42 141.614 43.794 46.542 1.00 36.72H ATOM 1968 CA GLY 42 141.952 45.170 46.241 1.00 31.35 H ATOM 1969 C GLY42 140.726 45.981 45.900 1.00 40.05 H ATOM 1970 O GLY 42 140.468 47.01446.514 1.00 41.40 H ATOM 1971 N GLN 43 139.975 45.502 44.913 1.00 58.75H ATOM 1972 CA GLN 43 138.752 46.163 44.468 1.00 67.06 H ATOM 1973 CBGLN 43 138.192 45.449 43.227 1.00 64.21 H ATOM 1974 CG GLN 43 137.03546.178 42.540 1.00 71.56 H ATOM 1975 CD GLN 43 135.900 45.251 42.1301.00 71.51 H ATOM 1976 OE1 GLN 43 135.330 44.540 42.963 1.00 67.00 HATOM 1977 NE2 GLN 43 135.566 45.255 40.842 1.00 53.00 H ATOM 1978 C GLN43 137.701 46.158 45.581 1.00 68.90 H ATOM 1979 O GLN 43 137.782 45.36646.521 1.00 72.87 H ATOM 1980 N GLY 44 136.723 47.055 45.471 1.00 67.47H ATOM 1981 CA GLY 44 135.661 47.119 46.458 1.00 54.03 H ATOM 1982 C GLY44 134.746 45.927 46.268 1.00 52.55 H ATOM 1983 O GLY 44 135.191 44.88145.794 1.00 65.13 H ATOM 1984 N LEU 45 133.474 46.062 46.623 1.00 38.94H ATOM 1985 CA LEU 45 132.552 44.946 46.456 1.00 50.12 H ATOM 1986 CBLEU 45 132.197 44.339 47.819 1.00 44.25 H ATOM 1987 CG LEU 45 131.30645.109 48.790 1.00 36.87 H ATOM 1988 CD1 LEU 45 131.661 44.692 50.2121.00 34.38 H ATOM 1989 CD2 LEU 45 131.480 46.605 48.597 1.00 31.30 HATOM 1990 C LEU 45 131.283 45.315 45.689 1.00 47.35 H ATOM 1991 O LEU 45130.876 46.475 45.656 1.00 52.97 H ATOM 1992 N GLU 46 130.673 44.31145.066 1.00 38.18 H ATOM 1993 CA GLU 46 129.468 44.497 44.275 1.00 32.36H ATOM 1994 CB GLU 46 129.779 44.312 42.787 1.00 54.58 H ATOM 1995 CGGLU 46 131.106 43.601 42.502 1.00 70.14 H ATOM 1996 CD GLU 46 131.54443.722 41.050 1.00 59.62 H ATOM 1997 OE1 GLU 46 132.769 43.760 40.7961.00 60.49 H ATOM 1998 OE2 GLU 46 130.660 43.778 40.171 1.00 62.64 HATOM 1999 C GLU 46 128.400 43.506 44.686 1.00 33.28 H ATOM 2000 O GLU 46128.697 42.477 45.293 1.00 22.50 H ATOM 2001 N TRP 47 127.157 43.81844.329 1.00 30.17 H ATOM 2002 CA TRP 47 126.010 42.982 44.661 1.00 39.51H ATOM 2003 CB TRP 47 124.794 43.881 44.906 1.00 50.31 H ATOM 2004 CGTRP 47 123.538 43.163 45.304 1.00 46.71 H ATOM 2005 CD2 TRP 47 122.41742.881 44.459 1.00 46.71 H ATOM 2006 CE2 TRP 47 121.440 42.259 45.2621.00 40.21 H ATOM 2007 CE3 TRP 47 122.148 43.096 43.104 1.00 44.16 HATOM 2008 CD1 TRP 47 123.204 42.709 46.547 1.00 44.85 H ATOM 2009 NE1TRP 47 121.942 42.165 46.533 1.00 44.22 H ATOM 2010 CZ2 TRP 47 120.20541.852 44.750 1.00 48.68 H ATOM 2011 CZ3 TRP 47 120.924 42.689 42.5971.00 48.92 H ATOM 2012 CH2 TRP 47 119.969 42.073 43.420 1.00 39.77 HATOM 2013 C TRP 47 125.692 41.936 43.592 1.00 45.09 H ATOM 2014 O TRP 47125.359 42.260 42.449 1.00 45.19 H ATOM 2015 N ILE 48 125.800 40.67143.981 1.00 45.61 H ATOM 2016 CA ILE 48 125.529 39.562 43.080 1.00 37.68H ATOM 2017 CB ILE 48 126.185 38.266 43.601 1.00 25.87 H ATOM 2018 CG2ILE 48 125.479 37.039 43.036 1.00 23.09 H ATOM 2019 CG1 ILE 48 127.65738.249 43.202 1.00 15.27 H ATOM 2020 CD1 ILE 48 128.449 37.133 43.8471.00 20.12 H ATOM 2021 C ILE 48 124.028 39.356 42.936 1.00 35.05 H ATOM2022 O ILE 48 123.461 39.591 41.870 1.00 41.96 H ATOM 2023 N GLY 49123.388 38.918 44.013 1.00 32.61 H ATOM 2024 CA GLY 49 121.953 38.68943.986 1.00 30.75 H ATOM 2025 C GLY 49 121.432 38.298 45.359 1.00 38.37H ATOM 2026 O GLY 49 122.161 38.327 46.349 1.00 41.94 H ATOM 2027 N CYS50 120.163 37.931 45.431 1.00 37.54 H ATOM 2028 CA CYS 50 119.587 37.54046.705 1.00 38.40 H ATOM 2029 CB CYS 50 119.095 38.774 47.462 1.00 51.77H ATOM 2030 SG CYS 50 117.500 39.407 46.877 1.00 50.59 H ATOM 2031 C CYS50 118.438 36.577 46.500 1.00 42.42 H ATOM 2032 O CYS 50 117.835 36.53545.431 1.00 50.09 H ATOM 2033 N ILE 51 118.146 35.804 47.537 1.00 42.92H ATOM 2034 CA ILE 51 117.059 34.837 47.502 1.00 37.44 H ATOM 2035 CBILE 51 117.596 33.380 47.377 1.00 28.69 H ATOM 2036 CG2 ILE 51 118.64833.111 48.440 1.00 29.15 H ATOM 2037 CG1 ILE 51 116.448 32.382 47.5211.00 22.13 H ATOM 2038 CD1 ILE 51 115.840 31.963 46.201 1.00 43.28 HATOM 2039 C ILE 51 116.277 34.989 48.800 1.00 42.00 H ATOM 2040 O ILE 51116.861 35.219 49.868 1.00 34.19 H ATOM 2041 N TYR 52 114.957 34.88648.706 1.00 38.73 H ATOM 2042 CA TYR 52 114.124 35.013 49.888 1.00 34.95H ATOM 2043 CB TYR 52 112.954 35.960 49.643 1.00 26.98 H ATOM 2044 CGTYR 52 112.041 36.049 50.840 1.00 28.39 H ATOM 2045 CD1 TYR 52 112.57136.155 52.132 1.00 31.39 H ATOM 2046 CE1 TYR 52 111.752 36.193 53.2441.00 43.44 H ATOM 2047 CD2 TYR 52 110.660 35.986 50.695 1.00 19.91 HATOM 2048 CE2 TYR 52 109.821 36.022 51.800 1.00 43.98 H ATOM 2049 CZ TYR52 110.376 36.124 53.075 1.00 56.97 H ATOM 2050 OH TYR 52 109.556 36.15354.178 1.00 67.54 H ATOM 2051 C TYR 52 113.578 33.674 50.350 1.00 39.53H ATOM 2052 O TYR 52 112.611 33.161 49.791 1.00 45.44 H ATOM 2053 N PRO53 114.191 33.092 51.386 1.00 46.32 H ATOM 2054 CD PRO 53 115.347 33.61052.137 1.00 58.13 H ATOM 2055 CA PRO 53 113.728 31.803 51.900 1.00 36.81H ATOM 2056 CB PRO 53 114.599 31.566 53.126 1.00 38.59 H ATOM 2057 CGPRO 53 115.817 32.404 52.894 1.00 59.97 H ATOM 2058 C PRO 53 112.27931.971 52.275 1.00 35.25 H ATOM 2059 O PRO 53 111.926 32.927 52.959 1.0037.50 H ATOM 2060 N GLY 54 111.441 31.055 51.815 1.00 43.54 H ATOM 2061CA GLY 54 110.027 31.142 52.120 1.00 53.47 H ATOM 2062 C GLY 54 109.20031.023 50.860 1.00 58.70 H ATOM 2063 O GLY 54 108.726 29.936 50.530 1.0074.82 H ATOM 2064 N ASN 55 109.021 32.132 50.150 1.00 48.62 H ATOM 2065CA ASN 55 108.248 32.116 48.917 1.00 45.89 H ATOM 2066 CB ASN 55 107.60233.482 48.675 1.00 46.66 H ATOM 2067 CG ASN 55 108.344 34.312 47.6581.00 46.59 H ATOM 2068 OD1 ASN 55 109.183 35.138 48.009 1.00 50.93 HATOM 2069 ND2 ASN 55 108.027 34.107 46.384 1.00 65.32 H ATOM 2070 C ASN55 109.177 31.740 47.777 1.00 59.93 H ATOM 2071 O ASN 55 108.738 31.53646.645 1.00 70.19 H ATOM 2072 N VAL 56 110.466 31.644 48.110 1.00 68.48H ATOM 2073 CA VAL 56 111.541 31.269 47.182 1.00 68.32 H ATOM 2074 CBVAL 56 111.181 29.960 46.416 1.00 73.69 H ATOM 2075 CG1 VAL 56 110.73930.271 44.989 1.00 75.39 H ATOM 2076 CG2 VAL 56 112.377 29.021 46.4151.00 57.36 H ATOM 2077 C VAL 56 111.958 32.352 46.182 1.00 64.26 H ATOM2078 O VAL 56 112.859 32.142 45.366 1.00 50.69 H ATOM 2079 N ASN 57111.307 33.511 46.258 1.00 70.60 H ATOM 2080 CA ASN 57 111.610 34.62245.366 1.00 62.78 H ATOM 2081 CB ASN 57 110.996 35.913 45.898 1.00 76.22H ATOM 2082 CG ASN 57 109.868 36.422 45.032 1.00 82.20 H ATOM 2083 OD1ASN 57 109.018 37.191 45.486 1.00 72.33 H ATOM 2084 ND2 ASN 57 109.84935.989 43.772 1.00 72.74 H ATOM 2085 C ASN 57 113.110 34.795 45.242 1.0063.32 H ATOM 2086 O ASN 57 113.857 34.515 46.181 1.00 68.16 H ATOM 2087N THR 58 113.553 35.267 44.084 1.00 54.53 H ATOM 2088 CA THR 58 114.97435.465 43.859 1.00 52.32 H ATOM 2089 CB THR 58 115.597 34.191 43.2791.00 48.24 H ATOM 2090 OG1 THR 58 116.806 34.523 42.594 1.00 45.77 HATOM 2091 CG2 THR 58 114.628 33.516 42.314 1.00 53.99 H ATOM 2092 C THR58 115.243 36.641 42.921 1.00 52.31 H ATOM 2093 O THR 58 114.436 36.93542.039 1.00 59.83 H ATOM 2094 N ASN 59 116.375 37.313 43.117 1.00 40.59H ATOM 2095 CA ASN 59 116.730 38.462 42.290 1.00 38.18 H ATOM 2096 CBASN 59 116.330 39.758 42.998 1.00 53.76 H ATOM 2097 CG ASN 59 114.92840.217 42.632 1.00 53.44 H ATOM 2098 OD1 ASN 59 114.712 40.809 41.5721.00 58.59 H ATOM 2099 ND2 ASN 59 113.970 39.951 43.512 1.00 44.63 HATOM 2100 C ASN 59 118.217 38.505 41.970 1.00 30.27 H ATOM 2101 O ASN 59119.049 38.423 42.873 1.00 35.55 H ATOM 2102 N TYR 60 118.544 38.66140.687 1.00 19.72 H ATOM 2103 CA TYR 60 119.935 38.710 40.243 1.00 29.52H ATOM 2104 CB TYR 60 120.213 37.596 39.227 1.00 27.47 H ATOM 2105 CGTYR 60 119.809 36.215 39.675 1.00 25.29 H ATOM 2106 CD1 TYR 60 120.76235.233 39.929 1.00 18.79 H ATOM 2107 CE1 TYR 60 120.382 33.951 40.3251.00 40.31 H ATOM 2108 CD2 TYR 60 118.468 35.883 39.825 1.00 40.64 HATOM 2109 CE2 TYR 60 118.077 34.611 40.217 1.00 47.25 H ATOM 2110 CZ TYR60 119.033 33.647 40.469 1.00 51.22 H ATOM 2111 OH TYR 60 118.621 32.39140.883 1.00 56.18 H ATOM 2112 C TYR 60 120.367 40.034 39.618 1.00 25.63H ATOM 2113 O TYR 60 119.574 40.746 39.008 1.00 17.78 H ATOM 2114 N ASN61 121.645 40.354 39.789 1.00 31.77 H ATOM 2115 CA ASN 61 122.224 41.55139.213 1.00 30.42 H ATOM 2116 CB ASN 61 123.542 41.891 39.907 1.00 26.96H ATOM 2117 CG ASN 61 124.176 43.155 39.367 1.00 28.98 H ATOM 2118 OD1ASN 61 124.721 43.964 40.120 1.00 39.03 H ATOM 2119 ND2 ASN 61 124.10543.335 38.052 1.00 39.88 H ATOM 2120 C ASN 61 122.480 41.102 37.787 1.0042.86 H ATOM 2121 O ASN 61 123.062 40.041 37.571 1.00 56.00 H ATOM 2122N GLU 62 122.045 41.889 36.812 1.00 46.51 H ATOM 2123 CA GLU 62 122.22441.498 35.424 1.00 52.07 H ATOM 2124 CB GLU 62 121.511 42.490 34.5041.00 57.39 H ATOM 2125 CG GLU 62 120.513 41.839 33.531 1.00 76.62 H ATOM2126 CD GLU 62 119.280 41.242 34.214 1.00 81.14 H ATOM 2127 OE1 GLU 62119.442 40.484 35.197 1.00 79.43 H ATOM 2128 OE2 GLU 62 118.148 41.52833.759 1.00 70.90 H ATOM 2129 C GLU 62 123.678 41.327 35.000 1.00 53.65H ATOM 2130 O GLU 62 123.953 40.991 33.847 1.00 75.05 H ATOM 2131 N LYS63 124.610 41.543 35.922 1.00 36.05 H ATOM 2132 CA LYS 63 126.025 41.37935.604 1.00 35.02 H ATOM 2133 CB LYS 63 126.862 42.421 36.353 1.00 26.34H ATOM 2134 CG LYS 63 127.215 43.653 35.532 1.00 49.15 H ATOM 2135 CDLYS 63 126.773 44.948 36.217 1.00 45.62 H ATOM 2136 CE LYS 63 127.93845.619 36.920 1.00 40.94 H ATOM 2137 NZ LYS 63 128.652 44.625 37.7721.00 39.59 H ATOM 2138 C LYS 63 126.506 39.969 35.961 1.00 40.58 H ATOM2139 O LYS 63 127.618 39.576 35.611 1.00 45.93 H ATOM 2140 N PHE 64125.657 39.214 36.658 1.00 47.98 H ATOM 2141 CA PHE 64 125.975 37.84937.073 1.00 42.86 H ATOM 2142 CB PHE 64 126.263 37.789 38.579 1.00 42.85H ATOM 2143 CG PHE 64 127.177 38.872 39.068 1.00 44.03 H ATOM 2144 CD1PHE 64 128.538 38.831 38.791 1.00 50.59 H ATOM 2145 CD2 PHE 64 126.68039.929 39.817 1.00 38.08 H ATOM 2146 CE1 PHE 64 129.388 39.830 39.2551.00 45.27 H ATOM 2147 CE2 PHE 64 127.524 40.933 40.284 1.00 43.09 HATOM 2148 CZ PHE 64 128.877 40.883 40.004 1.00 31.11 H ATOM 2149 C PHE64 124.823 36.908 36.768 1.00 43.11 H ATOM 2150 O PHE 64 124.560 35.98837.535 1.00 47.33 H ATOM 2151 N LYS 65 124.148 37.128 35.644 1.00 55.21H ATOM 2152 CA LYS 65 123.005 36.302 35.259 1.00 59.07 H ATOM 2153 CBLYS 65 122.383 36.806 33.949 1.00 45.97 H ATOM 2154 CG LYS 65 123.14637.914 33.251 1.00 49.55 H ATOM 2155 CD LYS 65 124.165 37.363 32.2701.00 46.70 H ATOM 2156 CE LYS 65 125.116 38.461 31.811 1.00 50.30 H ATOM2157 NZ LYS 65 125.548 38.271 30.398 1.00 58.93 H ATOM 2158 C LYS 65123.279 34.802 35.118 1.00 67.16 H ATOM 2159 O LYS 65 122.598 33.98435.741 1.00 70.12 H ATOM 2160 N ASP 66 124.267 34.440 34.304 1.00 69.41H ATOM 2161 CA ASP 66 124.579 33.031 34.073 1.00 66.83 H ATOM 2162 CBASP 66 125.508 32.898 32.872 1.00 66.07 H ATOM 2163 CG ASP 66 124.74732.712 31.580 1.00 66.45 H ATOM 2164 OD1 ASP 66 124.869 31.628 30.9731.00 66.35 H ATOM 2165 OD2 ASP 66 124.023 33.649 31.177 1.00 50.12 HATOM 2166 C ASP 66 125.168 32.265 35.251 1.00 70.25 H ATOM 2167 O ASP 66124.514 31.365 35.792 1.00 72.29 H ATOM 2168 N LYS 67 126.399 32.60535.637 1.00 62.38 H ATOM 2169 CA LYS 67 127.071 31.934 36.750 1.00 49.02H ATOM 2170 CB LYS 67 128.374 32.653 37.115 1.00 25.39 H ATOM 2171 CGLYS 67 128.541 34.034 36.489 1.00 48.63 H ATOM 2172 CD LYS 67 129.95834.579 36.675 1.00 21.16 H ATOM 2173 CE LYS 67 130.912 34.018 35.6241.00 43.17 H ATOM 2174 NZ LYS 67 132.251 33.618 36.179 1.00 34.20 H ATOM2175 C LYS 67 126.178 31.887 37.977 1.00 59.48 H ATOM 2176 O LYS 67125.721 30.816 38.390 1.00 51.12 H ATOM 2177 N ALA 68 125.929 33.06638.545 1.00 67.14 H ATOM 2178 CA ALA 68 125.104 33.220 39.741 1.00 54.74H ATOM 2179 CB ALA 68 125.017 34.688 40.108 1.00 54.84 H ATOM 2180 C ALA68 123.701 32.631 39.631 1.00 49.40 H ATOM 2181 O ALA 68 122.851 33.13638.896 1.00 58.08 H ATOM 2182 N THR 69 123.473 31.564 40.387 1.00 32.05H ATOM 2183 CA THR 69 122.192 30.874 40.429 1.00 37.56 H ATOM 2184 CBTHR 69 122.284 29.541 39.655 1.00 35.94 H ATOM 2185 OG1 THR 69 122.39229.821 38.253 1.00 40.10 H ATOM 2186 CG2 THR 69 121.065 28.676 39.9081.00 26.92 H ATOM 2187 C THR 69 121.915 30.626 41.914 1.00 43.54 H ATOM2188 O THR 69 122.595 29.823 42.550 1.00 40.52 H ATOM 2189 N LEU 70120.924 31.319 42.469 1.00 45.97 H ATOM 2190 CA LEU 70 120.622 31.18943.893 1.00 46.62 H ATOM 2191 CB LEU 70 120.166 32.536 44.445 1.00 47.28H ATOM 2192 CG LEU 70 121.305 33.331 45.091 1.00 55.81 H ATOM 2193 CD1LEU 70 121.133 34.805 44.767 1.00 43.73 H ATOM 2194 CD2 LEU 70 121.32233.094 46.611 1.00 43.94 H ATOM 2195 C LEU 70 119.636 30.107 44.312 1.0043.94 H ATOM 2196 O LEU 70 118.496 30.070 43.849 1.00 42.32 H ATOM 2197N ILE 71 120.103 29.247 45.218 1.00 44.21 H ATOM 2198 CA ILE 71 119.33328.125 45.755 1.00 49.89 H ATOM 2199 CB ILE 71 120.026 26.770 45.4301.00 51.60 H ATOM 2200 CG2 ILE 71 119.259 25.616 46.072 1.00 29.06 HATOM 2201 CG1 ILE 71 120.107 26.573 43.911 1.00 54.43 H ATOM 2202 CD1ILE 71 121.184 27.402 43.222 1.00 43.28 H ATOM 2203 C ILE 71 119.20328.255 47.278 1.00 52.79 H ATOM 2204 O ILE 71 120.103 28.779 47.939 1.0062.18 H ATOM 2205 N VAL 72 118.093 27.768 47.830 1.00 50.92 H ATOM 2206CA VAL 72 117.837 27.843 49.269 1.00 52.77 H ATOM 2207 CB VAL 72 116.55128.677 49.549 1.00 47.96 H ATOM 2208 CG1 VAL 72 115.313 27.828 49.3171.00 44.08 H ATOM 2209 CG2 VAL 72 116.569 29.224 50.967 1.00 43.63 HATOM 2210 C VAL 72 117.687 26.437 49.861 1.00 56.26 H ATOM 2211 O VAL 72117.627 25.457 49.123 1.00 65.48 H ATOM 2212 N ASP 73 117.638 26.34451.191 1.00 56.09 H ATOM 2213 CA ASP 73 117.492 25.064 51.891 1.00 50.35H ATOM 2214 CB ASP 73 118.848 24.365 51.995 1.00 53.65 H ATOM 2215 CGASP 73 118.721 22.907 52.384 1.00 57.99 H ATOM 2216 OD1 ASP 73 118.11922.616 53.443 1.00 57.92 H ATOM 2217 OD2 ASP 73 119.229 22.055 51.6251.00 61.79 H ATOM 2218 C ASP 73 116.932 25.313 53.290 1.00 50.51 H ATOM2219 O ASP 73 117.664 25.288 54.280 1.00 43.10 H ATOM 2220 N THR 74115.624 25.535 53.352 1.00 56.54 H ATOM 2221 CA THR 74 114.913 25.83654.593 1.00 66.24 H ATOM 2222 CB THR 74 113.421 26.020 54.296 1.00 71.27H ATOM 2223 OG1 THR 74 113.183 25.793 52.901 1.00 71.81 H ATOM 2224 CG2THR 74 112.979 27.430 54.659 1.00 81.34 H ATOM 2225 C THR 74 115.05424.876 55.785 1.00 71.66 H ATOM 2226 O THR 74 114.662 25.220 56.902 1.0067.59 H ATOM 2227 N SER 75 115.610 23.689 55.559 1.00 75.40 H ATOM 2228CA SER 75 115.775 22.712 56.634 1.00 69.25 H ATOM 2229 CB SER 75 115.83321.292 56.063 1.00 76.48 H ATOM 2230 OG SER 75 116.615 20.445 56.8911.00 75.33 H ATOM 2231 C SER 75 117.024 22.961 57.474 1.00 60.40 H ATOM2232 O SER 75 116.949 23.066 58.703 1.00 51.38 H ATOM 2233 N SER 76118.171 23.040 56.807 1.00 45.77 H ATOM 2234 CA SER 76 119.435 23.28257.498 1.00 44.97 H ATOM 2235 CB SER 76 120.585 22.601 56.749 1.00 51.28H ATOM 2236 OG SER 76 120.106 21.807 55.676 1.00 43.07 H ATOM 2237 C SER76 119.691 24.781 57.589 1.00 34.83 H ATOM 2238 O SER 76 120.755 25.22158.008 1.00 28.11 H ATOM 2239 N ASN 77 118.697 25.563 57.194 1.00 46.96H ATOM 2240 CA ASN 77 118.826 27.009 57.222 1.00 38.99 H ATOM 2241 CBASN 77 118.847 27.513 58.665 1.00 41.25 H ATOM 2242 CG ASN 77 117.45927.785 59.206 1.00 39.52 H ATOM 2243 OD1 ASN 77 116.470 27.731 58.4681.00 40.29 H ATOM 2244 ND2 ASN 77 117.377 28.082 60.496 1.00 23.39 HATOM 2245 C ASN 77 120.111 27.395 56.515 1.00 34.49 H ATOM 2246 O ASN 77120.971 28.067 57.077 1.00 45.69 H ATOM 2247 N THR 78 120.240 26.94455.275 1.00 39.07 H ATOM 2248 CA THR 78 121.417 27.244 54.481 1.00 43.99H ATOM 2249 CB THR 78 122.340 26.001 54.336 1.00 47.54 H ATOM 2250 OG1THR 78 121.955 25.239 53.189 1.00 23.72 H ATOM 2251 CG2 THR 78 122.24425.122 55.568 1.00 19.39 H ATOM 2252 C THR 78 121.030 27.761 53.100 1.0035.58 H ATOM 2253 O THR 78 120.107 27.255 52.465 1.00 41.21 H ATOM 2254N ALA 79 121.738 28.791 52.658 1.00 32.09 H ATOM 2255 CA ALA 79 121.49829.399 51.361 1.00 45.71 H ATOM 2256 CB ALA 79 121.265 30.903 51.5201.00 20.04 H ATOM 2257 C ALA 79 122.734 29.148 50.509 1.00 54.97 H ATOM2258 O ALA 79 123.854 29.447 50.927 1.00 71.91 H ATOM 2259 N TYR 80122.550 28.589 49.321 1.00 37.02 H ATOM 2260 CA TYR 80 123.697 28.32948.480 1.00 32.51 H ATOM 2261 CB TYR 80 123.635 26.921 47.920 1.00 27.41H ATOM 2262 CG TYR 80 123.465 25.846 48.950 1.00 28.36 H ATOM 2263 CD1TYR 80 124.539 25.049 49.332 1.00 30.12 H ATOM 2264 CE1 TYR 80 124.37124.000 50.232 1.00 47.45 H ATOM 2265 CD2 TYR 80 122.212 25.575 49.4951.00 29.76 H ATOM 2266 CE2 TYR 80 122.028 24.529 50.394 1.00 40.26 HATOM 2267 CZ TYR 80 123.111 23.742 50.759 1.00 45.55 H ATOM 2268 OH TYR80 122.933 22.695 51.643 1.00 45.67 H ATOM 2269 C TYR 80 123.819 29.30747.327 1.00 47.49 H ATOM 2270 O TYR 80 122.870 30.010 46.974 1.00 54.32H ATOM 2271 N MET 81 125.012 29.328 46.744 1.00 58.24 H ATOM 2272 CA MET81 125.340 30.175 45.609 1.00 51.28 H ATOM 2273 CB MET 81 126.117 31.40146.073 1.00 47.13 H ATOM 2274 CG MET 81 127.250 31.794 45.151 1.00 49.47H ATOM 2275 SD MET 81 126.890 33.310 44.268 1.00 52.80 H ATOM 2276 CEMET 81 127.508 34.508 45.421 1.00 52.92 H ATOM 2277 C MET 81 126.22029.327 44.699 1.00 51.47 H ATOM 2278 O MET 81 127.342 28.976 45.065 1.0048.72 H ATOM 2279 N GLN 82 125.707 28.981 43.525 1.00 46.87 H ATOM 2280CA GLN 82 126.476 28.175 42.595 1.00 45.38 H ATOM 2281 CB GLN 82 125.58927.113 41.943 1.00 71.53 H ATOM 2282 CG GLN 82 125.978 26.739 40.5111.00 89.74 H ATOM 2283 CD GLN 82 127.278 25.955 40.433 1.00 96.73 H ATOM2284 OE1 GLN 82 127.925 25.699 41.448 1.00 98.73 H ATOM 2285 NE2 GLN 82127.665 25.572 39.220 1.00 94.58 H ATOM 2286 C GLN 82 127.075 29.06541.530 1.00 44.59 H ATOM 2287 O GLN 82 126.366 29.787 40.833 1.00 42.20H ATOM 2288 N LEU 83 128.394 29.014 41.422 1.00 49.17 H ATOM 2289 CA LEU83 129.114 29.797 40.434 1.00 46.27 H ATOM 2290 CB LEU 83 130.369 30.39841.067 1.00 32.33 H ATOM 2291 CG LEU 83 130.033 31.416 42.160 1.00 31.17H ATOM 2292 CD1 LEU 83 131.119 31.455 43.252 1.00 6.90 H ATOM 2293 CD2LEU 83 129.865 32.766 41.496 1.00 18.19 H ATOM 2294 C LEU 83 129.47128.845 39.298 1.00 45.11 H ATOM 2295 O LEU 83 130.201 27.870 39.496 1.0036.84 H ATOM 2296 N SER 84 128.925 29.118 38.117 1.00 44.08 H ATOM 2297CA SER 84 129.160 28.280 36.948 1.00 46.97 H ATOM 2298 CB SER 84 127.82927.869 36.323 1.00 54.37 H ATOM 2299 OG SER 84 127.604 28.576 35.1181.00 64.19 H ATOM 2300 C SER 84 130.037 28.942 35.896 1.00 49.55 H ATOM2301 O SER 84 129.905 30.137 35.617 1.00 47.92 H ATOM 2302 N ARG 85130.925 28.146 35.306 1.00 48.23 H ATOM 2303 CA ARG 85 131.842 28.63734.294 1.00 41.86 H ATOM 2304 CB ARG 85 131.057 29.141 33.082 1.00 46.21H ATOM 2305 CG ARG 85 130.474 28.012 32.236 1.00 41.59 H ATOM 2306 CDARG 85 129.125 28.393 31.630 1.00 68.47 H ATOM 2307 NE ARG 85 129.17428.406 30.170 1.00 68.50 H ATOM 2308 CZ ARG 85 128.142 28.126 29.3811.00 60.66 H ATOM 2309 NH1 ARG 85 126.962 27.808 29.906 1.00 56.39 HATOM 2310 NH2 ARG 85 128.293 28.166 28.065 1.00 55.47 H ATOM 2311 C ARG85 132.670 29.752 34.912 1.00 40.27 H ATOM 2312 O ARG 85 132.430 30.93934.669 1.00 37.17 H ATOM 2313 N MET 86 133.645 29.350 35.722 1.00 35.41H ATOM 2314 CA MET 86 134.518 30.288 36.412 1.00 32.72 H ATOM 2315 CBMET 86 134.974 29.684 37.733 1.00 38.34 H ATOM 2316 CG MET 86 134.09128.551 38.215 1.00 45.32 H ATOM 2317 SD MET 86 133.310 28.856 39.8041.00 63.97 H ATOM 2318 CE MET 86 133.680 30.599 40.068 1.00 45.50 H ATOM2319 C MET 86 135.733 30.691 35.592 1.00 33.88 H ATOM 2320 O MET 86136.097 30.018 34.627 1.00 28.01 H ATOM 2321 N THR 87 136.357 31.79435.996 1.00 37.82 H ATOM 2322 CA THR 87 137.525 32.330 35.310 1.00 39.63H ATOM 2323 CB THR 87 137.081 33.070 34.035 1.00 31.11 H ATOM 2324 OG1THR 87 136.455 32.132 33.153 1.00 44.08 H ATOM 2325 CG2 THR 87 138.26333.706 33.324 1.00 41.18 H ATOM 2326 C THR 87 138.299 33.282 36.232 1.0049.08 H ATOM 2327 O THR 87 137.797 33.684 37.278 1.00 56.80 H ATOM 2328N SER 88 139.523 33.634 35.845 1.00 59.16 H ATOM 2329 CA SER 88 140.36234.539 36.633 1.00 63.45 H ATOM 2330 CB SER 88 141.693 34.780 35.9111.00 64.98 H ATOM 2331 OG SER 88 142.614 33.729 36.165 1.00 44.05 H ATOM2332 C SER 88 139.672 35.878 36.897 1.00 61.47 H ATOM 2333 O SER 88140.291 36.838 37.356 1.00 63.13 H ATOM 2334 N GLU 89 138.384 35.92836.586 1.00 61.96 H ATOM 2335 CA GLU 89 137.567 37.114 36.791 1.00 60.40H ATOM 2336 CB GLU 89 136.888 37.517 35.483 1.00 58.39 H ATOM 2337 CGGLU 89 136.045 36.410 34.861 1.00 65.30 H ATOM 2338 CD GLU 89 134.60736.832 34.614 1.00 74.02 H ATOM 2339 OE1 GLU 89 134.227 36.969 33.4301.00 59.34 H ATOM 2340 OE2 GLU 89 133.858 37.028 35.604 1.00 71.55 HATOM 2341 C GLU 89 136.518 36.725 37.822 1.00 58.77 H ATOM 2342 O GLU 89135.492 37.388 37.971 1.00 54.85 H ATOM 2343 N ASP 90 136.788 35.62438.514 1.00 59.98 H ATOM 2344 CA ASP 90 135.898 35.102 39.544 1.00 62.69H ATOM 2345 CB ASP 90 135.475 33.664 39.211 1.00 63.78 H ATOM 2346 CGASP 90 134.268 33.601 38.293 1.00 63.07 H ATOM 2347 OD1 ASP 90 133.72832.488 38.109 1.00 51.98 H ATOM 2348 OD2 ASP 90 133.864 34.653 37.7571.00 64.25 H ATOM 2349 C ASP 90 136.653 35.105 40.867 1.00 58.67 H ATOM2350 O ASP 90 136.052 35.098 41.939 1.00 60.42 H ATOM 2351 N SER 91137.979 35.102 40.783 1.00 57.82 H ATOM 2352 CA SER 91 138.806 35.11341.978 1.00 51.69 H ATOM 2353 CB SER 91 140.285 35.096 41.597 1.00 44.52H ATOM 2354 OG SER 91 140.598 33.935 40.846 1.00 46.65 H ATOM 2355 C SER91 138.487 36.362 42.787 1.00 53.31 H ATOM 2356 O SER 91 138.881 37.47142.427 1.00 45.71 H ATOM 2357 N ALA 92 137.755 36.167 43.877 1.00 53.93H ATOM 2358 CA ALA 92 137.356 37.257 44.755 1.00 50.72 H ATOM 2359 CBALA 92 136.133 37.967 44.177 1.00 48.61 H ATOM 2360 C ALA 92 137.04536.691 46.143 1.00 46.86 H ATOM 2361 O ALA 92 137.638 35.690 46.545 1.0049.03 H ATOM 2362 N VAL 93 136.116 37.313 46.868 1.00 46.02 H ATOM 2363CA VAL 93 135.775 36.842 48.207 1.00 44.17 H ATOM 2364 CB VAL 93 136.11637.909 49.272 1.00 39.83 H ATOM 2365 CG1 VAL 93 136.006 37.299 50.6631.00 31.85 H ATOM 2366 CG2 VAL 93 137.523 38.450 49.049 1.00 37.20 HATOM 2367 C VAL 93 134.330 36.371 48.439 1.00 45.91 H ATOM 2368 O VAL 93134.092 35.525 49.301 1.00 48.37 H ATOM 2369 N TYR 94 133.373 36.91647.691 1.00 38.60 H ATOM 2370 CA TYR 94 131.963 36.524 47.825 1.00 49.33H ATOM 2371 CB TYR 94 131.706 35.189 47.125 1.00 40.10 H ATOM 2372 CGTYR 94 132.160 35.137 45.692 1.00 31.43 H ATOM 2373 CD1 TYR 94 131.37235.660 44.675 1.00 29.40 H ATOM 2374 CE1 TYR 94 131.758 35.556 43.3441.00 41.76 H ATOM 2375 CD2 TYR 94 133.355 34.512 45.346 1.00 33.02 HATOM 2376 CE2 TYR 94 133.750 34.402 44.025 1.00 31.73 H ATOM 2377 CZ TYR94 132.949 34.922 43.028 1.00 46.94 H ATOM 2378 OH TYR 94 133.336 34.79641.711 1.00 62.64 H ATOM 2379 C TYR 94 131.450 36.407 49.267 1.00 50.08H ATOM 2380 O TYR 94 131.708 35.413 49.953 1.00 34.70 H ATOM 2381 N PHE95 130.688 37.408 49.703 1.00 50.03 H ATOM 2382 CA PHE 95 130.140 37.43151.051 1.00 34.71 H ATOM 2383 CB PHE 95 130.292 38.814 51.671 1.00 32.84H ATOM 2384 CG PHE 95 131.646 39.077 52.243 1.00 19.95 H ATOM 2385 CD1PHE 95 131.966 38.638 53.516 1.00 6.35 H ATOM 2386 CD2 PHE 95 132.60039.780 51.508 1.00 14.02 H ATOM 2387 CE1 PHE 95 133.219 38.892 54.0571.00 29.11 H ATOM 2388 CE2 PHE 95 133.849 40.037 52.041 1.00 21.81 HATOM 2389 CZ PHE 95 134.162 39.592 53.321 1.00 20.98 H ATOM 2390 C PHE95 128.678 37.082 51.077 1.00 37.58 H ATOM 2391 O PHE 95 127.941 37.37850.146 1.00 40.29 H ATOM 2392 N CYS 96 128.272 36.455 52.168 1.00 47.35H ATOM 2393 CA CYS 96 126.888 36.080 52.375 1.00 48.56 H ATOM 2394 C CYS96 126.368 37.062 53.415 1.00 47.93 H ATOM 2395 O CYS 96 127.035 37.32654.420 1.00 49.37 H ATOM 2396 CB CYS 96 126.796 34.652 52.911 1.00 45.82H ATOM 2397 SG CYS 96 125.140 34.190 53.511 1.00 75.54 H ATOM 2398 N THR97 125.188 37.614 53.168 1.00 37.05 H ATOM 2399 CA THR 97 124.595 38.57554.086 1.00 35.84 H ATOM 2400 CB THR 97 125.127 40.008 53.807 1.00 33.55H ATOM 2401 OG1 THR 97 124.832 40.851 54.922 1.00 24.79 H ATOM 2402 CG2THR 97 124.517 40.584 52.542 1.00 23.01 H ATOM 2403 C THR 97 123.08538.518 53.931 1.00 34.60 H ATOM 2404 O THR 97 122.584 37.860 53.026 1.0034.26 H ATOM 2405 N ARG 98 122.356 39.192 54.814 1.00 46.98 H ATOM 2406CA ARG 98 120.896 39.158 54.752 1.00 49.01 H ATOM 2407 CB ARG 98 120.35238.157 55.795 1.00 38.44 H ATOM 2408 CG ARG 98 119.596 38.781 56.9631.00 40.81 H ATOM 2409 CD ARG 98 120.319 38.594 58.296 1.00 27.38 H ATOM2410 NE ARG 98 119.849 39.546 59.307 1.00 53.08 H ATOM 2411 CZ ARG 98119.540 39.230 60.563 1.00 40.53 H ATOM 2412 NH1 ARG 98 119.651 37.97760.980 1.00 58.43 H ATOM 2413 NH2 ARG 98 119.113 40.167 61.403 1.0033.33 H ATOM 2414 C ARG 98 120.229 40.507 54.938 1.00 39.44 H ATOM 2415O ARG 98 120.740 41.377 55.637 1.00 41.38 H ATOM 2416 N SER 99 119.07940.666 54.296 1.00 35.04 H ATOM 2417 CA SER 99 118.299 41.889 54.3881.00 24.81 H ATOM 2418 CB SER 99 117.920 42.396 53.004 1.00 14.04 H ATOM2419 OG SER 99 117.671 43.780 53.039 1.00 29.22 H ATOM 2420 C SER 99117.050 41.455 55.106 1.00 28.87 H ATOM 2421 O SER 99 116.660 40.29455.009 1.00 36.12 H ATOM 2422 N HIS 100 116.418 42.367 55.829 1.00 34.54H ATOM 2423 CA HIS 100 115.209 41.998 56.541 1.00 40.60 H ATOM 2424 CBHIS 100 114.976 42.943 57.714 1.00 35.00 H ATOM 2425 CG HIS 100 113.73142.641 58.492 1.00 48.41 H ATOM 2426 CD2 HIS 100 113.141 41.463 58.8121.00 45.56 H ATOM 2427 ND1 HIS 100 112.947 43.625 59.057 1.00 32.49 HATOM 2428 CE1 HIS 100 111.930 43.069 59.687 1.00 29.00 H ATOM 2429 NE2HIS 100 112.023 41.759 59.557 1.00 33.71 H ATOM 2430 C HIS 100 114.03742.072 55.584 1.00 41.32 H ATOM 2431 O HIS 100 113.824 43.110 54.9641.00 48.72 H ATOM 2432 N TYR 101 113.295 40.976 55.438 1.00 35.01 H ATOM2433 CA TYR 101 112.136 41.001 54.551 1.00 39.86 H ATOM 2434 CB TYR 101111.509 39.606 54.408 1.00 26.52 H ATOM 2435 CG TYR 101 110.507 39.49653.267 1.00 26.05 H ATOM 2436 CD1 TYR 101 110.933 39.404 51.945 1.0040.86 H ATOM 2437 CE1 TYR 101 110.024 39.324 50.885 1.00 27.58 H ATOM2438 CD2 TYR 101 109.135 39.502 53.507 1.00 33.96 H ATOM 2439 CE2 TYR101 108.216 39.422 52.450 1.00 40.43 H ATOM 2440 CZ TYR 101 108.67339.336 51.145 1.00 27.74 H ATOM 2441 OH TYR 101 107.779 39.275 50.1001.00 40.21 H ATOM 2442 C TYR 101 111.167 41.950 55.247 1.00 55.50 H ATOM2443 O TYR 101 111.013 41.892 56.470 1.00 73.70 H ATOM 2444 N GLY 102110.525 42.828 54.482 1.00 45.70 H ATOM 2445 CA GLY 102 109.611 43.79055.079 1.00 43.01 H ATOM 2446 C GLY 102 110.364 45.094 55.237 1.00 39.11H ATOM 2447 O GLY 102 110.169 46.028 54.464 1.00 47.90 H ATOM 2448 N LEU103 111.229 45.155 56.243 1.00 31.80 H ATOM 2449 CA LEU 103 112.06246.331 56.475 1.00 34.98 H ATOM 2450 CB LEU 103 112.270 46.554 57.9751.00 47.24 H ATOM 2451 CG LEU 103 111.258 47.406 58.750 1.00 56.12 HATOM 2452 CD1 LEU 103 109.959 47.593 57.956 1.00 31.67 H ATOM 2453 CD2LEU 103 110.993 46.730 60.092 1.00 44.52 H ATOM 2454 C LEU 103 113.39446.019 55.807 1.00 31.85 H ATOM 2455 O LEU 103 114.392 45.736 56.4711.00 32.76 H ATOM 2456 N ASP 104 113.368 46.045 54.477 1.00 33.42 H ATOM2457 CA ASP 104 114.512 45.757 53.625 1.00 20.79 H ATOM 2458 CB ASP 104113.992 45.202 52.295 1.00 32.02 H ATOM 2459 CG ASP 104 115.093 44.90151.298 1.00 55.62 H ATOM 2460 OD1 ASP 104 114.753 44.583 50.136 1.0038.63 H ATOM 2461 OD2 ASP 104 116.286 44.985 51.663 1.00 61.09 H ATOM2462 C ASP 104 115.255 47.067 53.415 1.00 29.02 H ATOM 2463 O ASP 104115.021 47.775 52.440 1.00 33.69 H ATOM 2464 N TRP 105 116.148 47.38654.346 1.00 37.55 H ATOM 2465 CA TRP 105 116.907 48.628 54.288 1.0032.28 H ATOM 2466 CB TRP 105 116.599 49.484 55.518 1.00 42.44 H ATOM2467 CG TRP 105 115.123 49.751 55.717 1.00 44.69 H ATOM 2468 CD2 TRP 105114.456 50.028 56.955 1.00 43.59 H ATOM 2469 CE2 TRP 105 113.091 50.21756.662 1.00 39.38 H ATOM 2470 CE3 TRP 105 114.887 50.136 58.285 1.0053.16 H ATOM 2471 CD1 TRP 105 114.157 49.780 54.751 1.00 25.93 H ATOM2472 NE1 TRP 105 112.942 50.059 55.313 1.00 37.01 H ATOM 2473 CZ2 TRP105 112.144 50.507 57.650 1.00 45.54 H ATOM 2474 CZ3 TRP 105 113.94550.425 59.270 1.00 48.69 H ATOM 2475 CH2 TRP 105 112.588 50.609 58.9451.00 55.24 H ATOM 2476 C TRP 105 118.405 48.421 54.187 1.00 40.80 H ATOM2477 O TRP 105 119.063 49.042 53.352 1.00 47.85 H ATOM 2478 N ASN 106118.952 47.556 55.038 1.00 49.48 H ATOM 2479 CA ASN 106 120.389 47.30555.013 1.00 46.39 H ATOM 2480 CB ASN 106 121.103 48.280 55.951 1.0036.85 H ATOM 2481 CG ASN 106 120.869 47.961 57.408 1.00 28.91 H ATOM2482 OD1 ASN 106 119.909 48.434 58.013 1.00 32.97 H ATOM 2483 ND2 ASN106 121.744 47.152 57.981 1.00 41.71 H ATOM 2484 C ASN 106 120.78645.877 55.363 1.00 48.25 H ATOM 2485 O ASN 106 119.950 45.059 55.7601.00 46.92 H ATOM 2486 N PHE 107 122.079 45.602 55.202 1.00 47.37 H ATOM2487 CA PHE 107 122.665 44.294 55.489 1.00 47.06 H ATOM 2488 CB PHE 107123.589 43.862 54.341 1.00 51.22 H ATOM 2489 CG PHE 107 123.004 44.05252.954 1.00 36.58 H ATOM 2490 CD1 PHE 107 121.628 44.053 52.737 1.0043.99 H ATOM 2491 CD2 PHE 107 123.847 44.180 51.851 1.00 35.28 H ATOM2492 CE1 PHE 107 121.107 44.175 51.449 1.00 41.51 H ATOM 2493 CE2 PHE107 123.334 44.301 50.561 1.00 20.31 H ATOM 2494 CZ PHE 107 121.96544.296 50.362 1.00 40.17 H ATOM 2495 C PHE 107 123.479 44.386 56.7871.00 45.87 H ATOM 2496 O PHE 107 124.641 44.805 56.765 1.00 38.80 H ATOM2497 N ASP 108 122.874 43.982 57.905 1.00 44.31 H ATOM 2498 CA ASP 108123.528 44.053 59.215 1.00 40.48 H ATOM 2499 CB ASP 108 122.479 44.24360.325 1.00 54.96 H ATOM 2500 CG ASP 108 121.048 44.057 59.833 1.0066.26 H ATOM 2501 OD1 ASP 108 120.304 45.059 59.790 1.00 56.03 H ATOM2502 OD2 ASP 108 120.664 42.914 59.494 1.00 63.18 H ATOM 2503 C ASP 108124.445 42.889 59.600 1.00 27.53 H ATOM 2504 O ASP 108 125.418 43.07760.328 1.00 16.20 H ATOM 2505 N VAL 109 124.133 41.690 59.128 1.00 18.58H ATOM 2506 CA VAL 109 124.946 40.527 59.458 1.00 20.11 H ATOM 2507 CBVAL 109 124.072 39.410 60.037 1.00 25.57 H ATOM 2508 CG1 VAL 109 124.93638.353 60.691 1.00 22.40 H ATOM 2509 CG2 VAL 109 123.098 39.998 61.0381.00 21.93 H ATOM 2510 C VAL 109 125.704 39.984 58.246 1.00 26.64 H ATOM2511 O VAL 109 125.110 39.594 57.248 1.00 34.79 H ATOM 2512 N TRP 110127.023 39.949 58.336 1.00 27.30 H ATOM 2513 CA TRP 110 127.818 39.45957.229 1.00 27.28 H ATOM 2514 CB TRP 110 128.834 40.519 56.810 1.0033.78 H ATOM 2515 CG TRP 110 128.248 41.738 56.167 1.00 36.21 H ATOM2516 CD2 TRP 110 128.440 42.162 54.813 1.00 25.48 H ATOM 2517 CE2 TRP110 127.801 43.411 54.669 1.00 33.24 H ATOM 2518 CE3 TRP 110 129.09741.609 53.711 1.00 13.14 H ATOM 2519 CD1 TRP 110 127.511 42.715 56.7701.00 42.55 H ATOM 2520 NE1 TRP 110 127.236 43.728 55.879 1.00 36.86 HATOM 2521 CZ2 TRP 110 127.795 44.115 53.467 1.00 25.21 H ATOM 2522 CZ3TRP 110 129.096 42.314 52.511 1.00 30.00 H ATOM 2523 CH2 TRP 110 128.44943.553 52.402 1.00 26.50 H ATOM 2524 C TRP 110 128.566 38.187 57.6011.00 29.69 H ATOM 2525 O TRP 110 128.946 37.993 58.753 1.00 39.20 H ATOM2526 N GLY 111 128.774 37.317 56.621 1.00 26.69 H ATOM 2527 CA GLY 111129.523 36.110 56.880 1.00 15.66 H ATOM 2528 C GLY 111 130.988 36.50556.847 1.00 32.31 H ATOM 2529 O GLY 111 131.327 37.622 56.451 1.00 24.60H ATOM 2530 N ALA 112 131.867 35.604 57.261 1.00 41.72 H ATOM 2531 CAALA 112 133.287 35.907 57.249 1.00 41.34 H ATOM 2532 CB ALA 112 134.03334.911 58.119 1.00 39.24 H ATOM 2533 C ALA 112 133.857 35.896 55.8261.00 45.93 H ATOM 2534 O ALA 112 134.902 36.492 55.568 1.00 49.85 H ATOM2535 N GLY 113 133.178 35.217 54.906 1.00 45.09 H ATOM 2536 CA GLY 113133.665 35.161 53.538 1.00 44.98 H ATOM 2537 C GLY 113 134.180 33.79853.099 1.00 44.48 H ATOM 2538 O GLY 113 134.273 32.862 53.894 1.00 40.99H ATOM 2539 N THR 114 134.519 33.690 51.819 1.00 44.31 H ATOM 2540 CATHR 114 135.019 32.446 51.242 1.00 31.88 H ATOM 2541 CB THR 114 133.84531.530 50.772 1.00 20.38 H ATOM 2542 OG1 THR 114 133.279 30.861 51.9001.00 35.87 H ATOM 2543 CG2 THR 114 134.328 30.478 49.789 1.00 32.39 HATOM 2544 C THR 114 135.865 32.814 50.032 1.00 33.60 H ATOM 2545 O THR114 135.325 33.054 48.953 1.00 42.98 H ATOM 2546 N THR 115 137.18232.880 50.208 1.00 35.92 H ATOM 2547 CA THR 115 138.055 33.208 49.0891.00 33.21 H ATOM 2548 CB THR 115 139.525 33.317 49.499 1.00 43.78 HATOM 2549 OG1 THR 115 139.649 34.185 50.632 1.00 41.45 H ATOM 2550 CG2THR 115 140.351 33.867 48.332 1.00 43.50 H ATOM 2551 C THR 115 137.93832.100 48.059 1.00 39.18 H ATOM 2552 O THR 115 137.823 30.918 48.4061.00 28.11 H ATOM 2553 N VAL 116 137.958 32.490 46.791 1.00 36.51 H ATOM2554 CA VAL 116 137.840 31.537 45.708 1.00 40.64 H ATOM 2555 CB VAL 116136.443 31.616 45.051 1.00 41.04 H ATOM 2556 CG1 VAL 116 136.337 30.59943.931 1.00 35.25 H ATOM 2557 CG2 VAL 116 135.362 31.359 46.096 1.0033.28 H ATOM 2558 C VAL 116 138.899 31.816 44.661 1.00 43.76 H ATOM 2559O VAL 116 139.009 32.929 44.146 1.00 46.23 H ATOM 2560 N THR 117 139.68330.791 44.354 1.00 44.39 H ATOM 2561 CA THR 117 140.740 30.919 43.3701.00 40.89 H ATOM 2562 CB THR 117 142.095 30.552 43.969 1.00 38.40 HATOM 2563 OG1 THR 117 142.320 31.340 45.145 1.00 36.59 H ATOM 2564 CG2THR 117 143.205 30.810 42.966 1.00 32.70 H ATOM 2565 C THR 117 140.44930.001 42.203 1.00 38.02 H ATOM 2566 O THR 117 139.802 28.971 42.3571.00 37.58 H ATOM 2567 N VAL 118 140.928 30.386 41.031 1.00 45.45 H ATOM2568 CA VAL 118 140.705 29.597 39.838 1.00 47.20 H ATOM 2569 CB VAL 118140.199 30.481 38.694 1.00 54.07 H ATOM 2570 CG1 VAL 118 138.698 30.70438.844 1.00 40.64 H ATOM 2571 CG2 VAL 118 140.942 31.807 38.700 1.0046.30 H ATOM 2572 C VAL 118 141.973 28.890 39.400 1.00 42.28 H ATOM 2573O VAL 118 141.946 27.706 39.060 1.00 42.04 H ATOM 2574 N SER 119 143.08129.622 39.415 1.00 40.12 H ATOM 2575 CA SER 119 144.381 29.089 39.0151.00 48.99 H ATOM 2576 CB SER 119 145.469 29.569 39.983 1.00 49.51 HATOM 2577 OG SER 119 146.419 30.398 39.331 1.00 31.69 H ATOM 2578 C SER119 144.418 27.563 38.929 1.00 49.87 H ATOM 2579 O SER 119 144.02826.856 39.870 1.00 42.52 H ATOM 2580 N SER 120 144.887 27.067 37.7871.00 55.96 H ATOM 2581 CA SER 120 144.996 25.633 37.540 1.00 53.80 HATOM 2582 CB SER 120 144.774 25.334 36.050 1.00 57.59 H ATOM 2583 OG SER120 145.785 25.919 35.239 1.00 55.34 H ATOM 2584 C SER 120 146.37725.145 37.965 1.00 53.97 H ATOM 2585 O SER 120 146.879 24.149 37.4471.00 49.63 H ATOM 2586 N ALA 121 146.974 25.858 38.919 1.00 62.49 H ATOM2587 CA ALA 121 148.307 25.544 39.445 1.00 58.34 H ATOM 2588 CB ALA 121148.830 26.714 40.278 1.00 52.59 H ATOM 2589 C ALA 121 148.333 24.26840.284 1.00 54.04 H ATOM 2590 O ALA 121 147.318 23.584 40.432 1.00 56.38H ATOM 2591 N LYS 122 149.498 23.951 40.841 1.00 48.35 H ATOM 2592 CALYS 122 149.624 22.750 41.649 1.00 46.60 H ATOM 2593 CB LYS 122 150.91221.998 41.318 1.00 51.72 H ATOM 2594 CG LYS 122 150.682 20.565 40.8431.00 51.16 H ATOM 2595 CD LYS 122 150.117 19.675 41.948 1.00 40.43 HATOM 2596 CE LYS 122 151.191 18.754 42.525 1.00 52.69 H ATOM 2597 NZ LYS122 150.980 17.320 42.166 1.00 52.23 H ATOM 2598 C LYS 122 149.57723.043 43.133 1.00 52.24 H ATOM 2599 O LYS 122 150.258 23.933 43.6441.00 51.54 H ATOM 2600 N THR 123 148.758 22.255 43.811 1.00 58.75 H ATOM2601 CA THR 123 148.562 22.363 45.249 1.00 59.16 H ATOM 2602 CB THR 123147.422 21.459 45.722 1.00 70.62 H ATOM 2603 OG1 THR 123 146.234 21.77444.989 1.00 100.00 H ATOM 2604 CG2 THR 123 147.174 21.644 47.216 1.0071.56 H ATOM 2605 C THR 123 149.851 21.904 45.913 1.00 59.76 H ATOM 2606O THR 123 150.327 20.797 45.654 1.00 51.50 H ATOM 2607 N THR 124 150.43022.671 46.957 1.00 60.58 H ATOM 2608 CA THR 124 151.805 22.409 47.3391.00 54.63 H ATOM 2609 CB THR 124 152.776 23.187 46.433 1.00 44.65 HATOM 2610 OG1 THR 124 152.458 22.930 45.055 1.00 43.48 H ATOM 2611 CG2THR 124 154.207 22.779 46.719 1.00 35.75 H ATOM 2612 C THR 124 152.03422.844 48.776 1.00 61.90 H ATOM 2613 O THR 124 151.832 24.005 49.1131.00 66.47 H ATOM 2614 N PRO 125 152.470 21.918 49.644 1.00 63.12 H ATOM2615 CD PRO 125 152.792 20.505 49.387 1.00 43.60 H ATOM 2616 CA PRO 125152.705 22.286 51.044 1.00 64.11 H ATOM 2617 CB PRO 125 153.012 20.95651.725 1.00 53.31 H ATOM 2618 CG PRO 125 153.525 20.089 50.636 1.0027.09 H ATOM 2619 C PRO 125 153.858 23.274 51.141 1.00 60.12 H ATOM 2620O PRO 125 154.781 23.255 50.329 1.00 49.68 H ATOM 2621 N PRO 126 153.81124.153 52.140 1.00 55.19 H ATOM 2622 CD PRO 126 152.761 24.258 53.1691.00 61.07 H ATOM 2623 CA PRO 126 154.855 25.156 52.333 1.00 47.53 HATOM 2624 CB PRO 126 154.213 26.141 53.296 1.00 49.84 H ATOM 2625 CG PRO126 153.315 25.289 54.118 1.00 73.92 H ATOM 2626 C PRO 126 156.15524.608 52.891 1.00 49.11 H ATOM 2627 O PRO 126 156.148 23.695 53.7161.00 53.71 H ATOM 2628 N SER 127 157.266 25.174 52.429 1.00 38.85 H ATOM2629 CA SER 127 158.586 24.792 52.913 1.00 21.97 H ATOM 2630 CB SER 127159.628 24.904 51.798 1.00 39.50 H ATOM 2631 OG SER 127 159.487 23.86650.845 1.00 52.42 H ATOM 2632 C SER 127 158.864 25.826 53.990 1.00 33.98H ATOM 2633 O SER 127 159.021 27.008 53.687 1.00 34.88 H ATOM 2634 N VAL128 158.903 25.393 55.243 1.00 39.20 H ATOM 2635 CA VAL 128 159.13526.308 56.353 1.00 46.26 H ATOM 2636 CB VAL 128 158.247 25.929 57.5621.00 49.37 H ATOM 2637 CG1 VAL 128 158.162 27.089 58.545 1.00 42.90 HATOM 2638 CG2 VAL 128 156.862 25.538 57.078 1.00 51.53 H ATOM 2639 C VAL128 160.594 26.350 56.794 1.00 37.55 H ATOM 2640 O VAL 128 161.18325.329 57.153 1.00 49.56 H ATOM 2641 N TYR 129 161.172 27.545 56.7651.00 48.51 H ATOM 2642 CA TYR 129 162.562 27.740 57.150 1.00 44.86 HATOM 2643 CB TYR 129 163.364 28.279 55.969 1.00 35.54 H ATOM 2644 CG TYR129 163.348 27.402 54.736 1.00 27.55 H ATOM 2645 CD1 TYR 129 163.71826.059 54.800 1.00 42.44 H ATOM 2646 CE1 TYR 129 163.729 25.256 53.6541.00 44.45 H ATOM 2647 CD2 TYR 129 162.986 27.928 53.497 1.00 28.77 HATOM 2648 CE2 TYR 129 162.991 27.135 52.345 1.00 44.26 H ATOM 2649 CZTYR 129 163.363 25.806 52.430 1.00 46.95 H ATOM 2650 OH TYR 129 163.36625.036 51.291 1.00 71.81 H ATOM 2651 C TYR 129 162.670 28.722 58.3121.00 41.49 H ATOM 2652 O TYR 129 162.195 29.856 58.225 1.00 48.76 H ATOM2653 N PRO 130 163.309 28.302 59.415 1.00 46.24 H ATOM 2654 CD PRO 130163.913 26.972 59.604 1.00 58.08 H ATOM 2655 CA PRO 130 163.482 29.15260.597 1.00 40.62 H ATOM 2656 CB PRO 130 163.970 28.180 61.661 1.0049.21 H ATOM 2657 CG PRO 130 164.699 27.132 60.872 1.00 50.86 H ATOM2658 C PRO 130 164.486 30.276 60.359 1.00 42.13 H ATOM 2659 O PRO 130165.506 30.079 59.704 1.00 49.80 H ATOM 2660 N LEU 131 164.195 31.45560.895 1.00 40.92 H ATOM 2661 CA LEU 131 165.081 32.598 60.732 1.0035.23 H ATOM 2662 CB LEU 131 164.319 33.772 60.120 1.00 34.49 H ATOM2663 CG LEU 131 163.916 33.644 58.652 1.00 34.92 H ATOM 2664 CD1 LEU 131163.135 34.878 58.245 1.00 11.51 H ATOM 2665 CD2 LEU 131 165.144 33.48357.781 1.00 26.67 H ATOM 2666 C LEU 131 165.666 33.014 62.077 1.00 40.36H ATOM 2667 O LEU 131 164.956 33.557 62.927 1.00 46.95 H ATOM 2668 N ALA132 166.956 32.755 62.263 1.00 56.70 H ATOM 2669 CA ALA 132 167.63233.110 63.507 1.00 56.71 H ATOM 2670 CB ALA 132 168.300 31.886 64.0961.00 57.53 H ATOM 2671 C ALA 132 168.662 34.210 63.268 1.00 55.87 H ATOM2672 O ALA 132 169.276 34.279 62.204 1.00 54.54 H ATOM 2673 N PRO 133168.864 35.091 64.262 1.00 57.72 H ATOM 2674 CD PRO 133 168.161 35.06565.557 1.00 47.07 H ATOM 2675 CA PRO 133 169.815 36.206 64.187 1.0060.50 H ATOM 2676 CB PRO 133 169.496 37.030 65.431 1.00 59.11 H ATOM2677 CG PRO 133 168.946 36.033 66.393 1.00 46.85 H ATOM 2678 C PRO 133171.285 35.780 64.154 1.00 64.47 H ATOM 2679 O PRO 133 171.743 35.03065.021 1.00 63.82 H ATOM 2680 N GLY 134 172.021 36.276 63.161 1.00 66.38H ATOM 2681 CA GLY 134 173.429 35.937 63.030 1.00 54.50 H ATOM 2682 CGLY 134 174.356 36.531 64.074 1.00 60.13 H ATOM 2683 O GLY 134 175.51536.851 63.795 1.00 70.75 H ATOM 2684 N SER 135 173.893 36.652 65.2761.00 74.93 H ATOM 2685 CA SER 135 174.743 37.216 66.319 1.00 86.16 HATOM 2686 CB SER 135 176.038 36.396 66.439 1.00 90.22 H ATOM 2687 OG SER135 176.965 36.773 65.426 1.00 100.00 H ATOM 2688 C SER 135 175.09638.660 65.950 1.00 88.57 H ATOM 2689 O SER 135 176.088 38.928 65.2551.00 77.68 H ATOM 2690 N ALA 136 174.255 39.552 66.431 1.00 94.60 H ATOM2691 CA ALA 136 174.413 40.992 66.206 1.00 96.15 H ATOM 2692 CB ALA 136174.299 41.304 64.714 1.00 91.75 H ATOM 2693 C ALA 136 173.334 41.75966.962 1.00 99.99 H ATOM 2694 O ALA 136 172.158 41.398 66.918 1.00 99.98H ATOM 2695 N ALA 137 173.740 42.818 67.655 1.00 99.99 H ATOM 2696 CAALA 137 172.802 43.637 68.417 1.00 100.00 H ATOM 2697 CB ALA 137 173.56544.631 69.285 1.00 89.73 H ATOM 2698 C ALA 137 171.860 44.377 67.4741.00 99.97 H ATOM 2699 O ALA 137 172.119 45.518 67.094 1.00 99.95 H ATOM2700 N GLN 138 170.768 43.718 67.103 1.00 99.99 H ATOM 2701 CA GLN 138169.788 44.308 66.198 1.00 99.99 H ATOM 2702 CB GLN 138 168.740 43.26465.804 1.00 92.32 H ATOM 2703 CG GLN 138 167.946 42.702 66.972 1.0080.27 H ATOM 2704 CD GLN 138 166.461 42.627 66.679 1.00 62.10 H ATOM2705 OE1 GLN 138 166.051 42.463 65.530 1.00 72.23 H ATOM 2706 NE2 GLN138 165.646 42.749 67.720 1.00 47.02 H ATOM 2707 C GLN 138 169.10045.522 66.818 1.00 99.97 H ATOM 2708 O GLN 138 168.850 46.516 66.1361.00 99.97 H ATOM 2709 N THR 139 168.797 45.438 68.116 1.00 99.99 H ATOM2710 CA THR 139 168.138 46.530 68.810 1.00 100.00 H ATOM 2711 CB THR 139166.629 46.520 68.570 1.00 100.00 H ATOM 2712 OG1 THR 139 166.068 45.30669.085 1.00 99.99 H ATOM 2713 CG2 THR 139 166.326 46.637 67.084 1.0099.99 H ATOM 2714 C THR 139 168.421 46.461 70.301 1.00 99.99 H ATOM 2715O THR 139 167.865 45.622 71.014 1.00 99.98 H ATOM 2716 N ASN 140 169.28247.359 70.792 1.00 99.98 H ATOM 2717 CA ASN 140 169.654 47.435 72.1871.00 96.92 H ATOM 2718 CB ASN 140 168.714 48.381 72.927 1.00 94.82 HATOM 2719 CG ASN 140 168.783 49.786 72.357 1.00 91.71 H ATOM 2720 OD1ASN 140 169.866 50.280 72.032 1.00 88.37 H ATOM 2721 ND2 ASN 140 167.63350.433 72.229 1.00 91.05 H ATOM 2722 C ASN 140 169.724 46.052 72.8011.00 93.43 H ATOM 2723 O ASN 140 170.715 45.321 72.647 1.00 83.04 H ATOM2724 N SER 141 168.684 45.691 73.529 1.00 91.74 H ATOM 2725 CA SER 141168.629 44.391 74.200 1.00 78.99 H ATOM 2726 CB SER 141 168.101 44.54475.629 1.00 69.11 H ATOM 2727 OG SER 141 166.899 45.297 75.639 1.0060.50 H ATOM 2728 C SER 141 167.726 43.431 73.432 1.00 75.37 H ATOM 2729O SER 141 168.173 42.413 72.896 1.00 88.08 H ATOM 2730 N MET 142 166.47943.787 73.391 1.00 59.56 H ATOM 2731 CA MET 142 165.449 42.970 72.7661.00 44.97 H ATOM 2732 CB MET 142 164.206 43.807 72.516 1.00 56.70 HATOM 2733 CG MET 142 163.645 44.397 73.800 1.00 86.09 H ATOM 2734 SD MET142 162.177 45.354 73.535 1.00 99.97 H ATOM 2735 CE MET 142 162.23946.814 74.546 1.00 99.42 H ATOM 2736 C MET 142 165.871 42.372 71.4321.00 32.94 H ATOM 2737 O MET 142 166.780 42.880 70.772 1.00 33.63 H ATOM2738 N VAL 143 165.183 41.304 71.027 1.00 27.18 H ATOM 2739 CA VAL 143165.501 40.615 69.779 1.00 24.76 H ATOM 2740 CB VAL 143 166.197 39.27270.065 1.00 10.57 H ATOM 2741 CG1 VAL 143 165.186 38.268 70.594 1.0034.15 H ATOM 2742 CG2 VAL 143 166.872 38.750 68.800 1.00 21.19 H ATOM2743 C VAL 143 164.299 40.345 68.871 1.00 32.37 H ATOM 2744 O VAL 143163.150 40.451 69.293 1.00 31.65 H ATOM 2745 N THR 144 164.588 39.98167.623 1.00 32.76 H ATOM 2746 CA THR 144 163.561 39.686 66.630 1.0032.18 H ATOM 2747 CB THR 144 163.459 40.810 65.580 1.00 23.79 H ATOM2748 OG1 THR 144 162.934 41.991 66.196 1.00 53.98 H ATOM 2749 CG2 THR144 162.543 40.394 64.436 1.00 20.69 H ATOM 2750 C THR 144 163.85538.384 65.891 1.00 39.99 H ATOM 2751 O THR 144 164.958 38.178 65.3841.00 46.16 H ATOM 2752 N LEU 145 162.858 37.507 65.830 1.00 32.72 H ATOM2753 CA LEU 145 163.004 36.237 65.138 1.00 13.00 H ATOM 2754 CB LEU 145162.645 35.082 66.062 1.00 22.16 H ATOM 2755 CG LEU 145 163.748 34.61567.003 1.00 24.64 H ATOM 2756 CD1 LEU 145 163.529 35.237 68.376 1.0021.35 H ATOM 2757 CD2 LEU 145 163.736 33.095 67.084 1.00 18.63 H ATOM2758 C LEU 145 162.064 36.220 63.948 1.00 24.06 H ATOM 2759 O LEU 145161.188 37.073 63.831 1.00 32.59 H ATOM 2760 N GLY 146 162.244 35.24063.072 1.00 32.97 H ATOM 2761 CA GLY 146 161.397 35.153 61.903 1.0032.08 H ATOM 2762 C GLY 146 161.254 33.741 61.381 1.00 29.89 H ATOM 2763O GLY 146 161.995 32.845 61.778 1.00 19.61 H ATOM 2764 N CYS 147 160.28333.553 60.499 1.00 39.08 H ATOM 2765 CA CYS 147 160.023 32.258 59.8851.00 38.87 H ATOM 2766 C CYS 147 159.696 32.518 58.425 1.00 30.42 H ATOM2767 O CYS 147 158.934 33.431 58.109 1.00 37.75 H ATOM 2768 CB CYS 147158.843 31.560 60.574 1.00 55.35 H ATOM 2769 SG CYS 147 159.313 30.02161.435 1.00 81.70 H ATOM 2770 N LEU 148 160.288 31.730 57.536 1.00 28.82H ATOM 2771 CA LEU 148 160.037 31.891 56.115 1.00 27.56 H ATOM 2772 CBLEU 148 161.363 31.967 55.352 1.00 34.75 H ATOM 2773 CG LEU 148 161.25432.129 53.831 1.00 34.95 H ATOM 2774 CD1 LEU 148 160.549 33.425 53.4881.00 18.93 H ATOM 2775 CD2 LEU 148 162.636 32.111 53.219 1.00 21.69 HATOM 2776 C LEU 148 159.187 30.737 55.580 1.00 24.86 H ATOM 2777 O LEU148 159.576 29.572 55.663 1.00 30.70 H ATOM 2778 N VAL 149 158.02031.077 55.045 1.00 27.57 H ATOM 2779 CA VAL 149 157.091 30.096 54.4871.00 25.23 H ATOM 2780 CB VAL 149 155.658 30.376 54.969 1.00 3.47 H ATOM2781 CG1 VAL 149 154.800 29.127 54.827 1.00 25.66 H ATOM 2782 CG2 VAL149 155.695 30.830 56.417 1.00 15.58 H ATOM 2783 C VAL 149 157.15230.207 52.969 1.00 43.19 H ATOM 2784 O VAL 149 156.520 31.080 52.3751.00 55.54 H ATOM 2785 N LYS 150 157.906 29.313 52.341 1.00 45.51 H ATOM2786 CA LYS 150 158.078 29.377 50.897 1.00 36.67 H ATOM 2787 CB LYS 150159.572 29.376 50.570 1.00 23.27 H ATOM 2788 CG LYS 150 159.950 30.22649.366 1.00 37.80 H ATOM 2789 CD LYS 150 161.441 30.145 49.070 1.0045.44 H ATOM 2790 CE LYS 150 161.689 29.729 47.631 1.00 47.90 H ATOM2791 NZ LYS 150 163.035 30.147 47.149 1.00 74.67 H ATOM 2792 C LYS 150157.393 28.322 50.047 1.00 47.62 H ATOM 2793 O LYS 150 157.086 27.22550.503 1.00 61.91 H ATOM 2794 N GLY 151 157.172 28.686 48.789 1.00 52.19H ATOM 2795 CA GLY 151 156.562 27.808 47.805 1.00 56.42 H ATOM 2796 CGLY 151 155.344 26.981 48.176 1.00 65.95 H ATOM 2797 O GLY 151 155.40425.751 48.146 1.00 83.19 H ATOM 2798 N TYR 152 154.238 27.641 48.5221.00 68.59 H ATOM 2799 CA TYR 152 153.018 26.919 48.866 1.00 57.51 HATOM 2800 CB TYR 152 152.646 27.131 50.342 1.00 55.56 H ATOM 2801 CG TYR152 152.211 28.529 50.695 1.00 49.75 H ATOM 2802 CD1 TYR 152 150.89328.938 50.497 1.00 37.14 H ATOM 2803 CE1 TYR 152 150.474 30.222 50.8471.00 37.53 H ATOM 2804 CD2 TYR 152 153.104 29.437 51.250 1.00 44.59 HATOM 2805 CE2 TYR 152 152.696 30.726 51.605 1.00 49.92 H ATOM 2806 CZTYR 152 151.379 31.107 51.400 1.00 51.57 H ATOM 2807 OH TYR 152 150.96732.371 51.745 1.00 62.33 H ATOM 2808 C TYR 152 151.874 27.358 47.9651.00 48.01 H ATOM 2809 O TYR 152 151.962 28.386 47.291 1.00 28.34 H ATOM2810 N PHE 153 150.810 26.567 47.947 1.00 50.83 H ATOM 2811 CA PHE 153149.646 26.857 47.125 1.00 59.21 H ATOM 2812 CB PHE 153 150.003 26.72845.646 1.00 64.09 H ATOM 2813 CG PHE 153 148.926 27.214 44.725 1.0066.51 H ATOM 2814 CD1 PHE 153 148.786 28.571 44.464 1.00 66.83 H ATOM2815 CD2 PHE 153 148.036 26.323 44.147 1.00 60.06 H ATOM 2816 CE1 PHE153 147.770 29.037 43.638 1.00 55.52 H ATOM 2817 CE2 PHE 153 147.01326.775 43.316 1.00 50.07 H ATOM 2818 CZ PHE 153 146.880 28.138 43.0611.00 49.86 H ATOM 2819 C PHE 153 148.499 25.907 47.449 1.00 52.00 H ATOM2820 O PHE 153 148.709 24.702 47.619 1.00 57.40 H ATOM 2821 N PRO 154147.265 26.436 47.538 1.00 46.46 H ATOM 2822 CD PRO 154 146.057 25.64447.815 1.00 39.60 H ATOM 2823 CA PRO 154 146.931 27.851 47.353 1.0044.22 H ATOM 2824 CB PRO 154 145.533 27.795 46.772 1.00 48.46 H ATOM2825 CG PRO 154 144.923 26.614 47.482 1.00 49.48 H ATOM 2826 C PRO 154146.932 28.559 48.694 1.00 48.75 H ATOM 2827 O PRO 154 147.255 27.95849.722 1.00 45.97 H ATOM 2828 N GLU 155 146.573 29.835 48.683 1.00 45.51H ATOM 2829 CA GLU 155 146.508 30.585 49.927 1.00 46.54 H ATOM 2830 CBGLU 155 146.287 32.072 49.649 1.00 42.15 H ATOM 2831 CG GLU 155 147.56532.888 49.519 1.00 52.55 H ATOM 2832 CD GLU 155 147.866 33.696 50.7731.00 54.96 H ATOM 2833 OE1 GLU 155 148.005 33.096 51.862 1.00 38.84 HATOM 2834 OE2 GLU 155 147.964 34.938 50.667 1.00 57.00 H ATOM 2835 C GLU155 145.292 30.003 50.625 1.00 43.57 H ATOM 2836 O GLU 155 144.44529.388 49.974 1.00 52.29 H ATOM 2837 N PRO 156 145.203 30.148 51.9531.00 28.63 H ATOM 2838 CD PRO 156 143.990 29.751 52.682 1.00 21.85 HATOM 2839 CA PRO 156 146.134 30.804 52.873 1.00 32.78 H ATOM 2840 CB PRO156 145.212 31.685 53.699 1.00 41.77 H ATOM 2841 CG PRO 156 143.89630.824 53.775 1.00 10.50 H ATOM 2842 C PRO 156 146.879 29.797 53.7761.00 40.11 H ATOM 2843 O PRO 156 146.757 28.583 53.613 1.00 30.70 H ATOM2844 N VAL 157 147.641 30.333 54.722 1.00 47.16 H ATOM 2845 CA VAL 157148.383 29.540 55.693 1.00 58.27 H ATOM 2846 CB VAL 157 149.898 29.45255.362 1.00 52.20 H ATOM 2847 CG1 VAL 157 150.106 28.797 54.011 1.0056.74 H ATOM 2848 CG2 VAL 157 150.525 30.837 55.387 1.00 45.36 H ATOM2849 C VAL 157 148.239 30.258 57.032 1.00 68.19 H ATOM 2850 O VAL 157147.993 31.466 57.076 1.00 78.90 H ATOM 2851 N THR 158 148.395 29.52058.122 1.00 68.08 H ATOM 2852 CA THR 158 148.282 30.116 59.442 1.0063.24 H ATOM 2853 CB THR 158 147.209 29.396 60.279 1.00 61.67 H ATOM2854 OG1 THR 158 145.962 29.426 59.573 1.00 52.99 H ATOM 2855 CG2 THR158 147.034 30.079 61.632 1.00 56.86 H ATOM 2856 C THR 158 149.62330.035 60.154 1.00 64.54 H ATOM 2857 O THR 158 150.187 28.954 60.3121.00 65.02 H ATOM 2858 N VAL 159 150.131 31.190 60.571 1.00 48.72 H ATOM2859 CA VAL 159 151.409 31.266 61.256 1.00 38.04 H ATOM 2860 CB VAL 159152.384 32.209 60.517 1.00 35.68 H ATOM 2861 CG1 VAL 159 153.745 32.20761.195 1.00 13.69 H ATOM 2862 CG2 VAL 159 152.520 31.765 59.072 1.0035.33 H ATOM 2863 C VAL 159 151.239 31.746 62.690 1.00 32.37 H ATOM 2864O VAL 159 150.940 32.917 62.951 1.00 46.22 H ATOM 2865 N THR 160 151.43030.812 63.616 1.00 34.81 H ATOM 2866 CA THR 160 151.316 31.088 65.0371.00 30.02 H ATOM 2867 CB THR 160 150.305 30.146 65.702 1.00 29.19 HATOM 2868 OG1 THR 160 149.788 29.230 64.729 1.00 41.86 H ATOM 2869 CG2THR 160 149.165 30.943 66.295 1.00 40.64 H ATOM 2870 C THR 160 152.67630.872 65.681 1.00 28.21 H ATOM 2871 O THR 160 153.501 30.118 65.1691.00 44.55 H ATOM 2872 N TRP 161 152.909 31.546 66.800 1.00 43.52 H ATOM2873 CA TRP 161 154.171 31.404 67.506 1.00 54.28 H ATOM 2874 CB TRP 161154.833 32.773 67.672 1.00 46.91 H ATOM 2875 CG TRP 161 155.369 33.32766.379 1.00 31.10 H ATOM 2876 CD2 TRP 161 156.648 33.059 65.790 1.0039.01 H ATOM 2877 CE2 TRP 161 156.710 33.782 64.579 1.00 43.01 H ATOM2878 CE3 TRP 161 157.748 32.277 66.169 1.00 31.39 H ATOM 2879 CD1 TRP161 154.727 34.179 65.525 1.00 30.94 H ATOM 2880 NE1 TRP 161 155.52534.458 64.443 1.00 31.32 H ATOM 2881 CZ2 TRP 161 157.829 33.749 63.7411.00 35.89 H ATOM 2882 CZ3 TRP 161 158.861 32.242 65.335 1.00 23.31 HATOM 2883 CH2 TRP 161 158.893 32.975 64.134 1.00 38.71 H ATOM 2884 C TRP161 153.920 30.746 68.862 1.00 57.21 H ATOM 2885 O TRP 161 153.15731.255 69.691 1.00 61.27 H ATOM 2886 N ASN 162 154.569 29.602 69.0711.00 56.37 H ATOM 2887 CA ASN 162 154.426 28.829 70.298 1.00 50.72 HATOM 2888 CB ASN 162 154.818 29.676 71.515 1.00 39.98 H ATOM 2889 CG ASN162 156.331 29.771 71.698 1.00 45.87 H ATOM 2890 OD1 ASN 162 157.10329.298 70.854 1.00 61.54 H ATOM 2891 ND2 ASN 162 156.760 30.385 72.8011.00 49.15 H ATOM 2892 C ASN 162 152.979 28.365 70.399 1.00 44.59 H ATOM2893 O ASN 162 152.387 28.329 71.479 1.00 33.04 H ATOM 2894 N SER 163152.419 28.021 69.242 1.00 55.71 H ATOM 2895 CA SER 163 151.048 27.54169.133 1.00 65.28 H ATOM 2896 CB SER 163 150.858 26.313 70.021 1.0069.26 H ATOM 2897 OG SER 163 151.917 25.391 69.823 1.00 87.88 H ATOM2898 C SER 163 149.993 28.592 69.468 1.00 64.07 H ATOM 2899 O SER 163148.799 28.363 69.267 1.00 71.86 H ATOM 2900 N GLY 164 150.430 29.74469.970 1.00 56.72 H ATOM 2901 CA GLY 164 149.490 30.798 70.308 1.0044.67 H ATOM 2902 C GLY 164 149.953 31.629 71.482 1.00 45.89 H ATOM 2903O GLY 164 149.611 32.807 71.597 1.00 41.13 H ATOM 2904 N SER 165 150.73931.011 72.355 1.00 56.45 H ATOM 2905 CA SER 165 151.259 31.688 73.5331.00 57.02 H ATOM 2906 CB SER 165 152.169 30.745 74.316 1.00 62.37 HATOM 2907 OG SER 165 151.418 29.663 74.836 1.00 50.35 H ATOM 2908 C SER165 152.020 32.945 73.153 1.00 57.97 H ATOM 2909 O SER 165 152.27633.809 73.989 1.00 41.07 H ATOM 2910 N LEU 166 152.385 33.049 71.8831.00 64.03 H ATOM 2911 CA LEU 166 153.102 34.227 71.421 1.00 56.74 HATOM 2912 CB LEU 166 154.493 33.841 70.923 1.00 37.44 H ATOM 2913 CG LEU166 155.626 34.088 71.922 1.00 41.79 H ATOM 2914 CD1 LEU 166 156.94833.782 71.254 1.00 36.73 H ATOM 2915 CD2 LEU 166 155.598 35.532 72.4161.00 58.45 H ATOM 2916 C LEU 166 152.318 34.920 70.319 1.00 60.22 H ATOM2917 O LEU 166 152.399 34.547 69.147 1.00 63.00 H ATOM 2918 N SER 167151.547 35.928 70.708 1.00 65.96 H ATOM 2919 CA SER 167 150.740 36.68469.762 1.00 64.77 H ATOM 2920 CB SER 167 149.255 36.347 69.946 1.0066.65 H ATOM 2921 OG SER 167 148.697 37.030 71.057 1.00 54.23 H ATOM2922 C SER 167 150.978 38.172 69.977 1.00 65.40 H ATOM 2923 O SER 167150.141 39.009 69.637 1.00 63.94 H ATOM 2924 N SER 168 152.134 38.48670.548 1.00 72.22 H ATOM 2925 CA SER 168 152.515 39.868 70.814 1.0066.97 H ATOM 2926 CB SER 168 152.814 40.052 72.306 1.00 83.10 H ATOM2927 OG SER 168 153.476 41.282 72.551 1.00 92.96 H ATOM 2928 C SER 168153.754 40.212 69.997 1.00 58.05 H ATOM 2929 O SER 168 154.694 39.42269.922 1.00 58.07 H ATOM 2930 N GLY 169 153.754 41.392 69.381 1.00 49.62H ATOM 2931 CA GLY 169 154.897 41.799 68.582 1.00 43.99 H ATOM 2932 CGLY 169 155.088 40.916 67.366 1.00 44.05 H ATOM 2933 O GLY 169 156.20540.731 66.887 1.00 49.35 H ATOM 2934 N VAL 170 153.991 40.366 66.8621.00 45.75 H ATOM 2935 CA VAL 170 154.058 39.506 65.697 1.00 34.35 HATOM 2936 CB VAL 170 153.135 38.291 65.854 1.00 29.09 H ATOM 2937 CG1VAL 170 153.213 37.418 64.614 1.00 23.76 H ATOM 2938 CG2 VAL 170 153.53937.492 67.079 1.00 30.40 H ATOM 2939 C VAL 170 153.660 40.269 64.4451.00 37.22 H ATOM 2940 O VAL 170 152.732 41.074 64.460 1.00 35.69 H ATOM2941 N HIS 171 154.383 40.017 63.364 1.00 38.69 H ATOM 2942 CA HIS 171154.118 40.664 62.088 1.00 33.69 H ATOM 2943 CB HIS 171 155.167 41.74361.802 1.00 38.36 H ATOM 2944 CG HIS 171 155.011 42.971 62.636 1.0040.32 H ATOM 2945 CD2 HIS 171 155.806 44.055 62.783 1.00 49.35 H ATOM2946 ND1 HIS 171 153.923 43.182 63.456 1.00 58.85 H ATOM 2947 CE1 HIS171 154.053 44.343 64.068 1.00 58.33 H ATOM 2948 NE2 HIS 171 155.18944.896 63.678 1.00 51.33 H ATOM 2949 C HIS 171 154.172 39.623 60.9851.00 37.90 H ATOM 2950 O HIS 171 155.251 39.144 60.626 1.00 52.25 H ATOM2951 N THR 172 153.012 39.250 60.465 1.00 36.40 H ATOM 2952 CA THR 172152.974 38.289 59.379 1.00 37.99 H ATOM 2953 CB THR 172 151.865 37.25159.576 1.00 30.37 H ATOM 2954 OG1 THR 172 152.160 36.452 60.729 1.0016.22 H ATOM 2955 CG2 THR 172 151.760 36.351 58.356 1.00 18.72 H ATOM2956 C THR 172 152.708 39.097 58.118 1.00 43.29 H ATOM 2957 O THR 172151.690 39.782 58.011 1.00 42.87 H ATOM 2958 N PHE 173 153.635 39.03157.172 1.00 42.03 H ATOM 2959 CA PHE 173 153.500 39.783 55.935 1.0041.59 H ATOM 2960 CB PHE 173 154.889 40.102 55.372 1.00 69.43 H ATOM2961 CG PHE 173 155.707 40.945 56.285 1.00 69.16 H ATOM 2962 CD1 PHE 173155.577 42.326 56.264 1.00 69.14 H ATOM 2963 CD2 PHE 173 156.555 40.36057.216 1.00 71.48 H ATOM 2964 CE1 PHE 173 156.277 43.117 57.165 1.0047.16 H ATOM 2965 CE2 PHE 173 157.260 41.140 58.122 1.00 46.77 H ATOM2966 CZ PHE 173 157.120 42.523 58.097 1.00 50.06 H ATOM 2967 C PHE 173152.651 39.103 54.870 1.00 51.94 H ATOM 2968 O PHE 173 152.670 37.88154.715 1.00 45.37 H ATOM 2969 N PRO 174 151.873 39.901 54.128 1.00 47.56H ATOM 2970 CD PRO 174 151.760 41.360 54.268 1.00 40.08 H ATOM 2971 CAPRO 174 151.010 39.369 53.070 1.00 39.95 H ATOM 2972 CB PRO 174 150.41740.618 52.419 1.00 38.02 H ATOM 2973 CG PRO 174 151.234 41.778 52.9451.00 41.70 H ATOM 2974 C PRO 174 151.793 38.515 52.080 1.00 34.30 H ATOM2975 O PRO 174 152.926 38.844 51.719 1.00 38.61 H ATOM 2976 N ALA 175151.191 37.407 51.655 1.00 33.98 H ATOM 2977 CA ALA 175 151.834 36.50550.711 1.00 28.24 H ATOM 2978 CB ALA 175 150.947 35.303 50.455 1.0024.04 H ATOM 2979 C ALA 175 152.106 37.226 49.411 1.00 43.10 H ATOM 2980O ALA 175 151.420 38.182 49.064 1.00 41.83 H ATOM 2981 N VAL 176 153.11936.758 48.691 1.00 47.34 H ATOM 2982 CA VAL 176 153.479 37.340 47.4091.00 46.04 H ATOM 2983 CB VAL 176 154.660 38.335 47.546 1.00 48.76 HATOM 2984 CG1 VAL 176 155.822 37.686 48.280 1.00 50.38 H ATOM 2985 CG2VAL 176 155.100 38.809 46.167 1.00 64.07 H ATOM 2986 C VAL 176 153.85836.205 46.462 1.00 36.12 H ATOM 2987 O VAL 176 154.544 35.263 46.8571.00 30.73 H ATOM 2988 N LEU 177 153.392 36.298 45.220 1.00 48.02 H ATOM2989 CA LEU 177 153.647 35.282 44.205 1.00 60.32 H ATOM 2990 CB LEU 177152.686 35.467 43.036 1.00 39.51 H ATOM 2991 CG LEU 177 152.048 34.16542.557 1.00 27.99 H ATOM 2992 CD1 LEU 177 151.014 33.698 43.572 1.0030.57 H ATOM 2993 CD2 LEU 177 151.410 34.378 41.197 1.00 38.71 H ATOM2994 C LEU 177 155.074 35.257 43.679 1.00 73.61 H ATOM 2995 O LEU 177155.621 36.275 43.254 1.00 78.42 H ATOM 2996 N GLN 178 155.669 34.07443.694 1.00 79.08 H ATOM 2997 CA GLN 178 157.031 33.895 43.227 1.0095.82 H ATOM 2998 CB GLN 178 157.981 33.932 44.432 1.00 99.99 H ATOM2999 CG GLN 178 159.244 33.098 44.308 1.00 99.99 H ATOM 3000 CD GLN 178160.357 33.583 45.224 1.00 99.99 H ATOM 3001 OE1 GLN 178 160.327 33.36746.439 1.00 98.52 H ATOM 3002 NE2 GLN 178 161.350 34.244 44.637 1.0099.99 H ATOM 3003 C GLN 178 157.123 32.564 42.483 1.00 99.99 H ATOM 3004O GLN 178 157.289 31.504 43.092 1.00 99.97 H ATOM 3005 N SER 179 156.98432.637 41.159 1.00 96.75 H ATOM 3006 CA SER 179 157.047 31.465 40.2891.00 87.63 H ATOM 3007 CB SER 179 158.307 30.641 40.589 1.00 93.06 HATOM 3008 OG SER 179 159.098 30.472 39.425 1.00 67.71 H ATOM 3009 C SER179 155.815 30.580 40.430 1.00 81.94 H ATOM 3010 O SER 179 155.92729.351 40.461 1.00 85.05 H ATOM 3011 N ASP 180 154.649 31.217 40.5171.00 61.79 H ATOM 3012 CA ASP 180 153.367 30.518 40.643 1.00 53.42 HATOM 3013 CB ASP 180 153.284 29.372 39.633 1.00 63.39 H ATOM 3014 CG ASP180 152.902 29.846 38.251 1.00 90.41 H ATOM 3015 OD1 ASP 180 151.73630.262 38.066 1.00 95.28 H ATOM 3016 OD2 ASP 180 153.771 29.804 37.3501.00 91.35 H ATOM 3017 C ASP 180 153.036 29.975 42.030 1.00 48.83 H ATOM3018 O ASP 180 151.965 29.414 42.234 1.00 32.55 H ATOM 3019 N LEU 181153.955 30.120 42.976 1.00 49.73 H ATOM 3020 CA LEU 181 153.716 29.64644.332 1.00 42.63 H ATOM 3021 CB LEU 181 154.751 28.594 44.730 1.0051.61 H ATOM 3022 CG LEU 181 154.817 27.335 43.865 1.00 45.19 H ATOM3023 CD1 LEU 181 155.788 26.358 44.503 1.00 36.88 H ATOM 3024 CD2 LEU181 153.429 26.712 43.723 1.00 49.90 H ATOM 3025 C LEU 181 153.81130.840 45.265 1.00 36.09 H ATOM 3026 O LEU 181 154.401 31.859 44.9141.00 47.48 H ATOM 3027 N TYR 182 153.235 30.711 46.454 1.00 34.48 H ATOM3028 CA TYR 182 153.254 31.803 47.410 1.00 34.99 H ATOM 3029 CB TYR 182151.965 31.824 48.221 1.00 43.15 H ATOM 3030 CG TYR 182 150.768 32.26647.431 1.00 26.23 H ATOM 3031 CD1 TYR 182 150.514 33.621 47.219 1.0024.46 H ATOM 3032 CE1 TYR 182 149.437 34.034 46.455 1.00 26.76 H ATOM3033 CD2 TYR 182 149.908 31.332 46.864 1.00 28.50 H ATOM 3034 CE2 TYR182 148.827 31.733 46.097 1.00 40.52 H ATOM 3035 CZ TYR 182 148.59633.085 45.893 1.00 31.63 H ATOM 3036 OH TYR 182 147.538 33.480 45.1081.00 52.38 H ATOM 3037 C TYR 182 154.410 31.694 48.361 1.00 34.67 H ATOM3038 O TYR 182 154.902 30.604 48.642 1.00 30.57 H ATOM 3039 N THR 183154.842 32.844 48.860 1.00 46.64 H ATOM 3040 CA THR 183 155.915 32.91449.838 1.00 32.69 H ATOM 3041 CB THR 183 157.250 33.348 49.232 1.0032.26 H ATOM 3042 OG1 THR 183 157.678 32.396 48.250 1.00 39.58 H ATOM3043 CG2 THR 183 158.288 33.442 50.318 1.00 33.99 H ATOM 3044 C THR 183155.483 33.981 50.823 1.00 28.00 H ATOM 3045 O THR 183 154.927 35.00950.440 1.00 38.20 H ATOM 3046 N LEU 184 155.737 33.743 52.097 1.00 27.54H ATOM 3047 CA LEU 184 155.353 34.696 53.114 1.00 30.19 H ATOM 3048 CBLEU 184 153.926 34.380 53.559 1.00 30.95 H ATOM 3049 CG LEU 184 153.48334.617 54.996 1.00 46.42 H ATOM 3050 CD1 LEU 184 151.969 34.814 55.0161.00 25.32 H ATOM 3051 CD2 LEU 184 153.891 33.439 55.867 1.00 45.00 HATOM 3052 C LEU 184 156.337 34.598 54.271 1.00 30.88 H ATOM 3053 O LEU184 157.075 33.623 54.383 1.00 17.03 H ATOM 3054 N SER 185 156.37035.613 55.122 1.00 29.51 H ATOM 3055 CA SER 185 157.278 35.599 56.2521.00 24.01 H ATOM 3056 CB SER 185 158.573 36.350 55.914 1.00 21.45 HATOM 3057 OG SER 185 158.354 37.749 55.844 1.00 46.01 H ATOM 3058 C SER185 156.610 36.235 57.449 1.00 28.20 H ATOM 3059 O SER 185 155.82837.173 57.312 1.00 26.88 H ATOM 3060 N SER 186 156.917 35.710 58.6251.00 38.58 H ATOM 3061 CA SER 186 156.354 36.229 59.853 1.00 34.31 HATOM 3062 CB SER 186 155.389 35.210 60.463 1.00 37.09 H ATOM 3063 OG SER186 154.862 35.685 61.689 1.00 44.43 H ATOM 3064 C SER 186 157.48736.510 60.822 1.00 25.01 H ATOM 3065 O SER 186 158.356 35.663 61.0361.00 38.29 H ATOM 3066 N SER 187 157.491 37.703 61.395 1.00 15.70 H ATOM3067 CA SER 187 158.524 38.066 62.348 1.00 25.23 H ATOM 3068 CB SER 187159.100 39.440 62.008 1.00 18.98 H ATOM 3069 OG SER 187 158.187 40.46662.346 1.00 36.58 H ATOM 3070 C SER 187 157.905 38.092 63.739 1.00 27.63H ATOM 3071 O SER 187 156.692 38.240 63.882 1.00 26.02 H ATOM 3072 N VAL188 158.739 37.926 64.762 1.00 31.12 H ATOM 3073 CA VAL 188 158.26637.950 66.141 1.00 31.94 H ATOM 3074 CB VAL 188 157.996 36.524 66.6811.00 32.97 H ATOM 3075 CG1 VAL 188 159.298 35.776 66.878 1.00 21.32 HATOM 3076 CG2 VAL 188 157.221 36.606 67.987 1.00 48.09 H ATOM 3077 C VAL188 159.284 38.646 67.030 1.00 29.00 H ATOM 3078 O VAL 188 160.46938.322 67.016 1.00 18.81 H ATOM 3079 N THR 189 158.811 39.618 67.7991.00 43.09 H ATOM 3080 CA THR 189 159.664 40.385 68.691 1.00 52.62 HATOM 3081 CB THR 189 159.381 41.890 68.535 1.00 55.64 H ATOM 3082 OG1THR 189 159.915 42.346 67.288 1.00 72.06 H ATOM 3083 CG2 THR 189 160.00642.678 69.676 1.00 47.82 H ATOM 3084 C THR 189 159.450 39.981 70.1471.00 47.05 H ATOM 3085 O THR 189 158.313 39.914 70.622 1.00 51.99 H ATOM3086 N VAL 190 160.547 39.715 70.846 1.00 33.42 H ATOM 3087 CA VAL 190160.489 39.327 72.251 1.00 49.20 H ATOM 3088 CB VAL 190 160.501 37.79572.408 1.00 46.79 H ATOM 3089 CG1 VAL 190 159.222 37.205 71.839 1.0024.58 H ATOM 3090 CG2 VAL 190 161.717 37.217 71.715 1.00 27.47 H ATOM3091 C VAL 190 161.670 39.893 73.037 1.00 57.65 H ATOM 3092 O VAL 190162.797 39.935 72.538 1.00 35.32 H ATOM 3093 N PRO 191 161.419 40.34774.277 1.00 74.09 H ATOM 3094 CD PRO 191 160.096 40.363 74.931 1.0085.05 H ATOM 3095 CA PRO 191 162.461 40.912 75.142 1.00 73.28 H ATOM3096 CB PRO 191 161.769 41.033 76.496 1.00 85.43 H ATOM 3097 CG PRO 191160.328 41.206 76.155 1.00 90.65 H ATOM 3098 C PRO 191 163.708 40.03175.207 1.00 69.24 H ATOM 3099 O PRO 191 163.618 38.814 75.392 1.00 45.94H ATOM 3100 N SER 192 164.871 40.663 75.063 1.00 75.06 H ATOM 3101 CASER 192 166.146 39.952 75.082 1.00 71.45 H ATOM 3102 CB SER 192 167.31140.955 75.103 1.00 59.57 H ATOM 3103 OG SER 192 167.154 41.923 76.1291.00 83.36 H ATOM 3104 C SER 192 166.291 38.977 76.248 1.00 74.48 H ATOM3105 O SER 192 167.095 38.051 76.184 1.00 78.55 H ATOM 3106 N SER 193165.498 39.169 77.297 1.00 78.60 H ATOM 3107 CA SER 193 165.574 38.31578.476 1.00 75.62 H ATOM 3108 CB SER 193 164.901 39.019 79.661 1.0088.02 H ATOM 3109 OG SER 193 163.490 39.027 79.525 1.00 99.66 H ATOM3110 C SER 193 165.013 36.898 78.345 1.00 67.52 H ATOM 3111 O SER 193165.430 35.998 79.073 1.00 55.75 H ATOM 3112 N THR 194 164.083 36.68477.418 1.00 72.49 H ATOM 3113 CA THR 194 163.464 35.365 77.259 1.0071.00 H ATOM 3114 CB THR 194 161.967 35.507 76.936 1.00 85.22 H ATOM3115 OG1 THR 194 161.795 36.425 75.846 1.00 87.88 H ATOM 3116 CG2 THR194 161.213 36.023 78.154 1.00 86.98 H ATOM 3117 C THR 194 164.08234.432 76.219 1.00 51.89 H ATOM 3118 O THR 194 163.726 33.253 76.1501.00 40.90 H ATOM 3119 N TRP 195 164.996 34.957 75.408 1.00 50.33 H ATOM3120 CA TRP 195 165.654 34.154 74.381 1.00 48.60 H ATOM 3121 CB TRP 195165.109 34.515 72.990 1.00 43.12 H ATOM 3122 CG TRP 195 165.338 33.44471.945 1.00 50.92 H ATOM 3123 CD2 TRP 195 166.332 33.451 70.907 1.0056.48 H ATOM 3124 CE2 TRP 195 166.199 32.235 70.192 1.00 47.66 H ATOM3125 CE3 TRP 195 167.320 34.365 70.513 1.00 53.35 H ATOM 3126 CD1 TRP195 164.664 32.259 71.817 1.00 57.45 H ATOM 3127 NE1 TRP 195 165.17531.528 70.766 1.00 58.35 H ATOM 3128 CZ2 TRP 195 167.019 31.911 69.1041.00 45.39 H ATOM 3129 CZ3 TRP 195 168.136 34.040 69.430 1.00 48.84 HATOM 3130 CH2 TRP 195 167.979 32.821 68.741 1.00 37.86 H ATOM 3131 C TRP195 167.164 34.383 74.420 1.00 55.91 H ATOM 3132 O TRP 195 167.62035.492 74.702 1.00 67.87 H ATOM 3133 N PRO 196 167.960 33.334 74.1431.00 55.52 H ATOM 3134 CD PRO 196 169.426 33.449 74.122 1.00 48.86 HATOM 3135 CA PRO 196 167.526 31.972 73.804 1.00 46.54 H ATOM 3136 CB PRO196 168.764 31.339 73.154 1.00 39.17 H ATOM 3137 CG PRO 196 169.81232.444 73.087 1.00 48.88 H ATOM 3138 C PRO 196 167.047 31.179 75.0161.00 48.13 H ATOM 3139 O PRO 196 166.920 29.951 74.958 1.00 51.32 H ATOM3140 N SER 197 166.789 31.891 76.109 1.00 63.32 H ATOM 3141 CA SER 197166.315 31.282 77.343 1.00 62.46 H ATOM 3142 CB SER 197 165.718 32.35378.255 1.00 69.33 H ATOM 3143 OG SER 197 166.324 32.329 79.533 1.0075.74 H ATOM 3144 C SER 197 165.261 30.225 77.029 1.00 55.85 H ATOM 3145O SER 197 165.557 29.029 76.979 1.00 63.63 H ATOM 3146 N GLU 198 164.03230.680 76.809 1.00 50.23 H ATOM 3147 CA GLU 198 162.925 29.787 76.4941.00 57.31 H ATOM 3148 CB GLU 198 161.605 30.416 76.945 1.00 72.95 HATOM 3149 CG GLU 198 161.645 30.972 78.360 1.00 75.15 H ATOM 3150 CD GLU198 160.646 32.093 78.584 1.00 85.11 H ATOM 3151 OE1 GLU 198 159.81732.341 77.680 1.00 99.04 H ATOM 3152 OE2 GLU 198 160.689 32.725 79.6641.00 77.93 H ATOM 3153 C GLU 198 162.889 29.516 74.995 1.00 56.54 H ATOM3154 O GLU 198 163.247 30.384 74.201 1.00 63.21 H ATOM 3155 N THR 199162.462 28.314 74.614 1.00 54.93 H ATOM 3156 CA THR 199 162.392 27.93773.203 1.00 53.51 H ATOM 3157 CB THR 199 162.304 26.400 73.031 1.0061.18 H ATOM 3158 OG1 THR 199 161.128 25.903 73.681 1.00 51.25 H ATOM3159 CG2 THR 199 163.536 25.727 73.623 1.00 78.07 H ATOM 3160 C THR 199161.205 28.583 72.485 1.00 59.63 H ATOM 3161 O THR 199 160.120 28.73073.054 1.00 59.98 H ATOM 3162 N VAL 200 161.429 28.966 71.228 1.00 57.25H ATOM 3163 CA VAL 200 160.407 29.603 70.405 1.00 51.96 H ATOM 3164 CBVAL 200 160.815 31.039 70.035 1.00 33.42 H ATOM 3165 CG1 VAL 200 159.95131.540 68.899 1.00 17.75 H ATOM 3166 CG2 VAL 200 160.680 31.944 71.2391.00 31.97 H ATOM 3167 C VAL 200 160.195 28.832 69.112 1.00 51.65 H ATOM3168 O VAL 200 161.136 28.613 68.353 1.00 62.16 H ATOM 3169 N THR 201158.955 28.427 68.862 1.00 39.44 H ATOM 3170 CA THR 201 158.631 27.68467.648 1.00 32.31 H ATOM 3171 CB THR 201 158.116 26.267 67.969 1.0041.49 H ATOM 3172 OG1 THR 201 159.042 25.602 68.831 1.00 51.63 H ATOM3173 CG2 THR 201 157.949 25.458 66.688 1.00 39.87 H ATOM 3174 C THR 201157.545 28.399 66.860 1.00 26.58 H ATOM 3175 O THR 201 156.682 29.05567.438 1.00 25.18 H ATOM 3176 N CYS 202 157.593 28.279 65.538 1.00 22.39H ATOM 3177 CA CYS 202 156.582 28.902 64.699 1.00 25.43 H ATOM 3178 CCYS 202 155.765 27.777 64.064 1.00 25.96 H ATOM 3179 O CYS 202 156.32426.826 63.522 1.00 34.22 H ATOM 3180 CB CYS 202 157.243 29.820 63.6491.00 49.34 H ATOM 3181 SG CYS 202 157.564 29.150 61.983 1.00 74.97 HATOM 3182 N ASN 203 154.441 27.871 64.172 1.00 24.52 H ATOM 3183 CA ASN203 153.554 26.842 63.628 1.00 29.46 H ATOM 3184 CB ASN 203 152.53926.387 64.686 1.00 36.69 H ATOM 3185 CG ASN 203 152.926 26.807 66.0991.00 44.66 H ATOM 3186 OD1 ASN 203 152.586 27.904 66.552 1.00 28.02 HATOM 3187 ND2 ASN 203 153.629 25.926 66.805 1.00 33.32 H ATOM 3188 C ASN203 152.800 27.293 62.389 1.00 18.74 H ATOM 3189 O ASN 203 151.96428.196 62.447 1.00 38.03 H ATOM 3190 N VAL 204 153.099 26.646 61.2701.00 23.45 H ATOM 3191 CA VAL 204 152.451 26.957 60.006 1.00 38.87 HATOM 3192 CB VAL 204 153.473 27.049 58.853 1.00 39.50 H ATOM 3193 CG1VAL 204 152.795 27.575 57.599 1.00 52.52 H ATOM 3194 CG2 VAL 204 154.62427.944 59.254 1.00 29.99 H ATOM 3195 C VAL 204 151.461 25.854 59.6761.00 41.89 H ATOM 3196 O VAL 204 151.733 24.682 59.919 1.00 46.77 H ATOM3197 N ALA 205 150.312 26.236 59.126 1.00 41.52 H ATOM 3198 CA ALA 205149.285 25.273 58.751 1.00 32.14 H ATOM 3199 CB ALA 205 148.156 25.28159.777 1.00 44.74 H ATOM 3200 C ALA 205 148.749 25.614 57.365 1.00 28.16H ATOM 3201 O ALA 205 148.349 26.750 57.104 1.00 23.06 H ATOM 3202 N HIS206 148.756 24.629 56.474 1.00 38.89 H ATOM 3203 CA HIS 206 148.26724.825 55.121 1.00 31.01 H ATOM 3204 CB HIS 206 149.357 24.497 54.1121.00 34.08 H ATOM 3205 CG HIS 206 149.025 24.909 52.715 1.00 25.51 HATOM 3206 CD2 HIS 206 148.521 26.065 52.227 1.00 33.37 H ATOM 3207 ND1HIS 206 149.193 24.073 51.635 1.00 28.36 H ATOM 3208 CE1 HIS 206 148.80724.698 50.535 1.00 30.36 H ATOM 3209 NE2 HIS 206 148.393 25.907 50.8681.00 29.53 H ATOM 3210 C HIS 206 147.061 23.930 54.876 1.00 38.48 H ATOM3211 O HIS 206 147.198 22.819 54.371 1.00 42.18 H ATOM 3212 N PRO 207145.856 24.417 55.228 1.00 46.11 H ATOM 3213 CD PRO 207 145.636 25.75855.802 1.00 59.24 H ATOM 3214 CA PRO 207 144.597 23.684 55.065 1.0049.09 H ATOM 3215 CB PRO 207 143.538 24.780 55.138 1.00 55.68 H ATOM3216 CG PRO 207 144.146 25.814 56.018 1.00 65.42 H ATOM 3217 C PRO 207144.504 22.890 53.769 1.00 60.16 H ATOM 3218 O PRO 207 144.293 21.68053.787 1.00 51.48 H ATOM 3219 N ALA 208 144.664 23.577 52.646 1.00 56.11H ATOM 3220 CA ALA 208 144.584 22.933 51.342 1.00 41.51 H ATOM 3221 CBALA 208 145.043 23.899 50.261 1.00 34.52 H ATOM 3222 C ALA 208 145.39721.638 51.269 1.00 43.41 H ATOM 3223 O ALA 208 144.983 20.676 50.6151.00 51.37 H ATOM 3224 N SER 209 146.550 21.615 51.933 1.00 47.62 H ATOM3225 CA SER 209 147.396 20.424 51.923 1.00 48.72 H ATOM 3226 CB SER 209148.852 20.811 51.634 1.00 44.67 H ATOM 3227 OG SER 209 149.387 21.64252.650 1.00 55.80 H ATOM 3228 C SER 209 147.304 19.643 53.236 1.00 53.29H ATOM 3229 O SER 209 147.973 18.624 53.411 1.00 52.35 H ATOM 3230 N SER210 146.462 20.119 54.148 1.00 71.17 H ATOM 3231 CA SER 210 146.26919.475 55.444 1.00 82.90 H ATOM 3232 CB SER 210 145.509 18.158 55.2771.00 90.95 H ATOM 3233 OG SER 210 144.137 18.333 55.578 1.00 100.00 HATOM 3234 C SER 210 147.579 19.213 56.169 1.00 79.59 H ATOM 3235 O SER210 147.702 18.234 56.908 1.00 80.94 H ATOM 3236 N THR 211 148.55520.088 55.950 1.00 73.59 H ATOM 3237 CA THR 211 149.854 19.964 56.5941.00 75.14 H ATOM 3238 CB THR 211 151.027 20.111 55.599 1.00 76.28 HATOM 3239 OG1 THR 211 150.990 21.414 55.008 1.00 73.09 H ATOM 3240 CG2THR 211 150.950 19.062 54.510 1.00 89.67 H ATOM 3241 C THR 211 150.02421.051 57.643 1.00 74.45 H ATOM 3242 O THR 211 149.536 22.172 57.4911.00 76.65 H ATOM 3243 N LYS 212 150.711 20.699 58.722 1.00 69.16 H ATOM3244 CA LYS 212 150.988 21.645 59.783 1.00 61.67 H ATOM 3245 CB LYS 212150.041 21.450 60.967 1.00 73.38 H ATOM 3246 CG LYS 212 150.718 21.61262.317 1.00 89.85 H ATOM 3247 CD LYS 212 150.006 22.614 63.192 1.0099.99 H ATOM 3248 CE LYS 212 150.452 22.449 64.637 1.00 97.52 H ATOM3249 NZ LYS 212 150.308 23.707 65.419 1.00 95.76 H ATOM 3250 C LYS 212152.413 21.344 60.198 1.00 51.18 H ATOM 3251 O LYS 212 152.730 20.22260.594 1.00 46.57 H ATOM 3252 N VAL 213 153.275 22.349 60.081 1.00 47.52H ATOM 3253 CA VAL 213 154.672 22.201 60.434 1.00 33.06 H ATOM 3254 CBVAL 213 155.593 22.651 59.282 1.00 35.68 H ATOM 3255 CG1 VAL 213 157.00222.136 59.511 1.00 51.97 H ATOM 3256 CG2 VAL 213 155.047 22.154 57.9481.00 53.21 H ATOM 3257 C VAL 213 155.011 23.028 61.661 1.00 30.06 H ATOM3258 O VAL 213 154.324 23.996 61.991 1.00 34.34 H ATOM 3259 N ASP 214156.071 22.625 62.343 1.00 35.26 H ATOM 3260 CA ASP 214 156.541 23.32563.523 1.00 39.19 H ATOM 3261 CB ASP 214 156.228 22.526 64.791 1.0058.41 H ATOM 3262 CG ASP 214 154.791 22.709 65.259 1.00 63.54 H ATOM3263 OD1 ASP 214 154.397 23.857 65.553 1.00 65.10 H ATOM 3264 OD2 ASP214 154.059 21.699 65.336 1.00 80.45 H ATOM 3265 C ASP 214 158.04623.454 63.342 1.00 37.64 H ATOM 3266 O ASP 214 158.725 22.489 62.9831.00 41.00 H ATOM 3267 N LYS 215 158.564 24.652 63.564 1.00 34.85 H ATOM3268 CA LYS 215 159.985 24.877 63.410 1.00 35.83 H ATOM 3269 CB LYS 215160.261 25.637 62.112 1.00 37.75 H ATOM 3270 CG LYS 215 161.248 24.94861.191 1.00 59.89 H ATOM 3271 CD LYS 215 160.536 23.992 60.251 1.0059.31 H ATOM 3272 CE LYS 215 161.386 22.766 59.964 1.00 53.98 H ATOM3273 NZ LYS 215 160.573 21.613 59.475 1.00 65.45 H ATOM 3274 C LYS 215160.515 25.659 64.588 1.00 38.73 H ATOM 3275 O LYS 215 160.030 26.74564.904 1.00 53.68 H ATOM 3276 N LYS 216 161.511 25.084 65.244 1.00 48.31H ATOM 3277 CA LYS 216 162.141 25.714 66.389 1.00 47.35 H ATOM 3278 CBLYS 216 162.649 24.641 67.357 1.00 48.55 H ATOM 3279 CG LYS 216 162.04323.256 67.119 1.00 74.84 H ATOM 3280 CD LYS 216 162.861 22.156 67.7931.00 85.40 H ATOM 3281 CE LYS 216 162.592 22.085 69.296 1.00 94.97 HATOM 3282 NZ LYS 216 162.133 20.733 69.738 1.00 92.19 H ATOM 3283 C LYS216 163.305 26.543 65.860 1.00 46.24 H ATOM 3284 O LYS 216 163.89826.203 64.838 1.00 34.41 H ATOM 3285 N ILE 217 163.622 27.637 66.5411.00 41.02 H ATOM 3286 CA ILE 217 164.719 28.497 66.116 1.00 44.97 HATOM 3287 CB ILE 217 164.273 29.978 66.046 1.00 41.86 H ATOM 3288 CG2ILE 217 165.094 30.716 64.991 1.00 44.48 H ATOM 3289 CG1 ILE 217 162.76830.063 65.749 1.00 33.66 H ATOM 3290 CD1 ILE 217 162.407 30.038 64.2701.00 17.95 H ATOM 3291 C ILE 217 165.890 28.363 67.094 1.00 47.47 H ATOM3292 O ILE 217 165.690 28.400 68.310 1.00 43.27 H ATOM 3293 N VAL 218167.103 28.219 66.559 1.00 50.40 H ATOM 3294 CA VAL 218 168.301 28.05467.394 1.00 50.95 H ATOM 3295 CB VAL 218 169.003 26.705 67.084 1.0057.08 H ATOM 3296 CG1 VAL 218 167.974 25.686 66.599 1.00 55.44 H ATOM3297 CG2 VAL 218 170.105 26.903 66.037 1.00 73.39 H ATOM 3298 C VAL 218169.344 29.175 67.289 1.00 41.93 H ATOM 3299 O VAL 218 170.001 29.45868.317 1.00 46.19 H ATOM 3300 OT VAL 218 170.011 29.390 69.119 1.0052.14 H ATOM 3301 CB LYS 2 95.854 32.588 47.685 1.00 42.79 C ATOM 3302CG LYS 2 95.825 31.435 46.699 1.00 53.80 C ATOM 3303 CD LYS 2 94.40730.925 46.486 1.00 62.43 C ATOM 3304 CE LYS 2 94.373 29.740 45.531 1.0062.95 C ATOM 3305 NZ LYS 2 94.065 28.460 46.232 1.00 60.28 C ATOM 3306 CLYS 2 94.693 33.099 49.840 1.00 46.91 C ATOM 3307 O LYS 2 93.606 32.67350.239 1.00 58.66 C ATOM 3308 N LYS 2 95.259 30.740 49.234 1.00 34.71 CATOM 3309 CA LYS 2 95.690 32.169 49.150 1.00 47.34 C ATOM 3310 N ILE 395.072 34.368 49.975 1.00 39.77 C ATOM 3311 CA ILE 3 94.226 35.37250.621 1.00 41.61 C ATOM 3312 CB ILE 3 95.062 36.456 51.331 1.00 43.12 CATOM 3313 CG2 ILE 3 94.142 37.421 52.074 1.00 41.52 C ATOM 3314 CG1 ILE3 96.056 35.818 52.301 1.00 48.11 C ATOM 3315 CD1 ILE 3 97.282 36.67252.556 1.00 29.73 C ATOM 3316 C ILE 3 93.346 36.101 49.623 1.00 38.09 CATOM 3317 O ILE 3 93.645 36.147 48.437 1.00 41.06 C ATOM 3318 N LEU 492.266 36.686 50.124 1.00 41.63 C ATOM 3319 CA LEU 4 91.349 37.43949.287 1.00 47.21 C ATOM 3320 CB LEU 4 89.906 37.026 49.581 1.00 47.90 CATOM 3321 CG LEU 4 89.389 35.782 48.852 1.00 49.25 C ATOM 3322 CD1 LEU 489.552 35.970 47.351 1.00 37.24 C ATOM 3323 CD2 LEU 4 90.138 34.54349.324 1.00 54.80 C ATOM 3324 C LEU 4 91.536 38.927 49.569 1.00 48.08 CATOM 3325 O LEU 4 91.868 39.322 50.695 1.00 31.46 C ATOM 3326 N VAL 591.326 39.743 48.538 1.00 43.17 C ATOM 3327 CA VAL 5 91.469 41.19048.660 1.00 33.62 C ATOM 3328 CB VAL 5 92.806 41.673 48.047 1.00 32.71 CATOM 3329 CG1 VAL 5 93.315 42.880 48.803 1.00 22.52 C ATOM 3330 CG2 VAL5 93.836 40.559 48.081 1.00 29.21 C ATOM 3331 C VAL 5 90.325 41.91147.945 1.00 36.98 C ATOM 3332 O VAL 5 90.028 41.628 46.788 1.00 50.54 CATOM 3333 N LYS 6 89.689 42.844 48.640 1.00 39.70 C ATOM 3334 CA LYS 688.593 43.622 48.068 1.00 41.54 C ATOM 3335 CB LYS 6 87.299 43.37848.857 1.00 38.28 C ATOM 3336 CG LYS 6 86.443 42.233 48.341 1.00 48.43 CATOM 3337 CD LYS 6 85.343 41.841 49.339 1.00 58.62 C ATOM 3338 CE LYS 685.905 41.158 50.593 1.00 47.34 C ATOM 3339 NZ LYS 6 86.645 39.89350.281 1.00 51.47 C ATOM 3340 C LYS 6 88.957 45.108 48.147 1.00 32.95 CATOM 3341 O LYS 6 89.265 45.614 49.222 1.00 43.88 C ATOM 3342 N GLN 788.926 45.805 47.021 1.00 30.15 C ATOM 3343 CA GLN 7 89.258 47.22447.022 1.00 39.07 C ATOM 3344 CB GLN 7 90.631 47.449 46.395 1.00 44.76 CATOM 3345 CG GLN 7 91.355 46.178 46.002 1.00 48.85 C ATOM 3346 CD GLN 792.745 46.462 45.467 1.00 53.95 C ATOM 3347 OE1 GLN 7 93.575 45.56045.334 1.00 50.12 C ATOM 3348 NE2 GLN 7 93.007 47.730 45.155 1.00 35.66C ATOM 3349 C GLN 7 88.230 48.035 46.262 1.00 36.89 C ATOM 3350 O GLN 787.567 47.514 45.370 1.00 42.41 C ATOM 3351 N SER 8 88.095 49.307 46.6231.00 34.89 C ATOM 3352 CA SER 8 87.148 50.178 45.940 1.00 33.64 C ATOM3353 CB SER 8 87.247 51.613 46.475 1.00 39.10 C ATOM 3354 OG SER 886.259 51.882 47.465 1.00 33.65 C ATOM 3355 C SER 8 87.564 50.141 44.4811.00 36.57 C ATOM 3356 O SER 8 88.751 50.012 44.180 1.00 34.54 C ATOM3357 N PRO 9 86.596 50.239 43.553 1.00 45.61 C ATOM 3358 CD PRO 9 85.14650.390 43.755 1.00 42.54 C ATOM 3359 CA PRO 9 86.946 50.207 42.128 1.0042.76 C ATOM 3360 CB PRO 9 85.594 50.325 41.411 1.00 35.11 C ATOM 3361CG PRO 9 84.577 49.955 42.428 1.00 29.77 C ATOM 3362 C PRO 9 87.87951.370 41.813 1.00 45.27 C ATOM 3363 O PRO 9 88.929 51.194 41.190 1.0049.59 C ATOM 3364 N MET 10 87.486 52.561 42.254 1.00 34.14 C ATOM 3365CA MET 10 88.292 53.749 42.041 1.00 36.35 C ATOM 3366 CB MET 10 87.97554.374 40.674 1.00 51.67 C ATOM 3367 CG MET 10 87.958 55.908 40.628 1.0045.29 C ATOM 3368 SD MET 10 86.852 56.596 39.357 1.00 41.23 C ATOM 3369CE MET 10 88.067 57.323 38.239 1.00 36.20 C ATOM 3370 C MET 10 88.03154.730 43.172 1.00 31.46 C ATOM 3371 O MET 10 87.032 54.629 43.882 1.0022.69 C ATOM 3372 N LEU 11 88.962 55.661 43.344 1.00 34.82 C ATOM 3373CA LEU 11 88.890 56.669 44.385 1.00 23.35 C ATOM 3374 CB LEU 11 89.82656.264 45.530 1.00 20.95 C ATOM 3375 CG LEU 11 89.592 54.804 45.946 1.0018.02 C ATOM 3376 CD1 LEU 11 90.765 54.235 46.696 1.00 5.04 C ATOM 3377CD2 LEU 11 88.339 54.745 46.785 1.00 31.87 C ATOM 3378 C LEU 11 89.30658.008 43.789 1.00 22.25 C ATOM 3379 O LEU 11 90.294 58.093 43.061 1.0023.22 C ATOM 3380 N VAL 12 88.540 59.051 44.075 1.00 23.39 C ATOM 3381CA VAL 12 88.871 60.365 43.551 1.00 25.58 C ATOM 3382 CB VAL 12 87.61061.166 43.174 1.00 37.83 C ATOM 3383 CG1 VAL 12 88.008 62.509 42.5851.00 20.17 C ATOM 3384 CG2 VAL 12 86.771 60.387 42.173 1.00 25.83 C ATOM3385 C VAL 12 89.655 61.133 44.603 1.00 30.45 C ATOM 3386 O VAL 1289.106 61.605 45.597 1.00 47.50 C ATOM 3387 N ALA 13 90.955 61.24644.379 1.00 37.89 C ATOM 3388 CA ALA 13 91.820 61.943 45.307 1.00 26.16C ATOM 3389 CB ALA 13 93.203 62.091 44.706 1.00 30.65 C ATOM 3390 C ALA13 91.241 63.309 45.615 1.00 33.79 C ATOM 3391 O ALA 13 90.816 64.02844.711 1.00 39.57 C ATOM 3392 N TYR 14 91.206 63.657 46.895 1.00 39.35 CATOM 3393 CA TYR 14 90.711 64.959 47.306 1.00 37.04 C ATOM 3394 CB TYR14 89.660 64.835 48.410 1.00 33.79 C ATOM 3395 CG TYR 14 89.555 66.11049.213 1.00 48.57 C ATOM 3396 CD1 TYR 14 90.182 66.233 50.458 1.00 50.52C ATOM 3397 CE1 TYR 14 90.178 67.444 51.148 1.00 54.90 C ATOM 3398 CD2TYR 14 88.913 67.231 48.685 1.00 44.13 C ATOM 3399 CE2 TYR 14 88.90468.441 49.364 1.00 49.92 C ATOM 3400 CZ TYR 14 89.538 68.545 50.590 1.0058.83 C ATOM 3401 OH TYR 14 89.536 69.761 51.240 1.00 69.05 C ATOM 3402C TYR 14 91.884 65.765 47.846 1.00 39.91 C ATOM 3403 O TYR 14 92.62665.283 48.703 1.00 36.85 C ATOM 3404 N ASP 15 92.041 66.992 47.359 1.0034.60 C ATOM 3405 CA ASP 15 93.131 67.838 47.820 1.00 35.97 C ATOM 3406CB ASP 15 92.958 68.141 49.307 1.00 54.21 C ATOM 3407 CG ASP 15 92.60569.588 49.573 1.00 74.11 C ATOM 3408 OD1 ASP 15 92.300 70.322 48.6041.00 77.84 C ATOM 3409 OD2 ASP 15 92.634 69.985 50.758 1.00 75.11 C ATOM3410 C ASP 15 94.448 67.112 47.603 1.00 33.47 C ATOM 3411 O ASP 1595.389 67.265 48.380 1.00 34.49 C ATOM 3412 N ASN 16 94.499 66.30946.543 1.00 44.74 C ATOM 3413 CA ASN 16 95.683 65.534 46.196 1.00 48.87C ATOM 3414 CB ASN 16 96.898 66.457 46.053 1.00 62.07 C ATOM 3415 CG ASN16 96.840 67.292 44.793 1.00 64.94 C ATOM 3416 OD1 ASN 16 96.213 68.35544.760 1.00 66.06 C ATOM 3417 ND2 ASN 16 97.497 66.816 43.742 1.00 73.29C ATOM 3418 C ASN 16 95.983 64.449 47.225 1.00 48.29 C ATOM 3419 O ASN16 97.148 64.096 47.451 1.00 38.81 C ATOM 3420 N ALA 17 94.923 63.92347.835 1.00 34.08 C ATOM 3421 CA ALA 17 95.055 62.867 48.840 1.00 29.57C ATOM 3422 CB ALA 17 94.996 63.459 50.237 1.00 33.14 C ATOM 3423 C ALA17 93.955 61.832 48.667 1.00 29.96 C ATOM 3424 O ALA 17 92.931 62.10748.049 1.00 32.16 C ATOM 3425 N VAL 18 94.169 60.642 49.213 1.00 28.09 CATOM 3426 CA VAL 18 93.188 59.568 49.111 1.00 30.54 C ATOM 3427 CB VAL18 93.339 58.824 47.767 1.00 15.07 C ATOM 3428 CG1 VAL 18 94.517 57.88947.829 1.00 23.83 C ATOM 3429 CG2 VAL 18 92.086 58.054 47.448 1.00 16.29C ATOM 3430 C VAL 18 93.356 58.575 50.266 1.00 33.43 C ATOM 3431 O VAL18 94.314 58.660 51.029 1.00 28.92 C ATOM 3432 N ASN 19 92.419 57.64350.398 1.00 30.17 C ATOM 3433 CA ASN 19 92.496 56.642 51.456 1.00 28.35C ATOM 3434 CB ASN 19 91.468 56.959 52.545 1.00 37.98 C ATOM 3435 CG ASN19 92.105 57.540 53.794 1.00 36.06 C ATOM 3436 OD1 ASN 19 92.991 56.92354.385 1.00 36.77 C ATOM 3437 ND2 ASN 19 91.659 58.732 54.205 1.00 27.02C ATOM 3438 C ASN 19 92.241 55.260 50.867 1.00 28.23 C ATOM 3439 O ASN19 91.113 54.941 50.502 1.00 25.92 C ATOM 3440 N LEU 20 93.291 54.44650.781 1.00 37.11 C ATOM 3441 CA LEU 20 93.192 53.097 50.204 1.00 39.33C ATOM 3442 CB LEU 20 94.382 52.830 49.284 1.00 52.73 C ATOM 3443 CG LEU20 94.409 53.492 47.913 1.00 59.34 C ATOM 3444 CD1 LEU 20 95.375 54.65747.953 1.00 54.35 C ATOM 3445 CD2 LEU 20 94.826 52.478 46.855 1.00 58.17C ATOM 3446 C LEU 20 93.122 51.946 51.202 1.00 24.88 C ATOM 3447 O LEU20 94.141 51.349 51.532 1.00 27.29 C ATOM 3448 N SER 21 91.926 51.61951.670 1.00 24.25 C ATOM 3449 CA SER 21 91.795 50.515 52.601 1.00 32.93C ATOM 3450 CB SER 21 90.474 50.603 53.357 1.00 22.84 C ATOM 3451 OG SER21 90.626 50.123 54.682 1.00 42.82 C ATOM 3452 C SER 21 91.834 49.25051.760 1.00 36.87 C ATOM 3453 O SER 21 91.633 49.308 50.551 1.00 50.83 CATOM 3454 N CYS 22 92.098 48.109 52.384 1.00 41.84 C ATOM 3455 CA CYS 2292.142 46.858 51.636 1.00 34.97 C ATOM 3456 C CYS 22 91.519 45.72852.420 1.00 29.54 C ATOM 3457 O CYS 22 92.086 45.250 53.400 1.00 31.51 CATOM 3458 CB CYS 22 93.578 46.504 51.294 1.00 44.42 C ATOM 3459 SG CYS22 94.382 47.674 50.162 1.00 54.15 C ATOM 3460 N LYS 23 90.339 45.31251.983 1.00 9.94 C ATOM 3461 CA LYS 23 89.630 44.234 52.639 1.00 27.48 CATOM 3462 CB LYS 23 88.196 44.149 52.099 1.00 45.60 C ATOM 3463 CG LYS23 87.083 44.123 53.160 1.00 46.91 C ATOM 3464 CD LYS 23 85.742 44.57252.558 1.00 49.03 C ATOM 3465 CE LYS 23 84.537 43.911 53.219 1.00 44.60C ATOM 3466 NZ LYS 23 83.321 43.966 52.347 1.00 31.56 C ATOM 3467 C LYS23 90.373 42.933 52.354 1.00 28.04 C ATOM 3468 O LYS 23 90.727 42.65151.209 1.00 27.74 C ATOM 3469 N TYR 24 90.616 42.154 53.404 1.00 48.19 CATOM 3470 CA TYR 24 91.305 40.869 53.279 1.00 51.31 C ATOM 3471 CB TYR24 92.585 40.864 54.121 1.00 33.41 C ATOM 3472 CG TYR 24 93.582 41.91453.708 1.00 28.69 C ATOM 3473 CD1 TYR 24 94.190 41.867 52.454 1.00 41.70C ATOM 3474 CE1 TYR 24 95.089 42.846 52.049 1.00 20.51 C ATOM 3475 CD2TYR 24 93.901 42.971 54.554 1.00 29.67 C ATOM 3476 CE2 TYR 24 94.80343.960 54.159 1.00 21.03 C ATOM 3477 CZ TYR 24 95.389 43.889 52.905 1.0018.10 C ATOM 3478 OH TYR 24 96.263 44.862 52.495 1.00 37.76 C ATOM 3479C TYR 24 90.403 39.736 53.749 1.00 47.73 C ATOM 3480 O TYR 24 89.26439.957 54.151 1.00 49.24 C ATOM 3481 N SER 25 90.920 38.518 53.690 1.0057.38 C ATOM 3482 CA SER 25 90.175 37.353 54.146 1.00 60.28 C ATOM 3483CB SER 25 89.888 36.407 52.975 1.00 59.25 C ATOM 3484 OG SER 25 91.06836.142 52.238 1.00 69.79 C ATOM 3485 C SER 25 91.076 36.668 55.164 1.0054.52 C ATOM 3486 O SER 25 91.290 35.455 55.108 1.00 60.55 C ATOM 3487 NTYR 26 91.606 37.458 56.096 1.00 43.18 C ATOM 3488 CA TYR 26 92.51636.933 57.101 1.00 31.88 C ATOM 3489 CB TYR 26 93.949 36.995 56.566 1.0026.62 C ATOM 3490 CG TYR 26 94.845 35.878 57.060 1.00 37.63 C ATOM 3491CD1 TYR 26 95.606 36.026 58.220 1.00 43.69 C ATOM 3492 CE1 TYR 26 96.44335.003 58.673 1.00 50.14 C ATOM 3493 CD2 TYR 26 94.941 34.677 56.3631.00 43.23 C ATOM 3494 CE2 TYR 26 95.776 33.646 56.809 1.00 62.28 C ATOM3495 CZ TYR 26 96.524 33.816 57.964 1.00 56.86 C ATOM 3496 OH TYR 2697.352 32.801 58.404 1.00 54.48 C ATOM 3497 C TYR 26 92.455 37.63758.451 1.00 30.34 C ATOM 3498 O TYR 26 92.275 38.851 58.518 1.00 12.16 CATOM 3499 N ASN 27 92.626 36.832 59.504 1.00 35.16 C ATOM 3500 CA ASN 2792.639 37.230 60.920 1.00 43.53 C ATOM 3501 CB ASN 27 94.092 37.46161.380 1.00 44.74 C ATOM 3502 CG ASN 27 94.595 38.869 61.095 1.00 54.71C ATOM 3503 OD1 ASN 27 93.890 39.691 60.503 1.00 49.86 C ATOM 3504 ND2ASN 27 95.833 39.153 61.521 1.00 38.35 C ATOM 3505 C ASN 27 91.76038.379 61.413 1.00 52.07 C ATOM 3506 O ASN 27 91.255 39.159 60.582 1.0064.58 C ATOM 3507 OT ASN 27 91.583 38.478 62.651 1.00 38.64 C ATOM 3508CB SER 30 98.029 39.101 66.273 1.00 38.67 C ATOM 3509 OG SER 30 99.16439.940 66.371 1.00 28.74 C ATOM 3510 C SER 30 99.198 37.741 64.527 1.0048.62 C ATOM 3511 O SER 30 99.534 36.748 65.163 1.00 42.19 C ATOM 3512 NSER 30 96.758 37.537 64.847 1.00 40.61 C ATOM 3513 CA SER 30 97.91038.480 64.881 1.00 46.89 C ATOM 3514 N ARG 31 99.906 38.226 63.505 1.0052.76 C ATOM 3515 CA ARG 31 101.156 37.606 63.056 1.00 48.62 C ATOM 3516CB ARG 31 100.864 36.509 62.014 1.00 48.21 C ATOM 3517 CG ARG 31 99.39036.316 61.654 1.00 45.83 C ATOM 3518 CD ARG 31 99.123 34.913 61.098 1.0045.48 C ATOM 3519 NE ARG 31 98.202 34.159 61.946 1.00 56.80 C ATOM 3520CZ ARG 31 97.819 32.904 61.723 1.00 60.88 C ATOM 3521 NH1 ARG 31 98.27332.238 60.666 1.00 53.06 C ATOM 3522 NH2 ARG 31 96.980 32.312 62.5651.00 65.41 C ATOM 3523 C ARG 31 102.140 38.620 62.457 1.00 42.34 C ATOM3524 O ARG 31 102.024 39.821 62.696 1.00 37.05 C ATOM 3525 N GLU 32103.109 38.119 61.688 1.00 33.63 C ATOM 3526 CA GLU 32 104.114 38.95961.035 1.00 28.55 C ATOM 3527 CB GLU 32 105.510 38.366 61.211 1.00 35.76C ATOM 3528 CG GLU 32 106.639 39.289 60.762 1.00 44.46 C ATOM 3529 CDGLU 32 107.993 38.584 60.704 1.00 47.94 C ATOM 3530 OE1 GLU 32 108.18337.588 61.436 1.00 50.54 C ATOM 3531 OE2 GLU 32 108.870 39.024 59.9311.00 43.16 C ATOM 3532 C GLU 32 103.777 39.047 59.550 1.00 32.96 C ATOM3533 O GLU 32 103.876 38.061 58.815 1.00 19.87 C ATOM 3534 N PHE 33103.394 40.244 59.122 1.00 41.41 C ATOM 3535 CA PHE 33 102.989 40.48857.747 1.00 35.05 C ATOM 3536 CB PHE 33 101.494 40.691 57.715 1.00 26.59C ATOM 3537 CG PHE 33 101.065 41.944 58.408 1.00 24.17 C ATOM 3538 CD1PHE 33 101.369 43.192 57.861 1.00 23.40 C ATOM 3539 CD2 PHE 33 100.37441.889 59.611 1.00 15.64 C ATOM 3540 CE1 PHE 33 100.989 44.367 58.4961.00 28.02 C ATOM 3541 CE2 PHE 33 99.984 43.063 60.260 1.00 24.23 C ATOM3542 CZ PHE 33 100.292 44.304 59.703 1.00 31.35 C ATOM 3543 C PHE 33103.640 41.716 57.121 1.00 34.54 C ATOM 3544 O PHE 33 104.134 42.61357.813 1.00 21.74 C ATOM 3545 N ARG 34 103.564 41.771 55.797 1.00 28.28C ATOM 3546 CA ARG 34 104.145 42.864 55.045 1.00 21.13 C ATOM 3547 CBARG 34 105.469 42.413 54.437 1.00 12.61 C ATOM 3548 CG ARG 34 106.18943.483 53.643 1.00 19.39 C ATOM 3549 CD ARG 34 106.709 42.916 52.3391.00 18.93 C ATOM 3550 NE ARG 34 108.026 43.440 51.995 1.00 36.83 C ATOM3551 CZ ARG 34 108.251 44.344 51.045 1.00 40.83 C ATOM 3552 NH1 ARG 34107.239 44.825 50.340 1.00 45.97 C ATOM 3553 NH2 ARG 34 109.490 44.75250.785 1.00 38.52 C ATOM 3554 C ARG 34 103.202 43.309 53.940 1.00 32.95C ATOM 3555 O ARG 34 103.239 42.779 52.831 1.00 41.93 C ATOM 3556 N ALA35 102.362 44.294 54.239 1.00 34.63 C ATOM 3557 CA ALA 35 101.416 44.79853.251 1.00 26.09 C ATOM 3558 CB ALA 35 100.381 45.657 53.921 1.00 26.37C ATOM 3559 C ALA 35 102.134 45.599 52.173 1.00 28.19 C ATOM 3560 O ALA35 103.201 46.163 52.414 1.00 19.20 C ATOM 3561 N SER 36 101.545 45.64150.980 1.00 36.87 C ATOM 3562 CA SER 36 102.136 46.374 49.863 1.00 39.36C ATOM 3563 CB SER 36 103.172 45.488 49.160 1.00 51.82 C ATOM 3564 OGSER 36 103.705 44.516 50.050 1.00 62.97 C ATOM 3565 C SER 36 101.09946.886 48.847 1.00 32.13 C ATOM 3566 O SER 36 100.092 46.238 48.575 1.0028.44 C ATOM 3567 N LEU 37 101.364 48.069 48.304 1.00 34.51 C ATOM 3568CA LEU 37 100.496 48.705 47.320 1.00 31.61 C ATOM 3569 CB LEU 37 99.89749.987 47.895 1.00 36.64 C ATOM 3570 CG LEU 37 98.943 50.801 47.023 1.0021.97 C ATOM 3571 CD1 LEU 37 97.533 50.720 47.578 1.00 21.64 C ATOM 3572CD2 LEU 37 99.412 52.231 46.991 1.00 26.43 C ATOM 3573 C LEU 37 101.39349.042 46.148 1.00 28.60 C ATOM 3574 O LEU 37 102.341 49.811 46.295 1.0035.19 C ATOM 3575 N HIS 38 101.112 48.454 44.993 1.00 22.34 C ATOM 3576CA HIS 38 101.935 48.694 43.816 1.00 21.00 C ATOM 3577 CB HIS 38 102.41847.370 43.232 1.00 29.80 C ATOM 3578 CG HIS 38 103.038 46.464 44.2481.00 41.65 C ATOM 3579 CD2 HIS 38 104.324 46.102 44.458 1.00 36.66 CATOM 3580 ND1 HIS 38 102.296 45.819 45.218 1.00 51.35 C ATOM 3581 CE1HIS 38 103.102 45.102 45.978 1.00 45.79 C ATOM 3582 NE2 HIS 38 104.33745.256 45.539 1.00 50.11 C ATOM 3583 C HIS 38 101.138 49.458 42.794 1.0020.21 C ATOM 3584 O HIS 38 99.935 49.263 42.679 1.00 24.62 C ATOM 3585 NLYS 39 101.807 50.334 42.055 1.00 17.62 C ATOM 3586 CA LYS 39 101.13551.154 41.065 1.00 28.42 C ATOM 3587 CB LYS 39 101.458 52.627 41.3341.00 33.83 C ATOM 3588 CG LYS 39 100.938 53.579 40.281 1.00 47.76 C ATOM3589 CD LYS 39 102.046 54.496 39.767 1.00 56.33 C ATOM 3590 CE LYS 39101.922 54.727 38.264 1.00 52.05 C ATOM 3591 NZ LYS 39 101.573 56.13337.920 1.00 39.26 C ATOM 3592 C LYS 39 101.527 50.784 39.644 1.00 37.38C ATOM 3593 O LYS 39 102.715 50.697 39.323 1.00 38.26 C ATOM 3594 N GLY40 100.528 50.559 38.793 1.00 33.72 C ATOM 3595 CA GLY 40 100.819 50.23037.412 1.00 30.90 C ATOM 3596 C GLY 40 100.183 48.957 36.913 1.00 33.65C ATOM 3597 O GLY 40 100.025 47.997 37.665 1.00 26.93 C ATOM 3598 N LEU41 99.819 48.962 35.634 1.00 48.13 C ATOM 3599 CA LEU 41 99.196 47.80934.988 1.00 43.35 C ATOM 3600 CB LEU 41 98.598 48.211 33.633 1.00 41.37C ATOM 3601 CG LEU 41 97.256 47.613 33.178 1.00 50.66 C ATOM 3602 CD1LEU 41 96.622 46.783 34.283 1.00 41.32 C ATOM 3603 CD2 LEU 41 96.32448.742 32.756 1.00 36.16 C ATOM 3604 C LEU 41 100.270 46.758 34.791 1.0044.95 C ATOM 3605 O LEU 41 99.976 45.585 34.577 1.00 48.29 C ATOM 3606 NASP 42 101.522 47.197 34.841 1.00 51.52 C ATOM 3607 CA ASP 42 102.64846.285 34.713 1.00 53.82 C ATOM 3608 CB ASP 42 103.666 46.804 33.6901.00 44.60 C ATOM 3609 CG ASP 42 104.352 48.090 34.125 1.00 49.55 C ATOM3610 OD1 ASP 42 103.874 48.765 35.063 1.00 40.26 C ATOM 3611 OD2 ASP 42105.385 48.427 33.510 1.00 48.33 C ATOM 3612 C ASP 42 103.249 46.24136.106 1.00 57.65 C ATOM 3613 O ASP 42 104.394 45.836 36.298 1.00 57.61C ATOM 3614 N SER 43 102.433 46.679 37.065 1.00 57.54 C ATOM 3615 CA SER43 102.767 46.741 38.486 1.00 56.75 C ATOM 3616 CB SER 43 102.299 45.46739.177 1.00 40.78 C ATOM 3617 OG SER 43 100.896 45.502 39.359 1.00 32.86C ATOM 3618 C SER 43 104.234 46.995 38.803 1.00 59.27 C ATOM 3619 O SER43 104.795 46.413 39.732 1.00 57.66 C ATOM 3620 N ALA 44 104.847 47.88438.033 1.00 54.64 C ATOM 3621 CA ALA 44 106.244 48.215 38.224 1.00 41.67C ATOM 3622 CB ALA 44 106.654 49.295 37.239 1.00 41.38 C ATOM 3623 C ALA44 106.514 48.679 39.646 1.00 42.01 C ATOM 3624 O ALA 44 107.073 47.93540.449 1.00 48.39 C ATOM 3625 N VAL 45 106.083 49.904 39.943 1.00 40.52C ATOM 3626 CA VAL 45 106.290 50.563 41.239 1.00 34.97 C ATOM 3627 CBVAL 45 106.036 52.073 41.094 1.00 27.80 C ATOM 3628 CG1 VAL 45 106.12652.753 42.441 1.00 32.20 C ATOM 3629 CG2 VAL 45 107.036 52.674 40.1261.00 35.71 C ATOM 3630 C VAL 45 105.526 50.100 42.489 1.00 35.47 C ATOM3631 O VAL 45 104.307 49.956 42.462 1.00 45.80 C ATOM 3632 N GLU 46106.259 49.893 43.585 1.00 39.87 C ATOM 3633 CA GLU 46 105.666 49.50344.868 1.00 41.71 C ATOM 3634 CB GLU 46 106.546 48.488 45.615 1.00 50.85C ATOM 3635 CG GLU 46 106.163 48.251 47.094 1.00 34.64 C ATOM 3636 CDGLU 46 106.638 46.895 47.637 1.00 57.55 C ATOM 3637 OE1 GLU 46 107.86946.712 47.809 1.00 47.66 C ATOM 3638 OE2 GLU 46 105.783 46.014 47.8931.00 29.27 C ATOM 3639 C GLU 46 105.575 50.791 45.680 1.00 37.48 C ATOM3640 O GLU 46 106.530 51.189 46.338 1.00 43.67 C ATOM 3641 N VAL 47104.418 51.433 45.614 1.00 25.13 C ATOM 3642 CA VAL 47 104.173 52.69246.297 1.00 15.83 C ATOM 3643 CB VAL 47 102.736 53.146 46.037 1.00 19.37C ATOM 3644 CG1 VAL 47 102.428 54.405 46.826 1.00 28.90 C ATOM 3645 CG2VAL 47 102.543 53.374 44.547 1.00 26.86 C ATOM 3646 C VAL 47 104.43752.717 47.800 1.00 29.17 C ATOM 3647 O VAL 47 105.070 53.641 48.310 1.0031.30 C ATOM 3648 N CYS 48 103.952 51.709 48.512 1.00 40.43 C ATOM 3649CA CYS 48 104.142 51.665 49.955 1.00 37.33 C ATOM 3650 C CYS 48 104.14550.246 50.495 1.00 27.79 C ATOM 3651 O CYS 48 103.618 49.331 49.864 1.0020.24 C ATOM 3652 CB CYS 48 103.035 52.459 50.644 1.00 57.93 C ATOM 3653SG CYS 48 103.314 52.822 52.407 1.00 69.94 C ATOM 3654 N VAL 49 104.74750.070 51.664 1.00 18.58 C ATOM 3655 CA VAL 49 104.802 48.764 52.2901.00 20.44 C ATOM 3656 CB VAL 49 106.139 48.030 51.966 1.00 27.56 C ATOM3657 CG1 VAL 49 107.042 48.931 51.131 1.00 18.34 C ATOM 3658 CG2 VAL 49106.851 47.592 53.249 1.00 17.63 C ATOM 3659 C VAL 49 104.654 48.92653.794 1.00 33.14 C ATOM 3660 O VAL 49 105.537 49.471 54.455 1.00 29.92C ATOM 3661 N VAL 50 103.523 48.467 54.324 1.00 41.12 C ATOM 3662 CA VAL50 103.255 48.539 55.759 1.00 44.37 C ATOM 3663 CB VAL 50 101.796 48.93256.031 1.00 48.04 C ATOM 3664 CG1 VAL 50 101.486 48.786 57.511 1.0045.70 C ATOM 3665 CG2 VAL 50 101.562 50.367 55.576 1.00 35.76 C ATOM3666 C VAL 50 103.553 47.176 56.385 1.00 38.20 C ATOM 3667 O VAL 50102.899 46.176 56.085 1.00 36.51 C ATOM 3668 N TYR 51 104.543 47.14657.264 1.00 25.60 C ATOM 3669 CA TYR 51 104.957 45.898 57.879 1.00 34.11C ATOM 3670 CB TYR 51 106.479 45.779 57.799 1.00 34.41 C ATOM 3671 CGTYR 51 107.060 44.744 58.724 1.00 31.19 C ATOM 3672 CD1 TYR 51 107.61745.109 59.944 1.00 38.77 C ATOM 3673 CE1 TYR 51 108.122 44.153 60.8221.00 44.07 C ATOM 3674 CD2 TYR 51 107.025 43.396 58.395 1.00 33.29 CATOM 3675 CE2 TYR 51 107.525 42.432 59.265 1.00 41.62 C ATOM 3676 CZ TYR51 108.068 42.818 60.477 1.00 39.08 C ATOM 3677 OH TYR 51 108.511 41.86761.364 1.00 47.15 C ATOM 3678 C TYR 51 104.507 45.729 59.315 1.00 33.68C ATOM 3679 O TYR 51 104.696 46.613 60.145 1.00 23.59 C ATOM 3680 N GLY52 103.927 44.571 59.603 1.00 29.01 C ATOM 3681 CA GLY 52 103.459 44.30060.947 1.00 30.05 C ATOM 3682 C GLY 52 104.067 43.041 61.527 1.00 39.73C ATOM 3683 O GLY 52 104.393 42.096 60.806 1.00 40.35 C ATOM 3684 N ASN53 104.224 43.032 62.843 1.00 46.66 C ATOM 3685 CA ASN 53 104.798 41.88863.537 1.00 57.56 C ATOM 3686 CB ASN 53 106.308 42.073 63.721 1.00 32.73C ATOM 3687 CG ASN 53 106.971 40.851 64.322 1.00 30.74 C ATOM 3688 OD1ASN 53 106.474 39.728 64.185 1.00 25.99 C ATOM 3689 ND2 ASN 53 108.09941.059 64.995 1.00 35.70 C ATOM 3690 C ASN 53 104.141 41.729 64.897 1.0063.15 C ATOM 3691 O ASN 53 104.709 42.114 65.918 1.00 67.81 C ATOM 3692N TYR 54 102.941 41.159 64.906 1.00 66.70 C ATOM 3693 CA TYR 54 102.21340.953 66.149 1.00 70.87 C ATOM 3694 CB TYR 54 103.004 40.008 67.0721.00 60.20 C ATOM 3695 CG TYR 54 103.185 38.604 66.521 1.00 54.18 C ATOM3696 CD1 TYR 54 104.380 38.215 65.923 1.00 49.72 C ATOM 3697 CE1 TYR 54104.538 36.931 65.393 1.00 51.50 C ATOM 3698 CD2 TYR 54 102.149 37.67266.579 1.00 63.25 C ATOM 3699 CE2 TYR 54 102.297 36.386 66.052 1.0053.22 C ATOM 3700 CZ TYR 54 103.489 36.025 65.461 1.00 51.26 C ATOM 3701OH TYR 54 103.625 34.761 64.931 1.00 41.36 C ATOM 3702 C TYR 54 102.01842.313 66.811 1.00 75.80 C ATOM 3703 O TYR 54 101.118 43.072 66.443 1.0070.77 C ATOM 3704 N SER 55 102.870 42.617 67.784 1.00 83.17 C ATOM 3705CA SER 55 102.805 43.892 68.483 1.00 89.21 C ATOM 3706 CB SER 55 103.37343.750 69.896 1.00 89.77 C ATOM 3707 OG SER 55 102.332 43.662 70.8531.00 90.69 C ATOM 3708 C SER 55 103.617 44.916 67.695 1.00 90.09 C ATOM3709 O SER 55 103.181 45.378 66.639 1.00 92.24 C ATOM 3710 N GLN 56104.796 45.251 68.218 1.00 90.35 C ATOM 3711 CA GLN 56 105.715 46.20767.600 1.00 93.42 C ATOM 3712 CB GLN 56 106.882 45.448 66.957 1.00 91.24C ATOM 3713 CG GLN 56 107.492 44.372 67.861 1.00 94.20 C ATOM 3714 CDGLN 56 108.069 43.192 67.088 1.00 99.99 C ATOM 3715 OE1 GLN 56 108.12842.066 67.595 1.00 87.06 C ATOM 3716 NE2 GLN 56 108.500 43.446 65.8541.00 99.99 C ATOM 3717 C GLN 56 105.040 47.121 66.572 1.00 96.11 C ATOM3718 O GLN 56 104.866 46.744 65.413 1.00 99.98 C ATOM 3719 N GLN 57104.670 48.323 67.013 1.00 90.45 C ATOM 3720 CA GLN 57 103.995 49.31866.177 1.00 83.45 C ATOM 3721 CB GLN 57 104.417 50.732 66.589 1.00 85.60C ATOM 3722 CG GLN 57 104.367 50.991 68.084 1.00 89.34 C ATOM 3723 CDGLN 57 105.744 50.966 68.718 1.00 87.16 C ATOM 3724 OE1 GLN 57 106.49751.937 68.635 1.00 76.99 C ATOM 3725 NE2 GLN 57 106.080 49.849 69.3551.00 90.12 C ATOM 3726 C GLN 57 104.185 49.175 64.669 1.00 80.35 C ATOM3727 O GLN 57 105.278 48.877 64.182 1.00 77.96 C ATOM 3728 N LEU 58103.095 49.401 63.943 1.00 76.47 C ATOM 3729 CA LEU 58 103.077 49.33162.487 1.00 61.35 C ATOM 3730 CB LEU 58 101.723 49.838 61.972 1.00 52.59C ATOM 3731 CG LEU 58 100.956 49.130 60.845 1.00 50.02 C ATOM 3732 CD1LEU 58 100.916 47.634 61.070 1.00 55.58 C ATOM 3733 CD2 LEU 58 99.53549.671 60.794 1.00 39.64 C ATOM 3734 C LEU 58 104.209 50.181 61.906 1.0054.39 C ATOM 3735 O LEU 58 104.387 51.345 62.284 1.00 51.09 C ATOM 3736N GLN 59 104.976 49.589 60.997 1.00 51.81 C ATOM 3737 CA GLN 59 106.08050.290 60.346 1.00 46.97 C ATOM 3738 CB GLN 59 107.394 49.516 60.5241.00 35.66 C ATOM 3739 CG GLN 59 108.469 50.260 61.308 1.00 32.42 C ATOM3740 CD GLN 59 109.327 49.340 62.171 1.00 53.43 C ATOM 3741 OE1 GLN 59110.198 49.799 62.912 1.00 50.95 C ATOM 3742 NE2 GLN 59 109.082 48.03762.079 1.00 46.72 C ATOM 3743 C GLN 59 105.748 50.417 58.859 1.00 43.43C ATOM 3744 O GLN 59 105.697 49.422 58.138 1.00 42.60 C ATOM 3745 N VAL60 105.505 51.645 58.412 1.00 40.75 C ATOM 3746 CA VAL 60 105.172 51.90757.018 1.00 39.80 C ATOM 3747 CB VAL 60 103.828 52.659 56.896 1.00 26.19C ATOM 3748 CG1 VAL 60 103.878 53.955 57.678 1.00 21.41 C ATOM 3749 CG2VAL 60 103.528 52.946 55.448 1.00 27.26 C ATOM 3750 C VAL 60 106.26752.745 56.371 1.00 41.05 C ATOM 3751 O VAL 60 106.733 53.727 56.950 1.0051.96 C ATOM 3752 N TYR 61 106.692 52.349 55.177 1.00 37.63 C ATOM 3753CA TYR 61 107.731 53.086 54.477 1.00 25.83 C ATOM 3754 CB TYR 61 109.11852.575 54.861 1.00 12.00 C ATOM 3755 CG TYR 61 109.496 51.237 54.2741.00 18.91 C ATOM 3756 CD1 TYR 61 109.216 50.053 54.953 1.00 42.23 CATOM 3757 CE1 TYR 61 109.599 48.819 54.439 1.00 33.05 C ATOM 3758 CD2TYR 61 110.173 51.150 53.057 1.00 38.49 C ATOM 3759 CE2 TYR 61 110.56249.918 52.533 1.00 23.48 C ATOM 3760 CZ TYR 61 110.269 48.758 53.2311.00 29.34 C ATOM 3761 OH TYR 61 110.627 47.535 52.714 1.00 30.14 C ATOM3762 C TYR 61 107.540 52.981 52.986 1.00 35.71 C ATOM 3763 O TYR 61106.694 52.214 52.513 1.00 35.76 C ATOM 3764 N SER 62 108.322 53.77552.258 1.00 39.08 C ATOM 3765 CA SER 62 108.268 53.813 50.805 1.00 39.42C ATOM 3766 CB SER 62 107.247 54.857 50.345 1.00 32.31 C ATOM 3767 OGSER 62 107.120 54.853 48.932 1.00 23.48 C ATOM 3768 C SER 62 109.64354.137 50.220 1.00 45.43 C ATOM 3769 O SER 62 110.287 55.116 50.601 1.0042.20 C ATOM 3770 N LYS 63 110.094 53.302 49.294 1.00 50.45 C ATOM 3771CA LYS 63 111.388 53.499 48.655 1.00 44.30 C ATOM 3772 CB LYS 63 112.04052.136 48.373 1.00 41.16 C ATOM 3773 CG LYS 63 113.045 51.676 49.4311.00 41.13 C ATOM 3774 CD LYS 63 112.720 50.288 49.969 1.00 36.52 C ATOM3775 CE LYS 63 113.769 49.809 50.972 1.00 27.51 C ATOM 3776 NZ LYS 63114.917 49.099 50.322 1.00 14.01 C ATOM 3777 C LYS 63 111.173 54.24347.343 1.00 40.39 C ATOM 3778 O LYS 63 112.079 54.322 46.515 1.00 43.49C ATOM 3779 N THR 64 109.977 54.802 47.166 1.00 38.77 C ATOM 3780 CA THR64 109.635 55.496 45.926 1.00 33.97 C ATOM 3781 CB THR 64 108.407 54.83745.264 1.00 45.65 C ATOM 3782 OG1 THR 64 107.445 54.502 46.272 1.0061.07 C ATOM 3783 CG2 THR 64 108.811 53.576 44.520 1.00 45.91 C ATOM3784 C THR 64 109.360 56.988 46.033 1.00 33.47 C ATOM 3785 O THR 64109.122 57.651 45.023 1.00 25.44 C ATOM 3786 N GLY 65 109.375 57.52547.244 1.00 35.21 C ATOM 3787 CA GLY 65 109.119 58.946 47.383 1.00 35.72C ATOM 3788 C GLY 65 107.644 59.286 47.468 1.00 35.69 C ATOM 3789 O GLY65 107.198 60.317 46.970 1.00 41.32 C ATOM 3790 N PHE 66 106.881 58.40548.100 1.00 31.14 C ATOM 3791 CA PHE 66 105.454 58.616 48.280 1.00 22.44C ATOM 3792 CB PHE 66 104.680 57.350 47.914 1.00 15.68 C ATOM 3793 CGPHE 66 104.307 57.256 46.463 1.00 27.36 C ATOM 3794 CD1 PHE 66 105.24356.859 45.514 1.00 38.42 C ATOM 3795 CD2 PHE 66 103.009 57.536 46.0421.00 42.24 C ATOM 3796 CE1 PHE 66 104.889 56.738 44.159 1.00 42.03 CATOM 3797 CE2 PHE 66 102.648 57.419 44.694 1.00 38.62 C ATOM 3798 CZ PHE66 103.590 57.018 43.750 1.00 28.00 C ATOM 3799 C PHE 66 105.238 58.92149.758 1.00 29.60 C ATOM 3800 O PHE 66 105.701 58.176 50.622 1.00 28.41C ATOM 3801 N ASN 67 104.569 60.028 50.053 1.00 29.26 C ATOM 3802 CA ASN67 104.289 60.375 51.436 1.00 28.71 C ATOM 3803 CB ASN 67 103.776 61.81451.516 1.00 44.50 C ATOM 3804 CG ASN 67 103.832 62.385 52.923 1.00 56.37C ATOM 3805 OD1 ASN 67 103.890 63.603 53.110 1.00 55.38 C ATOM 3806 ND2ASN 67 103.816 61.507 53.922 1.00 49.90 C ATOM 3807 C ASN 67 103.20859.390 51.891 1.00 30.16 C ATOM 3808 O ASN 67 102.018 59.698 51.893 1.0022.60 C ATOM 3809 N CYS 68 103.627 58.190 52.276 1.00 45.93 C ATOM 3810CA CYS 68 102.678 57.165 52.691 1.00 37.84 C ATOM 3811 C CYS 68 102.48056.998 54.198 1.00 40.89 C ATOM 3812 O CYS 68 103.414 57.122 54.986 1.0026.35 C ATOM 3813 CB CYS 68 103.087 55.824 52.089 1.00 23.40 C ATOM 3814SG CYS 68 102.108 54.426 52.713 1.00 53.33 C ATOM 3815 N ASP 69 101.24356.700 54.579 1.00 44.34 C ATOM 3816 CA ASP 69 100.880 56.482 55.9741.00 40.80 C ATOM 3817 CB ASP 69 100.101 57.683 56.515 1.00 56.37 C ATOM3818 CG ASP 69 100.821 59.001 56.287 1.00 59.33 C ATOM 3819 OD1 ASP 69100.303 59.840 55.520 1.00 66.37 C ATOM 3820 OD2 ASP 69 101.902 59.20056.877 1.00 68.80 C ATOM 3821 C ASP 69 100.001 55.240 56.013 1.00 32.24C ATOM 3822 O ASP 69 99.172 55.051 55.128 1.00 33.63 C ATOM 3823 N GLY70 100.175 54.395 57.026 1.00 34.87 C ATOM 3824 CA GLY 70 99.369 53.18457.107 1.00 28.23 C ATOM 3825 C GLY 70 98.691 52.933 58.444 1.00 28.75 CATOM 3826 O GLY 70 99.260 53.198 59.501 1.00 34.76 C ATOM 3827 N LYS 7197.468 52.416 58.394 1.00 40.41 C ATOM 3828 CA LYS 71 96.697 52.12359.599 1.00 49.78 C ATOM 3829 CB LYS 71 95.522 53.095 59.714 1.00 57.09C ATOM 3830 CG LYS 71 95.747 54.285 60.629 1.00 56.31 C ATOM 3831 CD LYS71 94.477 55.139 60.720 1.00 55.32 C ATOM 3832 CE LYS 71 94.459 56.00461.984 1.00 59.27 C ATOM 3833 NZ LYS 71 93.094 56.536 62.310 1.00 33.49C ATOM 3834 C LYS 71 96.160 50.690 59.553 1.00 51.01 C ATOM 3835 O LYS71 95.553 50.274 58.568 1.00 42.24 C ATOM 3836 N LEU 72 96.373 49.94060.628 1.00 51.79 C ATOM 3837 CA LEU 72 95.907 48.564 60.684 1.00 61.74C ATOM 3838 CB LEU 72 96.773 47.760 61.650 1.00 62.34 C ATOM 3839 CG LEU72 97.284 46.425 61.103 1.00 66.36 C ATOM 3840 CD1 LEU 72 98.165 45.75462.147 1.00 68.74 C ATOM 3841 CD2 LEU 72 96.107 45.527 60.736 1.00 53.65C ATOM 3842 C LEU 72 94.436 48.447 61.090 1.00 73.73 C ATOM 3843 O LEU72 93.860 49.374 61.663 1.00 88.59 C ATOM 3844 N GLY 73 93.835 47.29860.789 1.00 67.09 C ATOM 3845 CA GLY 73 92.443 47.075 61.126 1.00 50.60C ATOM 3846 C GLY 73 91.964 45.670 60.808 1.00 56.73 C ATOM 3847 O GLY73 91.356 45.434 59.760 1.00 64.99 C ATOM 3848 N ASN 74 92.246 44.73561.713 1.00 46.04 C ATOM 3849 CA ASN 74 91.828 43.342 61.566 1.00 54.16C ATOM 3850 CB ASN 74 90.316 43.231 61.790 1.00 79.65 C ATOM 3851 CG ASN74 89.953 42.174 62.814 1.00 90.87 C ATOM 3852 OD1 ASN 74 88.992 42.33463.574 1.00 90.68 C ATOM 3853 ND2 ASN 74 90.715 41.086 62.838 1.00 89.35C ATOM 3854 C ASN 74 92.180 42.738 60.215 1.00 54.40 C ATOM 3855 O ASN74 93.332 42.413 59.951 1.00 62.07 C ATOM 3856 N GLU 75 91.171 42.57759.364 1.00 55.88 C ATOM 3857 CA GLU 75 91.364 42.010 58.035 1.00 47.09C ATOM 3858 CB GLU 75 90.269 40.988 57.722 1.00 56.56 C ATOM 3859 CG GLU75 88.966 41.211 58.471 1.00 48.68 C ATOM 3860 CD GLU 75 88.614 40.04759.379 1.00 58.55 C ATOM 3861 OE1 GLU 75 88.407 38.928 58.859 1.00 55.16C ATOM 3862 OE2 GLU 75 88.547 40.250 60.612 1.00 54.08 C ATOM 3863 C GLU75 91.323 43.127 57.013 1.00 44.01 C ATOM 3864 O GLU 75 90.606 43.05556.017 1.00 27.15 C ATOM 3865 N SER 76 92.107 44.162 57.268 1.00 36.46 CATOM 3866 CA SER 76 92.143 45.296 56.376 1.00 31.85 C ATOM 3867 CB SER76 90.835 46.072 56.492 1.00 24.92 C ATOM 3868 OG SER 76 90.835 46.83557.681 1.00 16.45 C ATOM 3869 C SER 76 93.292 46.208 56.733 1.00 32.47 CATOM 3870 O SER 76 93.689 46.294 57.892 1.00 34.00 C ATOM 3871 N VAL 7793.826 46.884 55.723 1.00 38.60 C ATOM 3872 CA VAL 77 94.906 47.84855.908 1.00 32.38 C ATOM 3873 CB VAL 77 96.272 47.308 55.386 1.00 45.71C ATOM 3874 CG1 VAL 77 96.747 48.122 54.190 1.00 37.47 C ATOM 3875 CG2VAL 77 97.327 47.372 56.484 1.00 19.29 C ATOM 3876 C VAL 77 94.50949.083 55.101 1.00 33.67 C ATOM 3877 O VAL 77 93.934 48.972 54.013 1.0038.06 C ATOM 3878 N THR 78 94.811 50.260 55.630 1.00 40.03 C ATOM 3879CA THR 78 94.475 51.493 54.929 1.00 38.24 C ATOM 3880 CB THR 78 93.54852.377 55.787 1.00 35.10 C ATOM 3881 OG1 THR 78 92.594 51.551 56.4721.00 36.53 C ATOM 3882 CG2 THR 78 92.804 53.363 54.916 1.00 36.45 C ATOM3883 C THR 78 95.745 52.269 54.580 1.00 35.91 C ATOM 3884 O THR 7896.574 52.540 55.451 1.00 31.92 C ATOM 3885 N PHE 79 95.896 52.61053.301 1.00 39.13 C ATOM 3886 CA PHE 79 97.059 53.358 52.824 1.00 29.89C ATOM 3887 CB PHE 79 97.532 52.801 51.487 1.00 35.47 C ATOM 3888 CG PHE79 97.996 51.380 51.553 1.00 41.67 C ATOM 3889 CD1 PHE 79 97.174 50.34251.123 1.00 46.33 C ATOM 3890 CD2 PHE 79 99.271 51.077 52.019 1.00 35.39C ATOM 3891 CE1 PHE 79 97.621 49.026 51.155 1.00 34.72 C ATOM 3892 CE2PHE 79 99.724 49.765 52.054 1.00 17.78 C ATOM 3893 CZ PHE 79 98.89548.736 51.619 1.00 26.82 C ATOM 3894 C PHE 79 96.687 54.816 52.645 1.0020.01 C ATOM 3895 O PHE 79 95.843 55.143 51.817 1.00 39.07 C ATOM 3896 NTYR 80 97.328 55.690 53.414 1.00 37.92 C ATOM 3897 CA TYR 80 97.04857.123 53.361 1.00 39.66 C ATOM 3898 CB TYR 80 97.043 57.706 54.775 1.0041.42 C ATOM 3899 CG TYR 80 96.541 59.125 54.849 1.00 29.87 C ATOM 3900CD1 TYR 80 95.857 59.702 53.782 1.00 31.82 C ATOM 3901 CE1 TYR 80 95.40461.008 53.842 1.00 38.26 C ATOM 3902 CD2 TYR 80 96.756 59.895 55.9841.00 35.48 C ATOM 3903 CE2 TYR 80 96.307 61.207 56.060 1.00 38.78 C ATOM3904 CZ TYR 80 95.632 61.758 54.984 1.00 46.52 C ATOM 3905 OH TYR 8095.195 63.066 55.046 1.00 43.37 C ATOM 3906 C TYR 80 98.034 57.90152.508 1.00 36.43 C ATOM 3907 O TYR 80 98.960 58.529 53.028 1.00 41.51 CATOM 3908 N LEU 81 97.817 57.873 51.199 1.00 37.66 C ATOM 3909 CA LEU 8198.694 58.576 50.268 1.00 43.35 C ATOM 3910 CB LEU 81 98.538 57.99748.861 1.00 40.97 C ATOM 3911 CG LEU 81 98.392 56.485 48.721 1.00 35.20C ATOM 3912 CD1 LEU 81 98.068 56.154 47.268 1.00 41.59 C ATOM 3913 CD2LEU 81 99.670 55.798 49.141 1.00 34.03 C ATOM 3914 C LEU 81 98.45760.092 50.216 1.00 39.41 C ATOM 3915 O LEU 81 97.410 60.552 49.759 1.0030.90 C ATOM 3916 N GLN 82 99.450 60.853 50.678 1.00 45.56 C ATOM 3917CA GLN 82 99.397 62.315 50.681 1.00 47.39 C ATOM 3918 CB GLN 82 99.88462.868 52.023 1.00 48.95 C ATOM 3919 CG GLN 82 99.479 62.062 53.246 1.0042.53 C ATOM 3920 CD GLN 82 99.397 62.921 54.491 1.00 35.76 C ATOM 3921OE1 GLN 82 99.826 62.514 55.575 1.00 35.27 C ATOM 3922 NE2 GLN 82 98.85764.127 54.339 1.00 29.92 C ATOM 3923 C GLN 82 100.280 62.884 49.571 1.0042.88 C ATOM 3924 O GLN 82 101.148 62.194 49.035 1.00 41.58 C ATOM 3925N ASN 83 100.060 64.148 49.237 1.00 45.46 C ATOM 3926 CA ASN 83 100.84364.806 48.197 1.00 43.88 C ATOM 3927 CB ASN 83 102.229 65.139 48.7481.00 38.56 C ATOM 3928 CG ASN 83 102.170 66.242 49.779 1.00 47.82 C ATOM3929 OD1 ASN 83 101.274 67.083 49.735 1.00 46.58 C ATOM 3930 ND2 ASN 83103.118 66.249 50.715 1.00 33.98 C ATOM 3931 C ASN 83 100.951 63.96646.933 1.00 37.91 C ATOM 3932 O ASN 83 102.045 63.718 46.424 1.00 45.24C ATOM 3933 N LEU 84 99.803 63.535 46.430 1.00 28.03 C ATOM 3934 CA LEU84 99.760 62.723 45.230 1.00 19.39 C ATOM 3935 CB LEU 84 98.540 61.80245.263 1.00 26.73 C ATOM 3936 CG LEU 84 98.774 60.341 45.664 1.00 34.93C ATOM 3937 CD1 LEU 84 97.483 59.552 45.502 1.00 37.57 C ATOM 3938 CD2LEU 84 99.877 59.729 44.812 1.00 36.66 C ATOM 3939 C LEU 84 99.67563.650 44.037 1.00 20.07 C ATOM 3940 O LEU 84 98.914 64.609 44.049 1.0031.39 C ATOM 3941 N TYR 85 100.460 63.365 43.006 1.00 33.90 C ATOM 3942CA TYR 85 100.464 64.187 41.806 1.00 40.42 C ATOM 3943 CB TYR 85 101.84064.117 41.123 1.00 59.23 C ATOM 3944 CG TYR 85 102.989 64.613 41.9801.00 61.79 C ATOM 3945 CD1 TYR 85 103.155 65.973 42.242 1.00 63.76 CATOM 3946 CE1 TYR 85 104.193 66.430 43.053 1.00 67.56 C ATOM 3947 CD2TYR 85 103.897 63.717 42.549 1.00 64.22 C ATOM 3948 CE2 TYR 85 104.93864.162 43.360 1.00 65.83 C ATOM 3949 CZ TYR 85 105.079 65.519 43.6101.00 70.24 C ATOM 3950 OH TYR 85 106.093 65.965 44.428 1.00 73.68 C ATOM3951 C TYR 85 99.378 63.724 40.841 1.00 33.25 C ATOM 3952 O TYR 8598.880 62.606 40.943 1.00 33.16 C ATOM 3953 N VAL 86 99.018 64.59839.907 1.00 32.80 C ATOM 3954 CA VAL 86 97.994 64.297 38.914 1.00 31.92C ATOM 3955 CB VAL 86 97.503 65.600 38.241 1.00 29.33 C ATOM 3956 CG1VAL 86 98.476 66.730 38.548 1.00 32.20 C ATOM 3957 CG2 VAL 86 97.36165.409 36.743 1.00 34.84 C ATOM 3958 C VAL 86 98.504 63.312 37.854 1.0030.06 C ATOM 3959 O VAL 86 97.721 62.621 37.204 1.00 28.12 C ATOM 3960 NASN 87 99.819 63.246 37.685 1.00 37.39 C ATOM 3961 CA ASN 87 100.41162.330 36.716 1.00 31.78 C ATOM 3962 CB ASN 87 101.803 62.811 36.3481.00 30.37 C ATOM 3963 CG ASN 87 102.721 62.866 37.542 1.00 15.74 C ATOM3964 OD1 ASN 87 102.334 62.461 38.631 1.00 26.61 C ATOM 3965 ND2 ASN 87103.939 63.370 37.350 1.00 44.07 C ATOM 3966 C ASN 87 100.504 60.95537.374 1.00 31.73 C ATOM 3967 O ASN 87 100.964 59.981 36.773 1.00 29.36C ATOM 3968 N GLN 88 100.063 60.897 38.627 1.00 33.61 C ATOM 3969 CA GLN88 100.082 59.665 39.402 1.00 24.73 C ATOM 3970 CB GLN 88 100.430 59.98340.871 1.00 26.06 C ATOM 3971 CG GLN 88 101.721 59.314 41.400 1.00 44.43C ATOM 3972 CD GLN 88 102.692 60.285 42.081 1.00 36.54 C ATOM 3973 OE1GLN 88 102.504 60.675 43.235 1.00 26.99 C ATOM 3974 NE2 GLN 88 103.74360.668 41.363 1.00 38.97 C ATOM 3975 C GLN 88 98.747 58.896 39.322 1.0026.16 C ATOM 3976 O GLN 88 98.635 57.805 39.872 1.00 22.71 C ATOM 3977 NTHR 89 97.735 59.448 38.645 1.00 29.74 C ATOM 3978 CA THR 89 96.45058.742 38.548 1.00 35.51 C ATOM 3979 CB THR 89 95.381 59.548 37.786 1.0023.56 C ATOM 3980 OG1 THR 89 95.586 59.391 36.381 1.00 37.82 C ATOM 3981CG2 THR 89 95.456 61.008 38.140 1.00 8.56 C ATOM 3982 C THR 89 96.68657.434 37.808 1.00 30.57 C ATOM 3983 O THR 89 97.222 57.427 36.701 1.0019.99 C ATOM 3984 N ASP 90 96.274 56.326 38.411 1.00 26.02 C ATOM 3985CA ASP 90 96.520 55.032 37.799 1.00 29.88 C ATOM 3986 CB ASP 90 98.02354.758 37.848 1.00 17.27 C ATOM 3987 CG ASP 90 98.492 53.857 36.738 1.0029.31 C ATOM 3988 OD1 ASP 90 99.661 54.010 36.312 1.00 28.02 C ATOM 3989OD2 ASP 90 97.695 53.002 36.299 1.00 35.12 C ATOM 3990 C ASP 90 95.77453.918 38.519 1.00 32.73 C ATOM 3991 O ASP 90 94.869 54.181 39.307 1.0028.33 C ATOM 3992 N ILE 91 96.155 52.674 38.234 1.00 33.15 C ATOM 3993CA ILE 91 95.549 51.524 38.891 1.00 29.34 C ATOM 3994 CB ILE 91 95.32050.330 37.960 1.00 19.19 C ATOM 3995 CG2 ILE 91 93.891 49.803 38.1241.00 7.46 C ATOM 3996 CG1 ILE 91 95.613 50.734 36.525 1.00 24.90 C ATOM3997 CD1 ILE 91 96.736 49.967 35.904 1.00 37.36 C ATOM 3998 C ILE 9196.539 51.070 39.929 1.00 33.79 C ATOM 3999 O ILE 91 97.707 50.84439.615 1.00 35.59 C ATOM 4000 N TYR 92 96.064 50.948 41.162 1.00 39.40 CATOM 4001 CA TYR 92 96.891 50.521 42.274 1.00 25.36 C ATOM 4002 CB TYR92 96.817 51.555 43.398 1.00 24.66 C ATOM 4003 CG TYR 92 97.472 52.87243.039 1.00 27.16 C ATOM 4004 CD1 TYR 92 98.646 53.282 43.669 1.00 32.14C ATOM 4005 CE1 TYR 92 99.265 54.474 43.327 1.00 24.99 C ATOM 4006 CD2TYR 92 96.933 53.697 42.051 1.00 24.44 C ATOM 4007 CE2 TYR 92 97.54754.896 41.703 1.00 24.44 C ATOM 4008 CZ TYR 92 98.712 55.275 42.344 1.0018.97 C ATOM 4009 OH TYR 92 99.332 56.454 42.010 1.00 18.71 C ATOM 4010C TYR 92 96.381 49.179 42.761 1.00 25.56 C ATOM 4011 O TYR 92 95.17348.942 42.791 1.00 36.33 C ATOM 4012 N PHE 93 97.303 48.298 43.127 1.0028.40 C ATOM 4013 CA PHE 93 96.931 46.984 43.621 1.00 29.94 C ATOM 4014CB PHE 93 97.448 45.893 42.689 1.00 17.97 C ATOM 4015 CG PHE 93 97.10546.113 41.258 1.00 24.43 C ATOM 4016 CD1 PHE 93 97.978 46.793 40.4221.00 27.44 C ATOM 4017 CD2 PHE 93 95.921 45.617 40.733 1.00 30.96 C ATOM4018 CE1 PHE 93 97.673 46.973 39.076 1.00 46.97 C ATOM 4019 CE2 PHE 9395.610 45.792 39.391 1.00 31.89 C ATOM 4020 CZ PHE 93 96.483 46.46738.562 1.00 33.13 C ATOM 4021 C PHE 93 97.470 46.739 45.020 1.00 35.26 CATOM 4022 O PHE 93 98.635 47.022 45.325 1.00 34.73 C ATOM 4023 N CYS 9496.601 46.209 45.870 1.00 48.06 C ATOM 4024 CA CYS 94 96.969 45.90747.235 1.00 36.83 C ATOM 4025 C CYS 94 97.635 44.546 47.284 1.00 31.31 CATOM 4026 O CYS 94 97.397 43.692 46.431 1.00 33.02 C ATOM 4027 CB CYS 9495.738 45.921 48.121 1.00 39.16 C ATOM 4028 SG CYS 94 96.102 46.71149.706 1.00 59.22 C ATOM 4029 N LYS 95 98.475 44.349 48.288 1.00 35.15 CATOM 4030 CA LYS 95 99.194 43.093 48.430 1.00 32.02 C ATOM 4031 CB LYS95 100.593 43.227 47.824 1.00 20.95 C ATOM 4032 CG LYS 95 101.265 41.91347.532 1.00 16.51 C ATOM 4033 CD LYS 95 102.720 42.111 47.179 1.00 20.20C ATOM 4034 CE LYS 95 103.419 40.772 46.979 1.00 39.20 C ATOM 4035 NZLYS 95 104.812 40.931 46.471 1.00 47.09 C ATOM 4036 C LYS 95 99.30142.731 49.896 1.00 29.30 C ATOM 4037 O LYS 95 99.224 43.602 50.759 1.0032.79 C ATOM 4038 N ILE 96 99.480 41.444 50.172 1.00 24.46 C ATOM 4039CA ILE 96 99.619 40.978 51.542 1.00 19.72 C ATOM 4040 CB ILE 96 98.25840.925 52.251 1.00 14.76 C ATOM 4041 CG2 ILE 96 97.299 40.052 51.4771.00 17.83 C ATOM 4042 CG1 ILE 96 98.434 40.405 53.673 1.00 17.25 C ATOM4043 CD1 ILE 96 99.184 41.352 54.570 1.00 5.02 C ATOM 4044 C ILE 96100.276 39.599 51.602 1.00 26.57 C ATOM 4045 O ILE 96 99.852 38.66250.916 1.00 34.37 C ATOM 4046 N GLU 97 101.321 39.491 52.419 1.00 20.47C ATOM 4047 CA GLU 97 102.052 38.243 52.592 1.00 27.23 C ATOM 4048 CBGLU 97 103.367 38.279 51.793 1.00 32.57 C ATOM 4049 CG GLU 97 104.18639.550 51.949 1.00 38.60 C ATOM 4050 CD GLU 97 104.535 40.193 50.6181.00 46.08 C ATOM 4051 OE1 GLU 97 105.239 39.548 49.815 1.00 37.73 CATOM 4052 OE2 GLU 97 104.108 41.348 50.379 1.00 54.19 C ATOM 4053 C GLU97 102.343 37.951 54.071 1.00 30.22 C ATOM 4054 O GLU 97 102.705 38.84154.844 1.00 27.30 C ATOM 4055 N VAL 98 102.158 36.694 54.455 1.00 24.37C ATOM 4056 CA VAL 98 102.395 36.256 55.817 1.00 17.97 C ATOM 4057 CBVAL 98 101.361 35.183 56.224 1.00 33.66 C ATOM 4058 CG1 VAL 98 102.00234.115 57.113 1.00 46.61 C ATOM 4059 CG2 VAL 98 100.200 35.844 56.9311.00 31.46 C ATOM 4060 C VAL 98 103.787 35.664 55.808 1.00 24.36 C ATOM4061 O VAL 98 104.147 34.979 54.858 1.00 17.44 C ATOM 4062 N MET 99104.569 35.936 56.851 1.00 41.56 C ATOM 4063 CA MET 99 105.932 35.41456.940 1.00 37.25 C ATOM 4064 CB MET 99 106.951 36.540 56.753 1.00 26.44C ATOM 4065 CG MET 99 106.449 37.906 57.176 1.00 37.71 C ATOM 4066 SDMET 99 106.981 39.212 56.039 1.00 49.54 C ATOM 4067 CE MET 99 105.58739.271 54.899 1.00 38.22 C ATOM 4068 C MET 99 106.182 34.728 58.273 1.0037.57 C ATOM 4069 O MET 99 107.286 34.241 58.526 1.00 31.40 C ATOM 4070N TYR 100 105.154 34.712 59.119 1.00 45.50 C ATOM 4071 CA TYR 100105.210 34.084 60.439 1.00 45.47 C ATOM 4072 CB TYR 100 106.441 34.53261.218 1.00 49.87 C ATOM 4073 CG TYR 100 106.979 33.489 62.176 1.0054.53 C ATOM 4074 CD1 TYR 100 107.908 32.545 61.745 1.00 66.10 C ATOM4075 CE1 TYR 100 108.466 31.625 62.622 1.00 67.96 C ATOM 4076 CD2 TYR100 106.611 33.483 63.523 1.00 50.84 C ATOM 4077 CE2 TYR 100 107.16632.559 64.415 1.00 57.94 C ATOM 4078 CZ TYR 100 108.098 31.636 63.9511.00 57.97 C ATOM 4079 OH TYR 100 108.687 30.728 64.801 1.00 53.54 CATOM 4080 C TYR 100 103.978 34.429 61.259 1.00 49.53 C ATOM 4081 O TYR100 103.561 35.587 61.307 1.00 42.87 C ATOM 4082 N PRO 101 103.38033.422 61.920 1.00 53.02 C ATOM 4083 CD PRO 101 102.180 33.528 62.7711.00 52.41 C ATOM 4084 CA PRO 101 103.902 32.054 61.844 1.00 46.66 CATOM 4085 CB PRO 101 103.126 31.300 62.927 1.00 42.16 C ATOM 4086 CG PRO101 101.906 32.115 63.189 1.00 38.19 C ATOM 4087 C PRO 101 103.71131.468 60.441 1.00 57.32 C ATOM 4088 O PRO 101 102.901 31.960 59.6481.00 47.74 C ATOM 4089 N PRO 102 104.455 30.400 60.122 1.00 58.81 C ATOM4090 CD PRO 102 105.410 29.707 61.000 1.00 43.99 C ATOM 4091 CA PRO 102104.368 29.758 58.807 1.00 54.29 C ATOM 4092 CB PRO 102 105.585 28.82458.766 1.00 46.96 C ATOM 4093 CG PRO 102 106.354 29.090 60.030 1.0046.28 C ATOM 4094 C PRO 102 103.072 28.997 58.565 1.00 50.83 C ATOM 4095O PRO 102 102.265 28.812 59.475 1.00 51.79 C ATOM 4096 N PRO 103 102.85828.550 57.321 1.00 43.58 C ATOM 4097 CD PRO 103 101.692 27.757 56.9011.00 38.84 C ATOM 4098 CA PRO 103 103.784 28.756 56.209 1.00 34.80 CATOM 4099 CB PRO 103 103.457 27.611 55.247 1.00 35.84 C ATOM 4100 CG PRO103 102.280 26.863 55.860 1.00 31.11 C ATOM 4101 C PRO 103 103.58130.127 55.566 1.00 39.20 C ATOM 4102 O PRO 103 102.790 30.939 56.0551.00 35.30 C ATOM 4103 N TYR 104 104.311 30.382 54.481 1.00 40.51 C ATOM4104 CA TYR 104 104.199 31.644 53.760 1.00 40.50 C ATOM 4105 CB TYR 104105.226 31.719 52.630 1.00 34.12 C ATOM 4106 CG TYR 104 105.224 33.03651.888 1.00 35.99 C ATOM 4107 CD1 TYR 104 104.659 33.143 50.615 1.0049.18 C ATOM 4108 CE1 TYR 104 104.652 34.363 49.926 1.00 50.34 C ATOM4109 CD2 TYR 104 105.784 34.179 52.458 1.00 34.98 C ATOM 4110 CE2 TYR104 105.782 35.403 51.779 1.00 35.62 C ATOM 4111 CZ TYR 104 105.21535.485 50.518 1.00 39.99 C ATOM 4112 OH TYR 104 105.205 36.685 49.8531.00 32.85 C ATOM 4113 C TYR 104 102.804 31.725 53.168 1.00 44.36 C ATOM4114 O TYR 104 102.311 30.749 52.603 1.00 46.76 C ATOM 4115 N LEU 105102.171 32.885 53.304 1.00 47.99 C ATOM 4116 CA LEU 105 100.827 33.09652.778 1.00 43.06 C ATOM 4117 CB LEU 105 99.859 33.347 53.929 1.00 44.75C ATOM 4118 CG LEU 105 98.748 32.310 54.097 1.00 55.87 C ATOM 4119 CD1LEU 105 98.181 31.958 52.725 1.00 60.46 C ATOM 4120 CD2 LEU 105 99.28931.062 54.797 1.00 56.52 C ATOM 4121 C LEU 105 100.819 34.283 51.8131.00 45.31 C ATOM 4122 O LEU 105 101.438 35.308 52.085 1.00 44.10 C ATOM4123 N ASP 106 100.107 34.144 50.696 1.00 53.21 C ATOM 4124 CA ASP 106100.046 35.199 49.681 1.00 56.65 C ATOM 4125 CB ASP 106 100.961 34.82748.512 1.00 73.34 C ATOM 4126 CG ASP 106 101.724 36.011 47.979 1.0081.75 C ATOM 4127 OD1 ASP 106 101.437 37.143 48.432 1.00 85.62 C ATOM4128 OD2 ASP 106 102.603 35.802 47.109 1.00 80.61 C ATOM 4129 C ASP 10698.639 35.462 49.145 1.00 52.61 C ATOM 4130 O ASP 106 97.772 34.59249.199 1.00 62.59 C ATOM 4131 N ASN 107 98.422 36.658 48.609 1.00 42.29C ATOM 4132 CA ASN 107 97.110 37.028 48.072 1.00 50.80 C ATOM 4133 CBASN 107 96.826 38.492 48.359 1.00 42.50 C ATOM 4134 CG ASN 107 97.85539.393 47.736 1.00 41.45 C ATOM 4135 OD1 ASN 107 97.691 39.852 46.6071.00 44.69 C ATOM 4136 ND2 ASN 107 98.940 39.637 48.461 1.00 38.52 CATOM 4137 C ASN 107 96.997 36.815 46.564 1.00 55.83 C ATOM 4138 O ASN107 97.990 36.865 45.840 1.00 54.74 C ATOM 4139 N GLU 108 95.771 36.60146.098 1.00 61.82 C ATOM 4140 CA GLU 108 95.513 36.393 44.678 1.00 62.32C ATOM 4141 CB GLU 108 94.305 35.474 44.485 1.00 65.84 C ATOM 4142 CGGLU 108 93.817 34.809 45.757 1.00 72.69 C ATOM 4143 CD GLU 108 92.44535.295 46.180 1.00 73.87 C ATOM 4144 OE1 GLU 108 91.534 34.453 46.3271.00 58.56 C ATOM 4145 OE2 GLU 108 92.285 36.522 46.366 1.00 79.05 CATOM 4146 C GLU 108 95.244 37.722 43.991 1.00 64.40 C ATOM 4147 O GLU108 94.094 38.171 43.936 1.00 61.70 C ATOM 4148 N LYS 109 96.306 38.33943.467 1.00 64.63 C ATOM 4149 CA LYS 109 96.215 39.629 42.782 1.00 60.84C ATOM 4150 CB LYS 109 96.054 39.420 41.274 1.00 43.96 C ATOM 4151 CGLYS 109 97.094 38.496 40.666 1.00 66.29 C ATOM 4152 CD LYS 109 98.39939.235 40.373 1.00 84.02 C ATOM 4153 CE LYS 109 99.309 38.435 39.4321.00 77.45 C ATOM 4154 NZ LYS 109 100.234 39.299 38.636 1.00 53.39 CATOM 4155 C LYS 109 95.040 40.435 43.331 1.00 59.47 C ATOM 4156 O LYS109 94.893 40.571 44.545 1.00 73.09 C ATOM 4157 N SER 110 94.201 40.95042.440 1.00 49.53 C ATOM 4158 CA SER 110 93.038 41.734 42.841 1.00 50.60C ATOM 4159 CB SER 110 93.404 42.706 43.969 1.00 48.38 C ATOM 4160 OGSER 110 93.807 43.962 43.448 1.00 55.94 C ATOM 4161 C SER 110 92.45542.520 41.670 1.00 49.16 C ATOM 4162 O SER 110 93.098 42.693 40.634 1.0036.85 C ATOM 4163 N ASN 111 91.228 43.000 41.841 1.00 50.24 C ATOM 4164CA ASN 111 90.580 43.770 40.795 1.00 48.26 C ATOM 4165 CB ASN 111 89.07343.868 41.068 1.00 50.05 C ATOM 4166 CG ASN 111 88.273 42.770 40.3641.00 52.55 C ATOM 4167 OD1 ASN 111 87.101 42.539 40.671 1.00 30.90 CATOM 4168 ND2 ASN 111 88.912 42.089 39.413 1.00 60.36 C ATOM 4169 C ASN111 91.212 45.162 40.708 1.00 42.47 C ATOM 4170 O ASN 111 90.982 45.89639.748 1.00 48.01 C ATOM 4171 N GLY 112 92.007 45.517 41.717 1.00 44.01C ATOM 4172 CA GLY 112 92.682 46.808 41.729 1.00 36.00 C ATOM 4173 C GLY112 91.830 48.015 42.081 1.00 36.58 C ATOM 4174 O GLY 112 90.630 47.89242.343 1.00 33.31 C ATOM 4175 N THR 113 92.455 49.189 42.094 1.00 28.16C ATOM 4176 CA THR 113 91.738 50.416 42.403 1.00 29.29 C ATOM 4177 CBTHR 113 91.671 50.667 43.929 1.00 25.84 C ATOM 4178 OG1 THR 113 90.95951.884 44.186 1.00 13.15 C ATOM 4179 CG2 THR 113 93.061 50.762 44.5141.00 35.81 C ATOM 4180 C THR 113 92.344 51.636 41.722 1.00 29.94 C ATOM4181 O THR 113 93.375 52.157 42.133 1.00 21.21 C ATOM 4182 N ILE 11491.679 52.074 40.662 1.00 31.79 C ATOM 4183 CA ILE 114 92.093 53.24339.905 1.00 24.64 C ATOM 4184 CB ILE 114 91.132 53.521 38.721 1.00 29.78C ATOM 4185 CG2 ILE 114 91.159 55.001 38.361 1.00 29.89 C ATOM 4186 CG1ILE 114 91.510 52.661 37.519 1.00 45.18 C ATOM 4187 CD1 ILE 114 91.37653.376 36.182 1.00 48.49 C ATOM 4188 C ILE 114 92.019 54.450 40.821 1.0021.71 C ATOM 4189 O ILE 114 91.050 54.632 41.546 1.00 23.04 C ATOM 4190N ILE 115 93.028 55.296 40.770 1.00 22.63 C ATOM 4191 CA ILE 115 93.00356.483 41.593 1.00 25.37 C ATOM 4192 CB ILE 115 94.147 56.461 42.5851.00 19.98 C ATOM 4193 CG2 ILE 115 94.249 57.798 43.307 1.00 15.47 CATOM 4194 CG1 ILE 115 93.925 55.312 43.558 1.00 5.93 C ATOM 4195 CD1 ILE115 95.089 55.086 44.508 1.00 25.93 C ATOM 4196 C ILE 115 93.104 57.71240.701 1.00 36.88 C ATOM 4197 O ILE 115 94.147 57.984 40.112 1.00 39.31C ATOM 4198 N HIS 116 92.003 58.445 40.604 1.00 36.64 C ATOM 4199 CA HIS116 91.951 59.641 39.779 1.00 42.31 C ATOM 4200 CB HIS 116 90.530 59.85439.250 1.00 55.50 C ATOM 4201 CG HIS 116 90.397 61.008 38.303 1.00 53.01C ATOM 4202 CD2 HIS 116 89.311 61.534 37.690 1.00 53.38 C ATOM 4203 ND1HIS 116 91.473 61.764 37.884 1.00 49.21 C ATOM 4204 CE1 HIS 116 91.05462.700 37.058 1.00 52.55 C ATOM 4205 NE2 HIS 116 89.744 62.584 36.9201.00 46.03 C ATOM 4206 C HIS 116 92.380 60.847 40.592 1.00 38.13 C ATOM4207 O HIS 116 91.655 61.278 41.486 1.00 35.69 C ATOM 4208 N VAL 11793.554 61.387 40.274 1.00 35.30 C ATOM 4209 CA VAL 117 94.078 62.55040.980 1.00 36.81 C ATOM 4210 CB VAL 117 95.606 62.463 41.146 1.00 24.40C ATOM 4211 CG1 VAL 117 96.075 63.529 42.109 1.00 30.24 C ATOM 4212 CG2VAL 117 96.004 61.087 41.647 1.00 21.51 C ATOM 4213 C VAL 117 93.74763.857 40.271 1.00 44.32 C ATOM 4214 O VAL 117 93.901 63.973 39.054 1.0048.83 C ATOM 4215 N LYS 118 93.304 64.837 41.055 1.00 61.70 C ATOM 4216CA LYS 118 92.926 66.160 40.555 1.00 65.79 C ATOM 4217 CB LYS 118 94.07666.809 39.779 1.00 48.95 C ATOM 4218 CG LYS 118 94.137 68.329 39.9341.00 51.90 C ATOM 4219 CD LYS 118 93.956 69.065 38.603 1.00 54.76 C ATOM4220 CE LYS 118 94.163 70.575 38.754 1.00 49.49 C ATOM 4221 NZ LYS 11895.564 71.003 38.435 1.00 45.55 C ATOM 4222 C LYS 118 91.693 66.10739.664 1.00 78.13 C ATOM 4223 O LYS 118 91.566 67.006 38.807 1.00 88.53C ATOM 4224 OT LYS 118 90.872 65.178 39.832 1.00 83.59 C END

TABLE 5 DNA sequence of human CD28 cDNA (SEQ ID NO: 15)agactctcag gccttggcag gtgcgtcttt cagttcccct cacacttcgg gttcctcggg   60gaggaggggc tggaacccta gcccatcgtc aggacaaaga tgctcaggct gctcttggct  120ctcaacttat tcccttcaat tcaagtaaca ggaaacaaga ttttggtgaa gcagtcgccc  180atgcttgtag cgtacgacaa tgcggtcaac cttagctgca agtattccta caatctcttc  240tcaagggagt tccgggcatc ccttcacaaa ggactggata gtgctgtgga agtctgtgtt  300gtatatggga attactccca gcagcttcag gtttactcaa aaacggggtt caactgtgat  360gggaaattgg gcaatgaatc agtgacattc tacctccaga atttgtatgt taaccaaaca  420gatatttact tctgcaaaat tgaagttatg tatcctcctc cttacctaga caatgagaag  480agcaatggaa ccattatcca tgtgaaaggg aaacaccttt gtccaagtcc cctatttccc  540ggaccttcta agcccttttg ggtgctggtg gtggttggtg gagtcctggc ttgctatagc  600ttgctagtaa cagtggcctt tattattttc tgggtgagga gtaagaggag caggctcctg  660cacagtgact acatgaacat gactccccgc cgccccgggc ccacccgcaa gcattaccag  720ccctatgccc caccacgcga cttcgcagcc tatcgctcct gacacggacg cctatccaga  780agccagccgg ctggcagccc ccatctgctc aatatcactg ctctggatag gaaatgaccg  840ccatctccag ccggccacct cagcccctgt tgggccacca atgccaattt ttctcgagtg  900actagaccaa atatcaagat cattttgaga ctctgaaatg aagtaaaaga gatttcctgt  960gacaggccaa gtcttacagt gccatggccc acattccaac ttaccatgta cttagtgact 1020tgactgagaa gttagggtag aaaacaaaaa gggagtggat tctgggagcc tcttcccttt 1080ctcactcacc tgcacatctc agtcaagcaa agtgtggtat ccacagacat tttagttgca 1140gaagaaaggc taggaaatca ttccttttgg ttaaatgggt gtttaatctt ttggttagtg 1200ggttaaacgg ggtaagttag agtaggggga gggataggaa gacatattta aaaaccatta 1260aaacactgtc tcccactcat gaaatgagcc acgtagttcc tatttaatgc tgttttcctt 1320tagtttagaa atacatagac attgtctttt atgaattctg atcatattta gtcattttga 1380ccaaatgagg gatttggtca aatgagggat tocctcaaag caatatcagg taaaccaagt 1440tgctttcctc accccctgtc atgagacttc agcgttaatg ttcacaatat actttcgaaa 1500gaataaaata gttc 1514 Amino acid sequence of human CD28 (SEQ ID NO: 1)MLRLLLALNL FPSIQVTGNK ILVKQSPMLV AYDNAVNLSC KYSYNLFSRE FRASLHKGLDSAVEVCVVYG NYSQQLQVYS KTGFNCDGKL GNESVTFYLQ NLYVNQTDIY FCKIEVMYPPPYLDNEKSNG TIIHV KGKHL CPSPLFPGPS KPFWVLVVVG GVLACYSLLV TVAFIIFWVRSKRSRLLHSD YMNMTPRRPG PTRKHYQPYA PPRDFAAYRS The extracellular domain isshown in bold The stalk region is underlined

TABLE 6 CD28TFc sequence (SEQ ID NO: 2)(thrombin site separating the two halves of the chimera is shown in bold)CCCCATCCGCTCAAGCAGGCCACCATGGATTGGCTGCGGAACTTGCTATTCCTGATGGCG    1−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+   60GGGGTAGGCGAGTTCGTCCGGTGGTACCTAACCGACGCCTTGAACGATAAGGACTACCGC a                        M  D  W  L  R  N  L  L  F  L  M  A —GCCGCTCAAAGTATCAACGCGAACAAGATCTTGGTGAAGCAGTCGCCCATGCTTGTAGCG   61−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+  120CGGCGAGTTTCATAGTTGCGCTTGTTCTAGAACCACTTCGTCAGCGGGTACGAACATCGC aA  A  Q  S  I  N  A  N  K  I  L  V  K  Q  S  P  M  L  V  A —TACGACAATGCGCTCAACCTTAGCTGCAAGTATTCCTACAATCTCTTCTCAAGGGAGTTC  121−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+  180ATGCTGTTACGCCAGTTGGAATCGACGTTCATAAGGATGTTAGAGAAGAGTTCCCTCAAG aY  D  N  A  V  N  L  S  C  K  Y  S  Y  N  L  F  S  R  E  F —CGGGCATCCCTTCACAAAGGACTGCATAGTGCTGTGGAAGTCTGTGTTGTATATGGGAAT  181−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+  240GCCCGTAGGGAAGTGTTTCCTGACCTATCACGACACCTTCAGACACAACATATACCCTTA aR  A  S  L  H  K  G  L  D  S  A  V  E  V  C  V  V  Y  G  N —TACTCCCAGCAGCTTCAGGTTTACTCAAAAACGGGGTTCAACTGTGATGGGAAATTGGGC  241−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+  300ATGAGGGTCGTCGAAGTCCAAATGAGTTTTTGCCCCAAGTTGACACTACCCTTTAACCCG aY  S  Q  Q  L  Q  V  Y  S  K  T  G  F  N  C  D  G  K  L  G —AATGAATCAGTGACATTCTACCTCCAGAATTTGTATGTTAACCAAACAGATATTTACTTC  301−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+  360TTACTTAGTCACTGTAAGATGGAGGTCTTAAACATACAATTGGTTTGTCTATAAATGAAG aN  E  S  V  T  F  Y  L  Q  N  L  Y  V  N  Q  T  D  I  Y  F —TGCAAAATTGAAGTTATGTATCCTCCTCCTTACCTAGACAATGAGAAGAGCAATGGTACC  361−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+  420ACGTTTTAACTTCAATACATAGGAGGAGGAATGGATCTGTTACTCTTCTCGTTACCATGG aC  K  I  E  V  M  Y  P  P  P  Y  L  D  N  E  K  S  N  G  T —ATTATCCATGTGAAAGGGAAACACCTITGTCCAAGTCCGCTATTTCCCGGACCTTCTAAG  421−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+  480TAATAGGTACACTTTCCCTTTGTGGAAACAGGTTCAGGCGATAAAGGGCCTGGAAGATTC aI  I  H  V  K  G  K  H  L  C  P  S  P  L  F  P  G  P  S  K —CCCCTGGTACCCAGGGGTAGTGGTAGTAAGCCTAGCATAAGTACAGTCCCAGAAGTATCA  481−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+  540GGGGACCATGGGICCCCATCACCATCATTCGGATCGTATTCATGTCAGGGTCTTCATAGT aP  L  V  P  R  G  S  G  S  K  P  S  I  S  T  V  P  E  V  S —TCTGTCTTCATCTTCCCCCCAAAGCCCAAGGATGTGCTCACCATTACTCTGACTCCTAAG  541−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+  600AGACAGAAGTAGAAGGGGGGTTTCGGGTTCCTACACGAGTGGTAATGAGACTGAGGATTC aS  V  F  I  F  P  P  K  P  K  D  V  L  T  I  T  L  T  P  K —GTCACGTGTGTTGTGGTAGACATCAGCAAGGATGATCCCGAGGTCCAGTTCAGCTGGTTT  601−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+  660CAGTGCACACAACACCATCTGTAGTCGTTCCTACTAGGGCTCCAGGTCAAGTCGACCAAA aV  T  C  V  V  V  D  I  S  K  D  D  P  E  V  Q  F  S  W  F —GTAGATGATGTGGAGGTGCACACAGCTCAGACGCAACCCCGGGAGGAGCAGTTCAACAGC  661−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+  720CATCTACTACACCTCCACGTGTGTCGAGTCTGCGTTGGGGCCCTCCTCGTCAAGTTGTCG aV  D  D  V  E  V  H  T  A  Q  T  Q  P  R  E  E  Q  F  N  S —ACTTTCCGCTCAGTCAGTGAACTTCCCATCATGCACCAGGACTGGCTCAATGGCAAGGAG  721−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+  780TGAAAGGCGAGTCAGTCACTTGAAGGGTAGTACGTGGTCCTGACCGAGTTACCGTTCCTC aT  F  R  S  V  S  E  L  P  I  M  H  Q  D  W  L  N  G  K  E —TTCAAATGCAGGGTCAACAGTGCAGCTTTCCCTGCCCCCATCGAGAAAACCATCTCCAAA  781−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+  840AAGTTTACGTCCCAGTTGTCACGTCGAAAGGGACGGGGGTAGCTCTTTTCGTAGAGGTTT aF  K  C  R  V  N  S  A  A  F  P  A  P  I  E  K  T  I  S  K —ACCAAAGGCAGACCGAAGGCTCCACAGGTGTACACCATTCCACCTCCCAAGGAGCAGATG  841−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+  900TGGTTTCCGTCTGGCTTCCGAGGTGTCCACATGTGGTAAGGTGGAGGGTTCCTCGTCTAC aT  K  G  R  P  K  A  P  Q  V  Y  T  I  P  P  P  K  E  Q  M —GCCAAGGATAAAGTCAGTCTGACCTGCATGATAACAGACTTCTTCCCTGAAGACATTACT  901−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+  960CGGTTCCTATTTCAGTCAGACTGGACGTACTATTGTCTGAAGAAGGGACTTCTGTAATGA aA  K  D  K  V  S  L  T  C  M  I  T  D  F  F  P  E  D  I  T —GTGGAGTGGCAGTGGAATGGGCAGCCAGCGGAGAACTACAAGAACACTCAGCCCATCATG  961−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+ 1020CACCTCACCGTCACCTTACCCGTCGGTCGCCTCTTGATGTTCTTGTGAGTCGGGTAGTAC aV  E  W  Q  W  N  G  Q  P  A  E  N  Y  K  N  T  Q  P  I  M —GACACAGATGGCTCTTACTTCGTCTACAGCAAGCTCAATGTGCAGAAGAGCAACTGGGAG 1021−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+ 1080CTGTGTCTACCGAGAATGAAGCAGATGTCGTTCGAGTTACACGTCTTCTCGTTGACCCTC aD  T  D  G  S  Y  F  V  Y  S  K  L  N  V  Q  K  S  N  W  E —GCAGGAAATACTTTCACCTGCTCTGTGTTACATGAGGGCCTGCACAACCACCATACTGAG 1081−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+−−−−−−−−−+ 1140CGTCCTTTATGAAAGTGGACGAGACACAATGTACTCCCGGACGTGTTGGTGGTATGACTC aA  G  N  T  F  T  C  S  V  L  H  E  G  L  H  N  H  H  T  E —AAGAGCCTCTCCCACTCTCCTGGTAAATAA 1141 −−−−−−−−−+−−−−−−−−−+−−−−−−−−−+ 1170TTCTCGGAGAGGGTGAGAGGACCATTTATT a K  S  L  S  H  S  P  G  K  * —

1. A composition of matter comprising: (a) an isolated antibody thatinduces superagonistic signaling by a cell surface receptor, whereinsaid antibody binds to the extracellular portion of the receptor at amembrane proximal region and said receptor comprises a cytoplasmicdomain which is dependent on an extrinsic protein kinase, wherein saidantibody does not bind only the C′-D loop of human CD28; (b) an isolatedchimeric protein that induces superagonistic signaling by a cell surfacereceptor, which chimeric protein comprises: (i) an amino acid sequencecomprising a fragment of a ligand of the receptor, or a homologue ofsuch a fragment, wherein the fragment or homologue is capable of bindingto the extracellular portion of the receptor at a membrane proximalregion, and (ii) an Fc region of an antibody, wherein said receptorcomprises a cytoplasmic domain which is dependent on an extrinsicprotein kinase; (c) an isolated chimeric protein that inducessuperagonistic signaling by one or two types of cell surface receptor,said chimeric protein comprising two Fv regions of an antibody that maybe the same or different, wherein at least one of the Fv regions iscapable of binding to a first receptor, and the other Fv region eitherbinds to (i) said first receptor, or (ii) a second type of cell surfacereceptor which is found on a cell that contacts a cell which expressessaid first receptor, wherein said first receptor, and optionally alsosaid second receptor, comprises a cytoplasmic domain which is dependenton an extrinsic protein kinase; (d) an isolated peptide of 5 to 20 aminoacids comprising a sequence that binds to an antibody that: (A) thatinduces superagonistic signaling by a cell surface receptor, whereinsaid antibody binds to the extracellular portion of the receptor at amembrane proximal region and said receptor comprises a cytoplasmicdomain which is dependent on an extrinsic protein kinase, wherein saidantibody does not bind only the C′-D loop of human CD28; (B) an isolatedchimeric protein that induces superagonistic signaling by a cell surfacereceptor, which chimeric protein comprises: (i) an amino acid sequencecomprising a fragment of a ligand of the receptor, or a homologue ofsuch a fragment, wherein the fragment or homologue is capable of bindingto the extracellular portion of the receptor at a membrane proximalregion, and (ii) an Fc region of an antibody, wherein said receptorcomprises a cytoplasmic domain which is dependent on an extrinsicprotein kinase; or (C) an isolated chimeric protein that inducessuperagonistic signaling by one or two types of cell surface receptor,said chimeric protein comprising two Fv regions of an antibody that maybe the same or different, wherein at least one of the Fv regions iscapable of binding to a first receptor, and the other Fv region eitherbinds to (i) said first receptor, or (ii) a second type of cell surfacereceptor which is found on a cell that contacts a cell which expressessaid first receptor, wherein said first receptor, and optionally alsosaid second receptor, comprises a cytoplasmic domain which is dependenton an extrinsic protein kinase; (e) a crystal comprising (i) CD28; (ii)a fragment of CD28; or (iii) a homologue of CD28 or a fragment thereof;(f) a crystal comprising a CD28 specific antibody or an antigen bindingfragment of said antibody bound to: (i) CD28; (ii) a fragment of CD28;or (iii) a homologue of CD28 or a fragment of thereof; (g) a crystalhaving the structural coordinates shown in Table 4; (h) machine-readabledata storage medium comprising a data storage material encoded with amachine readable data which when read by an appropriate machine displaysa representation of a crystal: (A) comprising (i) CD28; (ii) a fragmentof CD28; or (iii) a homologue of CD28 or a fragment thereof; (B)comprising a CD28 specific antibody or a fragment of said antibody boundto:(i) CD28; (ii) a fragment of CD28; or (iii) a homologue of CD28 or afragment thereof; or (C) having the structural coordinates shown inTable 4; or (i) a computer program product comprising a program codemeans stored on a computer readable storage medium for comparing astructural model of a candidate modulator with a structural model ofCD28 to thereby determine whether the modulator will bind to CD28,wherein the structural model is derived from, or comprises, structuralcoordinates of a crystal of: (i) CD28, (ii) a fragment of CD28, or (iii)a homologue of CD28 or a fragment of CD28.
 2. The composition of matteraccording to claim 1, wherein said antibody or chimeric protein: (i)binds orthogonally to the main axis of the domain of the receptor whichit is binding; and/or (ii) lies parallel to the cell surface when boundto the receptor; and/or (iii) binds to a β-strand polypeptide chain ofthe receptor; and/or (iv) binds within 75 Å of the cell surface.
 3. Thecomposition of matter according to claim 1, wherein said antibody orchimeric protein binds to a sequence as shown in Table 1 or anequivalent homologous sequence in the proximal membrane region of areceptor which is capable of being induced to signal by the antibody orchimeric protein.
 4. The composition of matter according to claim 2,wherein said antibody or chimeric protein binds to a sequence as shownin Table 1 or an equivalent homologous sequence in the proximal membraneregion of a receptor which is capable of being induced to signal by theantibody or chimeric protein.
 5. The composition of matter according toclaim 1, wherein said receptor: (i) comprises an ITAM motif, ITIM motifor “switch” signaling motif; and/or (ii) is a member of the CD28 familyof proteins; and/or (iii) is expressed on the surface of a cell of theimmune system; and/or (iv) comprises a cytoplasmic domain capable ofbeing phosphorylated by a Src kinase; and/or (v) comprises a cytoplasmicdomain capable of being dephosphorylated by CD45; and/or (vi) is one ofthe receptors listed in Table 2, or is a homologue thereof.
 6. Thecomposition of matter according to claim 2, wherein said receptor (i)comprises an ITAM motif, ITIM motif or “switch” signaling motif; and/or(ii) is a member of the CD28 family of proteins; and/or (iii) isexpressed on the surface of a cell of the immune system; and/or (iv)comprises a cytoplasmic domain capable of being phosphorylated by a Srckinase; and/or (v) comprises a cytoplasmic domain capable of beingdephosphorylated by CD45; and/or (vi) is one of the receptors listed inTable 2, or is a homologue thereof.
 7. The composition of matteraccording to claim 3, wherein said receptor: (i) comprises an ITAMmotif, ITIM motif or “switch” signaling motif; and/or (ii) is a memberof the CD28 family of proteins; and/or (iii) is expressed on the surfaceof a cell of the immune system; and/or (iv) comprises a cytoplasmicdomain capable of being phosphorylated by a Src kinase; and/or (v)comprises a cytoplasmic domain capable of being dephosphorylated byCD45; and/or (vi) is one of the receptors listed in Table 2, or is ahomologue thereof.
 8. The composition of matter according to claim 4,wherein said receptor: (i) comprises an ITAM motif, ITIM motif or“switch” signaling motif; and/or (ii) is a member of the CD28 family ofproteins; and/or (iii) is expressed on the surface of a cell of theimmune system; and/or (iv) comprises a cytoplasmic domain capable ofbeing phosphorylated by a Src kinase; and/or (v) comprises a cytoplasmicdomain capable of being dephosphorylated by CD45; and/or (vi) is one ofthe receptors listed in Table 2, or is a homologue thereof.
 9. A methodof identifying a candidate modulator or a candidate agent that modulatesa receptor comprising: A) determining whether a candidate agent binds toa membrane proximal extracellular region of the receptor, to therebydetermine whether the candidate agent is capable of superagonizing thereceptor; or B) identifying a modulator of CD28 comprising comparing astructural model of a candidate modulator with a structural model ofCD28 to thereby determine whether the modulator will bind to CD28,wherein the structural model is derived from, or comprises, structuralcoordinates of a crystal of: (i) CD28, (ii) a fragment of CD28, or (iii)a homologue of CD28 or a homologue of a fragment of CD28; wherein saidreceptor (i)comprises an ITAM motif, ITIM motif or “switch” signalingmotif, and/or (ii) is a member of the CD28 family of proteins, and/or(iii) is expressed on the surface of a cell of the immune system, and/or(iv) comprises a cytoplasmic domain capable of being phosphorylated by aSrc kinase, and/or (v) comprises a cytoplasmic domain capable of beingdephosphorylated by CD45, and/or (vi) is one of the receptors listed inTable 2, or is a homologue thereof and/or (vii) comprises a cytoplasmicdomain which is dependent on an extrinsic protein kinase.
 10. The methodaccording to claim 9, wherein said method comprises determining whethera candidate agent which binds to the receptor or fails to bind to amutated version of the receptor wherein one or more amino acids in amembrane proximal extracellular region of the receptor have beenmutated, said failure to bind to the mutant receptor indicating that theagent is capable of inducing superagonistic signaling by the receptor.11. The method according to claim 10, wherein the method is performed bycontacting the candidate agent with (i) a full length receptor with saidmutations, or (ii) a homologue of a full length receptor with saidmutations, or (iii) a fragment of (i) or (ii) comprising said mutations.12. The method according to claim 9, wherein the location of the bindingof the candidate agent is determined by contacting the candidate agentwith: (i) a peptide comprising sequence from a membrane proximalextracellular region of the receptor that is at least five amino acidsin length; or (ii) an array of overlapping peptides that representfragments of the receptor and are 5 to 20 amino acids in length anddetermining whether the candidate agent binds to the peptide or apeptide in the array of overlapping peptides, the binding of thecandidate agent to a peptide indicating that the agent is capable ofinducing superagonistic signaling of said receptor.
 13. The methodaccording to claim 9, wherein said candidate agent is an antibody. 14.The method according to claim 9, wherein said comparison comprisesfitting (docking) the structural model of the candidate modulator withthe structural model of CD28, and optionally determining the bindingfree energy of binding between the candidate modulator and CD28, whereina low (more negative) binding free energy indicates that the candidateis likely to bind to CD28.
 15. The method according to claim 14, whereinthe binding free energy is calculated by (i) summing the free energiesof interatomic contacts between the structural model of the candidatemodulator and the structural model of CD28, or (ii) determining the freebinding energy between the force field of the candidate modulator andthe force field of CD28.
 16. The method according to claim 9, whereindetermining whether or not the candidate modulator binds to CD28comprises: (i) comparing the fitting of the structural model of thecandidate modulator and the structural model of CD28 with the fitting ofa structural model of another protein bound to a ligand, to therebydetermine whether or not the candidate modulator will bind to CD28; or(ii) comparing the fitting of the structural model of the candidatemodulator and the structural model of CD28 with the fitting of astructural model of another protein bound to a ligand, to therebydetermine whether or not the candidate modulator will bind to CD28,wherein the structural coordinates are obtainable by subjecting acrystal of (A) CD28, (B) a fragment of CD28, or (C) a homologue of (A)or (B), to X-ray diffraction measurements and deducing the structuralcoordinates from the diffraction measurements.
 17. The method accordingto claim 9, wherein: A) the crystal is: i) CD28, (ii) a fragment ofCD28, or (iii) a homologue of CD28 or a homologue of a fragment of CD28bound to a CD28 specific antibody or an antigen binding fragment of saidantibody; or (ii) the crystal has the structural coordinates shown inTable
 4. 18. The method according to claim 9, further comprisingcontacting the identified modulator of CD28 with (i) CD28, (ii) afragment of CD28, or (iii) a homologue of CD28 or a fragment thereof todetermine whether or not the modulator is capable of binding, ormodulating the activity of CD28.
 19. A method of modulating the immuneresponse of a patient or of inducing superagonistic signaling by areceptor comprising: (i) administering a modulator of CD28 to saidpatient; or (ii) administering an antibody or chimeric protein thatinduces superagonistic signaling by a cell surface receptor, whereinsaid antibody or chimeric protein binds to the extracellular portion ofthe receptor at a membrane proximal region and said receptor comprises acytoplasmic domain which is dependent on an extrinsic protein kinase,wherein said antibody or chimeric protein does not bind only the C′-Dloop of human CD28 to said patient; or (iii) administering an agent thatbinds to a membrane proximal extracellular region of the receptor andhas the ability to superagonize the receptor to said patient; or (iv)administering a peptide that stimulates an antibody response in thepatient wherein the antibody binds to the extracellular portion of thereceptor at a membrane proximal region and said receptor comprises acytoplasmic domain which is dependent on an extrinsic protein kinase andwherein said antibody does not bind only the C′-D loop of human CD28; or(v) administering a nucleic acid capable of expressing (i), (ii), (iii)or (iv) to said patient; or (vi) administering an agent or modulator toa patient that sterically inhibits contact between a phosphatase of thecell and the receptor, excluding a method in which an antibody thatbinds only the C′-D loop of CD28 is used to sterically inhibit contactbetween CD28 and the phosphatase CD4.